Experimental study on rehydration conditions of freeze-dried platelets

作     者：Ju-li FAN Xian-guo XU Shao-zhi ZHANG Fa-ming ZHU Guang-ming CHEN Li-xing YAN 

作者机构：Institute of Refrigeration and Cryogenics Zhejiang Universit Hangzhou 310027 China Key Laboratory of Blood Safety Research Ministry of Health Blood Center of Zhejiang Province Hangzhou 310006 China 

出 版 物：《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 (浙江大学学报（英文版）A辑（应用物理与工程）)

年 卷 期：2009年第10卷第5期

页      面：697-703页

核心收录：

学科分类：0710[理学-生物学] 0810[工学-信息与通信工程] 0832[工学-食品科学与工程（可授工学、农学学位）] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 083201[工学-食品科学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：supported by the National Natural Science Foundation of China (No. 50606032) the Science Foundation of Health Office of China (No. WKJ2005-2-037) 

主　　题：Freeze-drying Platelets Rehydration Prehydration Rehydration solution 

摘      要：A rehydration process for freeze-dried human platelets was studied on 1 ml of samples. The effects of prehydration duration, prehydration temperature, an rehydration solution on the recovery rate, mean platelet volume （MPV）, and platelet distribution width （PDW） were investigated. The mass changes during the prehydration process were also studied. Three prehydration durations： 0, 1.5, and 3.5 h, and two rehydration solutions： platelet-poor plasma and phosphate-buffered saline （PBS）, were tested. It was found that： （1） the prehydration was of significance; （2） 1.5 h of prehydration had better effects than 3.5 h of prehydration; （3） as a rehydration solution, the platelet-poor plasma behaved better than the PBS. The impacts of prehydration duration and temperature on the results were studied. There was almost no difference between 35 and 37 ℃. Among all the prehydration durations tested, 15, 30, 60, 90, and 120 min, the best result was achieved with the time duration of 15 min. The weights of prehydrated platelets at the end of each test were measured and the water contents were calculated. After 15 min ofprehydration, the water contents in the samples were about （4.8±0.01）% and （5.27±0.29）% （w/w） corresponding to the conditions of 35 and 37 ℃, respectively. These results will be helpful for further studies on the freeze-drying of mammalian cells.