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Cellular & Molecular Immunology 2024年第4期21卷 409-411页

作者： Dieter Kabelitz Institute of Immunology University of KielKielGermany

Members of the butyrophilin(BTN)family of transmembrane molecules play an important role in the activation of humanγδT cells.In recent years,it has been discovered that BTN3A1 and BTN2A1 are indispensable in this process,but other members like BTN3A2 and BTN3A3 are also required.So far,very little is known about the transcriptional and post-translational regulation of BTN expression.

关键词： expression. insight critical

VqMAPK3/VqMAPK6, VqWRKY33, and VqNSTS3 constitute a regulatory node in enhancing resistance to powdery mildew in grapevine

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Horticulture Research 2023年第7期10卷 293-308页

作者： Wandi Liu Chaohui Yan Ruimin Li Guanyu Chen Xinqi Wang Yingqiang Wen Chaohong Zhang Xiping Wang Yan Xu Yuejin Wang College of Horticulture Northwest A&F UniversityYanglingShaanxi712100China Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China Ministry of AgricultureYanglingShaanxi712100China State Key Laboratory of Crop Stress Biology in Arid Areas Northwest A&F UniversityYanglingShaanxi712100China

Grapevine powdery mildew is caused by Erysiphe necator,which seriously harms grape production in the world.Stilbene synthase makes phytoalexins that contribute to the resistance of grapevine against powdery mildew.A novel VqNSTS3 was identified and cloned from Chinese wild Vitis quinquangularis accession Danfeng-2.The novel VqNSTS3 was transferred into susceptible‘Thompson Seedless’by Agrobacterium-mediated transformation.The transgenic plants showed resistance to the disease and activated other resistance-related genes.VqNSTS3 expression.in grapevine is regulated by VqWRKY33,and which binds to TTGACC in the VqNSTS3 promoter.Furthermore,VqWRKY33 was phosphorylated by VqMAPK3/VqMAPK6 and thus led to enhanced signal transduction and increased VqNSTS3 expression.ProVqNSTS3::VqNSTS3-GFP of transgenic VqNSTS3 in Arabidopsis thaliana was observed to move to and wrap the pathogen’s haustoria and block invasion by Golovinomyces cichoracearum.These results demonstrate that stilbene accumulation of novel VqNSTS3 of the Chinese wild Vitis quinquangularis accession Danfeng-2 prevented pathogen invasion and enhanced resistance to powdery mildew.Therefore,VqNSTS3 can be used in generating powdery mildew-resistant grapevines.

关键词： invasion expression. resistance

Skp2 is a novel regulator of LSD1 expression.and function in gastric cancer

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Genes & Diseases 2023年第6期10卷 2267-2269页

作者： Moges Dessale Asmamaw Li-Rong Zhang Hong-Min Liu Xiao-Jing Shi Ying Liu Department of Pharmacology School of Basic Medical SciencesState Key Laboratory for Esophageal Cancer Prevention and TreatmentZhengzhou UniversityZhengzhouHenan 450001China School of Pharmaceutical Sciences Zhengzhou UniversityZhengzhouHenan 450001China Key Laboratory of Advanced Drug Preparation Technologies Ministry of Education of ChinaZhengzhouHenan 450001China Academy of Medical Sciences Zhengzhou UniversityZhengzhouHenan 450052China Department of Pharmacy The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan 450052China Henan Engineering Research Center for Application&Translation of Precision Clinical Pharmacy ZhengzhouHenan 450052China

S-phase kinase-associated protein 2(Skp2)is a well-characterized oncoprotein localized mainly in the nucleus and cytoplasm.It is an integral component of SCFskp2 E3 ubiquitin ligase complex which confers substrate selectivity to the ligase by specifically targeting a distinct set of proteins destined for proteasomal degradation such as p21,p27,cyclin E,and c-Myc.1 Skp2 is crucial in a multitude of cellular processes including cell cycle,cell proliferation,apoptosis,differentiation,and survival.However,despite its immense and well-established role in ubiquitin-proteasome system-mediated protein turnover,much is unknown about the function of Skp2 independent of the ubiquitination pathway.Previously,Skp2 has been reported to regulate RhoA gene transcription and the p300 signaling pathway in an E3 ligase-independent manner.2Moreover,Skp2 also acts as a cofactor for c-Myc-regulated gene expression.

关键词： Skp2 expression. function

简短,但重要:短串联重复序列赋予转录因子与DNA结合的可变性

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Science Bulletin 2024年第1期69卷 9-10页

作者：张晶朱冰 National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Key Laboratory of Epigenetic Regulation and Intervention Chinese Academy of SciencesBeijing 100101China New Cornerstone Science Laboratory Institute of BiophysicsChinese Academy of SciencesBeijing 100101China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China

Sequence-specific transcription factors(TFs)selectively bind to the regulatory regions of target genes to regulate gene expression.Although an old topic,how TFs specifically and efficiently identify and bind to their target sequences is not fully understood.The dominant idea holds that TFs bind short sequence-specific DNA motifs within regulatory regions via direct base interactions andDNA shape recognition[1].

关键词： expression. 短串联重复序列 holds

Defining two subpopulations of marginal zone B cells

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Cellular & Molecular Immunology 2024年第4期21卷 412-413页

作者： Xiaojing Liu Fei-Long Meng Key Laboratory of RNA Science and Engineering Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai200031China

Marginal zone(MZ)B cells,which are composed of heterogeneous subpopulations,are participate in the rapid response to antigens.Lee et al.showed that MZ B cells can be divided into two distinct subpopulations based on CD80 expression.These two subpopulations of MZ B cells exhibit differential autoreactivity,radiosensitivity,and functional capacities.

关键词： expression. subpopulations al.

expression.and possible role of Smad3 in postnecrotizing enterocolitis stricture

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World Journal of Pediatric Surgery 2022年第1期5卷 30-39页

作者： Rui Chen Chengjie Lv Xiaoxia Zhao Dong Ma Dengming Lai Yun Zhao Luyin Zhang Jinfa Tou Department of Neonatal Surgery Zhejiang University School of Medicine Children's HospitalHangzhouPeople's Republic of China National Center for clinical medicine of children's health and disease HangzhouPeople's Republic of China Department of Pathology Zhejiang University School of Medicine Children's HospitalHangzhouPeople's Republic of China Pediatric Surgery Affiliated Hangzhou First People's HospitalZhejiang University School of MedicineHangzhouPeople's Republic of China

Objective To investigate the expression.of Smad3(mothers against decapentaplegic homolog 3)protein in postnecrotizing enterocolitis stricture and its possible mechanism of action.Methods We used immunohistochemistry to detect the expression.characteristics of Smad3 and nuclear factor kappa B(NF-κB)proteins in human postnecrotizing enterocolitis stricture.We cultured IEC-6(crypt epithelial cells of rat small intestine)in vitro and inhibited the expression.of Smad3 using siRNA technique.Quantitative PCR,western blotting,and ELISA were used to detect the changes in transforming growth factor-β1(TGF-β1),NF-κB,tumor necrosis factor-α(TNF-α),vascular endothelial growth factor(VEGF),and zonula occludens-1(ZO-1)messenger RNA(mRNA)and protein expression. in IEC-6 cells.CCK8 kit and Transwell cellular migration were used to detect cell proliferation and migration.Changes in epithelial-mesenchymal transition(EMT)markers(E-cadherin and vimentin)in IEC-6 cells were detected by immunofluorescence technique.Results The results showed that Smad3 protein and NF-κB protein were overexpressed in narrow intestinal tissues and that Smad3 protein expression.was positively correlated with NF-κB protein expression.After inhibiting the expression.of Smad3 in IEC-6 cells,the mRNA expression. of NF-κB,TGF-β1,ZO-1,and VEGF decreased,whereas the mRNA expression.of TNF-αdid not significantly change.TGF-β1,NF-κB,and TNF-αprotein expression. in IEC-6 cells decreased,whereas ZO-1 and intracellular VEGF protein expression. increased.IEC-6 cell proliferation and migration capacity decreased.There was no significant change in protein expression.levels of EMT markers E-cadherin and vimentin and also extracellular VEGF protein expression.Conclusions We suspect that the high expression.of Smad3 protein in postnecrotizing enterocolitis stricture may promote the occurrence and development of secondary intestinal stenosis.The mechanism may be related to the regulation of TGF-β1,NF-κB,TNF-α,ZO-1,and VEGF mRNA and protein expression.This may also be relate

关键词： Smad3 expression. stricture

Alternative splicing and cancer: a systematic review

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Signal Transduction and Targeted Therapy 2021年第3期6卷 807-820页

作者： Yuanjiao Zhang Jinjun Qian Chunyan Gu Ye Yang The Third Affiliated Hospital of Nanjing University of Chinese Medicine NanjingChina School of Medicine&Holistic Integrative Medicine Nanjing University of Chinese MedicineNanjingChina

The abnormal regulation of alternative splicing is usually accompanied by the occurrence and development of tumors,which would produce multiple different isoforms and diversify protein expression.The aim of the present study was to conduct a systematic review in order to describe the regulatory mechanisms of alternative splicing,as well as its functions in tumor cells,from proliferation and apoptosis to invasion and metastasis,and from angiogenesis to metabolism.The abnormal splicing events contributed to tumor progression as oncogenic drivers and/or bystander factors.The alterations in splicing factors detected in tumors and other mis-splicing events(i.e.,long non-coding and circular RNAs)in tumorigenesis were also included.The findings of recent therapeutic approaches targeting splicing catalysis and splicing regulatory proteins to modulate pathogenically spliced events(including tumor-specific neo-antigens for cancer immunotherapy)were introduced.The emerging RNA-based strategies for the treatment of cancer with abnormally alternative splicing isoforms were also discussed.However,further studies are still required to address the association between alternative splicing and cancer in more detail.

关键词： expression. invasion alterations

Three bona fide plant-specific SAGA subunits and their regulatory function

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Molecular Plant 2021年第7期14卷 1033-1035页

作者： J.S.Ramirez-Prado M.Benhamed Centre of Microbial and Plant Genetics KU Leuven3001 LeuvenBelgium lnstitute of Plant Sciences Paris-Saclay(IPS2) Universite Paris-SaclayCNRSINRAEUniv EvryOrsay 91405France Institute of Plant Sciences Paris-Saclay Universite de ParisCNRSINRAEOrsay(IPS2)91405France lnstitut Universitaire de France(IUF) ParisFrance

Nucleosomal histone tails are subject to a vast repertoire of covalent modifications that alter the physical accessibility of their associated DNA,impacting gene expression.Specifically,the acetylation of histones is associated with transcriptional activation,as it has been shown to reduce their affinity for DNA(Grant,2001).The dynamic changes on histone acetylation levels throughout development are the result of active acetylation and deacetylation processes,mediated by histone acetyltransferases(HATs)and deacetylases,respectively.

关键词： expression. SAGA transcriptional

APOLO lncRNA, a self-calibrating switch of root development

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Molecular Plant 2021年第6期14卷 867-869页

作者： Tibor Csorba GBI MATE UniversityGödöllőHungary

Long non-protein coding RNAs(lncRNAs)are emerging as important regulators of gene expression.In a series of studies,Ariel and co-workers revealed the molecular mechanisms of AUXIN REGULATED PROMOTER LOOP RNA(APOLO)lncRNA actions(Ariel et al.,2014,2020;Moison et al.,2021).APOLO integrates endogenous and exogenous signals and,in combination with an intricate network of epigenetic and transcriptional factors,controls multiple aspects of root development.Notably,APOLO acts as both positive and negative regulator to orchestrate gene expression.in cis and in trans.

关键词： expression. al. endogenous

Large-scale identification of expression.quantitative trait loci in Arabidopsis reveals novel candidate regulators of immune responses and other processes

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Journal of Integrative Plant Biology 2020年第10期62卷 1469-1483页

作者： Xingang Wang Min Ren Danni Liu Dabao Zhang Cuijun Zhang Zhaobo Lang Alberto PMacho Min Zhang Jian-Kang Zhu Department of Horticulture and Landscape Architecture Purdue UniversityWest LafayetteIndiana47907USA Cold Spring Harbor Laboratory Cold Spring HarborNew York11724USA Department of Statistics Purdue UniversityWest LafayetteIndiana47907USA Shanghai Center for Plant Stress Biology Center of Excellence for Molecular Plant SciencesShanghai 200032China

The extensive phenotypic diversity within natural populations of Arabidopsis is associated with differences in gene expression.Transcript levels can be considered as in-heritable quantitative traits,and used to map expression.quantitative trait loci(eQTL)in genome-wide association studies(GWASs).In order to identify putative genetic de-terminants for variations in gene expression.we used pub-licly available genomic and transcript variation data from 665 Arabidopsis accessions and applied the single nucleotide polymorphism-set(Sequence)Kernel Association Test(SKAT)method for the identification of eQTL.Moreover,we used the penalized orthogonal-components regression(POCRE)method to increase the power of statistical tests.Then,gene annotations were used as test units to identify genes that are associated with natural variations in transcript accumulation,which correspond to candidate regulators,some of which may have a broad impact on gene ex-pression.Besides increasing the chances to identify real as-sociations,the analysis using POCRE and SKAT significantly reduced the computational cost required to analyze large datasets.As a proof of concept,we used this approach to identify eQTL that represent novel candidate regulators of immune responses.The versatility of this approach allows its application to any process that is subjected to natural var-iation among Arabidopsis accessions.

关键词： expression. regulators traits