Controlled temperature-mediated curcumin release from magneto-thermal nanocarriers to kill bone tumors

作     者：A.Khodaei F.Jahanmard H.R.Madaah Hosseini R.Bagheri A.Dabbagh H.Weinans Amin Yavari 

作者机构：Institute for nanoscience and nanotechnologysharif university of technologytehraniran department of orthopedicsuniversity medical center utrechtutrechtthe netherlands department of materials science and engineeringsharif university of technologytehraniran school of medicinefaculty of health and medical sciencestaylor’s universitysubang jayamalaysia regenerative medicine utrechtutrecht universityutrechtthe netherlands 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2022年第7卷第5期

页      面：107-117页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 100214[医学-肿瘤学] 0836[工学-生物工程] 10[医学] 

主　　题：Osteosarcoma Magnetic hyperthermia Curcumin MRI contrast agent 

摘      要：Systemic chemotherapy has lost its position to treat cancer over the past years mainly due to drug resistance,side effects,and limited survival *** a plethora of local drug delivery systems to solve this issue,the combinatorial strategy of chemo-hyperthermia has recently received *** we developed a magneto-thermal nanocarrier consisted of superparamagnetic iron oxide nanoparticles(SPIONs)coated by a blend formulation of a three-block copolymer Pluronic F127 and F68 on the oleic acid(OA)in which Curcumin as a natural and chemical anti-cancer agent was *** subsequent nanocarrier SPION@OA-F127/F68-Cur was designed with a controlled gelation temperature of the shell,which could consequently control the release of *** release was systematically studied as a function of temperature and pH,via response surface methodology(RSM).The bone tumor killing efficacy of the released curcumin from the carrier in combination with the hyperthermia was studied on MG-63 osteosarcoma cells through Alamar blue assay,live-dead staining and apoptosis caspase 3/7 activation *** was found that the shrinkage of the F127/F68 layer stimulated by elevated temperature in an alternative magnetic field caused the curcumin *** the maximum release concentration and cell death took place at 45℃,treatment at 41℃ was chosen as the optimum condition due to considerable cell apoptosis and lower side effects of mild *** cell metabolic activity results confirmed the synergistic effects of curcumin and hyperthermia in killing MG-63 osteosarcoma cells.