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COPPER AMINE OXIDASE1 （CuAO1） of Arabidopsis thaliana Contributes to Abscisic Acid- and Polyamine-lnduced Nitric Oxide Biosynthesis and Abscisic Acid Signal Transduction

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Molecular Plant 2011年第4期4卷 663-678页

作者： Rinukshi Wimalasekera Corina Villar Tahmina Begum Giinther F. E. Scherer Leibniz Universitat Hannover Institute of Floriculture and Wood Science Section of Applied Molecular Physiology Herrenhauser Strasse 2 D-30419Hannover Germany present address: Institute of Plant Research ETH Universitatstr. 2 8092 Zurich Switzerland present address: ZMBP University of T~bingen Auf der Morgenstell 28 D-72076 Tubingen Germany

Polyamines （PA）, polyamine oxidases, copper amine oxidases, and nitric oxide （NO） play important roles in physiology and stress responses in plants. NO biosynthesis as a result of catabolism of PA by polyamine oxidases and copper amine oxidases may explain in part PA-mediated responses. Involvement of a copper amine oxidase gene, COPPER AMINE OXIDASEI （CuAO1）, of Arabidopsis was tested for its role in stress responses using the knockouts cuao1-1 and cuaol-2. PA-induced and ABA-induced NO production investigated bY fluorometry and fluorescence microscopy showed that the cuaol-1 and cuaol-2 are impaired in NO production, suggesting a function of CuAO1 in PA and ABA-mediated NO production. Furthermore, we found a PA-dependent increase in protein S-nitrosylation. The addition of PA and ABA also resulted in H2O2 increases, cuao1-1 and cuao1-2 showed less sensitivity to exogenous ABA supplementation during germination, seedling establishment, and root growth inhibition as compared to wild-type. In response to ABA treatment, expression levels of the stress-responsive genes RD29A and ADH1 were significantly lower in the knockouts. These observations characterize cuao1-1 and cuao1-2 as ABA-insensitive mutants. Taken together, our findings extend the ABA signal transduction network to include CuAO1 as one potential contributor to enhanced NO production by ABA.

关键词： COPPER AMINE OXIDASE1 polyamines nitric oxide abscisic acid signal transduction germination.

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Lensless diffractive imaging with ultra-broadbandtable-top sources: from infrared to extreme-ultravioletwavelengths

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Light(Science & Applications) 2014年第1期3卷 274-281页

作者： Stefan Witte Vasco T Tenner Daniel WE Noom Kjeld SE Eikema LaserLaB Amsterdam VU University Amsterdam1081 HV AmsterdamThe Netherlands present address:Huygens-Kamerlingh Onnes Laboratorium Universiteit Leiden2333 CA LeidenThe Netherlands

Lensless imaging is an approach to microscopy in which a high-resolution image of an object is reconstructed from one or more measured diffraction patterns,providing a solution in situations where the use of imaging optics is not ***,current lensless imaging methods are typically limited by the need for a light source with a narrow,stable and accurately known *** have developed a general approach to lensless imaging without spectral bandwidth limitations or sample *** use two time-delayed coherent light pulses and show that scanning the pulse-to-pulse time delay allows the reconstruction of diffraction-limited images for all the spectral components in the *** addition,we introduce an iterative phase retrieval algorithm that uses these spectrally resolved Fresnel diffraction patterns to obtain high-resolution images of complex extended *** demonstrate this two-pulse imaging method with octave-spanning visible light sources,in both transmission and reflection geometries,and with broadband extreme-ultraviolet radiation from a high-harmonic generation *** approach enables effective use of low-flux ultra-broadband sources,such as table-top high-harmonic generation systems,for high-resolution imaging.

关键词： coherent diffractive imaging extreme ultraviolet Fourier transform spectroscopy high-harmonic generation lensless imaging

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INFLUENCE OF SOIL MOISTURE AND VEGETATION ON CLIMATE CHANGES INDUCED BY THERMAL FORCING

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Acta meteorologica Sinica 1992年第1期6卷 58-69页

作者：刘永强叶笃正季劲钧 present address:Chinese Academy of Meteorological Sciences Beijing Institute of Atmospheric Physics Academia SinicaBeijing

A land-process scheme has been incorporated in a vertical one-dimensional time-dependent atmospheric model and numerical experiments have been performed with the coupled model to examine influences of soil wetness and vege- tation on climate changes associated to thermal *** is showed that response of land-surface temperature to the thermal forcing becomes small with increase of soil water content and vegetation ***,the response is more obvious in arid climate region than in humid *** result also shows that there exist two patterns of corre- sponding relation between variations in air temperature and humidity on the land surface in response to hydrologic and thermal focing.

关键词： soil moisture vegetation greenhouse effect climate change numerical experiment

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Experimental Modal Damping Identification of a Mechanical Structure Using Video Magnification Technique

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Sound & Vibration 2021年第2期55卷 131-140页

作者： Jaafar Hallal Mahmoud Fakih Hala Damerji Mohammad Hammoud Mehdi Chouman Mechanical Engineering Department Faculty of EngineeringLebanese UniversityBeirutLebanon Civil Engineering Department Faculty of EngineeringLebanese UniversityBeirutLebanon universite grenoble alpes CNRSGrenoble INP3SRGrenobleFrance SDM Research Group Mechanical Engineering DepartmentInternational University of BeirutBeirutLebanon Mechanical Engineering Department Lebanese International UniversityBekaaLebanon

Vibration can be introduced in all mechanical fields in our *** try to avoid its negative effect leading in some cases to deformation in the *** researches are dedicated to study the identification of damping especially in multi degree of freedom systems with particular attention to the source of energy *** focus on developing new tools or methods which may be used in real problems to obtain accurate results about the amount(or value)and the location of energy dissipation in the *** aim of this paper is to present an original procedure aims to experimentally determine the modal damping ratio of a mechanical *** proposed procedure consists of extracting the Frequency Response Function of the vibrating system using the video magnification method and then calculate the modal damping ratio using the 3-dB *** experimental measurements are carried out by giving an external force on a cantilever beam,then the modal damping ratios are extracted using motion *** obtained results show a relative error less than 4.2% between the experimental measurements and the analytical calculation for the Frequency Response Function(FRF)*** novelty of the paper is to combine the video magnification technique and the 3dB method in a procedure that aims to experimentally measure the modal damping of a mechanical *** proposed procedure in this paper represents the damping identification as a simple and easy engineering application.

关键词： Vibrations modal damping experimental measurement video magnification

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Timing Is Everything： Highly Specific and Transient Expression of a MAP Kinase Determines Auxin-Induced Leaf Venation Patterns in Arabidopsis

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Molecular Plant 2014年第11期7卷 1637-1652页

作者： Vera Stanko Concetta Giuliani Katarzyna Retzer Armin Djamei Vanessa Wahl Bernhard Wurzinger Cathal Wilson Erwin Heberle-Bors Markus Teige Friedrich Kragler Department of Plant Molecular Biology Max. F. Perutz Laboratories University of Vienna Dr. Bohrgasse 9/4 Vienna A-1030 Austria present address: Felix-Klein-Gymnasium Bottingerstraβe 17 D-37073 Gottingen Germany present address: Austrian Centre of Industrial Biotechnology Muthgasse 11 A-1190 Vienna Austria present address: Department of Applied Genetics and Cell Biology University of Natural Resources and Life Sciences Muthgasse 18 A-1190 Vienna Austria present address: Gregor Mendel Institute of Molecular Plant Biology Dr. Bohr-Gasse 3 1030 Vienna Austria Max Planck Institute of Molecular Plant Physiology Wissenschaftspark Golm Am Muhlenberg 1 D-14476 Potsdam-Golm Germany Department of Biochemistry Max. F. Perutz Laboratories University of Vienna Dr. Bohrgasse 9/5 Vienna A-1030 Austria present address: Department of Ecogenomics and Systems Biology University of Vienna Althanstr. 14 A-1090 Vienna Austria present address: Telethon Institute of Genetics and Medicine Via Pietro Castellino 11180131-Naples Italy Department of Ecogenomics and Systems Biology University of Vienna AIthanstr. 14 A-1090 Vienna Austria

Mitogen-activated protein kinase （MAPK） cascades are universal signal transduction modules present in all eukaryotes. In plants, MAPK cascades were shown to regulate cell division, developmental processes, stress responses, and hormone pathways. The subgroup A of Arabidopsis MAPKs consists of AtMPK3, AtMPK6, and AtMPK10. AtMPK3 and AtMPK6 are activated by their upstream MAP kinase kinases （MKKs） AtMKK4 and AtMKK5 in response to biotic and abiotic stress. In addition, they were identified as key regulators of stomatal development and patterning. AtMPKIO has long been considered as a pseudo-gene, derived from a gene duplication of AtMPK6. Here we show that AtMPKIO is expressed highly but very transiently in seedlings and at sites of local auxin maxima leaves. MPK10 encodes a functional kinase and interacts with the upstream MAP kinase kinase （MAPKK） AtMKK2. mpklO mutants are delayed in flowering in long-day conditions and in continuous light. Moreover, cotyledons of mpk10 and mkk2 mutants have reduced vein complexity, which can be reversed by inhibiting polar auxin transport （PAT）. Auxin does not affect AtMPKIO expression while treatment with the PAT inhibitor HFCA extends the expression in leaves and reverses the mpklO mutant phenotype. These results suggest that the AtMKK2-AtMPK10 MAPK module regulates venation complexity by altering PAT efficiency.

关键词： Arabidopsis MAP kinase leaf development polar auxin transport leaf venation pattern.

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Demonstration of tantalum as a structural material for MEMS thermal actuators

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Microsystems & Nanoengineering 2021年第1期7卷 85-97页

作者： Longchang Ni Ryan M.Pocratsky Maarten P.de Boer CMU Mechanical Engineering Dept. 5000 Forbes Ave.PittsburghPA 15213USA present address:Fischione Instruments 9003 Corporate CirExportPA 15632USA

This work demonstrates the processing,modeling,and characterization of nanocrystalline refractory metal tantalum(Ta)as a new structural material for microelectromechanical system(MEMS)thermal actuators(TAs).Nanocrystalline Ta films have a coefficient of thermal expansion(CTE)and Young’s modulus comparable to bulk Ta but an approximately ten times greater yield *** mechanical properties and grain size remain stable after annealing at temperatures as high as 1000°*** has a high melting temperature(Tm=3017℃)and a low resistivity(ρ=20µΩcm).Compared to TAs made from the dominant MEMS material,polycrystalline silicon(polysilicon,Tm=1414℃,ρ=2000µΩcm),Ta TAs theoretically require less than half the power input for the same force and displacement,and their temperature change is half that of *** TAs operate at a voltage 16 times lower than that of other TAs,making them compatible with complementary metal oxide semiconductors(CMOS).We selectα-phase Ta and etch 2.5-μm-thick sputterdeposited films with a 1μm width while maintaining a vertical sidewall profile to ensure in-plane movement of TA *** is 25 times thicker than the thickest reactive-ion-etchedα-Ta reported in the technical *** stress sensitivities to sputter parameters and to hydrogen incorporation are investigated and ***,a V-shaped TA is fabricated and tested in *** conventional actuation by Joule heating and passive self-actuation are as predicted by models.

关键词： thermal structural thick

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Fracture gradient prediction:an overview and an improved method

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Petroleum Science 2017年第4期14卷 720-730页

作者： Jincai Zhang Shang-Xian Yin Geomech Energy HoustonTXUSA North China Institute of Science and Technology YanjiaoBeijing 101601China present address:Sinopec Tech HoustonTXUSA

The fracture gradient is a critical parameter for drilling mud weight design in the energy industry. A new method in fracture gradient prediction is proposed based on analyzing worldwide leak-off test(LOT) data in offshore drilling. Current fracture gradient prediction methods are also reviewed and compared to the proposed method. We analyze more than 200 LOT data in several offshore petroleum basins and find that the fracture gradient depends not only on the overburden stress and pore pressure, but also on the depth. The data indicate that the effective stress coefficient is higher at a shallower depth than that at a deeper depth in the shale formations. Based on this finding,a depth-dependent effective stress coefficient is proposed and applied for fracture gradient prediction. In some petroleum basins, many wells need to be drilled through long sections of salt formations to reach hydrocarbon *** fracture gradient in salt formations is very different from that in other sedimentary rocks. Leak-off test data in the salt formations are investigated, and a fracture gradient prediction method is proposed. Case applications are examined to compare different fracture gradient methods and validate the proposed methods. The reasons why the LOT value is higher than its overburden gradient are also explained.

关键词： Fracture gradient prediction Leak-off test Breakdown pressure Mud loss Fracture gradient in salt

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The transcription factor MYB40 is a central regulator in arsenic resistance in Arabidopsis

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Plant Communications 2021年第6期2卷 44-55页

作者： Yun Chen Hong-Yang Wang Yi-Fang Chen College of Biological Sciences China Agricultural UniversityBeijingChina present address:Institute of Metabolism and Integrative Biology Fudan UniversityShanghaiChina

Arsenic is a metalloid that is toxic to ***(As(V)),the prevalent chemical form of arsenic,is a phosphate(Pi)analog and is incorporated into plant cells via Pi ***,we found that the MYB40 transcription factor played important roles in the control of Arabidopsis As(V)*** expression of MYB40 was induced by As(V)***40-overexpressing lines had an obvious As(V)-resistant phenotype and a reduced As(V)/Pi uptake rate,whereas myb40 mutants were sensitive to As(V)*** exposure to As(V),MYB40 directly repressed the expression of PHT1;1,which encodes a main Pi *** As(V)-resistant phenotypes of MYB40-overexpressing lines were impaired by overexpression of PHT1;1,demonstrating an epistatic genetic relationship between MYB40 and PHT1;***,overexpression of MYB40 enhanced,and disruption of MYB40 reduced,thiol-peptide *** exposure to As(V),MYB40 positively regulated the expression of PCS1,which encodes a phytochelatin synthase,and ABCC1 and ABCC2,which encode the major vacuolar phytochelatin ***,our data demonstrate that AtMYB40 acts as a central regulator of As(V)responses,providing a genetic strategy for enhancing plant As(V)tolerance and reducing As(V)uptake to improve food safety.

关键词： AtMYB40 arsenate uptake detoxification transcription

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An INDEHISCENT-Controlled Auxin Response Specifies the Separation Layer in Early Arabidopsis Fruit

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Molecular Plant 2016年第6期9卷 857-869页

作者： Kasper van Gelderen Martin van Rongen An'an Liu Anne Otten Remko Offringa Molecular and DeveLopmental Genetics Institute of Biology Leiden Leiden University Sylviusweg 72 2333 BE Leiden the Netherlands These authors contributed equally to this article. present address: Plant EcophysJology Institute of Environmental Biology Utrecht University 3584 CH Utrecht the Netherlands present address: Sainsbury Laboratory 47 Sateman Street Cambridge CB2 1LR UK

Seed dispersal is an important moment in the life cycle of a plant species. In Arabidopsis thaliana, it is dependent on transcription factor INDEHISCENT （IND）-mediated specification of a separation layer in the dehiscence zone found in the margin between the valves （carpel walls） and the central replum of the developing fruit. It was proposed that IND specifies the separation layer by inducing a local auxin minimum at late stages of fruit development. Here we show that morphological differences between the ind mutant and wild-type fruit already arise at early stages of fruit development, coinciding with strong IND expression in the valve margin. We show that IND-reduced PIN-FORMED3 （PIN3） auxin efflux carrier abundance leads to an increased auxin response in the valve margin during early fruit develop- ment, and that the concomitant cell divisions that form the dehiscence zone are lacking in ind mutant fruit. Moreover, IND promoter-driven ectopic expression of the AGC kinases PINOID （PID） and WAG2 induced indehiscence by expelling auxin from the valve margin at stages 14-16 of fruit development through increased PIN3 abundance. Our results show that IND, besides its role at late stages of Arabi- dopsis fruit development, functions at early stages to facilitate the auxin-triggered cell divisions that form the dehiscence zone.

关键词： seed dispersal polar auxin transport PINOID INDEHISCENT separation layer Arabidopsis

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Meromorphic Functions Sharing One Value with Their Derivatives Concerning the Difference Operator

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Communications in Mathematics and Statistics 2017年第4期5卷 407-427页

作者： Sujoy Majumder Department of Mathematics Katwa CollegeBurdwan 713130India present address:Department of Mathematics Raiganj UniversityRaiganjWest Bengal 733134India

Let f(z)beanon-constantmeromorphicfunctionoffiniteorder,c∈C\{0}andk∈*** f(z)and f(k)(z+c)share1CM(IM),f(z)and f(z+c)share∞*** N(r,0;f)=S(r,f)(N(r,0;f(z))+N(r,0;f(k)(z+c))=S(r,f)),then either f(z)≡f(k)(z+c)or f(z)is a solution of the following equation:f((z+c)−1=a(z)(f(z)−1))f(z)+1 a(z)),and N(r,0;f(z)+1 a(z))=S(r,f)(f′(z+c)−1=a(z)(f(z)−1)(f(z)+1 a(z)))where a(z)(≡−1,0,∞)(a(z)(≡0,∞))is a meromorphic function satisfying T(r,a)=S(r,f).Also we exhibit some examples to show that the conditions of our results are the best possible.

关键词： Uniqueness Meromorphic function·Shift operator Difference polynomial Sharing values

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