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Natural stilbenes effects in animal models of Alzheimer’s disease

Neural Regeneration Research 2020年第5期15卷 843-849页

作者： Aline Freyssin Guylène Page Bernard Fauconneau Agnès Rioux Bilan University of Poitiers EA3808 NEUVACOD (Neurovascular Unit and Cognitive Disorders)P?le Biologie Santé

Alzheimer’s disease is one of the most frequent neurodegenerative *** pathology is characterized by protein aggregates,mainly constituted by amyloid peptide and tau,leading to neuronal death and cognitive *** currently proposed to treat this pathology do not prevent neurodegenerative processes and are mainly symptomatic ***,stilbenes presenting multiple pharmacological effects could be good potential therapeutic *** aim of this review is to gather the more significant papers among the broad literature on this topic,concerning the beneficial effects of stilbenes (resveratrol derivatives) in animal models of Alzheimer’s ***,numerous studies focus on cellular models,but an in vivo approach remains of primary importance since in animals (mice or rats,generally),bioavailability and metabolism are taken into account,which is not the case in in vitro ***,examination of memory ability is feasible in animal models,which strengthens the relevance of a compound with a view to future therapy in *** paper is addressed to any researcher who needs to study untested natural stilbenes or who wants to experiment the most effective natural stilbenes in largest animals or in *** review shows that resveratrol,the reference polyphenol,is largely studied and seems to have interesting properties on amyloid plaques,and cognitive ***,some resveratrol derivatives such as gnetin C,trans-piceid,or astringin have never been tested on ***,pterostilbene is of particular interest,by its improvement of cognitive disorders and its neuroprotective *** could be relevant to evaluate this molecule in clinical trials.

关键词： Alzheimer's disease amyloid animal models cognitive impairment inflammation natural stilbenes neuroprotection resveratrol tau

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Robust and low-cost interrogation technique for integrated photonic biochemical sensors based on Mach–Zehnder interferometers

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Photonics Research 2016年第2期4卷 57-60页

作者： V.Toccafondo C.J.Oton Consorzio Nazionale Interuniversitario per le Telecomunicazioni Scuola Superiore sant'Anna

We describe and experimentally demonstrate a measuring technique for Mach–Zehnder interferometer(MZI)based integrated photonic biochemical sensors. Our technique is based on the direct measurement of phase changes between the arms of the MZI, achieved by signal modulation on one of the arms of the interferometer together with pseudoheterodyne detection, and it allows us to avoid the use of costly equipment such as tunable light sources or spectrum analyzers. The obtained output signal is intrinsically independent of wavelength, power variations, and global thermal variations, making it extremely robust and adequate for use in real conditions. Using a silicon-on-insulator MZI, we demonstrate the real-time monitoring of refractive index variations and achieve a detection limit of 4.1 × 10^(-6)refractive index units(RIU).

关键词： Robust and low-cost interrogation technique for integrated photonic biochemical sensors based on Mach Zehnder interferometers

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Minigene Analysis of the c.743 + 40A >G Intronic Variant in the <i>CFTR</i>Gene

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Health 2014年第12期6卷 1352-1360页

作者： Ayman El-Seedy Raed Farhat Marie-Claude Pasquet Alain Kitzis Véronique Ladeveze Génétique Moléculaire de Maladies Rares P&ocirc le biologie santé Université de Poitiers Poitiers France

Background: Since the identification of the cystic fibrosis transmembrane regulator (CFTR) gene in 1989, many polymorphisms have been identified in cystic fibrosis (CF) or CFTR-related disorders (CFTR-RDs) patients and still remain to be characterized at the molecular level. These polymorphisms are difficult to classify as pathogenic or non-disease causing because the polymorphisms are either located in the coding region, but are synonymous, or are found in the intronic regions. Here we investigated the potential impact of the c.743 + 40A > G polymorphism within CFTR intron 6 on the alternative splicing. Indeed, this variant has been observed frequently in our examined patients. Moreover, a family carrying this variant exhibited CFTR-RD phenotype. Methods: By denaturing high pressure liquid phase chromatography (DHPLC) and sequencing, thirty of 293 subjects French origin carried the c.743 + 40A > G variant. Of these, 16 patients were affected by CF or CFTR-RD. Wild-type sequences and mutant CFTR intron 6 and its boundaries were inserted into the pTBNdeI hybride minigene and expressed in three different cell lines. After RT-PCR analysis of mRNA using specific primers, sequences of the minigene transcripts were obtained. Results: No aberrant splicing was detected with minigene carrying c.743 + 40A > G variant in all transfected cell lines. However, an alternative splicing in the positive control was detected with a minigene carrying the c.1392G > T + 1G > T mutation: 5 nucleotides were deleted from mRNA sequences, indicating that used cell lines are appropriate for studying the splicing. Conclusion: Transient transfections of a minigene containing the c.743 + 40A > G polymorphism showed no splicing errors, and thus this intronic alteration was finally classified as non-pathogenic. As it is always associated with c.2562T > G and c.4389G > A, or TG12-7T poly-morphisms, further experiments are needed to determine the role of these complex alleles in disease pathogenesis.

关键词： Cystic Fibrosis Polymorphism Complex Allele Hybrid Minigene Splicing

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Hepatocyte nuclear factor 4-alpha involvement in liver and intestinal inflammatory networks

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World Journal of Gastroenterology 2014年第1期20卷 22-30页

作者： Jean-Philippe Babeu Franois Boudreau Département d’ Anatomie et de biologie Cellulaire Faculté de Médecine et des Sciences de la SantéUniversité de SherbrookeSherbrooke J1E 4K8QuébecCanada

Hepatocyte nuclear factor 4-alpha (HNF4-α) is a nuclear receptor regulating metabolism, cell junctions, differentiation and proliferation in liver and intestinal epithelial cells. Mutations within the HNF4A gene are associated with human diseases such as maturity-onset diabetes of the young. Recently, HNF4A has also been described as a susceptibility gene for ulcerative colitis in genome-wide association studies. In addition, specific HNF4A genetic variants have been identified in pediatric cohorts of Crohn’s disease. Results obtained from knockout mice supported that HNF4-α can protect the intestinal mucosae against inflammation. However, the exact molecular links behind HNF4-α and inflammatory bowel diseases remains elusive. In this review, we will summarize the current knowledge about the role of HNF4-α and its isoforms in inflammation. Specific nature of HNF4-α P1 and P2 classes of isoforms will be summarized. HNF4-α role as a hepatocyte mediator for cytokines relays during liver inflammation will be integrated based on documented examples of the literature. Conclusions that can be made from these earlier liver studies will serve as a basis to extrapolate correlations and divergences applicable to intestinal inflammation. Finally, potential functional roles for HNF4-α isoforms in protecting the intestinal mucosae from chronic and pathological inflammation will be presented.

关键词： Hepatocyte nuclear factor 4-alpha Inflammatory bowel diseases Colitis-associated cancer Gastrointestinal tract Intestinal epithelium barrier Inflammation

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Anthelmintic activity of Saba senegalensis(***.) Pichon (Apocynaceae) extract against adult worms and eggs of Haemonchus contortus

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Asian Pacific Journal of Tropical Biomedicine 2016年第11期6卷 945-949页

作者： Mohamed Bonewendé Belemlilga Aristide Traoré Sylvin Ouédraogo Adama Kaboré Hamidou Hamadou Tamboura Innocent Pierre Guissou Laboratoire de Développement du Médicament Centre de recherche sur le médicament Ecole Doctorale de la SantéUniversité de Ouagadougou Ecole Doctorale de Santé Département Médecine Pharmacopée Traditionnelles–Pharmacie (MEPHATRA-Ph) Institut de Recherche en Sciences de la Santé (IRSS/CNRST) Département Productions Animales Institut de l’Environnement et de Recherches Agricoles(INERA/CNRST)

Objective: To evaluate the anthelmintic property of Saba senegalensis(***) Pichon(Apocynaceae)(S. senegalensis) on Haemonchus contortus that is traditionally used in Burkina Faso for its gastrointestinal parasites ***: The lyophilized aqueous decoction of leaves of S. senegalensis at concentrations of 0.10, 1.00, 3.00, 10.00 and 15.00 mg/m L was used on eggs and adult worms of Haemonchus contortus collected from gastrointestinal tract of small ***: The LC_(50) on adult worms was 6.79 mg/m L and 3.25 mg/m L for the leaves of S. senegalensis and the levamisole(reference drug), respectively. Inhibition of hatching assay showed a concentration-dependent manner with an inhibition of 93.63% at the concentration of 15.00 mg/m L of S. ***: These results indicate that the aqueous extract of S. senegalensis possesses an anthelmintic property and may justify its use in traditional medicine for the treatment of gastrointestinal parasites.

关键词： Saba senegalensis Anthelmintic properties Haemonchus contortus In vitro Burkina Faso

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Charcot-Marie-Tooth-1A and sciatic nerve crush rat models:insights from proteomics

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Neural Regeneration Research 2023年第6期18卷 1354-1363页

作者： Zeina Msheik Stephanie Durand Emilie Pinault Martial Caillaud Laetitia Vignaud Fabrice Billet Mohamed El Massry Alexis Desmoulière UR20218 NeurIT(NEURopathies périphériques et Innovation Thérapeutique) University of LimogesLimogesFrance BISCEm(biologie Intégrative santéChimie Environnement)Platform US 42 Inserm/UAR 2015 CNRSUniversity of LimogesLimogesFrance UMR 1308 Inserm/CHU–CAPTuR(Contrôle de l’Activation cellulaire Progression Tumorale et Résistance thérapeutique)University of LimogesLimogesFrance Inserm UMR1235–TENS(The Enteric Nervous System in Gut and Brain Diseases) University of NantesNantesFrance

The sensorimotor and histological aspects of peripheral neuropathies were already studied by our team in two rat models:the sciatic nerve crush and the Charcot-Marie-Tooth-1A *** this study,we sought to highlight and compare the protein signature of these two pathological ***,the identification of protein profiles in diseases can play an important role in the development of pharmacological *** fact,Charcot-Marie-Tooth-1A rats develop motor impairments that are more severe in the hind ***,for the first time,protein expression in sciatic nerve of Charcot-Marie-Tooth-1A rats was ***,distal sciatic nerves were collected from Charcot-Marie-Tooth-1A and uninjured wild-type rats aged 3 *** protein extraction,sequential window acquisition of all theoretical fragment ion spectra liquid chromatography and mass spectrometry was employed.445 proteins mapped to Swiss-Prot or trEMBL Uniprot databases were identified and *** these,153 proteins showed statistically significant differences between Charcot-Marie-Tooth-1A and wild-type *** majority of these proteins were overexpressed in *** clustering and functional enrichment using Gene Ontology were used to group these proteins based on their biological effects concerning Charcot-Marie-Tooth-1A ***,proteomic characterization of wild-type rats subjected to sciatic nerve crush was performed sequential window acquisition of all theoretical fragment ion spectra liquid chromatography and mass *** month after injury,distal sciatic nerves were collected and analyzed as described *** of 459 identified proteins,92 showed significant differences between sciatic nerve crush and the uninjured wild-type rats used in the first *** results suggest that young adult Charcot-Marie-Tooth-1A rats(3 months old)develop compensatory mechanisms at the level of redox balance,protein folding,myelination,and

关键词： Charcot-Marie-Tooth-1A endoplasmic reticulum Gene Ontology neurogenesis oxidative stress proteomics rat repair sciatic nerve crush SWATH-MS

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Associations between physical activity levels and ATPase inhibitory factor 1 concentrations in older adults

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Journal of Sport and Health Science 2024年第3期13卷 409-418页

作者： Jérémy Raffin Yves Rolland Annelise Genoux Guillaume Combes Mikael Croyal Bertrand Perret Sophie Guyonnet Bruno Vellas Laurent O.Martinez Philipe de Souto Barreto For the MAPT/DSA Group Institut du Vieillissement Gérontopôle de ToulouseCentre Hospitalo-Universitaire de ToulouseToulouse 31000France Centre d'Epidémiologie et de Recherche en santédes Populations UnitéMixte de Recherche 1295Institut National de la Santéet de la Recherche MédicaleUniversitéToulouse-III-Paul-SabatierToulouse 31000France LimitAging Team Institut des Maladies Métaboliques et CardiovasculairesUnitéMixte de Recherche 1297Institut National de la Santéet de la Recherche MédicaleUniversitéToulouse III-Paul SabatierToulouse 31432France Service de Biochimie Pôle de biologieHôpital de PurpanCentre Hospitalo-Universitaire de ToulouseToulouse 31300France L'Institut du Thorax Institut National de la Santéet de la Recherche MédicaleCentre National de la Recherche ScientifiqueCentre Hospitalo-Universitaire de NantesNantes UniversitéNantes 44000France BioCore US16Structure Fédérative de Recherche BonamyInstitut National de la Santéet de la Recherche MédicaleCentre National de la Recherche ScientifiqueCentre Hospitalo-Universitaire de NantesNantes UniversitéNantes 44000France Plate-forme de spectrométrie de masse Centre de Recherche en Nutrition HumaineOuestNantes 44000France 不详

Background:Adenosine triphosphatase inhibitory factor 1(IF1)is a key protein involved in energy ***1 has been linked to various agerelated diseases,although its relationship with physical activity(PA)remains ***,the apolipoprotein A-I(apoA-I),a PA-modulated lipoprotein,could play a role in this relationship because it shares a binding site with IF1 on the cell-surface ATP *** examined here the associations between chronic PA and plasma IF1 concentrations among older adults,and we investigated whether apoA-I mediated these ***:In the present work,1096 healthy adults(63.8%females)aged 70 years and over who were involved in the Multidomain Alzheimer Prevention Trial study were ***1 plasma concentrations(square root of ng/mL)were measured at the 1-year visit of the Multidomain Alzheimer Prevention Trial,while PA levels(square root of metabolic equivalent task min/week)were assessed using questionnaires administered each year from baseline to the 3-year *** linear regressions were performed to investigate the associations between the first-year mean PA levels and IF1 *** analyses were conducted to examine whether apoA-I mediated these *** linear regressions were carried out to investigate whether the 1-year visit IF1 concentrations predicted subsequent changes in ***:Multiple linear regressions indicated that first-year mean PA levels were positively associated with IF1 concentrations(B=0.021;SE=0.010;p=0.043).Mediation analyses revealed that about 37.7%of this relationship was mediated by apoA-I(B_(ab)=0.008;SE=0.004;p=0.023).Longitudinal investigations demonstrated that higher concentrations of IF1 at the 1-year visit predicted a faster decline in PA levels over the subsequent 2 years(time×IF1:B=0.148;SE=0.066;p=0.025).Conclusion:This study demonstrates that regular PA is associated with plasma IF1 concentrations,and it suggests that apoA-I partly med

关键词： Aging Apolipoprotein Bioenergetics Exerkine Mitochondria

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Fast Fatty Acid Analysis by Core-Shell Reversed-Phase Liquid Chromatography Coupled to Evaporative Light-Scattering Detector

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Food and Nutrition Sciences 2017年第12期8卷 1051-1062页

作者： Florence Hubert Céline Loiseau Francoise Ergan Gaelle Pencréac’h Laurent Poisson Laboratoire Mer MoléculesSanté(EA 2160)Le Mans UniversitéIUT de LavalLavalFrance

A high-performance liquid chromatography analysis method with an evaporative light-scattering detector has been developed for the separation and quantitative analysis of free fatty acids in biological matrices. Core-shell reversed-phase high-performance liquid chromatography separation of 10 free fatty acids is achieved within 10.5 min using a methanol/water (0.05% trifluoroacetic acid) eluent gradient. After optimization, the drift tube and nebulization temperature of the evaporative light-scattering detector was set at 35°C, nitrogen flow-rate at 1.1 standard liter per minute and column temperature at 25°C. All calibration curves showed good regression (r2 > 0.9975). A validation procedure following the International Conference on Harmonisation guidelines was implemented to certify the method. Relative standard deviations did not exceed 1.5% and 4.25% for repeatability and reproducibility respectively.

关键词： Free Fatty Acids HPLC Evaporative Light-Scattering Detector Core-Shell Technology Method Validation

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Capacitive actuation and switching of add–drop graphene-silicon micro-ring filters

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Photonics Research 2017年第6期5卷 274-278页

作者： TOMMASO CASSESE MARCO ANGELO GIAMBRA VITO SORIANELLO GABRIEle DE ANGELIS MICHEle MIDRIO MARIANNA PANTOUVAKI JORIS VAN CAMPENHOUT INGE ASSELBERGHS CEDRIC HUYGHEBAERT ANTONIO D'ERRICO MARCO ROMAGNOLI Scuola Superiore sant'Anna-Te CIP Institute CNIT-Consorzio Nazionale Interuniversitario per le Telecomunicazioni CNIT-Consorzio Nazionale Interuniversitario per le Telecomunicazioni Università degli Studi di Udine Imec Ericsson Research

We propose and experimentally demonstrate capacitive actuation of a graphene–silicon micro-ring add/drop filter. The mechanism is based on a silicon–SiO_2–graphene capacitor on top of the ring waveguide. We show the capacitive actuation of the add/drop functionality by a voltage-driven change of the graphene optical absorption. The proposed capacitive solution overcomes the need for continuous heating to keep tuned the filter's in/out resonance and therefore eliminates "in operation" energy consumption.

关键词： MRR drop graphene-silicon micro-ring filters Capacitive actuation and switching of add

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Validity of the French version of Catquest-9SF and use of an electronic notepad for entering patient-reported outcome measures

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Eye and Vision 2021年第1期8卷 92-100页

作者： Gregory Katz Alexandra Rouquette François Lignereux Thierry Mourgues Michel Weber Mats Lundström Chair of Innovation&Value in Health University of Paris School of MedicineParisFrance Value-Based Health Care Consortium ParisFrance Prom-Time ParisFrance Service de santéPublique et d’Épidémiologie AP-HP Paris-SaclayLe Kremlin-BicêtreFrance Centre de recherche enÉpidémiologie et santédes Populations InsermUniversitéParis-SaclayVillejuifFrance Ophtalmologie Chénieux Polyclinique de LimogesLimogesFrance Institut Ophtalmologique Sourdille-Atlantique Elsan SantéAtlantiqueNantesFrance Ophthalmology Department Centre Hospitalier-Universitaire de NantesNantesFrance Department of Clinical Sciences OphthalmologyFaculty of MedicineLund UniversityLundSweden

Background:The Catquest-9SF questionnaire is a patient reported outcome measure that quantifies the visual benefits from cataract *** purpose of this study was to translate and adapt the Catquest-9SF questionnaire for France,to assess its psychometric properties via Rasch analysis,and to assess its validity when completed using an electronic ***:The Catquest-9SF questionnaire was translated following the guidelines of the International Society for Pharmacoeconomics and Outcomes ***-9SF and clinical data were collected from patients before and after routine cataract *** questionnaire data were collected via an electronic *** analysis was performed to assess psychometric properties,and sensitivity to change was analysed for patients with complete paired pre-and post-operative ***:A complete filled-in preoperative questionnaire was obtained for 848 *** analysis showed good precision(person separation:2.32,person reliability:0.84),ordered category probability curves,no item misfit,and *** respondents were slightly more able than the level of item difficulty(targeting:−1.12 logits).Sensitivity was analysed on 211 paired questionnaires,and the postoperative questionnaires showed a clear ceiling *** effect size was *** use of an electronic notepad for completing the questionnaire worked out very well after some ***:The French version of Catquest-9SF has good psychometric properties and is suitable for use in French-speaking *** use of the Catquest-9SF questionnaire in an electronic format showed good validity.

关键词： Cataracts Patient-related outcome measures Quality of life Questionnaire Value-based health care Catquest-9SF

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