A 3D-printed magnetic digital microfluidic diagnostic platform for rapid colorimetric sensing of carbapenemase-producing Enterobacteriaceae

作     者：Pojchanun Kanitthamniyom Pei Yun Hon Aiwu Zhou Mohammad Yazid Abdad Zhi Yun Leow Nurhidayah Binte Mohamed Yazid Vanessa Lim Wei Xun Shawn Vasoo Yi Zhang 

作者机构：Singapore Centre for 3D PrintingSchool of Mechanical and Aerospace EngineeringNanyang Technological UniversitySingapore CitySingapore National Center for Infectious DiseaseTan Tock Seng HospitalSingapore CitySingapore Lee Kong Chian School of MedicineNanyang Technological UniversitySingapore CitySingapore 

出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)

年 卷 期：2021年第7卷第3期

页      面：173-184页

核心收录：

学科分类：100208[医学-临床检验诊断学] 0808[工学-电气工程] 1002[医学-临床医学] 0809[工学-电子科学与技术（可授工学、理学学位）] 0817[工学-化学工程与技术] 0807[工学-动力工程及工程热物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0802[工学-机械工程] 0811[工学-控制科学与工程] 0702[理学-物理学] 10[医学] 

基　　金：Yi Zhang and Shawn Vasoo are grateful for the funding support from NTUitive Gap Fund NGF-2020-08-002 Yi Zhang is thankful for funding support from the Singapore Ministry of Education Grant Tier 1 RG39/19 and the Nanyang Technological University Startup Grant 

主　　题：thereby operations consuming 

摘      要：Carbapenemase-producing Enterobacteriaceae(CPE)are a group of drug-resistant Gram-negative pathogens that are classified as a critical threat by the World Health Organization(WHO).Conventional methods of detecting antibiotic-resistant pathogens do not assess the resistance mechanism and are often time-consuming and *** have developed a magnetic digital microfluidic(MDM)platform,known as MDM Carba,for the identification of CPE by measuring their ability to hydrolyze carbapenem *** Carba offers the ability to rapidly test CPE and reduce the amount of reagents used compared with conventional phenotypic *** the MDM Carba platform,tests are performed in droplets that function as reaction chambers,and fluidic operations are accomplished by manipulating these droplets with magnetic *** simple droplet-based magnetic fluidic operation allows easy system automation and simplified hands-on *** of the unique“power-freeoperation of MDM technology,the MDM Carba platform can also be operated manually,showing great potential for point-of-care testing in resource-limited *** tested 27 bacterial isolates on the MDM Carba platform,and the results showed sensitivity and specificity that were comparable to those of the widely used Carba NP *** Carba may shorten the overall turnaround time for CPE identification,thereby enabling more timely clinical decisions for better clinical *** Carba is a technological platform that can be further developed to improve diagnostics for other types of antibiotic resistance with minor modifications.