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Experimental study on rehydration conditions of freeze-dried platelets

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2009年第5期10卷 697-703页

作者： Ju-li FAN Xian-guo XU Shao-zhi ZHANG Fa-ming ZHU Guang-ming CHEN Li-xing YAN Institute of Refrigeration and Cryogenics Zhejiang Universit Hangzhou 310027 China Key Laboratory of Blood Safety Research Ministry of Health Blood Center of Zhejiang Province Hangzhou 310006 China

A rehydration process for freeze-dried human platelets was studied on 1 ml of samples. The effects of prehydration duration, prehydration temperature, an rehydration solution on the recovery rate, mean platelet volume （MPV）, and platelet distribution width （PDW） were investigated. The mass changes during the prehydration process were also studied. Three prehydration durations： 0, 1.5, and 3.5 h, and two rehydration solutions： platelet-poor plasma and phosphate-buffered saline （PBS）, were tested. It was found that：（1） the prehydration was of significance; （2） 1.5 h of prehydration had better effects than 3.5 h of prehydration; （3） as a rehydration solution, the platelet-poor plasma behaved better than the PBS. The impacts of prehydration duration and temperature on the results were studied. There was almost no difference between 35 and 37 ℃. Among all the prehydration durations tested, 15, 30, 60, 90, and 120 min, the best result was achieved with the time duration of 15 min. The weights of prehydrated platelets at the end of each test were measured and the water contents were calculated. After 15 min ofprehydration, the water contents in the samples were about （4.8±0.01）% and （5.27±0.29）% （w/w） corresponding to the conditions of 35 and 37 ℃, respectively. These results will be helpful for further studies on the freeze-drying of mammalian cells.

关键词： Freeze-drying Platelets Rehydration prehydration Rehydration solution

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Optimization study on the rehydration process of lyophilized human platelets

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Science Bulletin 2011年第Z1期 455-460页

作者： FAN JuLi1,2,XU XianGuo3,4,ZHANG ShaoZhi2,XU MengJie2,ZHU FaMing3,4 & CHEN GuangMing2 1 Department of Man-machine and Environment Engineering,Nanjing University of Aeronautics and Astronautics,Nanjing 210016,China 2 Institute of Refrigeration and Cryogenics,Zhejiang University,Hangzhou 310027,China 3 Blood Center of Zhejiang Province,Hangzhou 310006,China 4 Key Laboratory of Blood Safety Research,Ministry of Health,Hangzhou 310006,China

Long-term preservation of human platelets will greatly reduce the risk of their shortage. Lyophilization has been proved feasible for this purpose. For the recovery of lyophilized platelets,rehydration is an important process. In this paper,the rehydration proc-esses for 1 mL and 2 mL samples were studied. The effects of prehydration duration(15,30,60,90,120 and 150 min) in 37°C water vapor and the concentration of rehydration solution(25%,50%,75%,100% platelet-poor plasma) on the recovery rate,MPV(mean platelet volume) and PDW(platelet distribution width) were investigated. The mass changes during the prehydration process were weighed. The optimized rehydration conditions are as follows:(1) for 1 mL sample,the prehydration duration was 15 min and for 2 mL sample the prehydration duration was 90 min;(2) the rehydration solution was 75% platelet-poor plasma. Under optimized conditions,the morphology of the rehydrated platelets kept normal and their ultrastructures kept intact,their aggregation capacity to thrombin(1 U/mL) was 82.8% of the fresh ones. These results will be helpful for designing the freeze-drying protocols for human platelets.

关键词： lyophilization human platelets prehydration rehydration solution morphology aggregation function

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