检索结果-南通市图书馆

Nano Research 2023年第2期16卷 2885-2894页

作者： Lei Zhang Jiaru Jing Lin Han Ziyan Liu Jingyu Wang Wei Zhang Ai Gao Department of Occupational Health and Environmental Health School of Public HealthCapital Medical UniversityBeijing 100069China Beijing Key Laboratory of Environmental Toxicology Capital Medical UniversityBeijing 100069China

Plastic pollution has become a non-negligible global pollution problem.Nanoplastics(NP)can reach the bone marrow with blood circulation and develop hematotoxicity,but potential mechanisms and prevention strategies are lacking.Here,we report the biological distribution of NP particles in the bone marrow of mice and hematopoietic toxicity after exposure to 60μg of 80 nm NP for 42 days.NP exposure inhibited the capability of bone marrow hematopoietic stem cells to renew and differentiate.Notably,probiotics and melatonin supplementation significantly ameliorated NP-induced hematopoietic damage,and the former was superior to the latter.And interestingly,melatonin and probiotic interventions may involve different microbes and metabolites.After melatonin intervention,creatine showed a stronger correlation with NP-induced gut microbiota disorders.In contrast,probiotic intervention reversed the levels of more gut microbes and plasma metabolites.Of these,threonine,malonylcarnitine,and 3-hydroxybutyric acid might be potential performers in the regulation of hematopoietic toxicity by gut microbes,as they had a more significant relationship with the identified microbes.In conclusion,supplementation with melatonin or probiotics may be two candidates to prevent hematopoietic toxicity attributable to NP exposure.Also,the multi-omics results may lay the foundation for future investigations into in-depth mechanisms.

关键词： gut microbiota-metabolism melatonin probiotics nanoplastics exposure hematopoietic injury

melatonin decreases GSDME mediated mesothelial cell pyroptosis and prevents peritoneal fibrosis and ultrafiltration failure

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2024年第2期67卷 360-378页

作者： Hongxia Ruan Xuejuan Li Lina Zhou Zihan Zheng Rulin Hua Xu Wang Yuan Wang Yujie Fan Shuwen Guo Lihua Wang Shafiq ur Rahman Ziwei Wang Yuyuan Wei Shuangyan Yu Rongzhi Zhang Qian Cheng Jie Sheng Xue Li Xiaoyan Liu Ruqiang Yuan Xiaoyan Zhang Lihong Chen Guowang Xu Youfei Guan Jing Nie Hongqiang Qin Feng Zheng Advanced Institute for Medical Sciences Dalian Medical UniversityDalian 116044China Department of Nephrology Second HospitalDalian Medical UniversityDalian 116023China CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China Chongqing International Institute for Immunology Chongqing 401320China Department of Nephrology Shenzhen HospitalSouthern Medical UniversityShenzhen 518101China Wuhu Hospital and Health Science Center East China Normal UniversityShanghai 200241China Peking University First Hospital Peking UniversityBeijing 100034China

Peritoneal fibrosis together with increased capillaries is the primary cause of peritoneal dialysis failure.Mesothelial cell loss is an initiating event for peritoneal fibrosis.We find that the elevated glucose concentrations in peritoneal dialysate drive mesothelial cell pyroptosis in a manner dependent on caspase-3 and Gasdermin E,driving downstream inflammatory responses,including the activation of macrophages.Moreover,pyroptosis is associated with elevated vascular endothelial growth factor A and C,two key factors in vascular angiogenesis and lymphatic vessel formation.GSDME deficiency mice are protected from high glucose induced peritoneal fibrosis and ultrafiltration failure.Application of melatonin abrogates mesothelial cell pyroptosis through a MT1R-mediated action,and successfully reduces peritoneal fibrosis and angiogenesis in an animal model while preserving dialysis efficacy.Mechanistically,melatonin treatment maintains mitochondrial integrity in mesothelial cells,meanwhile activating m TOR signaling through an increase in the glycolysis product dihydroxyacetone phosphate.These effects together with quenching free radicals by melatonin help mesothelial cells maintain a relatively stable internal environment in the face of high-glucose stress.Thus,melatonin treatment holds some promise in preserving mesothelium integrity and in decreasing angiogenesis to protect peritoneum function in patients undergoing peritoneal dialysis.

关键词： melatonin mitochondria pyroptosis GSDME peritoneal fibrosis mTOR

melatonin promotes the development of the secondary hair follicles by regulating circMPP5

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2023年第4期14卷 1696-1712页

作者： Xiaogao Diao Lingyun Yao Tao Duan Jiaxin Qin Liwen He Wei Zhang Department of Animal Nutrition and Feed Science State Key Laboratory of Animal NutritionCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Background The quality and yield of cashmere fibre are closely related to the differentiation and development of secondary hair follicles in the skin of cashmere goats.The higher the density of secondary hair follicles,the higher the quality and yield of cashmere from the fleece.Development of secondary hair follicles commences in the embryonic stage of life and is completed 6 months after birth.Preliminary experimental results from our laboratory showed that melatonin(MT)treatment of goat kids after their birth could increase the density of secondary hair follicles and,thus,improve the subsequent yield and quality of cashmere.These changes in the secondary hair follicles resulted from increases in levels of antioxidant and expression of anti-apoptotic protein,and from a reduction in apoptosis.The present study was conducted to explore the molecular mechanism of MT-induced secondary hair follicle differentiation and development by using whole-genome analysis.Results MT had no adverse effect on the growth performance of cashmere kids but significantly improved the character of the secondary hair follicles and the quality of cashmere,and this dominant effect continued to the second year.melatonin promotes the proliferation of secondary hair follicle cells at an early age.The formation of secondary hair follicles in the MT group was earlier than that in the control group in the second year.The genome-wide data results involved KEGG analysis of 1044 DEmRNAs,91 DElncRNAs,1054 DEcircRNAs,and 61 DEmiRNAs which revealed that the mitogen-activated protein kinase(MAPK)signaling pathway is involved in the development of secondary hair follicles,with key genes(FGF2,FGF21,FGFR3,MAPK3(ERK1))being up-regulated and expressed.We also found that the circMPP5 could sponged miR-211 and regulate the expression of MAPK3.Conclusions We conclude that MT achieves its effects by regulating the MAPK pathway through the circMPP5 sponged the miR-211,regulating the expression of MAPK3,to induce the differentiation and proliferation of seconda

关键词： Cashmere MAPK melatonin miR-211 Secondary hair follicle

melatonin inhibits ESCC tumor growth by mitigating the HDAC7/β-catenin/c-Myc positive feedback loop and suppressing the USP10-maintained HDAC7 protein stability

在线全文

学校读者我要写书评

暂无评论

Military Medical Research 2023年第2期10卷 207-226页

作者： Zhi-Qiang Ma Ying-Tong Feng Kai Guo Dong Liu Chang-Jian Shao Ming-Hong Pan Yi-Meng Zhang Yu-Xi Zhang Di Lu Di Huang Fan Zhang Jin-Liang Wang Bo Yang Jing Han Xiao-Long Yan Yi Hu Department of Medical Oncology Senior Department of Oncologythe Fifth Medical CenterChinese PLA General HospitalBeijing 100853China Department of Thoracic Surgery Tangdu Hospitalthe Fourth Military Medical UniversityXi’an 710038China Department of Cardiothoracic Surgery the 71th Group Army Hospital of PLAthe Affiliated Huaihai Hospital of Xuzhou Medical UniversityXuzhou 221004JiangsuChina Department of Thoracic Surgery Shaanxi Provincial People’s Hospitalthe Third Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710068China State Key Laboratory of Cardiovascular Disease Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical SciencesPeking Union Medical CollegeBeijing 100037China Department of Ophthalmology Tangdu Hospitalthe Fourth Military Medical UniversityXi’an 710038China Department of Cardiovascular Surgery Xijing Hospitalthe Fourth Military Medical UniversityXi’an 710032China

Background:melatonin,a natural hormone secreted by the pineal gland,has been reported to exhibit antitumor properties through diverse mechanisms of action.However,the oncostatic function of melatonin on esophageal squamous cell carcinoma(ESCC) remains elusive.This study was conducted to investigate the potential effect and underlying molecular mechanism of melatonin as single anticancer agent against ESCC cells.Methods:ESCC cell lines treated with or without melatonin were used in this study.In vitro colony formation and 5-Ethynyl-2’-deoxyuridine(EdU) incorporation assays,and nude mice tumor xenograft model were used to confirm the proliferative capacities of ESCC cells.RNA-seq,qPCR,Western blotting,recombinant lentivirus-mediated target gene overexpression or knockdown,plasmids transfection and co-IP were applied to investigate the underlying molecular mechanism by which melatonin inhibited ESCC cell growth.IHC staining on ESCC tissue microarray and further survival analyses were performed to explore the relationship between target genes’ expression and prognosis of ESCC.Results:melatonin treatment dose-dependently inhibited the proliferative ability and the expression of histone deacetylase 7(HDAC7),c-Myc and ubiquitin-specific peptidase 10(USP10) in ESCC cells(P<0.05).The expressions of HDAC7,c-Myc and USP10 in tumors were significantly higher than the paired normal tissues from 148 ESCC patients(P<0.001).Then,the Kaplan-Meier survival analysis suggested that ESCC patients with high HDAC7,c-Myc or USP10levels predicted worse overall survival(log-rank P<0.001).Co-IP and Western blotting further revealed that HDAC7physically deacetylated and activated β-catenin thus promoting downstream target c-Myc gene transcription.Notably,our mechanistic study validated that HDAC7/β-catenin/c-Myc could form the positive feedback loop to enhance ESCC cell growth,and USP10 could deubiquitinate and stabilize HDAC7 protein in the ESCC cells.Additionally,we verified that inhibition of the HDAC7/β-catenin/c-Myc axis and USP10/HDAC

关键词： melatonin Histone deacetylase 7(HDAC7) β-catenin c-Myc Ubiquitin-specifc peptidase 10(USP10) Esophageal squamous cell carcinoma(ESCC)

melatonin improves the first cleavage of parthenogenetic embryos from vitrified-warmed mouse oocytes potentially by promoting cell cycle progression

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2022年第1期13卷 106-122页

作者： Bo Pan Izhar Hyder Qazi Shichao Guo Jingyu Yang Jianpeng Qin Tianyi Lv Shengqin Zang Yan Zhang Changjun Zeng Qingyong Meng Hongbing Han Guangbin Zhou Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province College of Animal Science and TechnologySichuan Agricultural UniversityChengdu 611130China Department of Veterinary Anatomy and Histology Shaheed Benazir Bhutto University of Veterinary and Animal SciencesSakrandSindh 67210Pakistan State Key Laboratory of AgroBiotechnology China Agricultural UniversityBeijing 100193China National Engineering Laboratory for Animal Breeding Key Laboratory of Animal Genetics and Breeding of the Ministry of AgricultureBeijing Key Laboratory for Animal Genetic ImprovementCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Background:This study investigated the effect of melatonin(MT)on cell cycle(G1/S/G2/M)of parthenogenetic zygotes developed from vitrified-warmed mouse metaphase II(MII)oocytes and elucidated the potential mechanism of MT action in the first cleavage of embryos.Results:After vitrification and warming,oocytes were parthenogenetically activated(PA)and in vitro cultured(IVC).Then the spindle morphology and chromosome segregation in oocytes,the maternal mRNA levels of genes including Miss,Doc1r,Setd2 and Ythdf2 in activated oocytes,pronuclear formation,the S phase duration in zygotes,mitochondrial function at G1 phase,reactive oxygen species(ROS)level at S phase,DNA damage at G2 phase,early apoptosis in 2-cell embryos,cleavage and blastocyst formation rates were evaluated.The results indicated that the vitrification/warming procedures led to following perturbations 1)spindle abnormalities and chromosome misalignment,alteration of maternal mRNAs and delay in pronucleus formation,2)decreased mitochondrial membrane potential(MMP)and lower adenosine triphosphate(ATP)levels,increased ROS production and DNA damage,G1/S and S/G2 phase transition delay,and delayed first cleavage,and 3)increased early apoptosis and lower levels of cleavage and blastocyst formation.Our results further revealed that such negative impacts of oocyte cryopreservation could be alleviated by supplementation of warming,recovery,PA and IVC media with 10^(−9) mol/L MT before the embryos moved into the 2-cell stage of development.Conclusions:MT might promote cell cycle progression via regulation of MMP,ATP,ROS and maternal mRNA levels,potentially increasing the first cleavage of parthenogenetic zygotes developed from vitrified-warmed mouse oocytes and their subsequent development.

关键词： Cell cycle Cleavage rate melatonin MetaphaseⅡoocyte Parthenogenetic activation Vitrification

melatonin,tunneling nanotubes,mesenchymal cells,and tissue regeneration

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2023年第4期18卷 760-762页

作者： Francesca Luchetti Silvia Carloni Maria G.Nasoni Russel J.Reiter Walter Balduini Department of Biomolecular Sciences University of Urbino Carlo BoUrbinoItaly Department of Cell Systems and Anatomy Long School of MedicineUT HealthSan AntonioTXUSA

Mesenchymal stem cells are multipotent stem cells that reside in many human tissues and organs.Mesenchymal stem cells are widely used in experimental and clinical regenerative medicine due to their capability to transdifferentiate into various lineages.However,when transplanted,they lose part of their multipotency and immunomodulatory properties,and most of them die after injection into the damaged tissue.In this review,we discuss the potential utility of melatonin in preserving mesenchymal stem cells’survival and function after transplantation.melatonin is a pleiotropic molecule regulating critical cell functions including apoptosis,endoplasmic reticulum stress,and autophagy.melatonin is also synthesized in the mitochondria where it reduces oxidative stress,the opening of the mitochondrial permeability transition pore and the downstream caspase activation,activates uncoupling proteins,and curtails the proinflammatory response.In addition,recent findings showed that melatonin also promotes the formation of tunneling nanotubes and the transfer of mitochondria between cells through the connecting tubules.As mitochondrial dysfunction is a primary cause of mesenchymal stem cells death and senescence and a critical issue for survival after transplantation,we propose that melatonin by favoring mitochondria functionality and their transfer through tunneling nanotubes from healthy to suffering cells could improve mesenchymal stem cellbased therapy in a large number of diseases for which basic and clinical trials are underway.

关键词： brain ischemia cell transplantation melatonin mesenchymal stem cell mitochondria mitochondrial transplantation regenerative therapy senescence tunneling nanotubes

melatonin:a multitasking indoleamine to modulate hippocampal neurogenesis

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2023年第3期18卷 503-505页

作者： Alejandro Romero JoséÁngel Morales-García Eva Ramos Department of Pharmacology and Toxicology Faculty of Veterinary MedicineComplutense University of Madrid Institute for Biomedical Research“A.Sols”(CSIC-UAM) Spanish Center for Networked Biomedical Research on Neurodegenerative Diseases(CIBERNED Department of Cellular Biology School of MedicineComplutense University of MadridMadridSpain

Neurodegeneration affects a large number of cell types including neurons,astrocytes or oligodendrocytes,and neural stem cells.Neural stem cells can generate new neuronal populations through proliferation,migration,and differentiation.This neurogenic potential may be a relevant factor to fight neurodegeneration and aging.In the last years,we can find growing evidence suggesting that melatonin may be a potential modulator of adult hippocampal neurogenesis.The lack of therapeutic strategies targeting neurogenesis led researchers to explore new molecules.Numerous preclinical studies with melatonin observed how melatonin can modulate and enhance molecular and signaling pathways involved in neurogenesis.We made a special focus on the connection between these modulation mechanisms and their implication in neurodegeneration,to summarize the current knowledge and highlight the therapeutic potential of melatonin.

关键词： adult hippocampal neurogenesis aging melatonin neural stem cell neurodegeneration neuroprotection signaling pathway therapeutic strategy

melatonin inhibits Gram-negative pathogens by targeting citrate synthase

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2022年第7期65卷 1430-1444页

作者： Fang He Yuan Liu Pan Li Xiaoyan Wu Yaoyao Xia Dong Zhang Nengzhang Li Yuanyi Peng Guoqiang Zhu Rüdiger Hardeland Russel JReiter Wenkai Ren College of Veterinary Medicine Southwest UniversityChongqing 400715China State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Laboratory of Lingnan Modern AgricultureGuangdong Provincial Key Laboratory of Animal Nutrition ControlNational Engineering Research Center for Breeding Swine IndustryCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642China Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China Jiangsu Co-innovation Center for Important Animal Infectious Diseases and ZoonosesCollege of Veterinary MedicineInstitute of Comparative MedicineYangzhou UniversityYangzhou 225009China Johann Friedrich Blumenbach Institute of Zoology and Anthropology University of GöttingenGöttingen 37073Germany Department of Cellular and Structural Biology University of Texas Health Science CenterSan AntonioTX 78229USA

Bacterial infections caused by Gram-negative pathogens represent a growing burden for public health worldwide.Despite the urgent need for new antibiotics that effectively fight against pathogenic bacteria,very few compounds are currently under development or approved in the clinical setting.Repurposing compounds for other uses offers a productive strategy for the development of new antibiotics.Here we report that the multifaceted melatonin effectively improves survival rates of mice and decreases bacterial loads in the lung during infection.Mechanistically,melatonin specifically inhibits the activity of citrate synthase of Gram-negative pathogens through directly binding to the R300,D363,and H265 sites,particularly for the notorious Pasteurella multocida.These findings highlight that usage of melatonin is a feasible and alternative therapy to tackle the increasing threat of Gram-negative pathogen infections via disrupting metabolic flux of bacteria.

关键词： citrate synthase citric acid Gram-negative pathogens melatonin P.multocida

melatonin attenuates radiation-induced cortical bone-derived stem cells injury and enhances bone repair in postradiation femoral defect model

在线全文

学校读者我要写书评

暂无评论

Military Medical Research 2022年第3期9卷 319-330页

作者： Wei Hu Jia-Wu Liang Song Liao Zhi-Dong Zhao Yu-Xing Wang Xiao-Fei Mao Si-Wei Hao Yi-Fan Wang Heng Zhu Bin Guo Medical School of Chinese People’s Liberation Army(PLA) Beijing100853China Department of Stomatology the First Medical CentreChinese PLA General HospitalBeijing100853China Beijing Key Laboratory for Radiobiology Beijing Institute of Radiation MedicineBeijing100840China

Background:The healing of bone defects can be challenging for clinicians to manage,especially after exposure to ionizing radiation.In this regard,radiation therapy and accidental exposure to gamma(o inhibit bone formation and increase the risk of fractures.Cortical bone-derivγ)-ray radiation have been shown ted stem cells(CBSCs)are reportedly essential for osteogenic lineages,bone maintenance and repair.This study aimed to investigate the effects of melatonin on postradiation CBSCs and bone defect healing.Methods:CBSCs were extracted from C57BL/6 mice and were identified by flow cytometry.Then CBSCs were subjected to 6 Gyγ-ray radiation followed by treatment with various concentrations of melatonin.The effects of exogenous melatonin on the self-renewal and osteogenic capacity of postradiation CBSCs in vitro were analyzed.The underlying mechanisms involved in genomic stability,apoptosis and oxidative stress-related signaling were further analyzed by Western blotting,flow cytometry and immunofluorescence assays.Moreover,postradiation femoral defect models were established and treated with Matrigel and melatonin.The effects of melatonin on postradiation bone healing in vivo were evaluated by micro-CT and pathological analysis.Results:The decrease in radiation-induced self-renewal and osteogenic capacity were partially reversed in postradiation CBSCs treated with melatonin(Pmelatonin maintained genomic stability,reduced postradiation CBSC apoptosis and intracellular oxidative stress,and enhanced expression of antioxidant-related enzymes(Pmelatonin by downregulating interleukin-6(IL-6)and tumor necrosis factor alpha(TNF-α)levels via the extracellular regulated kinase(ERK)/nuclear factor erythroid 2-related factor 2(NRF2)/heme oxygenase-1(HO-1)signaling pathway.melatonin was also found to exhibit antioxidant effects via NRF2 signaling.In vivo experiments demonstrated that the newly formed bone in the melatonin plus Matrigel group had higher trabe

关键词： melatonin Stem cells Ionizing radiation Bone regeneration

melatonin biosynthesis pathways in nature and its production in engineered microorganisms

在线全文

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2022年第1期7卷 544-553页

作者： Xiaotong Xie Dongqin Ding Danyang Bai Yaru Zhu Wei Sun Yumei Sun Dawei Zhang Dalian Polytechnic University Dalian116000PR China Tianjin Institutes of Industrial Biotechnology Chinese Academy of SciencesTianjin300308PR China Biodesign Center Tianjin Institute of Industrial BiotechnologyChinese Academy of SciencesTianjin300308PR China Tianjin University of science and technology Tianjin300308PR China

melatonin is a biogenic amine that can be found in plants,animals and microorganism.The metabolic pathway of melatonin is different in various organisms,and biosynthetic endogenous melatonin acts as a molecular signal and antioxidant protection against external stress.Microbial synthesis pathways of melatonin are similar to those of animals but different from those of plants.At present,the method of using microorganism fermentation to produce melatonin is gradually prevailing,and exploring the biosynthetic pathway of melatonin to modify microorganism is becoming the mainstream,which has more advantages than traditional chemical synthesis.Here,we review recent advances in the synthesis,optimization of melatonin pathway.L-tryptophan is one of the two crucial precursors for the synthesis of melatonin,which can be produced through a four-step reaction.Enzymes involved in melatonin synthesis have low specificity and catalytic efficiency.Site-directed mutation,directed evolution or promotion of cofactor synthesis can enhance enzyme activity and increase the metabolic flow to promote microbial melatonin production.On the whole,the status and bottleneck of melatonin biosynthesis can be improved to a higher level,providing an effective reference for future microbial modification.

关键词： melatonin Biosynthesis Plants Animals Microorganisms Metabolic modification Enzyme catalysis mechanism