COPPER AMINE OXIDASE1 （CuAO1） of Arabidopsis thaliana Contributes to Abscisic Acid- and Polyamine-lnduced Nitric Oxide Biosynthesis and Abscisic Acid Signal Transduction

作     者：Rinukshi Wimalasekera Corina Villar Tahmina Begum Giinther F. E. Scherer 

作者机构：Leibniz Universitat Hannover Institute of Floriculture and Wood Science Section of Applied Molecular Physiology Herrenhauser Strasse 2 D-30419Hannover Germany Present address: Institute of Plant Research ETH Universitatstr. 2 8092 Zurich Switzerland Present address: ZMBP University of T~bingen Auf der Morgenstell 28 D-72076 Tubingen Germany 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2011年第4卷第4期

页      面：663-678页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：Deutsche Forschungsgemeinschaft grant 

主　　题：COPPER AMINE OXIDASE1 polyamines nitric oxide abscisic acid signal transduction germination. 

摘      要：Polyamines （PA）, polyamine oxidases, copper amine oxidases, and nitric oxide （NO） play important roles in physiology and stress responses in plants. NO biosynthesis as a result of catabolism of PA by polyamine oxidases and copper amine oxidases may explain in part PA-mediated responses. Involvement of a copper amine oxidase gene, COPPER AMINE OXIDASEI （CuAO1）, of Arabidopsis was tested for its role in stress responses using the knockouts cuao1-1 and cuaol-2. PA-induced and ABA-induced NO production investigated bY fluorometry and fluorescence microscopy showed that the cuaol-1 and cuaol-2 are impaired in NO production, suggesting a function of CuAO1 in PA and ABA-mediated NO production. Furthermore, we found a PA-dependent increase in protein S-nitrosylation. The addition of PA and ABA also resulted in H2O2 increases, cuao1-1 and cuao1-2 showed less sensitivity to exogenous ABA supplementation during germination, seedling establishment, and root growth inhibition as compared to wild-type. In response to ABA treatment, expression levels of the stress-responsive genes RD29A and ADH1 were significantly lower in the knockouts. These observations characterize cuao1-1 and cuao1-2 as ABA-insensitive mutants. Taken together, our findings extend the ABA signal transduction network to include CuAO1 as one potential contributor to enhanced NO production by ABA.