Genetic variation in alkaloid accumulation in leaves of Nicotiana

作     者：Bo SUN Fen ZHANG Guo-jun ZHOU Guo-hai CHU Fang-fang HUANG Qiao-mei WANG Li-feng JIN Fu-cheng LIN Jun YANG 

作者机构：Zhengzhou Tobacco Research Institute Technology Center China Tobacco Zhejiang Industrial Co. Ltd. College of Agriculture & Biotechnology Zhejiang University 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2013年第14卷第12期

页      面：1100-1109页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(No.31000137) the China National Tobacco Company and Zhengzhou Tobacco Research Institute Science and Technology Project(No.122009CZ0410) the PhD Programs Foundation of Ministry of Education of China(No.2012M510149) 

主　　题：Alkaloid Genetic effects Nicotiana 

摘      要：Alkaloids are plant secondary metabolites that are widely distributed in Nicotiana species and contribute greatly to the quality of tobacco leaves. Some alkaloids, such as nornicotine and myosmine, have adverse effects on human health. To reduce the content of harmful alkaloids in tobacco leaves through conventional breeding, a genetic study of the alkaloid variation among different genotypes is required. In this study, alkaloid profiles in leaves of five Nicotiana tabacum cultivars and Nicotiana tomentosiformis were investigated. Six alkaloids were identified from all six genotypes via gas chromatograph-mass spectrometry(GC-MS). Significant differences in alkaloid content were observed both among different leaf positions and among cultivars. The contents of nornicotine and myosmine were positively and significantly correlated(R2=0.881), and were also separated from those of other alkaloids by clustering. Thus, the genotype plays a major role in alkaloid accumulation, indicating a high potential for manipulation of alkaloid content through traditional breeding.