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Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy(PAR-SIM)

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Light(Science & Applications) 2024年第6期13卷 1140-1158页

作者： Xinzhu Xu Wenyi Wang Liang Qiao Yunzhe Fu Xichuan Ge Kun Zhao Karl Zhanghao Meiling Guan Xin Chen Meiqi Li Dayong Jin Peng Xi Department of biomedical engineering College of Future TechnologyPeking UniversityBeijing100871China wallace h.coulter dept.of biomedical engineering Georgia Institute of Technology and Emory UniversityAtlanta30332GAUSA National biomedical Imaging Center Peking UniversityBeijing100871China Department of biomedical engineering College of EngineeringSouthern University of Science and TechnologyShenzhen518055GuangdongChina Airy Technologies Co. Ltd.Beijing100086China Eastern Institute for Advanced Study Eastern Institute of TechnologyNingboZhejiang315200China School of Life Science Peking UniversityBeijing100871China Institute for biomedical Materials and Devices(IBMD) Faculty of ScienceUniversity of Technology SydneySydneyNSW2007Australia

Structured illumination microscopy(SIM)has emerged as a promising super-resolution fluorescence imaging technique,offering diverse configurations and computational strategies to mitigate phototoxicity during real-time imaging of biological *** efforts to enhance system frame rates have concentrated on processing algorithms,like rolling reconstruction or reduced frame reconstruction,or on investments in costly sCMOS cameras with accelerated row readout *** this article,we introduce an approach to elevate SIM frame rates and region of interest(ROI)coverage at the hardware level,without necessitating an upsurge in camera expenses or intricate ***,parallel acquisition-readout SIM(PAR-SIM)achieves the highest imaging speed for fluorescence imaging at currently available detector *** using the full frame-width of the detector through synchronizing the pattern generation and image exposure-readout process,we have achieved a fundamentally stupendous information spatial-temporal flux of 132.9 MPixels·s^(−1),9.6-fold that of the latest techniques,with the lowest SNR of−2.11 dB and 100 nm ***-SIM demonstrates its proficiency in successfully reconstructing diverse cellular organelles in dual excitations,even under conditions of low signal due to ultra-short exposure ***,mitochondrial dynamic tubulation and ongoing membrane fusion processes have been captured in live COS-7 cell,recorded with PAR-SIM at an impressive 408 *** posit that this novel parallel exposure-readout mode not only augments SIM pattern modulation for superior frame rates but also holds the potential to benefit other complex imaging systems with a strategic controlling approach.

关键词： hardware illumination resolution

On-chip physiological mimicry of neurovascular unit: challenges and perspectives

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Neural Regeneration Research 2024年第3期19卷 499-500页

作者： Song Ih Ahn YongTae Kim School of Mechanical engineering Pusan National UniversityBusanSouth Korea George W.Woodruff School of Mechanical engineering Georgia Institute of TechnologyAtlantaGAUSA Parker h.Petit Institute for Bioengineering and Bioscience Georgia Institute of TechnologyAtlantaGAUSA wallace h.coulter Department of biomedical engineering Georgia Institute of TechnologyAtlantaGAUSA Institute for Electronics and Nanotechnology Georgia Institute of TechnologyAtlantaGAUSA

Neurological disorders including neurodegenerative diseases,brain tumors,and stroke are the second leading cause of death and the greatest cause of disability ***,it remains challenging to achieve effective drug delivery to the central nervous system for treatments of neurological diseases due to the blood-brain barrier(BBB).The function of the BBB is regulated by the physiological interactions between various types of cells in the neurovascular unit(NVU).In the NVU,the brain vasculature of the BBB is surrounded by brain pericytes,brain astrocytes,neurons,and microglia(Figure 1).Moreover,the NVU at the levels of arteries and veins includes contractile smooth muscle cells(Schaeffer and Iadecola,2021).

关键词： diseases death arteries

Microgels for Cell Delivery in Tissue engineering and Regenerative Medicine

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Nano-Micro Letters 2024年第10期16卷 576-617页

作者： Leyan Xuan Yingying hou Lu Liang Jialin Wu Kai Fan Liming Lian Jianhua Qiu Yingling Miao hossein Ravanbakhsh Mingen Xu Guosheng Tang Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target and Clinical Pharmacology the NMPA and State Key Laboratory of Respiratory DiseaseSchool of Pharmaceutical Sciences and the Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou 511436People's Republic of China School of Automation Hangzhou Dianzi UniversityHangzhou 310018People's Republic of China Department of biomedical engineering The University of AkronAkronOH 44325USA wallace h.coulter Department of biomedical engineering Georgia Institute of TechnologyAtlantaGA 30332USA

Microgels prepared from natural or synthetic hydrogel materials have aroused extensive attention as multifunctional cells or drug carriers,that are promising for tissue engineering and regenerative *** can also be aggregated into microporous scaffolds,promoting cell infiltration and proliferation for tissue *** review gives an overview of recent developments in the fabrication techniques and applications of microgels.A series of conventional and novel strategies including emulsification,microfluidic,lithography,electrospray,centrifugation,gas-shearing,three-dimensional bioprinting,*** discussed in *** characteristics and applications of microgels and microgel-based scaffolds for cell culture and delivery are elaborated with an emphasis on the advantages of these carriers in cell ***,we expound on the ongoing and foreseeable applications and current limitations of microgels and their aggregate in the field of biomedical *** stimulating innovative ideas,the present review paves new avenues for expanding the application of microgels in cell delivery techniques.

关键词： Microgels Cell delivery Scaffolds 3D bioprinting Single-cell microgels

Advances in Wireless,Batteryless,Implantable Electronics for Real‑Time,Continuous Physiological Monitoring

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Nano-Micro Letters 2024年第3期16卷 254-302页

作者： hyeonseok Kim Bruno Rigo Gabriella Wong Yoon Jae Lee Woon‑hong Yeo IEN Center for Wearable Intelligent Systems and healthcare Georgia Institute of TechnologyAtlantaGA 30332USA George W.Woodruff School of Mechanical engineering Georgia Institute of TechnologyAtlantaGA 30332USA School of Electrical and Computer engineering Georgia Institute of TechnologyAtlantaGA 30332USA wallace h.coulter Department of biomedical engineering Georgia Tech and Emory University School of MedicineAtlantaGA 30332USA Parker h.Petit Institute for Bioengineering and Biosciences Institute for MaterialsInstitute for Robotics and Intelligent MachinesGeorgia Institute of TechnologyAtlantaGA 30332USA

This review summarizes recent progress in developing wireless,batteryless,fully implantable biomedical devices for real-time continuous physiological signal monitoring,focusing on advancing human health *** considerations,such as biological constraints,energy sourcing,and wireless communication,are discussed in achieving the desired performance of the devices and enhanced interface with human *** addition,we review the recent achievements in materials used for developing implantable systems,emphasizing their importance in achieving multi-functionalities,biocompatibility,and *** wireless,batteryless devices offer minimally invasive device insertion to the body,enabling portable health monitoring and advanced disease ***,we summarize the most recent practical applications of advanced implantable devices for human health care,highlighting their potential for immediate commercialization and clinical uses.

关键词： Implantable electronics biomedical systems Batteryless devices Wireless electronics Physiological signal monitoring

Spatial control of robust transgene expression in mouse artery endothelium under ultrasound guidance

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Signal Transduction and Targeted Therapy 2022年第8期7卷 2704-2706页

作者： Renfa Liu Shuai Qu Yunxue Xu hanjoong Jo Zhifei Dai Department of biomedical engineering College of Future TechnologyNational Biomedical Imaging CenterPeking UniversityBeijingChina wallace h.coulter Department of biomedical engineering Georgia Institute of Technology and Emory UniversityAtlantaUSA

Dear Editor,Dysfunctional vascular endothelial cells(ECs)contribute to the pathophysiology of several cardiovascular diseases,such as atherosclerosis and its life-threatening complications.1 Gene therapy can be a valuable approach to modulate endothelial cell function for the prevention of ***,there is still a lack of method for transgene expression in vascular endothelium.2 herein,an effective and specific strategy was established for noninvasive spatial control of transgene expression into the target region of the mouse artery without systemic spillover using an ultrasound and microbubble(MB)guided adenoassociated viral vector(UMGAAV)(Supplementary Fig.S1).To the best of our knowledge,this is the first report on noninvasive spatial control of transgene expression in arterial endothelium in vivo.

关键词： endothelium prevention atherosclerosis

Single-Molecular Co-localization Detection and Analysis

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Single-Molecular Co-localization Detection and Analysis

engineering Novel Molecular Beacon Constructs to Study Intracellular RNA Dynamics and Localization

第十一次中国生物物理学术大会暨第九届全国会员代表大会

作者： Mary D.WANG wallace h.coulter dept.of biomedical engineering Georgia Institute of Technology

＜正＞This talk will discuss single-molecule detection that shows on dual-color optical imaging data,image processing and statistical analysis can reliably differentiate random particle

关键词： single molecule detection co-localization dual-color

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Genomics, Proteomics & Bioinformatics 2017年第5期15卷 279-286页

作者： Zhao Ma Xiaotian Wu Christopher J.Krueger Antony K.Chen Department of biomedical engineering College of Engineering Peking University Beijing 100871 China wallace h.coulter Department of biomedical engineering Georgia Institute of Technology Atlanta GA 30332 USA

With numerous advancements in novel biochemical techniques, our knowledge of the role of RNAs in the regulation of cellular physiology and pathology has grown significantly over the past several decades. Nevertheless, detailed information regarding RNA processing, trafficking, and localization in living cells has been lacking due to technical limitations in imaging single RNA transcripts in living cells with high spatial and temporal resolution. In this review, we discuss tech- niques that have shown great promise for single RNA imaging, followed by highlights in our recent work in the development of molecular beacons （MBs）, a class of nanoscale oligonucleotide-probes, for detecting individual RNA transcripts in living cells. With further refinement of MB design and development of more sophisticated fluorescence microscopy techniques, we envision that MB-based approaches could promote new discoveries of RNA functions and activities.

关键词： Y-O-methyl RNA Phosphorothioate Molecular beacon RNA dynamics Single-molecule RNAimaging

Inhibition of retroviral Gag assembly by non-silencing miRNAs promotes autophagic viral degradation

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Protein & Cell 2018年第7期9卷 640-651页

作者： Na Qu Zhao Ma Mengrao Zhang Muaz N. Rushdi Christopher J. Krueger Antony K. Chen Department of biomedical engineering College of Engineering Peking University Beijing 100871 China wallace h.coulter Department of biomedical-engineering Georgia Institute of Technology Atlanta GA 30332 USA

We recently reported an unconventional mechanism by which miRNAs inhibit hIV-1 viral production. This occurs when miRNAs bind nonspecifically to the viral structural protein Gag, interfering with viral RNA-medi- ated Gag assembly at the plasma membrane. Conse- quently, misassembled viral complexes are redirected into the endocytic pathway where they are delivered to lysosomes for degradation. In this study, we demon- strate that autophagy is a critical mediator of the viral degradation pathway and that this pathway is not hIV-1 specific. Misassembled viral complexes were found to colocalize extensively with LC3 and p62 in late endo- somesllysosomes, demonstrating a convergence of autophagy with functional degradative compartments. Knocking down autophagosome formation machineries reduced this convergence, while treatment with autop- hagy-inducer rapamycin enhanced the convergence. Furthermore, similar autophagy-dependent nonspecific miRNA inhibition of murine leukemia virus （MLV） assembly was shown. Overall, these results reveal autophagy as a crucial regulator of the retroviral degradation pathway in host cells initiated by non- specific miRNA-Gag interactions. These findings could have significant implications for understanding how cells may regulate retroviral complex assembly by miRNA expression and autophagy, and raise the possi- bility that similar regulations can occur in other biolog- ical contexts.

关键词： microRNA Gag protein autophagy

Rational design and synthesis of noble-metal nanoframes for catalytic and photonic applications

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National Science Review 2016年第4期3卷 520-533页

作者： Xue Wang Aleksey Ruditskiy Younan Xia The wallace h.coulter Department of biomedical engineering Georgia Institute of Technology and Emory University State Key Laboratory for Oxo Synthesis and Selective Oxidation Suzhou Research InstituteLanzhou Institute of Chemical PhysicsChinese Academy of Sciences School of Chemistry and Biochemistry Georgia Institute of Technology

Nanoframes are unique for their 3D,highly open *** made of noble metals,they are atractive for use as heterogeneous catalysts because of their large speciic surface areas,high densities of catalytically active sites and low vulnerability toward *** promise to enhance the catalytic activity and durability while reducing the material loading and *** nanoframes composed of Au and/or Ag,they also exhibit highly tunable plasmonic properties similar to those of *** article presents a brief account of recent progress in the design,synthesis and utilization of noble-metal *** start with a discussion of the synthetic strategies,including those involving site-selected deposition and etching,as well as dealloying of both hollow and solid *** then highlight some of the applications enabled by noble-metal ***,we discuss the challenges and trends with regard to future development.

关键词： noble metal nanoframe nanomaterial synthesis catalysis plasmonics