幽门螺杆菌在胆固醇胆结石形成中的作用

作     者：Abayli B. Colakoglu S. Serin M. 陈瑜 

作者机构：Yes ilyurt mah. 19. Sokak. Yonca Sitesi. D Blok Adana Turkey Dr. 

出 版 物：《世界核心医学期刊文摘（胃肠病学分册）》 (Core Journals in Gastroenterology)

年 卷 期：2005年第1卷第7期

页      面：49-49页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：组织病理学 革兰染色 色素性 微生物培养 染色方法 假单胞菌 形成机制 梭菌 中起 

摘      要：Goals: We aimed to investigate the presence of bacterial structures in choles terol gallstones and particularly presence of Helicobacter spp/H. pylori in gall stones by microbiologic cultivation, histopathologic staining, and polymerase ch ain reaction (PCR). Background: Many studies suggest that different mechanisms a re responsible for the formation of pigmented gallstones and cholesterol gallsto nes. Recently, studies showed that infection could have an important role in the formation of cholesterol gallbladder stones. Study: We examined 77 mixed choles terol gallstones. After cholecystectomy, gallbladder cultures were done for H. p ylori and other bacterium. Gallbladder has also been examined by three histopath ologic staining methods (Warthin- Starry, hematoxylin eosin, and gram staining) for Helicobacter spp. In addition, 16S rRNA- PCR amplification was performed f or Helicobacter spp in gallstones. Twenty postmortem gallbladders without gallst ones were investigated by the same histopathologic and PCR methods for Helicobac ter spp. as a control group. Results: Different bacterium were isolated from 22 gallbladder samples (12 Escherichia coli, 8 Pseudomonas, and 2 clostridium) and H. pylori was isolated in 6 gallbladder samples. Helicobacter spp was found in 7 gallstones by PCR amplification. Helicobacter- like organisms were demonstrate d in 18 samples by three different histopathologic methods. Helicobacter- like organisms were also found in five samples by the same histopathologic methods (W arthin- Starry, hematoxylin- eosin, and gram staining). Only four samples were found positive for Helicobacter spp/H. pylori by all methods. Conclusions: Bact erial population including H. pylori could have a possible role in the formation of cholesterol gallstones.