检索结果-南通市图书馆

Overexpression of a Cytosolic Ascorbate Peroxidase Gene, OsAPX2, Increases Salt Tolerance in Transgenic Alfalfa

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2014年第11期13卷 2500-2507页

作者： ZHANG Qian MA Cui XUE Xin XU Ming LI Jing WU Jin-xia biotechnology Research institute Chinese Academy of Agricultural Sciences College of Pastoral agricultural Science and Technology Lanzhou University

Alfalfa （Medicago sativa L.） is an important forage crop in the world and it is of great signiifcance for the improvement of its salt tolerance. To improve salt tolerance in alfalfa, a rice ascorbate peroxidase gene （OsAPX2） was introduced into alfalfa using Agrobacterium tumefaciens-mediated transformation with marker gene bar. The different T-DNA insertions in T1 transgenic alfalfa were identiifed by Southern hybridization. Three independent T2 transgenic lines were selected for stress analysis and the results showed that all of them were salt tolerant compared with wild-type plants. The transgenic plants had low levels of H2O2, malondialdehyde and relative electrical conductivity under salt and drought stresses. Moreover, the contents of chlorophyll and proline, and APX activity were high in transgenic plants under salt and drought stresses. Taken together, the overexpression of OsAPX2 enhances salt tolerance in alfalfa through scavenging reactive oxygen species.

关键词： ascorbate peroxidase (APX) rice (Oryza sativa L.) salt tolerance alfalfa

The miR156/SPL Module, a Regulatory Hub and Versatile Toolbox, Gears up Crops for Enhanced Agronomic Traits

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2015年第5期8卷 677-688页

作者： Hai Wang Haiyang Wang biotechnology Research institute Chinese Academy of Agricultural Sciences Beijing 100081 China

In the past two decades, members of the SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE （SPL） family of transcription factors, first identified in Antirrhinum majus, have emerged as pivotal regulators of diverse biological processes in plants, including the timing of vegetative and reproductive phase change, leaf development, tillering/branching, plastochron, panicle/tassel architecture, fruit ripening, fertility, and response to stresses. Transcripts of a subset of SPLs are targeted for cleavage and/or translational repres- sion by microRNA156s （miR156s）. The levels of miR156s are regulated by both endogenous developmental cues and various external stimuli. Accumulating evidence shows that the regulatory circuit around the miR156/SPL module is highly conserved among phylogenetically distinct plant species, and plays impor- tant roles in regulating plant fitness, biomass, and yield. With the expanding knowledge and a mechanistic understanding of their roles and regulatory relationship, we can now harness the miR156/SPL module as a plethora of tools to genetically manipulate crops for optimal parameters in growth and development, and ultimately to maximize yield by intelligent design of crops.

关键词： agronomic trait crop miR156 SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL)

Effects of lanthanum on abscisic acid regulation of root growth in Arabidopsis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Rare Earths 2014年第1期32卷 78-82页

作者：王建荣王蕾胡婷李文超薛绍武 institute of Molecular Science Shanxi University Key Laboratory of Chemical biology and Molecular Engineering of Ministry of Education Institute of Biotechnology Shanxi University College of Life Science and Technology Huazhong Agricultural University

Rare earth elements （REEs） were reported to have adverse biology effects on plant growth and production. However, whether REEs are involved in plant hormone abscisic acid signal is not clear. Here we reported that REE lanthanum （La） interacted with abscisic acid （ABA） in the regulation of seed germination and root growth in model plant Arabidopsis. La3＋at a concentration of 10 μmol/L alleviated ABA depression of seed germination and reversed ABA inhibition of root elongation growth in Arabidopsis. Previous studies showed that ABA could promote root hair development. In the present study, La3＋inhibited root hair development promoted by ABA. Moreover, La3＋inhibited H2O2 generation induced by ABA in root cells. Therefore we inferred that La3＋might interact with ABA upstream of H2O2 generation.

关键词： lanthanum abscisic acid reactive oxygen species Arabidopsis rare earths

Enhancement of innate immune system in monocot rice by transferring the dicotyledonous elongation factor Tu receptor EFR

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant biology 2015年第7期57卷 641-652页

作者： Fen Lu Huiqin Wang Shanzhi Wang Wendi Jiang Changlin Shan Bin Li Jun Yang Shiyong Zhang Wenxian Sun Department of Plant Pathology China Agricultural University Key Laboratory in Plant Pathology Ministry of AgricultureChina Agricultural University State Key Laboratory of Rice biology and Key Laboratory of Molecular biology of Crop Pathogens and Insects Ministry of AgricultureInstitute of BiotechnologyZhejiang University Rice Research institute Shandong Academy of Agricultural Science

The elongation factor Tu （EF-Tu） receptor （EFR） in cruciferous plants specifically recognizes the N-terminal acetylated elf18 region of bacterial EF-Tu and thereby activates plant immunity. It has been demonstrated that Arabidopsis EFR confers broad-spectrum bacterial resistance in the EFR transgenic solanaceous plants. Here, the transgenic rice plants （Oryza sativa L. ssp. japonica cv. Zhonghua 17） and cell cultures with constitutive expression of AtEFR were developed to investigate whether AtEFR senses EF-Tu and thus enhances bacterial resistance in the monocot plants. We＇demonstrated that the Xanthomonas oryzae-derived elf18 peptide induced oxidative burst and mitogen-activated protein kinase activa- tion in the AtEFR transgenic rice cells and plants, respectively. Pathogenesis-related genes, such as OsPBZ1, were upregulated dramatically in transgenic rice plant and cell lines in response to elf18 stimulation. Importantly, pretreatment with elf18 trig- gered strong resistance to X. oryzae pv. oryzae in the transgenic plants, which was largely dependent on the AtEFR expressionlevel. These plants also exhibited enhanced resistance to rice bacterial brown stripe, but not to rice fungal blast. Collectively, the results indicate that the rice plants with heterologous expression of AtEFR recognize bacterial EF-Tu and exhibit enhanced broad-spectrum bacterial disease resistance and that pattern recognition receptor-mediated immunity may be manipulated across the two plant classes, dicots and monocots.

关键词： Bacterial disease resistance elongation factor Tu receptor interspecific gene transfer pattern recognition receptor rice

Phytochrome Signaling： Time to Tighten up the Loose Ends

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2015年第4期8卷 540-551页

作者： Hai Wang Haiyang Wang biotechnology Research institute Chinese Academy of Agricultural Sciences Beijing 100081 China

Phytochromes are red and far-red light photoreceptors that play fundamental roles in controlling many aspects of plant growth and development in response to light. The past two decades have witnessed the mechanistic elucidation of the action mode of phytochromes, including their regulation by external and endogenous factors and how they exert their function as transcriptional regulators. More importantly, recent advances have substantially deepened our understanding on the integration of the phytochromemediated signal into other cellular and developmental processes, such as elongation of hypocotyls, shoot branching, circadian clock, and flowering time, which ofteninvolves complex intercellular and interorgan signaling. Based on these advances, this review illustrates a blueprint of our current understanding of phytochrome signaling and its crosstalk with other signaling pathways, and also points out still open questions that need to be addressed in the future.

关键词： light regulation light signaling cell signaling photomorphogenesis

The kinesin-like proteins, KAC1/2, regulate actin dynamics underlying chloroplast light-avoidance in Physcomitrella patens

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant biology 2015年第1期57卷 106-119页

作者： Zhiyuan Shen Yen-Chen Liu Jeffrey P.Bibeau Kyle P.Lemoi Erkan Tüzel Luis Vidali Department of biology and biotechnology Worcester Polytechnic Institute Department of Physics Worcester Polytechnic Institute

In plants, light determines chloroplast position; these organelles show avoidance and accumulation re- sponses in high and low fluence-rate light, respectively. Chloroplast motility in response to light is driven by cytoskeletal elements. The actin cytoskeleton mediates chloroplast photorelocation responses in Arabidopsis thali- ana. In contrast, in the moss Physcomitrella patens, both, actin filaments and microtubules can transport chloroplasts. Because of the surprising evidence that two kinesin-like proteins （called KACs） are important for actin-dependent chloroplast photorelocation in vascular plants, we wanted to determine the cytoskeletal system responsible for the function of these proteins in moss. We performed gene- specific silencing using RNA interference in P. patens. We confirmed existing reports using gene knockouts, that PpKAC1 and PpKAC2 are required for chloroplast dispersion under uniform white light conditions, and that the two proteins are functionally equivalent. To address the specificcytoskeletal elements responsible for motility, this loss-of- function approach was combined with cytoskeleton-targeted drug studies. We found that, in P. patens, these KACs mediate the chloroplast light-avoidance response in an actin filament- dependent, rather than a microtubule-dependent manner. Using correlation-decay analysis of cytoskeletal dynamics, we found that PpKAC stabilizes cortical actin filaments, but has no effect on microtubule dynamics.

关键词： Actin filaments cytoskeleton intracellular transport kinesin-14 light-avoidance response microtubule moss RNAi

An integrated pest management program for managing fusarium head blight disease in cereals

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2022年第12期21卷 3434-3444页

作者： CHEN A-hai Tofazzal ISLAM MA Zhong-hua State Key Laboratory of Rice biology/Key Laboratory of Molecular biology of Crop Pathogens and Insects Institute of BiotechnologyZhejiang UniversityHangzhou 310058P.R.China institute of biotechnology and Genetic Engineering(IBGE) Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur 1706Bangladesh

Fusarium head blight(FHB)is a worldwide devastating disease of small grain cereals and Fusarium graminearum species complex(FGSC)is the major pathogen causing the *** epidemics of FHB lead to the reduction of grain yield and economic ***,mycotoxins produced by the FHB pathogens are hazardous to the health of human and *** this review,we summarize the epidemiology of FHB,and introduce effects of this disease on economy,environment and food *** focus on the integrated management approaches for controlling FHB including agronomic practices,resistant cultivars,chemical control,and *** addition,we also discuss the potential novel management strategies against FHB and mycotoxin.

关键词： fusarium head blight Fusarium graminearum integrated pest management program mycotoxins

Genetic variability and evolution of rice stripe virus

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2013年第10期14卷 875-885页

作者： Ling-zhe HUANG Li-xia RAO Xue-ping ZHOU Jian-xiang WU State Key Laboratory of Rice biology Institute of Biotechnology Zhejiang University

Rice stripe virus(RSV) is the type member of the genus *** is known to have four segmented,single-stranded RNA molecules and causes rice stripe disease in the rice fields of China,Japan,and *** on the complete genomic sequences of the determined 6 RSV isolates(from Yunnan,Jiangsu,Zhejiang,and Liaoning Provinces,China) and 27 other RSV isolates(from Yunnan,Jiangsu,Anhui,Henan,and Shandong Provinces of China,also Japan and Korea) downloaded from GenBank,we provided a genotyping profile of RSV field isolates and described the population structure of *** RSV isolates,except isolate CX,could be divided into two subtypes,one including 6 isolates from Yunnan Province,and the other including 26 isolates from different parts of China,Japan,and Korea,which were referred to as subtype II and subtype I,*** amino acid distances between subtypes range from 0.053 to *** isolates in Yunnan Province were genetically differentiated from other parts of China,Japan,and Korea and showed infrequent gene *** RSV populations collected from other parts of China,Japan,and Korea were only composed of subtype I and showed very low genetic *** speculated that isolate CX may be the result of recombination of isolates from two *** potential recombination events were detected in RNA4 of isolate CX.

关键词： Rice stripe virus Genetic variability Genetic evolution

Characterization and Fine Mapping of a Novel Rice Narrow Leaf Mutant nal9

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant biology 2013年第11期55卷 1016-1025页

作者： Wei Li Chao Wu Guocheng Hu Li Xing Wenjing Qian Huamin Si Zongxiu Sun Xingchun Wang Yaping Fu Wenzhen Liu State Key Laboratory of Rice biology China National Rice Research Institute College of Life Sciences Shanxi Agricultural University institute of Horticulture Zhejiang Academy of Agricultural Sciences biotechnology Research Center China Three Gorges University

A narrow leaf mutant was isolated from transgenic rice （Oryza sativa L.） lines carrying a T-DNA insertion. The mutant is characterized by narrow leaves during its whole growth period, and was named nal9 （narrow leaf 9）. The mutant also has other phenotypes, such as light green leaves at the seedling stage, reduced plant height, a small panicle and increased tillering. Genetic analysis revealed that the mutation is controlled by a single recessive gene. A hygromycin resistance assay showed that the mutation was not caused by T-DNA insertion, so a map-based cloning strategy was employed to isolate the nal9 gene. The mutant individuals from the F2 generations of a cross between the nal9mutant and Longtepu were used for mapping. With 24 F2 mutants, the nal9 gene was preliminarily mapped near the marker RM156 on the chromosome 3. New INDEL markers were then designed based on the sequence differences between japonica and indica at the region near RM156. The nal9 gene was finally located in a 69.3 kb region between the markers V239B and V239G within BAC OJ1212_C05 by chromosome walking. Sequence and expression analysis showed that an ATP-dependent CIp protease proteolytic subunit gene （CIpP） was most likely to be the nal9 gene. Furthermore, the nal9 mutation was rescued by transformation of the CIpP cDNA driven by the 35S promoter. Accordingly, the CIpP gene was identified as the NAL9 gene. Our results provide a basis for functional studies of NAL9 in future work.

关键词： ice narrow leaf mutant nal9 fine mapping.