Melatonin treatment induces chilling tolerance by regulating the contents of polyamine,γ-aminobutyric acid,and proline in cucumber fruit

作     者：Miilion P MADEBO LUO Si-ming WANG Li ZHENG Yong-hua JIN Peng Miilion P MADEBO;LUO Si-ming;WANG Li;ZHENG Yong-hua;JIN Peng

作者机构：College of Food Science and TechnologyNanjing Agricultural UniversityNanjing 210095P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2021年第20卷第11期

页      面：3060-3074页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 090202[农学-蔬菜学] 

基　　金：supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions and Program for Student Innovation through Research and Training(SRT) China(1918C12) 

主　　题：cucumber melatonin polyamine proline GABA chilling injury 

摘      要：The mechanism of melatonin(MT)induced chilling tolerance in harvested cucumber fruit was investigated at commercial *** this study,cucumber fruits were treated with 100μmol L^(-1) MT at 4℃ and 90% relative humidity for 15 d of *** comparison with the control,cucumber treatment with MT resulted in reduced chilling injury(CI),decreased electrolyte leakage and enhanced *** fruits treated with MT showed higher chlorophyll contents in storage conditions with suppressed chlorophyllase enzyme *** treatment increased arginine decarboxylase(ADC)and ornithine decarboxylase(ODC)enzyme ***,enhanced expression of the Cucumis sativus ADC(CsADC)and *** ODC(CsODC)genes resulted in the accumulation of polyamine ***,proline levels exhibited higher levels among treated ***,the proline synthesizing enzymes △1-pyrroline-5-carboxylate syntheses(P5CS)and ornithine aminotransferase(OAT)were significantly increased,while a catabolic enzyme of proline dehydrogenase(PDH)activity was inhibited by *** addition,MT induced expression of *** OAT(CsOAT)and *** P5CS(CsP5CS)*** fruits treated with MT also exhibited higher γ-aminobutyric acid(GABA)content by enhanced GABA transaminase(GABA-T)and glutamate decarboxylase(GAD)enzyme activities and a higher *** GAD(CsGAD)gene *** sum up,the results show that MT treatment enhanced chilling tolerance,which was associated with the regulation of polyamines,as well as proline and γ-aminobutyric acid.