Folding patterns of chromatin in chicken erythrocyte studied by using AFM and TEM

作     者：Liu, ZX Zhou, MY Xie, HY Qian, RL Zhang, Y Hu, J Li, MQ 

作者机构：Chinese Acad Sci Shanghai Inst Cell Biol Shanghai 200031 Peoples R China Chinese Acad Sci Shanghai Inst Nucl Res Shanghai 201800 Peoples R China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：1998年第43卷第4期

页      面：289-291页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 0905[农学-畜牧学] 09[农学] 071007[理学-遗传学] 0836[工学-生物工程] 090501[农学-动物遗传育种与繁殖] 

基　　金：ThisworkwassupportedbytheFoundationofChineseAcademyofSciences (GrantNo .KJ95 1_Al_6 0 3)andShanghaiOpenLaboratoryofLifeSciences 

主　　题：chromatin structure nucleus of chicken erythrocyte AFM TEM. 

摘      要：Four folding patterns (～15, ～30-40, ～60 and ～90-110 nm) of the higher_order structure of chromatin in chicken erythrocytes have been observed with an atomic force microscope (AFM) under near natural conditions. Results showed that the chromatin in the interphase nucleus of chicken erythrocyte was not in a disorganized form but represented a higher_order conformation. The thin sections of the chromatin in the nucleus of chicken erythrocyte were examined with a transmission electron microscope (TEM). Compared with TEM, AFM had the advantages that the samples did not need the preparation procedures such as fixing, sectioning and staining, and thus were closer to their native condition.