Simultaneous harvesting and cell disruption of microalgae using ozone bubbles:optimization and characterization study for biodiesel production

作     者：Wan N.A.Kadir Man K.Lam Yoshimitsu Uemura Jun W.Lim Peck L.Kiew Steven Lim Siti S.Rosli Chung Y.Wong Pau L.Show Keat T.Lee 

作者机构：Chemical Engineering DepartmentUniversiti Teknologi PETRONASSeri Iskandar32610Malaysia HICoE-Centre for Biofuel and Biochemical ResearchInstitute of Self-Sustainable BuildingUniversiti Teknologi PETRONASSeri Iskandar32610Malaysia NPO Kuramae BioenergyTokyo108-0023Japan Fundamental and Applied Sciences DepartmentUniversiti Teknologi PETRONASSeri Iskandar32610Malaysia Faculty of EngineeringTechnology&Built EnvironmentUniversity College Sedaya InternationalCheras Kuala Lumpur56000Malaysia Department of Chemical EngineeringLee Kong Chian Faculty of Engineering and ScienceUniversiti Tunku Abdul RahmanSungai LongSelangor43000Malaysia Department of Chemical and Environmental EngineeringFaculty of Science and EngineeringUniversity of Nottingham MalaysiaSemenyih43500Malaysia School of Chemical EngineeringUniversiti Sains MalaysiaEngineering CampusNibong Tebal14300Malaysia 

出 版 物：《Frontiers of Chemical Science and Engineering》 (化学科学与工程前沿（英文版）)

年 卷 期：2021年第15卷第5期

页      面：1257-1268页

核心收录：

学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 

基　　金：the financial support provided by the Ministry of Higher Education(MOHE)Malaysia through Fundamental Research Grant Scheme(FRGS)with cost center Grant No.0153AB-L25 Fundamental Research Grant Scheme Malaysia’s Rising Star Awards 2016(FRGS MRSA 2016)with cost center Grant No.203/PJKIMIA/6071362 Support from MOHE through HICoE award to CBBR is duly acknowledged(cost centre Grant No.015MA0-052) 

主　　题：microalgae biodiesel ozonation pre-treatment lipid 

摘      要：In the present study, ozone was introduced as an alternative approach to harvest and disrupt microalgae cells (Chlorella vulgaris) simultaneously for biodiesel production. At the optimum ozonation conditions (6.14 g·h^(−1) ozone concentration, 30 min ozonation time, 1 L·min^(−1) of ozone flowrate at medium pH of 10 and temperature of 30℃), the sedimentation efficiency of microalgae cells increased significantly from 12.56% to 68.62%. It was observed that the microalgae cells aggregated to form flocs after pretreated with ozone due to the increment of surface charge from −20 to −6.59 mV. Besides, ozone had successfully disrupted the microalgae cells and resulted in efficient lipid extraction, which was 1.9 times higher than the control sample. The extracted microalgae lipid was mainly consisted of methyl palmitate (C16:0), methyl oleate (C18:1) and methyl linolenate (C18:3), making it suitable for biodiesel production. Finally, utilization of recycled culture media after ozonation pre-treatment showed robust growth of microalgae, in which the biomass yield was maintained in the range of 0.796 to 0.879 g ·h^(−1) for 5 cycles of cultivation.