Selection of High-quality Sperms by the Nanotechnological Method of Magnetic Activation in Brazilian Cervids

作     者：Teresinha Inês Assumpção AndréLuíz Quagliatto Santos JoséMaurício Barbanti Duarte Maurício Foschini Caroline Silva Vieira 

作者机构：Teaching and Research Laboratory in Wild AnimalsFaculty of Veterinary Medicine and Animal ScienceFederal University of UberlandiaUberlandiaMGBrazil Deer Research and Conservation CentreDepartment of Animal ScienceSao Paulo State UniversityJaboticabalSPBrazil Physics InstituteFederal University of UberlandiaUberlandiaMGBrazil 

出 版 物：《Journal of Zoological Research》 (动物研究（英文）)

年 卷 期：2021年第3卷第2期

页      面：31-37页

学科分类：08[工学] 080502[工学-材料学] 0805[工学-材料科学与工程（可授工学、理学学位）] 

主　　题：Reproduction Andrology Fertility Deer Ejaculate Sperm cell 

摘      要：Cervids show a high degree of abnormalities in their sperm ***,this study aimed to select high-quality spermatozoa using magnetic-activated sperm sorting(MASS)compared to density gradient centrifugation(DGC)by assessing the post-selection cell *** from six Mazama deer was collected by electroejaculation after chemical *** semen was analyzed in four samples:Fresh,DGC,SEMgood-non-apoptotic fraction,and SEMpoor-apoptotic *** material was analyzed for motility and vigor(light microscopy),concentration(Neubauer chamber),semen morphology(phase contrast),and supravital staining test(eosin/nigrosine).The DGC method used 20 x 106 cells in 90%and 45%percoll®*** MASS used 10 x 106 cells with 20μl of iron nanoparticles attached to Annexin V and filtration in a magnetic separation *** processing methods(DGC and MASS)were effective in producing high-quality sperm samples,with a marked reduction in abnormalities from 41.83±10.25(fresh)to 14.83±3.17(DGC)and 12±3.01(SEMgood),with 80.3%±2.06 livings *** findings suggest that this nanotechnological method,using nanoparticles,effectively produces high-quality semen samples in cervids for use in assisted reproduction.