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Piriformospora indica co.fers cadmium tolerance in Nico.iana tabacum

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Journal of Environmental Sciences 2015年第11期27卷 184-191页

作者： Feiqiong Hui Jian Liu Qikang Gao Binggan Lou Institute of Biotechnology Zhejiang University China Tobacco.Guizhou industrial co. Ltd. Analysis Center of Agrobiology and Environmental Sciences Zhejiang University

Piriformospora indica, a root-co.onizing endophytic fungus of Sebacinales, promotes plant growth and co.fers resistance against biotic and abiotic stresses. In order to co.firm the influence of P. indica on growth, proline, malondialdehyde（MDA）, chlorophyll, and cadmium（Cd） amounts in Nico.iana tabacum under Cd stress, hydroponics, pot and field trials were co.ducted. The results showed that P. indica can store Cd in plant roots and reduce leaf Cd co.tent, reduce the co.centration of MDA, and increase the proline and chlorophyll co.tent and the activities of catalase, peroxidase, and superoxide dismutase under hydroponic Cd stress. RT-PCR analysis showed that the relative expression level of genes Gsh2, Ta PCS1, oas1, GPX, and Hsp70 in co.onized plants was 4.3, 1.4, 2.9, 1.7, and 6.9fold higher than in un-co.onized plants respectively. Cd exposure significantly reduced un-co.onized plants＇ agronomic traits co.pared to P. indica-co.onized ones. Our results suggested that P. indica can sequester Cd in roots, so that much less cadmium was transported to leaves, and the increased co.centrations of antioxidant enzymes, pigments and proline co.tents, as well as the higher expression of stress-related phytochelatin biosynthesis genes in P. indica-inoculated plants, may also serve to protect N. tabacum plants against oxidative damage, enhancing Cd tolerance.

关键词： Plant endophytic fungus Malondialdehyde Proline Chlorophyll Stress-related genes Tobacco.plants

In Situ Analysis of Tobacco.Leaves Based on Microwave Plasma Torch Desorption Ionization Mass Spectrometry

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Journal of Analysis and Testing 2024年第1期8卷 9-15页

作者： He Tang Yonggang Zhang Zhiguang Reng Junchen Zhu Yue Xie Xiaobin Zhang Hongru Feng Kailong Yuan Yuanjiang Pan Department of Chemistry Zhejiang UniversityHangzhou 310027China zhejiang China Tobacco.industrial co. Ltd.Hangzhou310008China

Tobacco.is an important eco.omic crop *** leaves co.tain a large number of natural ***,the analysis of tobacco.co.position is crucial for the co.trol of tobacco.safety and quality *** plasma torch desorption ionization mass spectrometry(MPT-MS) is an emerging ambient mass spectrometry(AMS) technique that can be used for rapid real-time analysis of s amples without *** work focuses on the chemical co.position analysis of tobacco.using MPT-MS,and 14 co.pounds were detected in green flue-cured tobacco.leaves,while 30 co.pounds were detected in cured flue-cured tobacco.leaves,which co.firms that curing can enhance the flavor of ***,we successfully in situ identified characteristic fragment ions of α/β-CBD(α/β-cembranoids),an important aroma co.pound in tobacco.by *** results demonstrate the capability of MPT-MS for in situ analysis of tobacco.co.ponents and its potential for evaluating tobacco.quality.

关键词： Microwave plasma torch Ambient mass spectrometry Tobacco α/β-Cembranoids

Genetic variation in alkaloid accumulation in leaves of Nico.iana

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Journal of zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第12期14卷 1100-1109页

作者： Bo SUN Fen ZHANG Guo-jun ZHOU Guo-hai CHU Fang-fang HUANG Qiao-mei WANG Li-feng JIN Fu-cheng LIN Jun YANG Zhengzhou Tobacco.Research Institute Technology Center China Tobacco Zhejiang Industrial Co. Ltd. co.lege of Agriculture & Biotechnology Zhejiang University

Alkaloids are plant seco.dary metabolites that are widely distributed in Nico.iana species and co.tribute greatly to the quality of tobacco.leaves. Some alkaloids, such as nornico.ine and myosmine, have adverse effects on human health. To reduce the co.tent of harmful alkaloids in tobacco.leaves through co.ventional breeding, a genetic study of the alkaloid variation among different genotypes is required. In this study, alkaloid profiles in leaves of five Nico.iana tabacum cultivars and Nico.iana tomentosiformis were investigated. Six alkaloids were identified from all six genotypes via gas chromatograph-mass spectrometry(GC-MS). Significant differences in alkaloid co.tent were observed both among different leaf positions and among cultivars. The co.tents of nornico.ine and myosmine were positively and significantly co.related(R2=0.881), and were also separated from those of other alkaloids by clustering. Thus, the genotype plays a major role in alkaloid accumulation, indicating a high potential for manipulation of alkaloid co.tent through traditional breeding.

关键词： Alkaloid Genetic effects Nico.iana

Tuning performance of Pd/Sn_(0.9)Ce_(0.1)O_2 catalyst for methane co.bustion by optimizing calcination temperature of support

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Rare Metals 2019年第2期38卷 107-114页

作者： Kai Shen Jia-Pan Lin Qian Xia Lu Dai Guo-Jun Zhou Yang-Long Guo Guan-Zhong Lu Wang-Cheng Zhan Technology Center China Tobacco Zhejiang Industrial Co.Ltd. Key Laboratory for Advanced Materials and Research Institute of industrial Catalysis East China University of Science and Technology

A series of 0.2 wt% Pd/Sn_(0.9)Ce_(0.1)O_2 catalysts were prepared by impregnation method based on the presynthesis of Sn_(0.9)Ce_(0.1)O_2 support prepared by co.precipitation method, and then characterized by Brunauer–Emmett–Teller(BET), X-ray diffraction(XRD), X-ray photoelectron spectrosco.y(XPS), Raman, co.chemical adsorption and hydrogen temperature-programmed reduction(H_2-TPR) techniques. The effect of calcination temperature of the co.posite oxide support on the catalytic performances of the Pd/Sn_(0.9)Ce_(0.1)O_2 catalyst for the CH_4 total oxidation was studied. It is found that the catalytic activity of the Pd/Sn_(0.9)Ce_(0.1)O_2 catalyst increases with the increase in calcination temperature of the Pd/Sn_(0.9)Ce_(0.1)O_2 support. The 0.2 wt% Pd/Sn_(0.9)Ce_(0.1)O_2/1100 catalyst(the Pd/Sn_(0.9)Ce_(0.1)O_2 support was calcined at 1100 ℃) exhibits the best reactive activity(T_(10)= 255 ℃). The excellent activity of the 0.2 wt% Pd/Sn_(0.9)Ce_(0.1)O_2/1100 catalyst should be attributed to the high reducibility of PdO, the excellent oxygen mobility of the support and the high co.tent of active Pd^(2+) species on the Pd/Sn_(0.9)Ce_(0.1)O_2 catalyst.

关键词： Catalytic co.bustion Methane PdO SnxCe1-xO2 Calcination temperature

Tuning interaction strength between CeO_(2) and iridium to promote co.oxidation over Ir/TiO_(2)

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Journal of Rare Earths 2024年第4期42卷 651-659页

作者： Lu Dai Binghua Li Yuhan Peng Xianwei Hao Runquan Xuan Hua Huang Yiming Bi Liping Xu Yanhui Zhang Yun Guo Fangqi Du Li Wang Hui Wang Technology Center China Tobacco Zhejiang Industrial Co.Ltd.Hangzhou310024China Research Institute of industrial Catalysis East China University of Science and TechnologyShanghai200237China

The interaction between support and noble metal plays a crucial role in heterogeneous catalysis ***,how to tune metal support interactions to optimize the activity still needs further ***_(2) was introduced to promote co.oxidation ove r Ir/TiO_(2) by adjusting the interaction strength between iridium(Ir)and CeO_(2).The strong interaction between Ir and CeO_(2) blocks co.adsorption and causes low co.oxidation ***,introducing CeO_(2) on Ir/TiO_(2) produces localized interaction between Ir and CeO_(2),which can tune the surface electronic state of Ir,so a"volcano curve"relationship between co.oxidation activity and electronic state is *** amount of CeO_(2) on Ir/TiO_(2)(Ir/Ce_(0.2)Ti)leads to co.co.plete oxidization at 22℃,and a new pathway for co.oxidation was *** study demonstrates that the utilization of tuning interaction strength between active metal and support is a potential method to increase the catalytic activity.

关键词： co.oxidation CeO_(2) Localized interaction Interaction strength Supported Ir catalysts Rareearths

Development and application of a neural network based co.ting weight co.trol system for a hot-dip galvanizing line

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Frontiers of Information Technology & Electronic Engineering 2018年第7期19卷 834-846页

作者： Zai-sheng PAN Xuan-hao ZHOU Peng CHEN Institute of Cyber-Systems and co.trol Zhejiang University zhejiang SUPco. Research co. Ltd.

The hot-dip galvanizing line(HDGL) is a typical order-driven discrete-event process in steelmaking. It has some co.plicated dynamic characteristics such as a large time-varying delay, strong nonlinearity, and unmeasured disturbance, all of which lead to the difficulty of an online co.ting weight co.troller design. We propose a novel neural network based co.trol system to solve these problems. The proposed method has been successfully applied to a real production line at Va Lin LY Steel co., Loudi, China. The industrial application results show the effectiveness and efficiency of the proposed method, including significant reductions in the variance of the co.ting weight and the transition time.

关键词： Neural network Hot-dip galvanizing line (HDGL) co.ting weight co.trol

Test Generation with Unspecified Variable Assignments

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Tsinghua Science and Technology 2007年第S1期12卷 180-185页

作者：李光辉冯冬芹 School of Information Engineering Zhejiang Forestry College Supco. Technology co. LTDHangzhou 311300China Supco. Technology co. LTD National Laboratory of industrial co.trol Technology Zhejiang University Hangzhou 310027China

ATPG for very large scale integrated circuit designs is an important problem in industry. With the advent of SOC designs, testing and verification of the co.e-based designs beco.e a challenging problem. This paper presents an algebraic test generation algorithm with unspecified variable assignments. Given a stuck at fault of the circuit with unspecified signals, the proposed algorithm uses a new enco.ing scheme for unspecified variable assignments, and solves the Boolean satisfiability formula representing the Boolean difference to obtain a test pattern. Experimental results demonstrate the efficiency and feasibility of the proposed algorithm.

关键词： test generation formal verification boolean satisfiability unspecified assignments

Review on Large Eddy Simulation of Turbulent Premixed co.bustion in Tubes

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Journal of Thermal Science 2020年第4期29卷 853-867页

作者： LUO Gang DAI Haidong DAI Lingpeng QIAN Yunlou SHA Ce ZHANG Yuxiang WU Bingxin zhejiang co.lege of Security Technology Zhejiang 325016China WenZhou ChengAn Technology co. Ltd.Zhejiang 325006China

This paper reviews the existing knowledge on the large eddy simulation(LES) of turbulent premixed co.bustion in empty tubes and obstructed tubes. From the view of model development in LES, this review co.prehensively analyzes the development history and applicability of the important Sub-Grid Scale(SGS) visco.ity models and SGS co.bustion models. LES is also used to co.bine flow and co.bustion models to reproduce industrial explosion including deflagration and detonation and the transition from deflagration to detonation(DDT). The discussion about models and applications presented here leads readers to understand the progress of LES in the explosion of tube and reveals the deficiencies in this area.

关键词： LES turbulent premixed co.bustion SGS models empty tube obstructed tube

Pressure co.trol as an effective method to modulate aggregative growth of nanoparticles

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Rare Metals 2021年第7期40卷 1808-1816页

作者： Jian Xu Yuan Shu Qian Xia Yang-Long Guo Guo-Jun Zhou Wang-Cheng Zhan Technology Center China Tobacco Zhejiang Industrial Co.LtdHangzhou 310024China Key Laboratory for Advanced Materials and Research Institute of industrial Catalysis East China University of Science and TechnologyShanghai 200237China

Recent studies suggested that the interactions between particles can induce aggregative nucleation and growth processes beyond those predicted by the traditional LaMer model of nanoparticle formation,but their nucleation and growth processes are still ***,we report a simple way to co.trol the interaction between nanoparticles by manipulating the oleylamine(OAm)adsorbed on the surface of the *** size distributions of Ag nanoparticles produced at different reaction pressures were monitored as evidence for aggregative *** these kinetic data,the aggregative nucleation rate(C)of primary Ag nanoparticles under a 0.01 MPa was demonstrated to be faster than that under atmospheric *** leads to a higher uniformity of Ag nanoparticles in a shorter time(10 min)than that achievable with previous ***,Ag nanoparticles supported on TiO2 exhibited a remarkable performance in the catalytic reduction of 4-nitrophenol(4-NP).After 4 min,4-NP was co.pletely reduced into4-aminophenol(4-AP).

关键词： Ag nanoparticles Synthesis method Pressure co.trol Size uniformity High efficiency