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Nano Research 2018年第6期11卷 3122-3131页

作者： Madeline Vara Younan Xia School of Chemistry and Biochemistry Georgia Institute of Technology Atlanta GA 30132 USA The wallace h. coulter department of biomedical engineering Georgia Institute of Technology and Emory University Atlanta GA 30132 USA

We report a rationally designed one-pot method for the facile synthesis of Pd concave nanocubes in an aqueous solution at room temperature by manipulating the reduction kinetics through the selection of a proper combination of a salt precursor （PdBr4^2-） and reductant （sodium ascorbate）. Our kinetic analysis demonstrates that, through this selection, the nucleation and growth of Pd nanocrystals could be effectively separated into two kinetic regimes involving distinctive reduction pathways： i） solution reduction for the initial formation of single-crystal seeds and ii） surface reduction for the formation of concave nanocrystals via autocatalytic growth from the single-crystal seeds. The suppressed surface diffusion at room temperature, when coupled with the capping effect of bromide ions, ultimately leads to the formation of concave nanocubes with an asymmetric shape and high-index facets, whose synthesis would otherwise require multiple steps and the use of elevated temperatures.

关键词： Pd concave cubes growth kinetics nanocrystal synthesis

Deep UV Microscopy Identifies Prostatic Basal Cells:An Important Biomarker for Prostate Cancer Diagnostics

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biomedical engineering Frontiers 2022年第1期3卷 407-419页

作者： Soheil Soltani Brian Cheng Adeboye O.Osunkoya Francisco E.Robles wallace h.coulter department of biomedical engineering Georgia Institute of Technology and Emory UniversityAtlantaGA 30332USA departments of Pathology and Urology Emory University School of MedicineAtlantaGA 30322USA Winship Cancer Institute of Emory University AtlantaGA 30322USA

Objective and Impact *** benign mimics of prostatic adenocarcinoma remains a significant diagnostic *** this work,we developed an approach based on label-free,high-resolution molecular imaging with multispectral deep ultraviolet(UV)microscopy which identifies important prostate tissue components,including basal *** work has significant implications towards improving the pathologic assessment and diagnosis of prostate *** of the most important indicators of prostate cancer is the absence of basal cells in glands and ***,identifying basal cells using hematoxylin and eosin(h&E)stains,which is the standard of care,can be difficult in a subset of *** such situations,pathologists often resort to immunohistochemical(IhC)stains for a definitive ***,IhC is expensive and time-consuming and requires more tissue sections which may not be *** addition,IhC is subject to false-negative or false-positive stains which can potentially lead to an incorrect *** leverage the rich molecular information of label-free multispectral deep UV microscopy to uniquely identify basal cells,luminal cells,and inflammatory *** method applies an unsupervised geometrical representation of principal component analysis to separate the various components of prostate tissue leading to multiple image representations of the molecular *** results show that this method accurately and efficiently identifies benign and malignant glands with high fidelity,free of any staining procedures,based on the presence or absence of basal *** further use the molecular information to directly generate a high-resolution virtual IhC stain that clearly identifies basal cells,even in cases where IhC stains *** simple,low-cost,and label-free deep UV method has the potential to improve and facilitate prostate cancer diagnosis by enabling robust identification of basal cells an

关键词： diagnosis false resort

Quantitative Analysis of the Reduction Kinetics of a Pt(Ⅱ) Precursor in the Context of Pt Nanocrystal Synthesis

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Chinese Journal of Chemical Physics 2018年第4期31卷 370-374,613页

作者： Shan Zhou Tung-hanYang MingZhao YounanXia School of Chemistry and Biochemistry Georgia Institute of TechnologyAtlantaGeorgia 30332USA The wallace h.coulter department of biomedical engineering Georgia Institute of Technology and Emory UniversityAtlantaGeorgia 30332USA

In this letter, we report a quantitative analysis of how a Pt（II） precursor is reduced to atoms at different temperatures for the formation of Pt nanocrystals with different morphologies and sizes. Our results suggest that in the early stage of a synthesis, the Pt（II） precursor is reduced to atoms exclusively in the solution phase, followed by homogeneous nucleation to generate nuclei and then seeds. At a relatively low reaction temperature such as 22℃, the growth of the seeds is dominated by autocatalytic surface reduction that involves the adsorption and then reduction of the Pt（II） precursor on the surface of the just-formed seeds. This particular growth pathway results in relatively large assemblies of Pt nanocrystals. When the reaction temperature is increased to 100 ℃, the dominant reduction pathway will be switched from surface to solution phase, producing much smaller asselnblies of Pt nanocrystals. Our results also demonstrate that a similar trend applies to the seed-rnediated growth of Pt nanocrystals in the presence of Pd nanocubes.

关键词： Kinetic model Nanocrystal synthesis Precursor reduction

Single-Molecular Co-localization Detection and Analysis

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Single-Molecular Co-localization Detection and Analysis

Molecular Biomechanics

第十一次中国生物物理学术大会暨第九届全国会员代表大会

作者： Mary D.WANG wallace h.coulter Dept.of biomedical engineering Georgia Institute of Technology

＜正＞This talk will discuss single-molecule detection that shows on dual-color optical imaging data,image processing and statistical analysis can reliably differentiate random particle

关键词： single molecule detection co-localization dual-color

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Molecular Biomechanics

Gold nanocages as multifunctional materials for nanomedicine

中国力学大会——2013

作者： Cheng Zhu wallace h.coulter department of biomedical engineering Georgia Institute of Technology and Emory UniversityUSA

Molecular biomechanics includes two interconnected themes:The first is the study of mechanical aspects of biomolecules,including but not limited to molecular deformation,conformational change,protein unfolding,and force-regulated molecular *** second is the study of molecular biology of the cell using mechanical tools, methods,and *** two themes are closely related for obvious reasons.A major objective of studying the

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Frontiers of physics 2014年第3期9卷 378-384页

作者： Xiaohu Xia The wallace h. coulter department of biomedical engineering Georgia Institute of Technology and Emory University Medical School Atlanta GA 30332 USA School of Chemistry and Biochemistry School of Chemical and Biomolecular Engineering Georgia Institute of Technology Atlanta GA 30332 USA

Featured by tunable localized surface plasmon resonance peaks in the near-infrared region and hollow interiors, Au nanocages represent a novel class of multifunctional nanomat, erials that have gained considerable attention in recent vears. This short review summarizes our recent work on the capabilities of An nanocages in nanomedicine. We start with a brief description of the synthesis of Au nanocages and highlight our recent protocols for the scaled-up production of An ***. We then use a numer of examples to illustrate how Au nanocages can contribute to nanomedicine with respect to both diagnosis and therapy.

关键词： gold nanocage nanomedicine diagnosis therapy

Microgels for Cell Delivery in Tissue engineering and Regenerative Medicine

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Nano-Micro Letters 2024年第10期16卷 576-617页

作者： Leyan Xuan Yingying hou Lu Liang Jialin Wu Kai Fan Liming Lian Jianhua Qiu Yingling Miao hossein Ravanbakhsh Mingen Xu Guosheng Tang Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target and Clinical Pharmacology the NMPA and State Key Laboratory of Respiratory DiseaseSchool of Pharmaceutical Sciences and the Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou 511436People's Republic of China School of Automation Hangzhou Dianzi UniversityHangzhou 310018People's Republic of China department of biomedical engineering The University of AkronAkronOH 44325USA wallace h.coulter department of biomedical engineering Georgia Institute of TechnologyAtlantaGA 30332USA

Microgels prepared from natural or synthetic hydrogel materials have aroused extensive attention as multifunctional cells or drug carriers,that are promising for tissue engineering and regenerative *** can also be aggregated into microporous scaffolds,promoting cell infiltration and proliferation for tissue *** review gives an overview of recent developments in the fabrication techniques and applications of microgels.A series of conventional and novel strategies including emulsification,microfluidic,lithography,electrospray,centrifugation,gas-shearing,three-dimensional bioprinting,*** discussed in *** characteristics and applications of microgels and microgel-based scaffolds for cell culture and delivery are elaborated with an emphasis on the advantages of these carriers in cell ***,we expound on the ongoing and foreseeable applications and current limitations of microgels and their aggregate in the field of biomedical *** stimulating innovative ideas,the present review paves new avenues for expanding the application of microgels in cell delivery techniques.

关键词： Microgels Cell delivery Scaffolds 3D bioprinting Single-cell microgels

Bi-FoRe:an efficient bidirectional knockin strategy to generate pairwise conditional alleles with fluorescent indicators

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Protein & Cell 2021年第1期12卷 39-56页

作者： Bingzhou han Yage Zhang Xuetong Bi Yang Zhou Christopher JKrueger Xinli hu Zuoyan Zhu Xiangjun Tong Bo Zhang Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education Peking University Genome Editing Research CenterCollege of Life SciencesPeking UniversityBeiing 100871China School of Biology and Biological engineering South China University of TechnologyGuangzhou 510006China department of biomedical engineering College of EngineeringPeking UniversityBeijing 100871China wallace h.coulter department of biomedical engineering Georgia Institute of Techno logy and EmoryAtlantaGA 33032USA Institute of Molecular Medicine Peking UniversityBejing 100871China

Gene expression labeling and conditional manipulation of gene function are important for elaborate dissection of gene ***,contemporary generation of pairwise dual-function knockin alleles to achieve both conditional and geno-tagging effects with a single donor has not been *** we first developed a strategy based on a flipping donor named FoRe to generate conditional knockout alleles coupled with fluorescent allele-labeling through NhEJ-mediated unidirectional targeted insertion in zebrafish facilitated by the CRISPR/Cas *** demonstrated the feasibility of this strategy at sox10 and isl1 loci,and successfully achieved Cre-induced conditional knockout of target gene function and simultaneous switch of the fluorescent reporter,allowing generation of genetic mosaics for lineage *** then improved the donor design enabling efficient one-step bidirectional knockin to generate paired positive and negative conditional alleles,both tagged with two different fluorescent *** introducing Cre recombinase,these alleles could be used to achieve both conditional knockout and conditional gene restoration in parallel;furthermore,differential fluorescent labeling of the positive and negative alleles enables simple,early and efficient realtime discrimination of individual live embryos bearing different genotypes prior to the emergence of morphologically visible *** named our improved donor as Bi-FoRe and demonstrated its feasibility at the sox10 ***,we eliminated the undesirable bacterial backbone in the donor using minicircle DNA *** system could easily be expanded for other applications or to other organisms,and coupling fluorescent labeling of gene expression and conditional manipulation of gene function will provide unique opportunities to fully reveal the power of emerging single-cell sequencing technologies.

关键词： CRISPR/Cas conditional knockout allele labeling conditional rescue minicircle DNA

Two-photon MINFLUX with doubled localization precision

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eLight 2022年第1期2卷 58-67页

作者： Kun Zhao Xinzhu Xu Wei Ren Dayong Jin Peng Xi department of biomedical engineering College of Future TechnologyPeking UniversityBeijing 100871China wallace h.coulter department of biomedical engineering Georgia Institute of Technology and Emory UniversityAtlanta30332 GeorgiaUSA UTS‑SUStech Joint Research Centre for biomedical Materials&Devices Department of Biomedical EngineeringCollege of EngineeringSouthern University of Science and TechnologyShenzhenGuangdong 518055China National biomedical Imaging Center Peking UniversityBeijing 100871China

Achieving localization with molecular precision has been of great interest for extending fluorescence microscopy to *** pioneers this transition through point spread function(PSF)engineering,yet its performance is primarily limited by the signal-to-background *** we demonstrate theoretically that two-photon MINFLUX(2p-MINFLUX)could double its localization precision through PSF engineering by nonlinear ***ér-Rao Bound(CRB)is studied as the maximum localization precision,and CRB of two-photon MINFLUX is halved compared to single-photon MINFLUX(1p-MINFLUX)in all three ***,in order to achieve same localization precision with 1p-MINFLUX,2p-MINFLUX requires only 1/4 of fluorescence *** simultaneous two-photon excitation of multiple fluorophore species,2p-MINFLUX may have the potential for registration-free nanoscopy and multicolor tracking.

关键词： Super-resolution microscopy Single-molecule localization MINFLUX Two-photon microscopy

On-chip physiological mimicry of neurovascular unit: challenges and perspectives

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Neural Regeneration Research 2024年第3期19卷 499-500页

作者： Song Ih Ahn YongTae Kim School of Mechanical engineering Pusan National UniversityBusanSouth Korea George W.Woodruff School of Mechanical engineering Georgia Institute of TechnologyAtlantaGAUSA Parker h.Petit Institute for Bioengineering and Bioscience Georgia Institute of TechnologyAtlantaGAUSA wallace h.coulter department of biomedical engineering Georgia Institute of TechnologyAtlantaGAUSA Institute for Electronics and Nanotechnology Georgia Institute of TechnologyAtlantaGAUSA

Neurological disorders including neurodegenerative diseases,brain tumors,and stroke are the second leading cause of death and the greatest cause of disability ***,it remains challenging to achieve effective drug delivery to the central nervous system for treatments of neurological diseases due to the blood-brain barrier(BBB).The function of the BBB is regulated by the physiological interactions between various types of cells in the neurovascular unit(NVU).In the NVU,the brain vasculature of the BBB is surrounded by brain pericytes,brain astrocytes,neurons,and microglia(Figure 1).Moreover,the NVU at the levels of arteries and veins includes contractile smooth muscle cells(Schaeffer and Iadecola,2021).

关键词： diseases death arteries