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Ultrastructural analysis of neuronal synapses using state-of-the-art nano-imaging techniques

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Neuroscience Bulletin 2012年第4期28卷 321-332页

作者： Changlu Tao Chenglong Xia Xiaobing Chen Z. Hong Zhou Guoqiang Bi Center for integrative Imaging Hefei National Laboratory for Physical Sciences at the Microscale & School of LifeSciences University of Science and Technology of China Hefei 230027 China Laboratory of neurobiology National Institute of Neurological Disorders and Stroke National Institutes of HealthBethesda 20892 USA Department of Microbiology Immunology & Molecular Genetics University of California Los Angeles Los Angeles CA90095-7364 USA

Neuronal synapses are functional nodes in neural *** organization and activity define an individual＇s level of intelligence,emotional state and mental *** in the structure and efficacy of synapses are the biological basis of learning and ***,investigation of the molecular architecture of synapses has been impeded by the lack of efficient techniques with sufficient *** developments in state-of-the-art nano-imaging techniques have opened up a new window for dissecting the molecular organization of neuronal synapses with unprecedented ***,we review recent technological advances in nano-imaging techniques as well as their applications to the study of synapses,emphasizing super-resolution light microscopy and 3-dimensional electron tomography.

关键词： synaptic architecture nano-imaging super-resolution imaging STED microscopy STORM PALM cryoET

Effects of Nourishing “Yin”-Removing “Fire” Chinese Herb Mixture on the Expression of GABAB Receptors in Hypothalamus of Precocious Puberty Female Rats

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Chinese Medicine 2016年第2期7卷 55-66页

作者： Zhejun Chen Zehui Meng Shiran Wang Liting Zhu Zhanzhuang Tian Department of integrative Medicine and neurobiology Institute of Acupuncture Research Institutes of Brain Science Collaborative Innovation Center for Brain Science State Key Laboratory of Medical Neurobiology Shanghai Medical College of Fudan University Shanghai China

Objectives: To investigate the potential role of GABAB Receptors (GABABRs) involved in the effect of Nourishing “Yin”-Removing “Fire” Chinese herb mixture (TCM) treatment on precocious puberty. Methods: Female Sprague-Dawley rats were randomly divided into four groups: normal (N), central precocious puberty (CPP) model (M), CPP fed with normal saline (S) and CPP fed with Nourishing “Yin”-Removing “Fire” Chinese herb mixture (TCM). Rats of postnatal day 5 were given a single subcutaneous injection of 240 μg danazol to establish CPP model rats. Rats of S and TCM groups were continuously administered with saline or nourishing “Yin”-removing “Fire” Chinese herb mixture since postnatal day 15. The expression of GABABRs was detected by means of real-time PCR and immunohistochemistry. Results: The expression of hypothalamic GnRH mRNA in M was significantly increased on the day of pre-puberty when compared with that of N (P B1 receptor (GABABR1) immunoreactive cells in the arcuate nucleus (ARN) was decreased in M when compared with that of N (P B2 receptor (GABABR2) immunoreactive cells in the ARN, MS and diagnonal band of broca (DBB) were decreased in M when compared with those of N (P < 0.05) and increased in TCM compared

关键词： Precocious Puberty GABAB Receptors Chinese Herb Medicine Danazol Rats

Recent progresses in molecular postharvest biology

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Molecular Horticulture 2022年第1期2卷 263-265页

作者： Su‑Sheng Gan Plant Biology section School of Integrative Plant ScienceCornell UniversityIthacaNY 14853USA

Postharvest biology of horticultural crops is a scientific discipline of the biological processes that occur in horticultural crops after *** is the foundation for practical strategies and technology for prolonging the postharvest longevity of *** postharvest longevity includes storage,logistics and transportation,and shelf *** is estimated that up to 70%of produce are lost after *** of postharvest biological processes will allow us to devise ways to control these processes for improving the produce quality(and quantity)and reducing the economic loss.

关键词： shelf crops harvest

Split intein-mediated protein trans-splicing to express large dystrophins

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四川生理科学杂志 2024年第7期46卷 1627-1627页

作者： Hichem Tasfaout Department of Neurology University of Washington School of Medicine Seattle WA USA Senator Paul D. Wellstone Muscular Dystrophy Specialized Research Center University of Washington School of Medicine Seattle WA USA Department of Biochemistry University of Washington School of Medicine Seattle WA USA Center for Translational Muscle Research University of Washington Seattle WA USA Department of Bioengineering College of Engineering and School of Medicine University of Washington Seattle WA USA Department of Infectious Diseases/Virology Section Viral Vector Technologies Medical Faculty and Faculty of Engineering Sciences Center for Integrative Infectious Disease Research (CIID) University of Heidelberg Heidelberg Germany BioQuant University of Heidelberg Heidelberg Germany German Center for Infection Research (DZIF) and German Center for Cardiovascular Research (DZHK) Heidelberg Germany

Gene replacement using adeno-associated virus(AAV)vectors is a promising therapeutic approach for many diseases1,***,this therapeutic modality is challenged by the packaging capacity of AAVs(approximately 4.7 kilobases)3,limiting its application for disorders involving large coding sequences,such as Duchenne muscular dystrophy,with a 14 kilobase messenger RNA.

关键词： Split bases limiting

The analgesic effect of aspirin-triggered 15-epi-lipoxin A4 in cancer-induced bone pain rats associated with MAPK signals

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The analgesic effect of aspirin-triggered 15-epi-lipoxin A4 ...

Phylogeny of nucleus medianus of the posterior tubercle in ray-finned fishes

中国神经科学学会第十届全国学术会议

作者： Shan HU Qi-Liang MAO-YING Jun WANG Zhi-Fu WANG Wen-Li MI Jian-Wei JIANG Gen-Cheng WU Yan-Qing WANG Department of integrative Medicine and neurobiology Shanghai Medical College Institute of Acupuncture ResearchInstitutes of Brain Science State Key Laboratory of Medical Neurobiology Fudan University

Objective The neuroinflammation play an important role in cancer-induced bone pain(CIBP) ***-triggered 15-epi-lipoxin A4(ATL) is one of the analogues of lipoxins,which play an important role in anti-inflammatory and pro-resolution *** this report,we explore the mechanism of ATL in CIBP pain *** The paw withdrawal thresholds(PWT) were tested by von-Frey *** expression of proinflammatory cytokines were examined by Real-Time *** phosphorylated MAPK signals were tested by Western ***(1) Intrathecal with ATL could alleviate the mechanical allodynia in *** the day 11 after surgery,intrathecal with different dose of ATL showed increased *** with 10 μg/kg or 40 ug/kg ATL showed increased PWT and the dose of 40 μg/kg ATL showed a better effect than the vehicle(P<0.01).(2) Real-Time PCR analysis revealed that the expression of IL-1β and TNF-αmRNA were significant decreased(P<0.05) at the 2 hour after intrethecal with 0.1ug ATL.3) Western Blot probes the phosphorylated ERK,p38 and JNK showed that the increased phosphorylated ERK,p38 and JNK in CIBP were prevented by intrathecal with 0.1 μg ATL at the 2 *** The results suggested that ATL exert analgesic effect in CIBP and mainly associated with suppressing the phosporylated MAPK signals.

关键词： aspirin-triggered 15-epi-lipoxin A4 neuroinflammation cancer-induced bone pain MAPK signals

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integrative Zoology 2009年第1期4卷 134-152页

作者： R.Glenn NORTHCUTT Laboratory of Comparative neurobiology Scripps Institution of Oceanographyand Department of NeurosciencesUniversity of CaliforniaLa JollaUSA

The brains of ray-finned fishes form a morphocline of increasing complexity,from cladistians through *** is particularly apparent in the posterior tubercle of the *** cladistians,the posterior tubercle consists of a periventricular nucleus and a migrated nucleus medianus that is fused across the *** more advanced ray-finned fishes,such as gars and bowfins,the posterior tubercle comprises numerous additional mi-grated nuclei,termed the preglomerular complex,in addition to a more well developed nucleus *** teleosts,the most derived ray-finned fishes,there is an even more elaborate preglomerular complex,but there is no recognizable nucleus *** an attempt to explain the variation in the posterior tubercle of the diencephalon in ray-finned fishes,the immunohistochemistry and connections of nucleus medianus were examined in cladistians,gars and *** each of these taxa,nucleus medianus exhibits large numbers of calretinin-positive neurons and has ascending projections that terminate in several divisions of the *** teleosts,such as goldfish,also exhibit numerous cell groups in the posterior tubercle that are rich in calretinin,none of these cell groups has connections that are comparable to those of nucleus medianus in non-teleost ray-finned *** is possible,therefore,that nucleus medianus was lost with the origin of teleosts.

关键词： bichirs evolution gars nucleus medianus posterior tubercle sturgeons.

Selective ablation of type 3 adenylyl cyclase in somatostatin-positive interneurons produces anxiety-and depression-like behaviors in mice

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World Journal of Psychiatry 2021年第2期11卷 35-49页

作者： Xiao-Yu Yang Zhao-Liang Ma Daniel R Storm Hong Cao Yu-Qiu Zhang Department of Translational Neuroscience Jing’an District Centre Hospital of ShanghaiState Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain ScienceInstitutes of Brain ScienceInstitutes of Integrative MedicineFudan UniversityShanghai 200032China Department of Pharmacology University of WashingtonSeattleWA 98105United States

BACKGROUND Major depressive disorder(MDD)is a highly disabling psychiatric syndrome associated with deficits of specific subpopulations of cortical GABAergic interneurons;however,the underlying molecular mechanism remains *** 3 adenylyl cyclase(ADCY3,AC3),which is important for neuronal excitability,has been implicated in MDD in a genome-wide association study in ***,a study reported that ablation of AC3 in mice caused similar symptoms as MDD *** To determine if disruption of the AC3 gene in different subtypes of GABAergic interneurons of mice causes depression-like *** Using immunohistochemistry,we investigated the expression of AC3 in two major subtypes GABAergic interneurons:Somatostatin-positive(SST+)and parvalbumin-positive(PV+)*** manipulations were used to selectively disrupt AC3 expression in SST+or PV+interneurons.A series of behavior tests including rotarod test,open field test(OFT),elevated plus maze test(EPM),forced swimming test(FST),and tail suspension test(TST)were used to evaluate the motor ability,anxiety-and depression-like behaviors,*** Our results indicate that approximately 90.41%of SST+and 91.22%of PV+interneurons express *** ablation of AC3 in SST+interneurons,the mice spent comparable time in the center area in OFT,but significantly less time in the open arms and low frequency of entries to the open arms in ***,these mice showed prolonged immobility in FST and more freezing in ***,there were no significant changes in these behaviors after specific disruption of AC3 in PV+*** This study indicates that ablation of AC3 in SST+interneurons of mice increases anxiety-and depression-like behaviors in mice,supporting the general hypothesis that decreased AC3 activity may play a role in human depression.

关键词： Type 3 adenylyl cyclase Somatostatin Parvalbumin Anxiety Depression Mice

rAL Effectors Drive Transcription Bidirectionally in Plants

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Molecular Plant 2017年第2期10卷 285-296页

作者： Li Wang Fabio C. Rinaldi Pallavi Singh Erin L. Doyle Zoe E. Dubrow Tuan Tu Tran Alvaro L. Perez-Quintero Boris Szurek Adam J. Bogdanove Plant Pathology and Plant-Microbe Biology section School of Integrative Plant Science Cornell University 334 Plant Science Building Ithaca NY 14853 USA Department of Biology Doane University 1014 Boswell Avenue Crete NE 68333 USA UMR Interactions-Plantes-Microorganismes-Environnement IRD-Cirad-Universite Montpellier Montpellier France

TAL effectors delivered by phytopathogenic Xanthomonas species are DNA-sequence-specific transcrip- tional activators of host susceptibility genes and sometimes resistance genes. The modularity of DNA recognition by TAL effectors makes them important also as tools for gene targeting and genome editing. Effector binding elements （EBEs） recognized by native TAL effectors in plants have been identified only on the forward strand of target promoters. Here, we demonstrate that TAL effectors can drive plant tran- scription from EBEs on either strand and in both directions. Furthermore, we show that a native TAL effector from Xanthomonas oryzae pv. oryzicola drives expression of a target with an EBE on each strand of its promoter. By inserting that promoter and derivatives between two reporter genes oriented head to head, we show that the TAL effector drives expression from either EBE in the respective orientations, and that activity at the reverse-strand EBE also potentiates forward transcription driven by activity at the forward-strand EBE. Our results reveal new modes of action for TAL effectors, suggesting the possibility of yet unrecognized targets important in plant disease, expanding the search space for off-targets of custom TAL effectors, and highlighting the potential of TAL effectors for probing fundamental aspects of plant transcription.

关键词： xanthomonas transcription activator-like effector bidirectional reporter enhancer element off-target

Long-term administration of scopolamine interferes with nerve cell proliferation, differentiation and migration in adult mouse hippocampal dentate gyrus, but it does not induce cell death

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Neural Regeneration Research 2014年第19期9卷 1731-1739页

作者： Bing Chun Yan Joon Ha Park Bai Hui Chen Jeong-Hwi Cho In Hye Kim Ji Hyeon Ahn Jae-Chul Lee In Koo Hwang Jun Hwi Cho Yun Lyul Lee Il-Jun Kang Moo-Ho Won Department of integrative Traditional & Western Medicine Medical CollegeYangzhou University Department of neurobiology School of MedicineKangwon National University Department of Physiology College of MedicineInstitute of Neurodegeneration and NeuroregenerationHallym University Department of Anatomy and Cell Biology College of Veterinary MedicineSeoul National University Department of Emergency Medicine School of MedicineKangwon National University Department of Food Science and Nutrition Hallym University

Long-term administration of scopolamine, a muscarinic receptor antagonist, can inhibit the survival of newly generated cells, but its effect on the proliferation, differentiation and migration of nerve cells in the adult mouse hippocampal dentate gyrus remain poorly understood. In this study, we used immunohistochemistry and western blot methods to weekly detect the biological behaviors of nerve cells in the hippocampal dentate gyrus of adult mice that received intraperito- neal administration of scopolamine for 4 weeks. Expression of neuronal nuclear antigen （NeuN; a neuronal marker） and Fluoro-]ade B （a marker for the localization of neuronal degeneration） was also detected. After scopolamine treatment, mouse hippocampal neurons did not die, and Ki-67 （a marker for proliferating cells）-immunoreactive cells were reduced in number and reac hed the lowest level at 4 weeks. Doublecortin （DCX; a marker for newly generated neurons）-im- munoreactive cells were gradually shortened in length and reduced in number with time. After scopolamine treatment for 4 weeks, nearly all of the 5-bromo-2＇-deoxyuridine （BrdU）-labeled newly generated cells were located in the subgranular zone of the dentate gyrus, but they did not migrate into the granule cell layer. Few mature BrdU/NeuN double-labeled cells were seen in the subgranular zone of the dentate gyrus. These findings suggest that long-term administration of scopolamine interferes with the proliferation, differentiation and migration of nerve cells in the adult mouse hippocampal dentate gyrus, but it does not induce cell death.

关键词： nerve regeneration neurogenesis scopolamine dentate gyrus cell proliferation neuroblastdifferentiation neuroblast migration granule cell layer neural regeneration