Divergence of three BRX homoeologs in Brassica rapa and its effect on leaf morphology

作     者：Yuanyuan Zhang Jianli Liang Xu Cai Haixu Chen Jian Wu Runmao Lin Feng Cheng Xiaowu Wang Yuanyuan Zhang;Jianli Liang;Xu Cai;Haixu Chen;Jian Wu;Runmao Lin;Feng Cheng;Xiaowu Wang

作者机构：Institute of Vegetables and FlowersChinese Academy of Agricultural ScienceBeijingChina 

出 版 物：《Horticulture Research》 (园艺研究（英文）)

年 卷 期：2021年第8卷第1期

页      面：954-966页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：the National Natural Science Foundation of China(NSFC Grants 31630068 and 32072594) the National Program on Key Research Project(2016YFD01001007) the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences 

主　　题：expression. traits heading 

摘      要：The leafy head characteristic is a special phenotype of Chinese cabbage resulting from artificial selection during domestication and *** RADIX(BRX)has been suggested to control root elongation,shoot growth,and tiller angle in Arabidopsis and *** Brassica rapa,three BrBRX homoeologs have been identified,but only BrBRX.1 and BrBRX.2 were found to be under selection in leaf-heading accessions,indicating their functional diversification in leafy head ***,we show that these three BrBRX genes belong to a plant-specific BRX gene family but that they have significantly diverged from other BRX-like members on the basis of different phylogenetic classifications,motif compositions and expression ***,although the expression of these three BrBRX genes differed,compared with BrBRX.3,BrBRX.1,and BrBRX.2 displayed similar expression *** mutant complementation studies showed that only BrBRX.1 could rescue the brx root phenotype,whereas BrBRX.2 and BrBRX.3 could ***,overexpression of each of the three BrBRX genes in Arabidopsis resulted in similar pleiotropic leaf phenotypes,including epinastic leaf morphology,with an increase in leaf number and leaf petiole length and a reduction in leaf *** leaf traits are associated with leafy head *** testing of a SNP(T/C)in BrBRX.2 confirmed that this allele in the heading accessions was strongly associated with the leaf-heading trait of *** results revealed that all three BrBRX genes may be involved in the leaf-heading trait,but they may have functionally diverged on the basis of their differential expression.