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Pedosphere 2024年第2期34卷 424-437页

作者： Wanlin LI Yan XIAO College of Agro-grassland science Nanjing Agricultural UniversityNanjing 210095China

Microplastic pollution is a global and ubiquitous environmental problem in the oceans as well as in the terrestrial *** examined the fate of microplastic polystyrene(MPS)beads in experimental soil in the presence and absence of symbiotic arbuscular mycorrhizal fungi(AMF)and simulated acid rain(SAR)to determine whether the combinations of these three factors altered the growth of white clover Trifolium *** found that MPS,SAR,or AMF added singly to soil did not alter *** growth or *** contrast,MPS and AMF together significantly reduced shoot biomass,while SAR and MPS together significantly reduced soil available phosphorus independent of AMF *** polystyrene,AMF,and SAR together significantly reduced soil NO_(3)^(-)-*** mycorrhizal fungi added singly also enriched the beneficial soil bacteria(genus Solirubrobacter),while MPS combined with AMF significantly enriched the potential plant pathogenic fungus *** mycorrhizal fungi inoculation with MPS increased the abundance of soil hydrocarbon degraders independent of the presence of *** addition,the abundance of soil nitrate reducers was increased by MPS,especially in the presence of AMF and ***,SAR alone increased the abundance of soil pathogens within the fungal community including antibiotic *** findings indicate that the coexistence of MPS,SAR,and AMF may exacerbate the adverse effects of MPS on soil and plant health.

关键词： acid deposition arbuscular mycorrhizal fungi symbiosis hydrocarbon degradation microbial alpha diversity microplastic pollution pathogen

Comparison of Vegetation Indices and Red-edge Parameters for Estimating grassland Cover from Canopy Reflectance Data

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Journal of Integrative Plant Biology 2007年第3期49卷 299-306页

作者： Zhan-Yu Liu Jing-Feng Huang Xin-Hong Wu Yong-Ping Dong Institute of Agriculture Remote Sensing and Information System Application Zhejiang University Hangzhou 310029 China grassland Research Institute Chinese Academy of Agricultural Science Huhhot 010010 China

There has been a great deal of Interests in the estimation of grassland biophysical parameters such as percentage of vegetation cover （PVC）, aboveground biomass, and leaf-area index with remote sensing data at the canopy scale. In this paper, the percentage of vegetation cover was estimated from vegetation indices using Moderate Resolution Imaging Spectroradiometer （MODIS） data and red-edge parameters through the first derivative spectrum from in situ hypserspectral reflectance data. Hyperspectral reflectance measurements were made on grasslands in Inner Mongolia, China, using an Analytical Spectral Devices spectroradiometer. Vegetation indices such as the difference, simple ratio, normalized difference, renormalized difference, soil-adjusted and modified soil-adjusted vegetation indices （DVI, RVI, NDVI, RDVI, SAVI L=0.5 end MSAVI2） were calculated from the hyperspectral reflectance of various vegetation covers. The percentage of vegetation cover was estimated using an unsupervised spectral-contextual classifier automatically. Relationships between percentage of vegetation cover and various vegetation indices and red-edge parameters were compared using a linear and second-order polynomial regression. Our analysis indicated that MSAVI2 and RVI yielded more accurate estimations for a wide range of vegetation cover than other vegetation indices and red-edge parameters for the linear and second-order polynomial regression, respectively.

关键词： grassland hypserspectral remote sensing percentage of vegetation cover red-edge parameter vegetation index

Genetic architecture of leaf morphology revealed by integrated trait module in Catalpa bungei

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Horticulture Research 2023年第4期10卷 161-173页

作者： Miaomiao Zhang Bingyang Liu Yue Fei Xiaowei Yang Linjiao Zhao Chaozhong Shi Yueying Zhang Nan Lu Chuangye Wu Wenjun Ma Junhui Wang State Key Laboratory of Tree Genetics and Breeding Key Laboratory of Tree Breeding and Cultivation of State Forestry AdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing 100091China academy of Forest and grassland Inventory and Planning National Forestry and Grassland AdministrationBeijing 100714China Wenxian Forestry science Research Institute Jiaozuo 454850China

Leaves are crucial for maintaining plant growth and development via photosynthesis,and their function is simultaneously regulated by a suite of phenotypic *** much is known about the genetic architecture of individual leaf traits,unraveling the genetic basis of complex leafmorphology remains a *** on the functional correlation and coordination ofmulti-traits,we divided 15 leaf morphological traits into three modules,comprising size(area,length,width,and perimeter),shape(leaf lobes,aspect ratio,circularity,rectangularity,and the relevant ratios),and color(red,green,and blue)for an ornamental tree species,Catalpa bungei.A total of 189 significant single-nucleotide polymorphisms were identified in the leaves of ***:35,82,and 76 in the size,shape,and color modules,*** quantitative trait loci were common between the size and shape modules,which were closely related according to phenotype correlation,genetic mapping,and mRNA *** color module was independent of *** changes in the aspect ratio,leaf lobe,and circularity suggest that these traits could be the core indicators of the leaf shape *** LAS and SRK genes,associated with leaf lobe and circularity,were found to function in plant defense mechanisms and the growth of *** associations between the SRK and CRK2 genes and the leaf lobe and circularity traits were further verified by *** findings demonstrate the importance of integrating multi-trait modules to characterize leaf morphology and facilitate a holistic understanding of the genetic architecture of intraspecific leaf morphology diversity.

关键词： circular integrating finding

Spatial Heterogeneity of Vegetation in China

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Tsinghua science and Technology 2007年第4期12卷 424-434页

作者：黄大明王昱生 Masae Shiyomi 柳小妮宋百敏陈俊 Shigeo Takahashi Yoshimichi Hori Yasuo Yamamura Ecology Research Station Department of Biological Sciences and Biotechnology Tsinghua University grassland Research Station College of Animal Science and Technology Northeast Agricultural University Faculty of science Ibaraki University Bunkyo 2-1-1 Mito 310-8512 Japan National Institute of Livestock and grassland science 768 Senbonmatsu Nishinasuno Tochigi 329-2793 Japan

The spatial heterogeneity and ecological characteristics of 74 vegetation types and the lack of vegetation type within the 9.6 million km2 of Chinese territory were studied using data from the China vegetation map. The analysis used 877 large quadrats 10 mm×10 mm （actual size 100 km×100 km）, which covered about 8.77 million km^2. Each large-quadrat was divided into four small 5 mm×5mm quadrats （actual size 50 km×50 km）. The frequency of occurrence of all vegetation types was recorded in each small quadrat. The survey using the Shiyomi method of vegetation analysis based on the beta-binomial distribution was adopted to describe the frequency of occurrence and spatial heterogeneity for each kind of vegetation. The weighted average heterogeneity of all the vegetation types in the landscape provides a measure of the landscape level heterogeneity which describes the spatial intricacy of the existing vegetation composition. The maximum spatial vegetation heterogeneity was 0.8620 in the frigid-temperate coniferous forest of Larix （V1）, which dominates cold moist northeast China. The minimum spatial heterogeneity with a low occurrence was the Caragana tibetica in the gravel desert in the western Yellow River Hetao area. The minimum occurrence with a low spatial heterogeneity was the Monsoon rainforest on rock in Guangxi and yunnan Provinces. The vegetation types on the Qing-Zang Plateau were found to be representative of Chinese vegetation because of the very high occurrence and spatial heterogeneity of these vegetation types. The weighted average of the heterogeneity was 0.677, while the vegetation diversity index was 3.29.

关键词： spatial heterogeneity beta-binomial distribution ecosystem evaluation Chinese vegetation

Whole-genome resequencing reveals molecular imprints of anthropogenic and natural selection in wild and domesticated sheep

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Zoological Research 2022年第5期43卷 695-705页

作者： De-Yin Zhang Xiao-Xue Zhang Fa-Di Li Lv-Feng Yuan Xiao-Long Li Yu-Kun Zhang Yuan Zhao Li-Ming Zhao Jiang-Hui Wang Dan Xu Jiang-Bo Cheng Xiao-Bin Yang Wen-Xin Li Chang-Chun Lin Bu-Bo Zhou Wei-Min Wang State Key Laboratory of grassland Agro-Ecosystems Key Laboratory of Grassland Livestock Industry InnovationMinistry of Agriculture and Rural AffairsEngineering Research Center of Grassland IndustryMinistry of EducationCollege of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhouGansu 730020China College of animal science and Technology Gansu Agricultural UniversityLanzhouGansu 730070China Engineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province MinqinGansu 733300China Lanzhou Veterinary Research Institute Chinese Academy of Agricultural SciencesLanzhouGansu 730046China

The abundance of domesticated sheep varieties and phenotypes is largely the result of long-term natural and artificial selection. However, there is limited information regarding the genetic mechanisms underlying phenotypic variation induced by the domestication and improvement of sheep. In this study, to explore genomic diversity and selective regions at the genome level, we sequenced the genomes of 100 sheep across 10 breeds and combined these results with publicly available genomic data from 225 individuals, including improved breeds, Chinese indigenous breeds,African indigenous breeds, and their Asian mouflon ancestor. Based on population structure, the domesticated sheep formed a monophyletic group,while the Chinese indigenous sheep showed a clear geographical distribution trend. Comparative genomic analysis of domestication identified several selective signatures, including IFI44 and IFI44L genes and PANK2 and RNF24 genes, associated with immune response and visual *** genomic analysis of improvement demonstrated that candidate genes of selected regions were mainly associated with pigmentation,energy metabolism, and growth ***, the IFI44 and IFI44L genes showed a common selection signature in the genomes of 30domesticated sheep breeds. The IFI44 c. 54413058C>G mutation was selected for genotyping and population genetic validation. Results showed that the IFI44 polymorphism was significantly associated with partial immune traits. Our findings identified the population genetic basis of domesticated sheep at the whole-genome level, providing theoretical insights into the molecular mechanism underlying breed characteristics and phenotypic changes during sheep domestication and improvement.

关键词： Sheep Whole-genome resequencing Selection signature analysis Immunity IFI44 gene

Linking nutrient strategies with plant size along a grazing gradient： Evidence from Leymus chinensis in a natural pasture

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Journal of Integrative Agriculture 2016年第5期15卷 1132-1144页

作者： LI Xi-liang LIU Zhi-ying REN Wei-bo DING Yong JI Lei GUO Feng-hui HOU Xiang-yang National Forage Improvement Center/Key Laboratory of grassland Resources and Utilization Ministry of Agriculture/Institute of Grassland Research Chinese Academy of Agricultural Sciences Hohhot 010010 P.R.China

St udying the changes in nutrient use strategies induced by grazing can provide insight into the process of grassland degradation and is important for improving grassland quality and enhancing ecosystem function. Dominant species in meadow steppe can optimize their use of limiting resources; however, the regulation of nutrient use strategies across grazing gradients is not fully understood. Therefore, in this study, we report an in situ study in which the impact of grazing rates on nutrient use strategies of Leymus chinensis, the dominant plant species in eastern Eurasian temperate steppes, was investigated. We conducted a large randomized controlled experiment（conducted continuously for five years in grassland plots in a natural pasture in Ha ilar, eastern Mongolia Plateau, China） to assess the effects of grazing rate treatments（0.00, 0.23, 0.34, 0.46, 0.69, and 0.92 adult cattle unit（AU） ha-1） on L. chinensis along a grazing gradient and employed a random sampling approach to compare the accumulation, allocation, and stoichiometry of C, N, and P in leaves and stems. Ou r findings demonstrated the follows：（i） The height of L. chinensis decreased with an increase in the grazing gradient, and the concentrations of C, N, and P significantly increased;（ii） the accumulation of C, N, and P per individual was negatively correlated with the concentration of aboveground tissues, suggesting that there was a tradeoff in L. chinensis between nutrient accumulation and concentration at the individual scale;（iii） the leaf-to-stem ratio of C, N, and P accumulation increased with grazing intensity, indicating a tradeoff in nutrient allocation and plant size at the individual plant level; and（iv） grazing rates were negatively correlated with the ratios of C：N and C：P in the stem; however, these ratios in leaves significantly increased with grazing intensity. Our findings suggest that L. chinensis in meadow steppe adapts to grazing disturbance through tradeoffs between plant size and

关键词： meadow steppe nutrient-use strategy overgrazing stoichiometry Inner Mongolia Leymus chinensis

Confinement and Semi-confinement Raising Fattening Technology of Yaks in Agricultural Areas of Tibet

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animal Husbandry and Feed science 2015年第5期7卷 267-268,283页

作者： Bao Yuhong Feng Ke Qu Guangpeng DunZhuJiaCai Wang Jiu Liao Yangci Shen Muyou Institute of grassland science Tibet Academy of Agricultural and Animal Husbandry Sciences animal Disease Control and Prevention Center of Lhasa

In the study, confinement and semi-confinement raising fattening technology of yaks in agricultural areas of tibet were analyzed. And several suggestions for improving the efficiency of yak raising were put forward. Aiming at providing the reference of healthy raising and sustainable development of yaks in the agricul- tural areas of tibet.

关键词： Agriculture areas of Tibet Yaks Fattening technology Study

Ecological stoichiometry and biomass response of Agropyron michnoi *** simulated N deposition in a sandy grassland,China

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Journal of Arid Land 2020年第5期12卷 741-751页

作者： JIN Xiaoming YANG Xiaogang ZHOU Zhen ZHANG Yingqi YU Liangbin ZHANG Jinghua LIANG Runfang Department of Life science Hulunbuir UniversityHulunbuir 021008China College of Environmental science Northeast Normal UniversityChangchun 130024China grassland Research Institute Chinese Academy of Agricultural SciencesHohhot 010010China

Sandy grassland in northern China is a fragile ecosystem with poor soil *** how plant species regulate growth and nutrient absorption under the background of nitrogen(N)deposition is crucial for the management of the sandy grassland *** carried out a field experiment with six N levels in the Hulunbuir Sandy Land of China from 2014 to 2016 and explored the Agropyron michnoi *** of both aboveground and belowground biomasses and carbon(C),N and phosphorus(P)concentrations in the plant tissues and *** increasing N addition,both aboveground and belowground biomasses and C,N and P concentrations in the plant tissues increased and exhibited a single-peak curve.C:N and C:P ratios of the plant tissues first decreased but then increased,while the trend for N:P ratio was *** peak values of aboveground biomass,belowground biomass and C concentration in the plant tissues occurred at the level of 20 g N/(m2•a),while those of N and P concentrations in the plant tissues occurred at the level of 15 g N/(m2•a).The maximum growth percentages of aboveground and belowground biomasses were 324.2%and 75.9%,respectively,and the root to shoot ratio(RSR)decreased with the addition of N.N and P concentrations in the plant tissues were ranked in the order of leaves>roots>stems,while C concentration was ranked as roots>leaves>*** increase in N concentration in the plant tissues was the largest(from 34%to 162%),followed by the increase in P(from 10%to 33%)and C(from 8%to 24%)*** aboveground biomass was positively and linearly correlated with leaf C,N and P,and soil C and N concentrations,while the belowground biomass was positively and linearly correlated with leaf N and soil C *** results showed that the accumulation of N and P in the leaves caused the increase in the aboveground biomass,while the accumulation of leaf N resulted in the increase in the belowground biomass.N deposition can alter the allocat

关键词： biomass nitrogen deposition plant tissue C,N and P stoichiometry sandy grassland

A preliminary study of water use strategy of desert plants in Dunhuang,China

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Journal of Arid Land 2015年第1期7卷 73-81页

作者： YongQin CUI JianYing MA Wei SUN JiaHuan SUN ZhengHu DUAN Cold and Arid Regions Environmental and Engineering Research Institute Chinese Academy of Sciences Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences Ministry of Education Key Laboratory of Vegetation Ecology Institute of Grassland Science Northeast Normal University

Water is a restrictive factor for plant growth and ecosystem stability in arid and semiarid areas. The dynamics of water availability in soils and water use by plants are consequently critical to ecosystem functions, e.g. maintaining a high resistance to the changing climate. Plant water use strategies, including water-use efficiency （WUE） and the main water source that a plant species utilizes, play an important role in the evaluation of stability and sustainability of a plantation. The water use strategies of desert plants （Tamarix chinensis, Alhagi sparsifolia, Elaeagnus angustifolia, Sophora alopecuroides, Bassia dasyphylla and Nitraria sphaerocarpa） in three different habitats （saline land, sandy land and Gobi） in Dunhuang （located in the typical arid area of northwestern China） were studied. The stable isotope of oxygen was used to determine the main water source and leaf carbon isotope discrimination was used to estimate the long-term WUE of plant species in the summer of 2010. The results suggest that： 1） the studied desert plants took up soil water below the depth of 80 cm; 2） T. chinensis in the three habitats used deeper soil water and T. chinensis in the Gobi site had higher WUE than those in the saline land and the sandy land. The results indicated that desert plants in Dunhuang depended on stable water source and maintained high WUE to survive in water limited environments.

关键词： desert plant stable isotope water source water-use efficiency Dunhuang

Probiotic effect of ferulic acid esterase-producing Lactobacillus plantarum inoculated alfalfa silage on digestion,antioxidant,and immunity status of lactating dairy goats

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animal Nutrition 2022年第4期11卷 38-47页

作者： Fuhou Li Baibing Zhang Yixin Zhang Xia Zhang Samaila Usman Zitong Ding Lizhuang Hao Xusheng Guo State Key Laboratory of grassland Agro-ecosystems School of Life SciencesCollege of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou730000China Probiotics and Biological Feed Research Centre Lanzhou UniversityLanzhou730000China Key Laboratory of Plateau Grazing animal Nutrition and Feed science of Qinghai Province The Academy of Animal and Veterinary SciencesState Key Laboratory of Plateau Ecology and AgricultureQinghai UniversityXining810016China

A feeding experiment was conducted to determine the effects of inoculating alfalfa silage with a ferulic acid esterase-producing inoculum on feed digestibility,rumen fermentation,antioxidant,and immunity status of lactating dairy *** dairy goats were distributed into 2 experimental groups consisting of control diet(Lp MTD/1,including Lactobacillus plantarum MTD/1 inoculated silage)against diet containing silage treated with ferulic acid esterase-producing *** A1(Lp A1).Alfalfa silage inoculated with a ferulic acid esterase-producing Lp A1 had better fermentation quality than the Lp MTD/*** application of Lp A1 improved silage antioxidant capacity as indicated by greater total antioxidant capacity(T-AOC),superoxide dismutase(SOD)and glutathion peroxidase(GSH-Px)activities in Lp A1 treated silage versus Lp MTD/1 *** with Lp MTD/1 treated group,inoculation of silage with Lp A1 increased apparent total tract digestibility of dietary dry matter,organic matter and crude protein,and ruminal concentrations of total volatile fatty acids,acetate,propionate and isobutyrate as *** results of current study also demonstrated improved antioxidant capacity and immune performance of dairy goats with Lp A1 *** Lp A1-treated silage increased dairy goats’serum antioxidase activity,such as T-AOC,SOD,GSH-Px and catalase,and the serum concentration of immunoglobulin A,while decreased tumor necrosis factor a,interleukin(IL)-2 and *** addition,compared with Lp MTD/1,diet containing alfalfa silage inoculated with Lp A1 endowed dairy goats’milk with greater fat and protein contents,improved dairy goat milk quality without affecting feed efficiency.

关键词： Ferulic acid esterase Silage Digestibility Antioxidant Immunity