Prime Editing Technology and Its Prospects for Future Applications in Plant Biology Research
作者机构：Biosciences DivisionOak Ridge National LaboratoryOak Ridge TN 37831USA Center for Bioenergy InnovationOak Ridge National LaboratoryOak RidgeTN 37831USA Department of Genetics and Plant BreedingPatuakhali Science and Technology UniversityDumkiPatuakhali 8602Bangladesh
出 版 物：《BioDesign Research》 (生物设计研究（英文）)
年 卷 期：2020年第2020卷第1期
页 面：124-137页
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学]
基 金：This manuscript has been authored by UT-Battelle,LLC,under Contract No.DE-AC05-00OR22725 with the U.S Department of Energy,Oak Ridge National Laboratory is managed by UT-Battelle,LLC,for the U.S.Department of Energy under Contract Number DEAC05-00OR22725
摘 要：Many applications in plant biology requires editing genomes accurately including correcting point mutations,incorporation of single-nucleotide polymorphisms(SNPs),and introduction of multinucleotide insertion/deletions(indels)into a predetermined position in the *** types of modifications are possible using existing genome-editing technologies such as the CRISPR-Cas systems,which require induction of double-stranded breaks in the target DNA site and the supply of a donor DNA molecule that contains the desired edit ***,low frequency of homologous recombination in plants and difficulty of delivering the donor DNA molecules make this process extremely *** kind of technology known as base editing can perform precise editing;however,only certain types of modifications can be obtained,e.g.,C/G-to-T/A and A/T-to-G/***,a new type of genome-editing technology,referred to as“prime editing,has been developed,which can achieve various types of editing such as any base-to-base conversion,including both transitions(C→T,G→A,A→G,and T→C)and transversion mutations(C→A,C→G,G→C,G→T,A→C,A→T,T→A,and T→G),as well as small indels without the requirement for inducing double-stranded break in the *** prime editing has wide flexibility to achieve different types of edits in the genome,it holds a great potential for developing superior crops for various purposes,such as increasing yield,providing resistance to various abiotic and biotic stresses,and improving quality of plant *** this review,we describe the prime editing technology and discuss its limitations and potential applications in plant biology research.
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