Dietary rumen-protected L-arginine or N-carbamylglutamate attenuated fetal hepatic inflammation in undernourished ewes suffering from intrauterine growth restriction

作     者：Hao Zhang Ying Zhang Yi Ma Mabrouk Elsabagh Hongrong Wang Mengzhi Wang Hao Zhang;Ying Zhang;Yi Ma;Mabrouk Elsabagh;Hongrong Wang;Mengzhi Wang

作者机构：State Key Laboratory of Sheep Genetic Improvement and Healthy ProductionXinjiang Academy of Agricultural Reclamation SciencesShihezi832000China Joint International Research Laboratory of Agriculture and Agri-Product SafetyThe Ministry of Education of ChinaYangzhou UniversityYangzhou225009China Laboratory of Metabolic Manipulation of Herbivorous Animal NutritionCollege of Animal Science and TechnologyYangzhou UniversityYangzhou225009China Department of Animal Production and TechnologyFaculty of Agricultural Sciences and TechnologiesNigde Omer Halisdemir UniversityNigde51240Turkey Department of Nutrition and Clinical NutritionFaculty of Veterinary MedicineKafrelsheikh UniversityKafrelsheikh33516Egypt 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2021年第7卷第4期

页      面：1095-1104页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 0906[农学-兽医学] 

基　　金：supported by the fund for the National Natural Science Foundation of China(31902180) the Top Talents Award Plan of Yangzhou University(2019) the Cyanine Project of Yangzhou University(2020) the funds from State Key Laboratory of Sheep Genetic Improvement and Healthy Production(2021ZD07) Yangzhou University Science and Technology Innovation Foundation(2019CXJ152)。 

主　　题：L-arginine Fetal sheep Liver inflammation Intrauterine growth restriction N-carbamylglutamate 

摘      要：This study aimed to explore whether dietary rumen-protected L-arginine(RP-Arg)or N-carbamylglutamate(NCG)supplementation to feed-restricted pregnant ewes counteracts fetal hepatic inflammation and innate immune dysfunction associated with intrauterine growth retardation(IUGR)in ovine fetuses.On d 35 of pregnancy,twin-bearing Hu ewes(n=32)were randomly assigned to 4 treatment groups(8 ewes and 16 fetuses per group)and fed diets containing 100%of the NRC requirements(CON),50%of the NRC requirements(RES),RES+RP-Arg(20 g/d)(RESA),or RES+NCG(5 g/d)(RESN).At 08:00 on d 110 of gestation,fetal blood and liver tissue samples were collected.The levels of triglyceride,free fatty acid,cholesterol andβ-hydroxybutyrate in the fetal blood of RESA and RESN groups were lower(P0.05)than those of the RES group,but were higher(P0.05)than those of the CON group.The interleukin(IL)-6 and IL-1 levels in fetal blood and liver tissue as well as the myeloid differentiation primary response 88(MyD88),transforming growth factorβ(TGFβ),and nuclear factor kappa B(NF-κB)mRNA levels in the fetal liver were decreased(P0.05)by the NCG or RP-Arg supplementation compared to the RES treatment.Similarly,the toll-like receptor(TLR)-4,MyD88,TGFβ,and p-c-Jun N-terminal kinase(JNK)protein levels in the fetal liver were reduced(P0.05)in the NCG and RP-Arg-supplemented groups compared to the RES group.These results showed that dietary supplementation of RP-Arg or NCG to underfed pregnant ewes could protect against IUGR fetal hepatic inflammation via improving lipid metabolism,down-regulating the TLR-4 and the inflammatory JNK and NF-icB signaling pathways,and decreasing cytokine production in ovine fetal blood and liver tissue.