Human urine metabolome in response to Fermentum rubrum(Hongqu Capsules)by HILIC-UHPLC-QTOF

作     者：HongYANG EdmundJ.D.LEE SeokHweeKOO FengTIAN 

作者机构：School of Applied Science Temasek Polytechnic Singapore 529757 Department of Pharmacology Yong Loo Lin School of Medicine National University of Singapore Singapore 117597 Clinical Trials & Research Unit Changi General Hospital Singapore 529889 

出 版 物：《中国药理学与毒理学杂志》 (Chinese Journal of Pharmacology and Toxicology)

年 卷 期：2015年第29卷第S1期

页      面：106-106页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：The project supported by Temasek Polytechnic DRP project grant 

主　　题：urine metabolome Fermentum rubrum HILIC-UHPLC-QTOF 

摘      要：OBJECTIVE ″-omics″study represents an unbiased perspective to examine the bio-system to discover the novel biomarker(s)which might be overlooked when targeted analysis is performed ***,due to its ease of collection,minimal invasion involved and rich information of the downstream metabolites,has been extensively *** rubrum(Hongqu,HQ capsules)is a well-known traditional medicine with the claimed slimming effect which could be related to the reduction in the deposition of total cholesterol and *** in sufficient clinical evidence is always one of the leading reasons that hamper traditional medicines from gaining world-wide *** our pursuit of scientific support for HQ capsules in managing obesity,we aim to examine the alteration of urinary metabolites in this small-scale human clinical *** 6 Chinese subjects were included and exposed to short-term administration of HQ capsules for 3d,twice daily,two capsules each *** urine samples were collected for three days prior to the dosing and on the day following the last *** total,96 urine samples were collected and then separated on hydrophilic interaction chromatography(HILIC)and analyzed by Agilent 6550 ESI-Quadrupoletime-of-flight(QTOF)mass *** Under positive mode,two compounds were found to be present only in post-dosing urine,and one compound was significantly lowered in post-dosing *** two compounds might be associated with the administered HQ capsules,which is formulated with multiherbal *** Further elucidation on the structures of these compounds is needed to enable better understanding of the mechanism of HQ capsules in managing obesity.