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A Dual Mechanism Controls Nuclear Localization in the Atypical Basic-Helix-Loop-Helix Protein PAR1 of Arabidopsis thaliana

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Molecular Plant 2012年第3期5卷 669-677页

作者： Anahit Galstyan Jordi Bou-Torrent Irma Roig-Villanova Jaime E Martinez-Garcia Centre for Research in Agricultural Genomics （CRAG） Consortium CSIC-IRTA-UAB-UB Campus UAB Bellaterra 08193-Barcelona Spain present address： Department of Biology University of Washington Seattle WA 98195 USA present address： Universita＇ degli Studi di Milano Milano Italy Institucio Catalana de Recerca i Estudis Avan ats （ICREA） Pg. Llufs Companys 23 08010-Barcelona Spain

PAR1 is an atypical basic-helix-loop-helix （bHLH） protein that negatively regulates the shade avoidance syndrome in Arabidopsis thaliana acting as a transcriptional cofactor. Consistently with this function, PAR1 has to be in the nucleus to display biological activity. Previous structure-function analyses revealed that the N-terminal region of PAR1 drives the protein to the nucleus. However, truncated forms of PAR1 lacking this region still display biological activity, implying that PAR1 has additional mechanisms to localize into the nucleus. In this work, we compared the primary structure of PAR1 and various related and unrelated plant bHLH proteins, which led us to suggest that PAR1 contains a non-canonical nuclear localization signal （NLS） in the N-terminal region. By overexpressing truncated and mutated derivatives of PAR1, we have also investigated the importance of other regions of PAR1, such as the acidic and the extended HLH dimerization domains, for its nuclear localization. We found that, in the absence of the N-terminal region, a functional HLH domain is required for nuclear localization. Our results suggest the existence of a dual mechanism for PAR1 nuclear localization：（1） one mediated by the N-terminal non-consensus NLS and （2） a second one that involves interaction with other proteins via the dimerization domain,

关键词： nuclear localization signal basic-helix-loop-helix dimerization ability transcriptional cofactors Arabidopsis shade avoidance syndrome

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Ecological succession following forest decline in a xeric oak forest of south-central United States

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Journal of Plant Ecology 2016年第4期9卷 402-409页

作者： Devin P.Bendixsen Stephen W.Hallgren Jesse A.Burton Department of Natural Resource Ecology and Management Oklahoma State University008 Ag HallStillwaterOK 74074USA present address:Department of Biomolecular Sciences Boise State University1357 S.Brady St.BoiseID 83725USA present address:National Park Service 2680 Natchez Trace PkwyTupeloMS 38804USA

Aims The loss of canopy trees associated with forest decline can greatly influence the species composition and structure of a forest and have major impacts on the *** studied the changes in forest composition and structure 1 and 5 years following nearly total canopy mortality on several hundreds of hectares of xeric oak forests in south-central United *** the forests were within an ecotonal vegetation type composed of a mosaic of forest,savanna and grassland,we sought to learn whether forest decline areas would recover to forest or change to more open savanna and grassland conditions in the landscape pattern of *** low intensity fire shaped the vegetation type,we sought to learn whether fire would keep the decline areas *** The study was conducted in a xeric oak forest in east-central Oklahoma,*** located vegetation and regeneration surveys were conducted in decline and non-decline stands 1 and 5 years following nearly total canopy *** at breast height(DBH),regeneration and sprout origin were recorded for all woody *** FindingsThe major canopy species post oak(Quercus stellata Wangenh.),blackjack oak(Quercus marilandica Muenchh.)and black hickory(Carya texana Buckl.)suffered 85-92%mortality;however,minor canopy components experienced limited *** affected all size classes of canopy trees except those below 5 cm breast height *** was abundant regeneration of all species and fire seemed to maintain a high level of *** appeared to decrease the relative importance of stump sprouting and increase other types including root *** areas had abundant true seedlings,with stem origin from a root with the same diameter as the stem,which is very unusual for xeric oak *** height in decline areas was twice that of non-decline *** findings suggest that forest decline may lead to:(i)reduced oak dominance and species change in t

关键词： oak decline prescribed fire seedling stump sprouts drought

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SYNTHESIS OF 11 α-HYDROXY-AND 11β-CHLORO-ARTEMISININ

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Chinese Chemical Letters 1993年第2期4卷 99-100页

作者： Ying Li De Wen LU Hui Bin ZHANG Shanghai Institute of Materia Medica Academia SinicaShanghai 200031 present address:Department of Chemistry Changshu College.Changshu 215500 present address:Division of Medicinal Chemistry Chinese Pharmaceutical UniversityNanjing 210009

11α-Hydroxy-and 11β-chloro-artemisinin have been prepared from artemisinin

关键词： CHLORO-ARTEMISININ HYDROXY-AND 11 SYNTHESIS OF 11 OH 429

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A seismic gap on the Anninghe fault in western Sichuan, China

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Science China Earth Sciences 2008年第10期51卷 1375-1387页

作者： XueZe Wen Jun Fan GuiXi Yi YiWei Deng Feng Long Institute of Earthquake Science China Earthquake Administration Beijing 100036 China College of Information Engineering Chengdu University of Technology Chengdu 610059 China present address: Earthquake Administration of Sichuan Provinc

Through integrated analyses of time-varying patterns of regional seismicity, occurrence background of strong and large historical earthquakes along active faults, and temporal-spatial distribution of accurately reloca... 详细信息

关键词： Anninghe fault seismic quiescence seismic gap fault locking seismic potential

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Hosting certain facultative symbionts modulates the phenoloxidase activity and immune response of the pea aphid Acyrthosiphon pisum

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Insect Science 2021年第6期28卷 1780-1799页

作者： Chen Luo Maya Belghazi Antonin Schmitz Severine Lemauf Nicolas Desneux Jean-Christophe Simon Marylene Poirie Jean-Luc Gatti universite Cote d'Azur INRAECNRSUMR Institut Sophia Agrobiotech(ISA)Sophia AntipolisFrance INP UMR7051CNRSAix Marseille UniversiteMarseille13015France universite Cote d'Azur INRAECNRSUMR Institut Sophia Agrobiotech(ISA)06000 NiceFrance INRAE UMR IGEPP Domaine de la MotteLe RheuFrance present address:State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F UniversityYanglingShaanxi Province712100China

The pea aphid Acyrthosiphon pisum hosts different facultative symbionts(FS)which provide it with various benefits,such as tolerance to heat or protection against natural enemies(e.g.,fungi,parasitoid wasps).Here,we investigated whether and how the presence of certain FS could affect phenoloxidase(PO)activity,a key component of insect innate immunity,under normal and stressed *** this,we used clones of *** of difTerent genetic backgrounds(LLOl,YR2 and T3-8V1)lacking FS or harboring one or two(Regiella insecticola,Hamiltonella defensa,Serratia symbiotica Rickettsiella viridis).Gene expression and proteomics analyses of the aphid hemolymph indicated that the two *** POs,PPOl and PP02,are expressed and translated into *** level of PPO genes expression as well as the amount of PPO proteins and phenoloxidase activity in the hemolymph depended on both the aphid genotype and FS *** particular,*** and ***,but not ***-h ***,caused a sharp decrease in PO activity by interfering with both transcription and *** microinjection of different types of stressors(yeast,Escherichia coli,latex beads)in the YR2 lines hosting different symbionts affected the survival rate of aphids and,in most cases,also decreased the expression of PPO genes after 24 *** amount and activity of PPO proteins varied according to the type of FS and stressor,without clear corresponding changes in gene *** data demonstrate that the presence of certain FS influences an important component of pea aphid immunity.

关键词： Acyrthosiphon pisum facultative symbionts hemolymph immune response pea aphid phenoloxidases

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Arabidopsis thaliana AtUTr7 Encodes a Golgi-Localized UDP-Glucose/UDP-Galactose Transporter that Affects Lateral Root Emergence

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Molecular Plant 2012年第6期5卷 1263-1280页

作者： Michael Handford Cecilia Rodriguez-Furlan Lorena Marchan Marcelo Segura Daniela Gomez Elena Alvarez-Buyllal Guang-Yan Xiong Markus Pauly Ariel Orellana Department of Biology Faculty of Sciences University of Chile Santiago Chile present address: Department of Biochemistry University of Cambridge Cambridge CB2 1QW UK Centro de Biotecnologia Vegetal Facultad de Ciencias Biologicas Universidad Andres Bello Republica 217 Santiago Chile present address: Facultad de Ciencias Biologicas Universidad AndrOs Bello Vina del Mar Chile present address: Department of Pathobiology School of Veterinary Medicine University of Pennsylvania Philadelphia PA USA Instituto de Ecologia UNAM Mexico City Mexico Department of Plant and Microbial Biology University of California Berkeley CA 94720 USA FONDAP Center for Genome Regulation

Nucleotide sugar transporters （NSTs） are antiporters comprising a gene family that plays a fundamental role in the biosynthesis of complex cell wall polysaccharides and glycoproteins in plants. However, due to the limited number of related mutants that have observable phenotypes, the biological function（s） of most NSTs in cell wall biosynthesis and assembly have remained elusive. Here, we report the characterization of AtUTr7 from Arabidopsis （Arabidopsis thaliana （L.） Heynh.）, which is homologous to multi-specific UDP-sugar transporters from Drosophila melanogaster, humans, and Caenorhabditis elegans. We show that AtUTr7 possesses the common structural characteristics conserved among NSTs. Using a green fluorescent protein （GFP） tagged version, we demonstrate that AtUTr7 is localized in the Golgi apparatus. We also show that AtUTr7 is widely expressed, especially in the roots and in specific floral organs. Additionally, the results of an in vitro nucleotide sugar transport assay carried out with a tobacco and a yeast expression system suggest that AtUTr7 is capable of transferring UDP-Gal and UDP-GIc, but not a range of other UDP- and GDP-sugars, into the Golgi lumen. Mutants lacking expression of AtUTr7 exhibited an early proliferation of lateral roots as well as distorted root hairs when cultivated at high sucrose concentrations. Furthermore, the distribution of homogalacturonan with a low degree of methyl esterification differed in lateral root tips of the mutant compared to wild-type plants, although additional analytical procedures revealed no further differences in the composition of the root cell walls. This evidence suggests that the transport of UDP-Gal and UDP-GIc into the Golgi under conditions of high root biomass production plays a role in lateral root and root hair development.

关键词： nucleotide sugars nucleotide sugar transporters Golgi apparatus cell wall.

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Multicolor Photochromism of Polymolybdate-Citric Acid Composite Films

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Chinese Journal of Chemistry 2005年第8期23卷 1037-1041页

作者：王静张光晋杨文胜姚建年 Key Laboratory of Photochemistry Center for Molecular Science Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China present address: College of Chemistry Jilin University Changchun 130023 China

Photochromic polymolybdate-citric acid composite films were fabricated. It was found that after UV irradiation the composite films with different molar ratios of organic/inorganic components exhibited different colors. The UV-irradiated films showed dark blue, dark khaki and light sea green colors when the ratios were 1.0, 0.3 and 0.2, respectively. It was identified by Raman spectra that the polymolybdate species formed in the composite films after UV irradiation were sensitive to the ratios of the organic/inorganic components, thus resulting in the different colors of irradiated films. Citric acid played an important role during the photochromic process. Under UV light irradiation, it served as hole scavenger that suppresses the recombination of photogenerated electrons and holes to make the polymolybdates show UV light photochromism.

关键词： polymolybdate composite photochromism

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Spontaneous emission in non-local materials

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Light(Science & Applications) 2016年第1期5卷 1-10页

作者： Pavel Ginzburg Diane J Roth Mazhar E Nasir Paulina Segovia Alexey V Krasavin James Levitt Liisa M Hirvonen Brian Wells Klaus Suhling David Richards Viktor A Podolskiy Anatoly V Zayats Department of Physics King’s College LondonStrandLondon WC2R 2LSUK present address:School of Electrical Engineering Tel Aviv UniversityTel Aviv 69978Israel Department of Physics and Applied Physics University of Massachusetts LowellOne University AveLowellMA 01854USA present address:Department of Physics University of HartfordWest HartfordCT 06117USA

Light–matter interactions can be strongly modified by the surrounding ***,we report on the first experimental observation of molecular spontaneous emission inside a highly non-local metamaterial based on a plasmonic nanorod *** show that the emission process is dominated not only by the topology of its local effective medium dispersion,but also by the non-local response of the composite,so that metamaterials with different geometric parameters but the same local effective medium properties exhibit different Purcell factors.A record-high enhancement of a decay rate is observed,in agreement with the developed quantitative description of the Purcell effect in a non-local *** engineered material non-locality introduces an additional degree of freedom into quantum electrodynamics,enabling new applications in quantum information processing,photochemistry,imaging and sensing with macroscopic composites.

关键词： composite electromagnetic materials non-local optical properties plasmonic metamaterials quantum electrodynamics spontaneous emission

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Interactions of complement proteins C1q and factor H with lipid A and Escherichia coli:further evidence that factor H regulates the classical complement pathway

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Protein & Cell 2011年第4期2卷 320-332页

作者： Lee Aun Tan Andrew C.Yang Uday Kishore Robert B.Sim Medical Research Council Immunochemistry Unit Department of BiochemistryUniversity of OxfordSouth Parks RoadOxford OX13QUUK present address:Department of Immunology Faculty of MedicineImperial College LondonHammersmith HospitalDu Cane RoadLondon W120NNUK St Peter’s College New Inn Hall St.Oxford OX12DLUK present address:SUNY Downstate College of Medicine 450 Clarkson Avenue BrooklynNY 11203USA Weatherall Institute of Molecular Medicine John Radcliffe HospitalUniversity of OxfordHeadingtonOxford OX39DSUK present address:Center for Infection Immunity and Disease MechanismsBiosciencesSchool of Health Sciences and Social CareBrunel UniversityUxbridge UB83PH West LondonUK present address:Department of Pharmacology University of OxfordMansfield RoadOxford OX13QTUK

Proteins of the complement system are known to interact with many charged *** recently characterized binding of C1q and factor H to immobilized and liposomal anionic *** H inhibited C1q binding to anionic phospholipids,suggesting a role for factor H in regulating activation of the complement classical pathway by anionic *** extend this finding,we examined interactions of C1q and factor H with lipid A,a well-characterized activator of the classical *** report that C1q and factor H both bind to immobilized lipid A,lipid A liposomes and intact Escherichia coli *** H competes with C1q for binding to these ***,increasing the factor H:C1q molar ratio in serum diminished C4b fixation,indicating that factor H diminishes classical pathway *** recombinant forms of the Cterminal,globular heads of C1q A,B and C chains bound to lipid A and *** in a manner qualitatively similar to native C1q,confirming that C1q interacts with these targets via its globular head *** observations reinforce our proposal that factor H has an additional complement regulatory role of down-regulating classical pathway activation in response to certain *** is distinct from its role as an alternative pathway *** suggest that under physiological conditions,factor H may serve as a downregulator of bacterially-driven inflammatory responses,thereby finetuning and balancing the inflammatory response in infections with Gram-negative bacteria.

关键词： complement lipid A bacteria factor H C1q

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Rapid micro-immunohistochemistry

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Microsystems & Nanoengineering 2020年第1期6卷 285-294页

作者： Robert D.Lovchik David Taylor Govind Kaigala IBM Research Europe Saeumerstrasse 48803 RueschlikonSwitzerland present address:Eidgenossische Technische Hochschule Zurich Department of Mechanical and Process EngineeringSonneggstrasse 38092 ZurichSwitzerland

We present a new and versatile implementation of rapid and localized immunohistochemical staining of tissue ***(IHC)comprises a sequence of specific biochemical reactions and allows the detection of specific proteins in tissue *** the rapid implementation of IHC,we fabricated horizontally oriented microfluidic probes(MFPs)with functionally designed apertures to enable square and circular footprints,which we employ to locally expose a tissue to time-optimized sequences of different *** show that the two main incubation steps of IHC protocols can be performed on MDAMB468-1510A cell block sections in less than 30 min,compared to incubation times of an hour or more in standard *** analysis on the timescale of tens of minutes could potentially be applied during surgery,enabling clinicians to react in more dynamically and ***,this rapid IHC implementation along with conservative tissue usage has strong potential for the implementation of multiplexed assays,allowing the exploration of optimal assay conditions with a small amount of tissue to ensure high-quality staining results for the remainder of the sample.

关键词： versatile steps sections

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