检索结果-南通市图书馆

Ecological immunology:do sexual attraction and immunity trade-off through a desaturase?

在线全文

学校读者我要写书评

暂无评论

Insect Science 2025年第1期32卷 290-300页

作者： Ke Gao Wout van der Heide Daphne Muijderman Sarah Nichols Carmen Karwal Peter Kuperus Astrid T.Groot Institute for Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamthe Netherlands department of Cell and developmental biology University of California San DiegoLa JollaCaliforniaUSA department of Neurobiology and Behavior Cornell UniversityIthacaNew YorkUSA University of Leiden Leidenthe Netherlands

Given the limited availability of resources in nature,sexual attractiveness may trade off with immunocompetence,as the immunocompetence handicap hypothesis(ICHH)*** invertebrates,a direct link between trade-offs through hormonal/molecular effectors in sexual signals and immunity has not been found so ***,we assessed how variation in sexual signals affected parasite infection in two sex pheromone selected lines of the moth Chloridea virescens:an attractive line with a low ratio of 16:Ald/Z11-16:Ald and an unattractive line with a high *** infecting these lines with an apicomplexan parasite,we found that the attractive Low line was significantly more susceptible to the parasite infection than the unattractive High *** the ratio difference between these two lines is determined by a delta-11-desturase,we hypothesized that this desaturase may have a dual role,i.e.,in the quality of the sexual signal as well as an involvement in immune response,comparable to testosterone in ***,when we used CRISPR/cas9 to knockout delta-11-desturase in the attractive Low line,we found that the pheromonal phenotype did change to that of the High line,but the infection susceptibility did ***,when checking the genomic location of delta-11-desaturase in the ***,we found that mucin is adjacent to *** comparing the mucin sequences in both lines,we found four nonsynonymous SNPs in the coding sequence,as well as intronic variation between the two *** differences suggest that genetic hitchhiking may explain the variation in susceptibility to parasitic infection.

关键词： Chloridea virescens delta-11-desaturase ICHH parasite susceptibility sex pheromone

KDM2A and KDM2B protect a subset of CpG islands from DNA methylation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2025年第1期52卷 39-50页

作者： Yuan Liu Ying Liu Yunji Zhu Di Hu Hu Nie Yali Xie Rongrong Sun Jin He Honglian Zhang Falong Lu State Key Laboratory of Molecular developmental biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China department of Biochemistry&Molecular biology College of Natural ScienceMichigan State UniversityEast LansingMI 48824US State Key Laboratory of Animal Nutrition and Feeding Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China

In the mammalian genome,most CpGs are ***,CpGs within the CpG islands(CGIs)are largely unmethylated,which are important for gene expression *** mechanism underlying the low methylation levels at CGIs remains largely ***2 proteins(KDM2A and KDM2B)are H3K36me2 demethylases known to bind specifically at ***,we report that depletion of each or both KDM2 proteins,or mutation of all their JmjC domains that harbor the H3K36me2 demethylation activity,leads to an increase in DNA methylation at selective *** Kdm2a/2b double knockout shows a stronger increase in DNA methylation compared with the single mutant of Kdm2a or Kdm2b,indicating that KDM2A and KDM2B redundantly regulate DNA methylation at *** addition,the increase of CGI DNA methylation upon mutations of KDM2 proteins is associated with the chromatin *** findings reveal that KDM2A and KDM2B function redundantly in regulating DNA methylation at a subset of CGIs in an H3K36me2 demethylation-dependent manner.

关键词： KDM2A KDM2B CpG island DNA methylation H3K36me2 Demethylation Embryonic stemcell

Systematic review of amyloid-beta clearance proteins from the brain to the periphery:implications for Alzheimer's disease diagnosis and therapeutic targets

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第12期20卷 3574-3590页

作者： Letian Huang Mingyue Liu Ze Li Bing Li Jiahe Wang Ke Zhang department of Oncology Shengjing Hospital of China Medical UniversityShenyangLiaoning ProvinceChina department of developmental Cell biology Key Laboratory of Cell BiologyMinistry of Public HealthChina Medical UniversityShenyangLiaoning ProvinceChina department of Family Medicine Shengjing Hospital of China Medical UniversityShenyangLiaoning ProvinceChina

Amyloid-beta clearance plays a key role in the pathogenesis of Alzheimer's disease.H oweve r,the variation in functional proteins involved in amyloid-beta clearance and their correlation with amyloid-beta levels remain unclea *** this study,we conducted meta-analyses and a systematic review using studies from the PubMed,Embase,Web of Science,and Cochrane Library databases,including journal articles published from inception to J une 30,*** inclusion criteria included studies comparing the levels of functional proteins associated with amyloid-beta clearance in the blood,cere b rospinal fluid,and brain of healthy controls,patients with mild cognitive impairment,and patients with Alzheimer's ***,we analyzed the correlation between these functional proteins and amyloid-beta levels in patients with Alzheimer's *** methodological quality of the studies was assessed via the Newcastle-Ottawa *** to heterogeneity,we utilized either a fixed-effect or random-effect model to assess the 95%confidence interval(CI)of the standard mean difference(SMD)among healthy controls,patients with mild cognitive impairment,and patients with Alzheimer's *** findings revealed significant alterations in the levels of insulin-degrading enzymes,neprilysin,matrix metalloproteinase-9,cathepsin D,receptor for advanced glycation end products,and P-glycoprotein in the brains of patients with Alzheimer's disease,patients with mild cognitive impairment,and healthy *** cerebrospinal fluid,the levels of triggering receptor expressed on myeloid cells 2 and ubiquitin C-terminal hydrolase L1 are altered,whereas the levels of TREM2,CD40,CD40L,CD14,CD22,cathepsin D,cystatin C,andα2 M in peripheral blood ***,TREM2 and cathepsin D showed changes in both brain(SMD=0.31,95%CI:0.16-0.47,P<0.001,I^(2)=78.4%;SMD=1.24,95%CI:0.01-2.48,P=0.048,I^(2)=90.1%)and peripheral blood(SMD=1.01,95%CI:0.35-1.66,P=0.003,I^(2)=96.5%;SMD=7.55,95%CI:3.92-11.18,P<0.001,I^(

关键词： Alzheimer’s disease amyloid-β blood‒brain barrier cerebrospinal fluid diagnostic biomarker meta-analysis mild cognitive impairment peripheral blood systematic review therapeutic targets

CSF multi-omics of intracerebral hemorrhage from onset to reperfusion underscores lipid metabolism in functional outcome

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2025年第2期70卷 162-166页

作者： Yibin Jiang Sin Man Lam Shuixian Zhang Huan Miao Yong Zhou Qian Zhang Tengyuan Zhou Hui Feng Ning Ding Haomiao Wang Ran Luo Yi Yin Hua Feng Guanghou Shui Rong Hu department of Neurosurgery and Key Laboratory of Neurotrauma Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China State Key Laboratory of Molecular developmental biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Chongqing Key Laboratory on Big Data for Bio Intelligence Chongqing University of Posts and TelecommunicationsChongqing 400065China Clinical Medical Research Center Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China

Constituting 6.5%to 19.6%of all stroke cases worldwide,non-traumatic intracerebral hemorrhage(ICH)represents the most devastating form of stroke,with aging,male gender,and hypertension being important risk factors that predispose individuals to ICH[1,2].Injuries following episodes of ICH can be classified into two forms,namely(1)primary injury arising from mechanical pressure exerted by the hematoma on adjacent tissues,(2)secondary injury resulting from oxidative stress and inflammation mediated by blood cell degradation products and coagulation factors[3].

关键词： metabolism hemorrhage intracerebral

Compound heterozygous mutation of AFG3L2 causes autosomal recessive spinocerebellar ataxia through mitochondrial impairment and MICU1 mediated Ca2+overload

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2025年第2期68卷 484-501页

作者： Hongyu Li Qingwen Ma Yan Xue Linlin Cai Liwen Bao Lei Hong Yitao Zeng Shu-Zhen Huang Richard H.Finnell Fanyi Zeng Shanghai Institute of Medical Genetics Shanghai Children’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200040China department of Histo-Embryology Genetics and Developmental BiologyShanghai Jiao Tong University School of MedicineShanghai 200025China NHC Key Laboratory of Medical Embryogenesis and developmental Molecular biology Shanghai Key Laboratory of Embryo and Reproduction EngineeringShanghai 200040China Center for Precision Environmental Health Baylor College of MedicineHouston 77030USA School of Pharmacy Macao University of Science and TechnologyMacao 999078China

Autosomal recessive spinocerebellar ataxias(SCARs)are one of the most common neurodegenerative diseases characterized by progressive *** SCARs are known to be caused by mutations in multiple genes,there are still many cases that go undiagnosed or are *** this study,we presented a SCAR patient,and identified a probable novel pathogenic mutation(c.1A>G,p.M1V)in the AFG3L2 start *** proband's genotype included heterozygous mutations of the compound AFG3L2(p.[M1V];[R632X](c.[1A>G];[1894.C>T])),which were inherited from the father(c.1A>G,p.M1V)and mother(c.1894C>T,p.R632X).Functional studies performed on hi PSCs(human induced pluripotent stem cells)generated from the patients and HEK293T cells showed that the mutations impair mitochondrial function and the unbalanced expression of AFG3L2 mRNA and protein ***,this novel mutation resulted in the degradation of the protein and the reduction of the stability of the AFG3L2 protein,and MCU(mitochondrial calcium uniporter)complex mediated Ca2+overload.

关键词： AFG3L2 gene autosomal recessive spinocerebellar ataxia mitochondrial impairment Ca2+overload

Fibrotic liver extracellular matrix induces cancerous phenotype in biomimetic micro-tissues of hepatocellular carcinoma model

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2025年第1期24卷 92-103页

作者： Kosar Nouri Abbas Piryaei Homeyra Seydi Ibrahim Zarkesh Ibrahim Ghoytasi Bahare Shokouhian Mustapha Najimi Massoud Vosough department of developmental biology University of Science and CultureACECR 14155-4364 TehranIran department of Regenerative Medicine Cell Science Research CenterRoyan Institute for Stem Cell Biology and TechnologyACECR 14155-4364 TehranIran department of Stem Cells and developmental biology Cell Science Research CenterRoyan Institute for Stem Cell Biology and TechnologyACECR 14155-4364 TehranIran department of biology and Anatomical Sciences School of MedicineShahid Beheshti University of Medical SciencesTehranIran department of Tissue Engineering and Applied Cell Sciences School of Advanced Technologies in MedicineShahid Beheshti University of Medical SciencesTehranIran department of Cell Engineering Cell Science Research CenterRoyan Institute for Stem Cell Biology and TechnologyACECR 14155-4364 TehranIran department of Medical Biotechnology School of Advanced Technologies in MedicineTehran University of Medical SciencesTehranIran Laboratory of Pediatric Hepatology and Cell Therapy Institute of Experimental and Clinical Research(IREC)UCLouvainBrusselsBelgium Experimental Cancer Medicine Institution for Laboratory MedicineKarolinska InstituteStockholmSweden

Background: Despite considerable advancements in identifying factors contributing to the development of hepatocellular carcinoma(HCC), the pathogenesis of HCC remains unclear. In many cases, HCC is a consequence of prolonged liver fibrosis, resulting in the formation of an intricate premalignant microenvironment. The accumulation of extracellular matrix(ECM) is a hallmark of premalignant microenvironment. Given the critical role of different matrix components in regulating cell phenotype and function, this study aimed to elucidate the interplay between the fibrotic matrix and malignant features in HCC. Methods: Liver tissues from both control(normal) and carbon tetrachloride(CCl_(4))-induced fibrotic rats were decellularized using sodium dodecyl sulfate(SDS) and Triton X-100. The resulting hydrogel from decellularized ECM was processed into micro-particles via the water-in-oil emulsion method. Microparticles were subsequently incorporated into three-dimensional liver biomimetic micro-tissues(MTs) comprising Huh-7 cells, human umbilical vein endothelial cells(HUVECs), and LX-2 cells. The MTs were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) assay at day 11, immunofluorescence staining, immunoblotting, and spheroid migration assay at day 14 after co-culture. Results: Fibrotic matrix from CCl4-treated rat livers significantly enhanced the growth rate of the MTs and their expression of CCND1 as compared to the normal one. Fibrotic matrix, also induced the expression of epithelial-to-mesenchymal transition(EMT)-associated genes such as TWIST1, ACTA2, MMP9, CDH2, and VIMENTIN in the MTs as compared to the normal matrix. Conversely, the expression of CDH1 and hepatic maturation genes HNF4A, ALB, CYP3A4 was decreased in the MTs when the fibrotic matrix was used. Furthermore, the fibrotic matrix increased the migration of the MTs and their secretion of alpha-fetoprotein. Conclusions: Our findings suggest a

关键词： Liver fibrosis Decellularized extracellular matrix Spheroid Epithelial-to-mesenchymal transition Cell differentiation Hepatocellular carcinoma

A PHYB-PIF4-auxin module promotes tomato graft formation in response to elevated ambient temperature

在线全文

学校读者我要写书评

暂无评论

Horticultural Plant Journal 2025年第1期11卷 227-238页

作者： Xianmin Meng Feng Zhang Wencai Yang Qingmao Shang State Key Laboratory of Vegetable Biobreeding Key Laboratory of Horticultural Crop Biology and Germplasm Innovation(Ministry of Agriculture)Institute of Vegetables and FlowersChinese Academy of Agriculture SciencesBeijing 100081China Beijing Key Laboratory of Growth and developmental Regulation for Protected Vegetable Crops Department of Vegetable ScienceCollege of HorticultureChina Agricultural UniversityBeijing 100193China

Grafting is an effective technique for increasing the resistance of vegetables to biotic and abiotic *** has been widely applied to produce solanaceous and melon *** is an important external factor affecting graft ***,the molecular mechanism by which external ambient temperature affects tomato graft formation remains *** this study,we demonstrated that elevating ambient temperature during grafting to 35℃ for more than 24 h after grafting accelerated vascular *** generated self-or heterografted combinations between phyB1B2 and pif4 loss-of-function mutant and wild-type plants,and were mutants unresponsive to graft formation at elevated ambient *** addition,elevated ambient temperature induced SlPIF4 expression during ***4 directly binds the promoters of auxin biosynthesis genes SlYUCCAs and activates their *** investigation revealed auxin accumulation in the graft junction under elevated ambient *** results illuminate the mechanism by which the PHYB-PIF4-auxin module promotes tomato graft formation in response to elevated ambient temperature.

关键词： Graft formation Tomato Temperature Vascular Auxin

Rab1 and Syntaxin 17 regulate hematopoietic homeostasis through β-integrin trafficking in Drosophila

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2025年第1期52卷 51-65页

作者： Fangzhou Luo Luwei Sui Ying Sun Zhixian Lai Chengcheng Zhang Gaoqun Zhang Bing Bi Shichao Yu Li Hua Jin College of Life Sciences Northeast Forestry UniversityHarbinHeilongjiang 150040China department of developmental Immunology Max Planck Institute of Immunobiology and EpigeneticsFreiburgGermany

Hematopoiesis is crucial for organismal health,and Drosophila serves as an effective genetic model due to conserved regulatory mechanisms with *** larvae,hematopoiesis primarily occurs in the lymph gland,which contains distinct zones,including the cortical zone,intermediate zone,medullary zone,and posterior signaling center(PSC).Rab1 is vital for membrane trafficking and maintaining the localization of cell adhesion molecules,yet its role in hematopoietic homeostasis is not fully *** study investigates the effects of Rab1 dysfunction on β-integrin trafficking within circulating hemocytes and lymph gland ***1 impairment disrupts the endosomal trafficking of β-integrin,leading to its abnormal localization on cell membranes,which promotes lamellocyte differentiation and alters progenitor dynamics in circulating hemocytes and lymph glands,*** also show that the mislocalization of β-integrin is dependent on the adhesion protein *** reduction of β-integrin at cell boundaries in PSC cells leads to fewer PSC cells and lamellocyte ***,Rab1 regulates the trafficking of β-integrin via the Q-SNARE protein Syntaxin 17(Syx17).Our findings indicate that Rab1 and Syx17 regulate distinct trafficking pathways for β-integrin in different hematopoietic compartments and maintain hematopoietic homeostasis of Drosophila.

关键词： Hematopoiesis Lymphgland Rab1 Syntaxin 17 β-integrin Drosophila

TRMT6 and TRMT61A facilitated acute pancreatitis severity via regulation of neutrophil function

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Life Sciences 2025年

作者： Yuan Dong Li Su Sin Man Lam Cen Yan Yalan Deng Guotao Lu Jinkui Yang Guanghou Shui Yingmei Feng Beijing Institute of Hepatology Beijing Youan HospitalCapital Medical University department of Science and Development Beijing Youan HospitalCapital Medical University Laboratory for Clinical Medicine Capital Medical University Neuroscience Research Institute Peking University Center of Medical and Health AnalysisPeking University State Key Laboratory of Molecular developmental biology Institute of Genetics and Developmental BiologyChinese Academy of Sciences Pancreatic Center Department of GastroenterologyAffiliated Hospital of Yangzhou UniversityYangzhou University Beijing Key Laboratory of Diabetes Research and Care Department of EndocrinologyBeijing Tongren HospitalCapital Medical University

Dear Editor,Type 2 diabetes mellitus (T2DM) has been identified as a risk factor for increased severity in acute pancreatitis (AP)(Mikóet al,2018).However,to date,no studies have simultaneously included healthy individuals,T2DM patients,and AP patients to investigate which diabetes-associated metabolites are linked to AP severity.

关键词：