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世界核心医学期刊文摘（胃肠病学分册） 2005年第7期1卷 49-49页

作者： Abayli B. Colakoglu S. Serin M. 陈瑜 yes ilyurt mah. 19. Sokak. Yonca Sitesi. D Blok Adana Turkey Dr.

Goals: We aimed to investigate the presence of bacterial structures in choles terol gallstones and particularly presence of Helicobacter spp/H. pylori in gall stones by microbiologic cultivation, histopathologic staining, and polymerase ch ain reaction (PCR). Background: Many studies suggest that different mechanisms a re responsible for the formation of pigmented gallstones and cholesterol gallsto nes. Recently, studies showed that infection could have an important role in the formation of cholesterol gallbladder stones. Study: We examined 77 mixed choles terol gallstones. After cholecystectomy, gallbladder cultures were done for H. p ylori and other bacterium. Gallbladder has also been examined by three histopath ologic staining methods (Warthin- Starry, hematoxylin eosin, and gram staining) for Helicobacter spp. In addition, 16S rRNA- PCR amplification was performed f or Helicobacter spp in gallstones. Twenty postmortem gallbladders without gallst ones were investigated by the same histopathologic and PCR methods for Helicobac ter spp. as a control group. Results: Different bacterium were isolated from 22 gallbladder samples (12 Escherichia coli, 8 Pseudomonas, and 2 clostridium) and H. pylori was isolated in 6 gallbladder samples. Helicobacter spp was found in 7 gallstones by PCR amplification. Helicobacter- like organisms were demonstrate d in 18 samples by three different histopathologic methods. Helicobacter- like organisms were also found in five samples by the same histopathologic methods (W arthin- Starry, hematoxylin- eosin, and gram staining). Only four samples were found positive for Helicobacter spp/H. pylori by all methods. Conclusions: Bact erial population including H. pylori could have a possible role in the formation of cholesterol gallstones.

关键词：组织病理学革兰染色色素性微生物培养染色方法假单胞菌形成机制梭菌中起

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