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Construction of algal-bacterial consortia using green microalgae Chlorella vulgaris and As(Ⅲ)-oxidizing bacteria:As tolerance and metabolomic profiling

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Journal of Environmental Sciences 2024年第5期139卷 258-266页

作者： Xiaoman He Guobing Lin Jiayuan Zeng Zhaoguang Yang Lin Wang College of Chemistry and Chemical Engineering Central South UniversityChangsha 410083China Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety Central South UniversityChangsha 410083China

Bioremediation became a promising technology to resolve arsenic(As)contamination in aquatic environment.Since monoculture such as microalgae or bacteria was sensitive to environmental disturbance and vulnerable to contamination,green microalgae Chlorella vulgaris and arsenite(As(Ⅲ))-oxidizing bacteria Pseudomonas sp.SMS11 were co-cultured to construct algal-bacterial consortia in the current study.The effects of algae-bacteria(A:B)ratio and exposure As(Ⅲ)concentration on algal growth,As speciation and metabolomic profile were investigated.Algal growth arrested when treated with 100 mg/L As(Ⅲ)without the co-cultured bacteria.By contrast,co-cultured with strain SMS11 significantly enhanced As tolerance in C.vulgaris especially with A:B ratio of 1:10.All the As(Ⅲ)in culture media of the consortia were oxidized into As(Ⅴ)on day 7.Methylation of As was observed on day 14.Over 1% and 0.5% of total As were converted into dimethylarsinic acid(DMA)after 21days cultivation when the initial concentrations of As(Ⅲ)were 1 and 10 mg/L,respectively.Metabolomic analysis was further performed to reveal the response of consortia metabolites to external As(Ⅲ).The enriched metabolomic pathways were associated with carbohydrate,amino acid and energy metabolisms.Tricarboxylic acid cycle and glyoxylate and dicarboxylate metabolism were upregulated under As stress due to their biological functions on alleviating oxidative stress and protecting cells.Both carbohydrate and amino acid metabolisms provided precursors and potential substrates for energy production and cell protection under abiotic stress.Alterations of the pathways relevant to carbohydrate or amino acid metabolism were triggered by energy requirement.

关键词： microalgae Algal-bacterial consortia As(Ⅲ)-oxidizing bacteria Metabolomics Energy metabolism

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Heavy metal pollution of river water and eco-friendly remediation using potent microalgal species

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Water Science and Engineering 2024年第1期17卷 41-50页

作者： Amudham Radha Amal Raj Prabhakaran Mylsamy V.Sivasankar B.Sathish Kumar Kiyoshi Omine T.G.Sunitha Phycological Research Laboratory Research Department of BotanyPachaiyappa's College(Affliated to University of Madras)Chennai 600030India Research Department of Chemistry Pachaiyappa's College(Affliated to University of Madras)Chennai 600030India Department of Botany Sri Vidya Mandir Arts&Science College(Autonomous)KatteriUthangarai 636902India Department of Civil Engineering School of Engineering.Nagasaki UniversityNagasaki-Daigaku1-14 Bunkyo-machiNagasaki 8528521Japan

Pollution of rivers is mainly caused by anthropogenic activities such as discharge of effluent from industrial facilities,maintenance of sewage/effluent treatment plants,and dumping of solid waste on river banks.This study dealt with the pollution issues of the Cooum River in the well-known city of Chennai in South India.Water samples from 27 locations were collected and analyzed for 12 elements,including Ba,B,and Al,as well as heavy metals such as Pb,Cr,Mn,Fe,Co,Ni,Cu,Zn,and Cd.The samples showed levels of these elements that exceeded World Health Organization recommendations.Pearson correlation analysis revealed the inter-dependency among elements,and the contribution of each element based on factor loadings showed its percentage contribution compared to others.Water samples from six significant locations were chosen for remediation with three algae:Chlorella vulgaris,Scenedesmus dimorphus,and Phormedium sp.The uptake of pollutants led to the continuous growth of algae during the incubation period of 15 d,effectively removing heavy metals from the river water.The increasing levels of algal counts and the chlorophyll a content confirmed the algal growth during the incubation period,followed by a declining stage after the incubation period.The scanning electron microscopic images of algae before and after the remediation showed no remarkable modification of morphological patterns.This study showed that the uptake of heavy metals using algae is an effective water pollution remediation measure,making the process practicable in the field on a large scale in the near future.

关键词： Heavy metal Cooum river Phycoremediation microalgae Factor analysis

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Regulation of different light conditions for efficient biomass production and protein accumulation of Spirulina platensis

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Journal of Oceanology and Limnology 2024年第1期42卷 174-186页

作者： Yufei ZHANG Xianjun LI Yuhui LI Shiqi LIU Yanrui CHEN Miao JIA Xin WANG Lu ZHANG Qiping GAO Liang ZHANG Daoyong YU Baosheng GE State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology China University of Petroleum(East China)Qingdao 266580China Tongwei Co. Ltd.Healthy Aquaculture Key Laboratory of Sichuan ProvinceChengdu 610093China

Light plays an important role in the photosynthesis and metabolic process of microalgae.However,how different light conditions regulate the biomass production and protein accumulation of microalgae is mostly unknown.In this study,the influence of different light conditions,including light colors,densities,and light:dark cycles on the cell growth and biochemical composition of Spirulina platensis was symmetrically characterized.Under different colored lights,S.platensis all shows an increase trend within the increased light intensity ranges;however,each showing different optimal light intensities.At the same light intensity,different colored lights show different growth rate of S.platensis following the sequence of red>white>green>yellow>blue.The maximum growth rate and protein accumulation were determined as 21.88 and 5.10 mg/(L·d)when illuminated under red LED.The energy efficiency of different light sources was calculated and ranked as red>white>blue≈green>yellow.Transcriptomic analysis suggests that red light can promote cell growth and protein accumulation by upregulating genes related to photosynthesis,carbon fixation,and C-N metabolism pathways.This study provides a conducive and efficient way to promote biomass production and protein accumulation of S.platensis by regulating light conditions.

关键词： microalgae light emitting diode protein accumulation biomass production transcriptomic analysis

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Microalgal bioinoculants for sustainable agriculture and their interactions with soil biotic and abiotic components:A review

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Pedosphere 2024年第2期34卷 297-314页

作者： Shisy JOSE Nirmal RENUKA Sachitra Kumar RATHA Sheena KUMARI Faizal BUX Institute for Water and Wastewater Technology Durban University of TechnologyP.O.Box 1334Durban 4000South Africa Algal Biotechnology Laboratory Department of BotanyCentral University of PunjabBathinda 151401India Phycology Laboratory Council of Scientific and Industrial Research-National Botanical Research InstituteLucknowUttar Pradesh 226001India

Modern agricultural practices have posed a detrimental impact on the environment due to their intensive use to meet the food demands of an ever-increasing population.In this context,microalgal bioinoculants,specifically cyanobacteria and green microalgae,have emerged as sustainable options for agricultural practices to improve soil organic carbon,nutrient availability,microbial quality,and plant productivity.An overview of current and future perspectives on the use of microalgal bioinoculants in agriculture practices is presented in this review,along with a discussion of their interactions with soil biotic and abiotic factors that affect soil fertility,plant health,and crop productivity.The benefits of microalgal bioinoculants include releasing agronomically important metabolites(exopolymers and phytohormones)as well as solubilizing soil nutrients.Furthermore,they function as biocontrol agents against soil-borne pathogens and facilitate the establishment of rhizosphere communities of agricultural importance.So far,very few studies have explored the basic mechanisms by which microalgal bioinoculants interact with soil biotic and abiotic factors.In recent years,advanced molecular techniques have contributed to a better understanding of these interactions.

关键词： biocontrol agent biofertilizer cyanobacteria microalgae microbial community molecular technique plant growth soil fertility

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Biomanufacturing ofγ-linolenic acid-enriched galactosyldiacylglycerols:Challenges in microalgae and potential in oleaginous yeasts

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Synthetic and Systems Biotechnology 2023年第3期8卷 469-478页

作者： Xiaosong Gu Lei Huang Jiazhang Lian Key Laboratory of Biomass Chemical Engineering of Ministry of Education College of Chemical and Biological EngineeringZhejiang UniversityHangzhou310027China ZJU-Hangzhou Global Scientific and Technological Innovation Center Zhejiang UniversityHangzhou311215China Zhejiang Key Laboratory of Smart Biomaterials Zhejiang UniversityHangzhou310027China

γ-Linolenic acid-enriched galactosyldiacylglycerols(GDGs-GLA),as the natural form ofγ-linolenic acid in microalgae,have a range of functional activities,including anti-inflammatory,antioxidant,and anti-allergic properties.The low abundance of microalgae and the structural stereoselectivity complexity impede microalgae extraction or chemical synthesis,resulting in a lack of supply of GDGs-GLA with a growing demand.At present,there is a growing interest in engineering oleaginous yeasts for mass production of GDGs-GLA based on their ability to utilize a variety of hydrophobic substrates and a high metabolic flux toward fatty acid and lipid(triacylglycerol,TAG)production.Here,we first introduce the GDGs-GLA biosynthetic pathway in microalgae and challenges in the engineering of the native host.Subsequently,we describe in detail the applications of oleaginous yeasts with Yarrowia lipolytica as the representative for GDGs-GLA biosynthesis,including the development of synthetic biology parts,gene editing tools,and metabolic engineering of lipid biosynthesis.Finally,we discuss the development trend of GDGs-GLA biosynthesis in Y.lipolytica.

关键词： γ-Linolenic acid-enriched galactosyldiacylglycerols(GDGs-GLA) microalgae Yarrowia lipolytica Synthetic biology Metabolic engineering

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Anaerobic co-digestion of thermo-alkaline pretreated microalgae and sewage sludge: Methane potential and microbial community

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Journal of Environmental Sciences 2023年第5期127卷 133-142页

作者： Jiaqi Fu Bing Yan Shuanglin Gui Yinxuan Fu Song Xia Institute of Energy Research Jiangxi Academy of SciencesNanchang 330096China

To improve methane production from sewage sludge(SS),co-digestion of SS and microalgae(MA)was studied and the application of thermo-alkaline pretreatment to MA was evaluated.The results showed that thermo-alkaline pretreatment at 90℃ for 120 min on MA was the optimum pretreatment condition.Furthermore,when the volatile solids(VS)ratio of SS and MA was 1:2,the methane yield reached maximum(368.94 mL/g VS).Fourier transform infrared(FT-IR)and thermogravimetric analysis confirmed the synergetic effects of thermoalkaline pretreated MA on its co-digestion with SS.The analyses of microbial community indicated that Methanobacterium and Methanosarcina were the dominant methanogens during the co-digestion process.However,the relative abundance of Methanosarcina in thermoalkaline pretreated groups was higher compared to unpretreated groups.The microbial community structure might be affected by thermo-alkaline pretreatment rather than by the MA dosage in the co-digestion.

关键词： Anaerobic co-digestion Thermo-alkaline pretreatment microalgae Sewage sludge Microbial community

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A sustainable integration of removing CO_(2)/NO_(x) and producing biomass with high content of lipid/protein by microalgae

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Journal of Energy Chemistry 2022年第10期31卷 13-25,I0002页

作者： Junying Zhu Baowen Guo Fengxiang Qie Xu Li Xikang Zhao Junfeng Rong Baoning Zong Research Centre of Renewable Energy Research Institute of Petroleum ProcessingSINOPECBeijing 100083China

Due to the boost of CO_(2)/NO_(x)emissions which cause environmental pollution,processes that remove such pollutants from flue gas have attracted increasing attention in recent years.Among these technologies,biological CO_(2)/NO_(x)emission reduction has received more interest.microalgae,a kind of photosynthetic microorganism,offer great promise to convert CO_(2)/NO_(x)to biomass with high content of lipid and protein,which can be used as feedstock for various products such as biodiesel,health products,feedstuff and biomaterials.In this paper,biological CO_(2)/NO_(x)removing technologies by microalgae,together with the products(such as biofuel and protein)and their economic viability are discussed.Although commercial applications of microalgae for biodiesel and protein products are hampered by the high production cost of biomass,the use of CO_(2)/NO_(x)from flue gas as carbon and nitrogen sources can reduce the cost of biomass production,which makes these technologies more competent for real-life applications.Moreover,it is projected that the increasing in CO_(2)allowances will lead to further reduction in the cost of biomass production,which especially favors related products with lower values such as biodiesel.Furthermore,by combining various process optimization and integration,biorefinery is proposed and considered as the crucial component for the sustainable and economically feasible bulk applications of microalgae biomass.

关键词： microalgae Greenhouse gas NO_(x) Biodiesel Protein

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Diversity and community structure of eukaryotic microalgae in surface sediments in the central Bohai Sea,China,based on a metabarcoding approach

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Journal of Oceanology and Limnology 2022年第6期40卷 2277-2291页

作者： Zhaohui WANG Chaofan WANG Maoting WANG Weiguo LI Wencong ZHONG Lei LIU Tao JIANG College of Life Science and Technology Jinan UniversityGuangzhou 510632China Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of EducationGuangzhou 510632China School of Ocean Yantai UniversityYantai 264005China

Sediment samples were collected at 17 stations in the central Bohai Sea,China,and the diversity and community structure of eukaryotic microalgae were assessed by metabarcoding the V4 region of 18S r DNA.A total of 930 operational taxonomic units(OTUs)were detected for microeukaryotes,including 98algal OTUs.The algal communities comprised 42 genera belonging to 19 classes of six phyla,and they were dominated by chrysophytes and dinoflagellates.Dinoflagellates were also the most diverse microalgal group.The nano-sized dinoflagellates Biecheleria halophila and Azadinium trinitatum occurred abundantly in the study area;however,they have not been reported previously,as they may be overlooked or misidentified in light microscopy.Many pico-sized chlorophytes were detected in the sediment samples.Sixteen of the detected OTUs were assigned to potentially harmful and/or bloom-forming microalgae,suggesting some potential risks of harmful algal blooms in the central Bohai Sea.The capacity of metabarcoding to detect morphologically cryptic and small species makes this method a sufficiently sensitive means of detection for assessing eukaryotic microalgae in sediments.

关键词： microalgae dinoflagellate cysts high throughput sequencing marine sediment the Bohai Sea resting stages

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Post Cryopreservation Growth Kinetic and Photosynthetic Assessment of an Acid Tolerant Strain of Stichococcus bacillaris

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Natural Resources 2024年第5期15卷 130-148页

作者： Marzia Licata Luigi Marra Nunzia Nappi Elena Aurino Feliciana Oliva Antonino De Natale Donato Giovannelli Antonino Pollio Department of Biology University of Naples &#8220Federico II&#8221 Naples Italy ACUF Algal Collection of University of Naples &#8220Federico II&#8221 Naples Italy Department of Chemical Materials and Industrial Production Engineering University of Naples &#8220Federico II&#8221 P.le V. Tecchio Naples Italy Institute for Marine Biological Resources and Biotechnologies Italian National Research Council CNR-IRBIM Ancona Italy Earth-Life Science Institute ELSI Tokyo Institute of Technology Tokyo Japan Marine Chemistry and Geochemistry Department Woods Hole Oceanographic Institution Woods Hole MA USA Department of Marine and Coastal Science Rutgers University New Brunswick NJ USA

Preserving microbial diversity has become a strategic undertaking. Thus, ex situ microalgal culture conservation results in strategic and functional resource in both biodiversity protection and application domains. Cryopreservation of microalgae has been practiced since the 1960s and is now considered the optimal preservation strategy. Furthermore, the overall monitoring during growth of cultures after freezing/thawing protocols was hardly investigated and there is poor evaluation related to preserve especially the photosystem apparatus. The present study focuses on Stichococcus bacillaris as case study for short-term cryopreservation at −80 °C storage. Various freezing pretreatments using cryoprotective agents, and two thawing methods were compared introducing a novel variable to evaluate viability recovery and assessing growth kinetics of cultures immediately after thawing and after a series batch cultivation. Photosynthetic rate and pigments assessment were proposed to evaluate hidden metabolic cell damage. Results underline cryoprotective agents can increase the kinetic recovery of preserved cells in terms of reduction of lag phase during batch cultivation tests: the use of dimethyl sulfoxide and glycerol granted a growth comparable to unpreserved cells when sudden thawing occurs after 24 hours of storage, but recovery after preservation is less sensitive to cryoprotective agents when gradual thawing and 1 month of storage is considered. However, cells are always able to restore their physiological pathways even without agents, so their kinetic effect has been proved and quantified. Interestingly, both the photosynthetic efficiency and the ratio between total chlorophyll and carotenoids are comparable (0.75 Fv/Fm, 2.2 ± 0.25 g/g) to unpreserved cells and they are unsensitive to chosen agents, but the ratio between chlorophyll a and chlorophyll b was clearly altered (up to 10 times), suggesting that photoactive pigments relative proportions can result in similar growth kinetic performance

关键词： microalgae Stichococcus bacillaris Cryopreservation Growth Kinetic Photosynthetic Rate

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Fatty acids in microalgae and cyanobacteria in a changing world:Contrasting temperate and cold environments

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BIOCELL 2022年第3期46卷 607-621页

作者： MARCELO P.HERNANDO IRENE R.SCHLOSS FLORENCIA DE LA ROSA MARLEEN DE TROCH Comisión Nacional de Energía Atómica(CNEA) Centro Atómico ConstituyentesDepartamento de RadiobiologíaSan MartínBuenos AiresArgentina Red de Investigación de Estresores Marinos-costeros en América Latina y el Caribe(REMARCO) Mar del Plata7602Argentina Instituto Antártico Argentino(IAA) San MartínBuenos AiresArgentina Centro Austral de Investigaciones Científicas(CADIC-CONICET) Ushuaia9410Argentina Universidad Nacional de Tierra del Fuego Ushuaia9410Argentina Instituto de Ciencias Básicas y Experimentales(ICBE) Universidad de MorónMorónBuenos AiresArgentina Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET) Buenos AiresArgentina Ghent University Faculty of SciencesBiology DepartmentMarine BiologyGhent9000Belgium

Under the present changing climate conditions and the observed temperature increase,it is of high importance to understand its effects on aquatic microbial life,and organisms’adaptations at the biochemical level.To adjust to temperature or salinity stress and avoid cell damage,organisms alter their degree of fatty acids(FAs)saturation.Thus,temperature is expected to have strong effects on both the quantity and quality of FAs in aquatic microorganisms.Here we review some recent findings about FAs sensitivity to climate change in contrasting environments.Overall,heat waves may induce changes in the relative abundance of polyunsaturated FAs(PUFA).However,the impact of the exposure to warming waters is different in temperate and polar environments.In cold marine waters,high concentration of omega-3(ω3)FAs such as eicosapentaenoic acid(EPA)is promoted due to the activation of the desaturase enzyme.In this way,cells have enough energy to produce or activate antioxidant protection mechanisms and avoid oxidative stress due to heat waves.Contrastingly,under high irradiance and heat wave conditions in temperate environments,photosystems’protection is achieved by decreasing EPA concentration due to desaturase sensitivity.Essential FAs are transferred in aquatic food webs.Therefore,any alteration in the production of essential FAs by phytoplankton(the main source ofω3)due to climate warming can be transferred to higher trophic levels,with cascading effects for the entire aquatic ecosystem.

关键词： Antarctic Temperate Essential FAs Increased temperature microalgae Cyanobacteria

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