检索结果-南通市图书馆

Microglia lactylation in relation to central nervous system diseases

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第1期20卷 29-40页

作者： Hui Yang Nan Mo Le Tong Jianhong Dong Ziwei Fan Mengxian Jia Juanqing Yue Ying Wang School of Pharmacy Hangzhou Normal UniversityHangzhouZhejiang ProvinceChina Department of Clinical Laboratory The Fourth Clinical Medical College of Zhejiang University of Traditional Chinese Medicine(Hangzhou First People’s Hospital)HangzhouZhejiang ProvinceChina College of medical Veterinary and Life SciencesUniversity of GlasgowGlasgowUK Department of Orthopedics(Spine Surgery) the First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina Department of Pathology Affiliated Hangzhou First People’s HospitalWestlake University School of MedicineHangzhouZhejiang ProvinceChina Department of Clinical Research Center Affiliated Hangzhou First People’s HospitalWestlake University School of MedicineHangzhouZhejiang ProvinceChina

The development of neurodegenerative diseases is closely related to the disruption of central nervous system ***,as innate immune cells,play important roles in the maintenance of central nervous system homeostasis,injury response,and neurodegenerative *** has been considered a metabolic waste product,but recent studies are revealing ever more of the physiological functions of *** is an important pathway in lactate function and is involved in glycolysis-related functions,macrophage polarization,neuromodulation,and angiogenesis and has also been implicated in the development of various *** review provides an overview of the lactate metabolic and homeostatic regulatory processes involved in microglia lactylation,histone versus non-histone lactylation,and therapeutic approaches targeting ***,we summarize the current research on microglia lactylation in central nervous system diseases.A deeper understanding of the metabolic regulatory mechanisms of microglia lactylation will provide more options for the treatment of central nervous system diseases.

关键词： brain central nervous system glycolysis immune response inflammation lactate metabolism lactate lactylation microglia neurodegenerative diseases

From mice to humans:a need for comparable results in mammalian neuroplasticity

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第2期20卷 464-466页

作者： Marco Ghibaudi Enrica Boda Luca Bonfanti Neuroscience Institute Cavalieri Ottolenghi OrbassanoItaly Department of veterinary Sciences University of TurinTurinItaly Department of Neuroscience Rita Levi-Montalcini University of TurinTurinItaly

Brain plasticity-A universal tool with many variations:The study of brain plasticity has been gaining interest since almost a century and has now reached a huge amount of information(>80,000 results in PubMed).Overall,different types of plasticity,including stem cell-driven genesis of new neurons(adult neurogenesis),cells in arrested maturation(dormant neurons),neuro-glial and synaptic plasticity,can coexist and contribute to grant plastic changes in the brain,from a cellular to system level(Benedetti and Couillard-Despres,2022;Bonfanti et al.,2023).

关键词： plasticity al. arrest

A Semi-quantitative Serological Method to Assess the Potency of Inactivated Rabies Vaccine for veterinary Use

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2012年第4期27卷 259-264页

作者： Ye Liu Shoufeng Zhang Fei Zhang Rongliang Hu Laboratory of Epidemiology Military Veterinary Research InstituteAcademy of Military Medical SciencesChangchun 130122China

Potency is one of the most important indexes of inactivated vaccines.A number of methods have been established to assay the potency,of which the NIH test and single-dose mouse protection test are the "prescribed methods".Here,we report a method to semi-quantitatively assay the potency of an inactivated rabies vaccine,which uses fewer animals and takes less time to *** on the quality requirements of a vaccine(*** potency),a rabies reference vaccine is,for example,diluted to the minimum potency,and 50 μL of the dilution is taken to inoculate 10 *** same amount of the test rabies vaccine is inoculated into another 10 *** two weeks,all mice are bled and serum samples are assayed for viral neutralizing antibody by the fluorescent antibody virus neutralization(FAVN) *** comparing the median and interquartile range of antibody titers of the reference vaccine with those of the test vaccine,the test vaccine potency can be semi-quantitatively judged as to whether it is in accord with the required *** reliability of this method was also confirmed in *** procedure can be recommended for batch potency testing during inactivated rabies vaccine production.

关键词： Rabies Inactivated vaccine Potency assay Semi-quantitative method

In vitro antiproliferative and apoptosis inducing effect of a methanolic extract of Azadirachta indica oil on selected cancerous and noncancerous cell lines

在线全文

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2018年第10期11卷 555-561页

作者： Muhammad Kashif Dongwook Kim Gonhyung Kim veterinary medical Center College of Veterinary Medicine Chungbuk National University

Objective: To find the cytotoxic and apoptotic effects of neem oil extract on the selected cancerous(A-549, PC-3 and DU-145) and noncancerous(NIH3T3 and CCD-18Co) cell ***: Viability and cytotoxic effect induced by the extract was measured by using MTT assay and apoptotic effect of the extract was evaluated by using Hoechst 33342 and propidium iodide dual staining through a fluorescent microscope and activity of caspases 3, 8 and 9 through colorimetric assay kits. Results: The results showed that neem oil extract significantly reduced the viability in all selected cancer cells treated with varying concentrations of extract as compared with untreated cells and had less effect on noncancerous cell lines. It significantly increased the percentage of necrotic and apoptotic cells, and caspases 3, 8 and 9 activities in all cancer cells treated with extract as compared with untreated cells whereas no effect on noncancerous cell lines. It suggested that neem oil extract exerted a higher cytotoxic effect on cancer cells than normal cells and lower concentration induced apoptosis only in cancer *** of the apoptosis-inducing mechanism was through the activation of caspases signaling pathways. Conclusion: Conclusively, it implies that neem oil extract may contain one or more potential agents that can be used as a safe and effective anticancer therapy.

关键词： Neem oil extract Cell lines Cytotoxicity Apoptosis Caspase activity

Acute phase reactants, challenge in the near future of animal production and veterinary medicine

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2005年第10期6卷 941-947页

作者： GRUYS E. ,TOUSSAINT M.J.M. ,UPRAGARIN N. ,VAN EDEREN A.M. ,ADEWUYI A.A. ,CANDIANI D. ,NGUYEN T.K.A. ,SABECKIENE (BALCIUTE) J. Department of Pathobiology Faculty of veterinary Medicine Utrecht University Utrecht the Netherlands Department of Pathobiology Faculty of veterinary Medicine Utrecht University Utrecht the Netherlands Department of Pathobiology Faculty of veterinary Medicine Utrecht University Utrecht the Netherlands Department of Pathobiology Faculty of veterinary Medicine Utrecht University Utrecht the Netherlands Department of Pathobiology Faculty of veterinary Medicine Utrecht University Utrecht the Netherlands Department of Pathobiology Faculty of veterinary Medicine Utrecht University Utrecht the Netherlands Department of Pathobiology Faculty of veterinary Medicine Utrecht University Utrecht the Netherlands Department of Pathobiology Faculty of veterinary Medicine Utrecht University Utrecht the Netherlands Department of Farm Resources and Production Medicine Faculty of veterinary Medicine Kasetsart University Nakhon Pathom Thailand Department of veterinary Morphophysiology University of Torino Italy Department of Embryo Transfer and Reproduction the National Institute of Animal Husbandry Hanoi Vietnam Department of Infectious Diseases Lithuanian veterinary Academy Kaunas Lithuania)

The future of acute phase proteins (APPs) in science is discussed in this paper. Many functions and associated pathological processes of APPs are unknown. Extrahepatic formation in local tissues needs attention. Local serum amyloid A (SAA) formation may be involved in deposition of AA-amyloid induced by conformational change of SAA resulting in amyloid formation, having tremendous food safety implications. Amyloidogenesis is enhanced in mouse fed beta pleated sheet-rich pro- teins. The local amyloid in joints of chicken and mammary corpora amylacea is discussed. Differences in glycosylation of glycoproteins among the APPs, as has been shown for α1-acid glycoprotein, have to be considered. More knowledge on the reactivity patterns may lead to implication of APPs in the diagnostics and staging of a disease. Calculation of an index from values of several acute phase variables increases the power of APPs in monitoring unhealthy individuals in animal populations. Vaccinations, just as infections in eliciting acute phase response seem to limit the profitability of vaccines because acute phase reactions are contraproductive in view of muscle anabolism. Interest is focused on amino acid patterns and vitamins in view of dietary nutrition effect on sick and convalescing animals. When inexpensive methodology such as liquid phase methods (nephelometry, turbidimetry) or protein array technology for rapid APPs measurement is available, APPs have a future in routine diagnostics. Specific groups of patients may be screened or populations monitored by using APPs.

关键词： Acute phase protein Amino acid Joint Mammary gland Mastitis Serum amyloid A (SAA) Vitamin A

Temporal profiles of synaptic plasticity-related signals in adult mouse hippocampus with methotrexate treatment

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2012年第21期7卷 1651-1658页

作者： Miyoung Yang Juhwan Kim Sung-Ho Kim Joong-Sun Kim Taekyun Shin Changjong Moon Department of veterinary Anatomy College of Veterinary Medicine and Animal Medical Institute Chonnam National University Department of Experimental Radiation Research Center Dongnam Institute of Radiological & Medical Sciences (DIRAMS) Department of veterinary Anatomy College of Veterinary Medicine Jeju National University

Methotrexate, which is used to treat many malignancies and autoimmune diseases, affects brain functions including hippocampal-dependent memory function. However, the precise mechanisms underlying methotrexate-induced hippocampal dysfunction are poorly understood. To evaluate temporal changes in synaptic plasticity-related signals, the expression and activity of N-methyI-D-aspartic acid receptor 1, calcium/calmodulin-dependent protein kinase II, extracellular signal-regulated kinase 1/2, cAMP responsive element-binding protein, glutamate receptor 1, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor were examined in the hippocampi of adult C57BL/6 mice after methotrexate （40 mg/kg） intraperitoneal injection. Western blot analysis showed biphasic changes in synaptic plasticity-related signals in adult hippocampi following methotrexate treatment. N-methyI-D-aspartic acid receptor 1, calcium/calmodulin-dependent protein kinase II, and glutamate receptor 1 were acutely activated during the early phase （1 day post-injection）, while extracellular signal-regulated kinase 1/2 and cAMP responsive element-binding protein activation showed biphasic increases during the eady （1 day post-injection） and late phases （7-14 days post-injection）. Brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor expression increased significantly during the late phase （7-14 days post-injection）. Therefore, methotrexate treatment affects synaptic plasticity-related signals in the adult mouse hippocampus, suggesting that changes in synaptic plasticity-related signals may be associated with neuronal survival and plasticity-related cellular remodeling.

关键词： hippocampus methotrexate neurotrophic factor synaptic plasticity-related signal neuralregeneration

New insight in a new entity:the mitochondrial permeability transition pore arises from the Ca^(2+) -activated F_1F_O-ATPases

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2018年第3期63卷 143-145页

作者： Salvatore Nesci Department of veterinary medical Sciences (DIMEVET) University of Bologna

The mitochondrial permeability transition pore(MPTP)is linked to pathways leading to different variant of cell death and the molecular mechanism of MPTP opening is considered a promising approach from structure to med... 详细信息

关键词：毛孔渗透实体 Ca MPTP

Baicalin inhibits colistin sulfate-induced apoptosis of PC12 cells

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2013年第28期8卷 2597-2604页

作者： Hong Jiang Pengfei Lv Jichang Li Hongjun Wang Tiezhong Zhou Yingzi Liu Wei Lin Institute of veterinary Medicine Northeast Agricultural University Institute of Animal Husbandry and veterinary Medicine Liaoning Medical University Scientific Research Department Liaoning Medical Universit

Baicalin, a type of flavonoid extracted from the dried root of Scutellaria baicalensis georgi, has been shown to effectively inhibit cell apoptosis. Therefore, we assumed that baicalin would suppress colistin sulfate-induced neuronal apoptosis. PC12 cells exposed to colistin sulfate （62.5-500 μg/mL） for 24 hours resulted in PCl2 cell apoptosis. In addition, caspase-3 activity, lactate dehydrogenase level and free radical content increased in a dose-dependent manner. Subsequently, PC12 cells were pretreated with baicalin （25, 50 and 100 pg/mL）, and exposed to 125 pg/mL colistin sulfate. Cell morphology markedly changed, and cell viability increased. Moreover, caspase-3 activity, lac- tate dehydrogenase level and free radical content decreased. Results indicated that baicalin inhib- ited colistin sulfate-induced PC12 cell apoptosis by suppressing free radical injury, and reducing caspase-3 activity and lactate dehydrogenase activity.

关键词： neural regeneration traditional Chinese medicine baicalin colistin sulfate PC12 cells apoptosis caspase-3 lactate dehydrogenase grants-supported paper neuroregeneration

LOV to BLUF： Flavoprotein Contributions to the Optogenetic Toolkit

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2012年第3期5卷 533-544页

作者： John M. Christie Jayde Gawthorne Gillian Young Niall J. Fraser^c and Andrew J. Roe Institute of Molecular Cell and Systems Biology College of Medical Veterinary and Life Sciences University of Glasgow UK Institute of Infection immunity and Inflammation College of Medical Veterinary and Life Sciences University of Glasgow UK Institute of Cardiovascular and medical Sciences College of Medical Veterinary and Life Sciences University of Glasgow UK

Optogenetics is an emerging field that combines optical and genetic approaches to non-invasively interfere with cellular events with exquisite spatiotemporal control. Although it arose originally from neuroscience, optogenetics is widely applicable to the study of many different biological systems and the range of applications arising from this tech- nology continues to increase. Moreover, the repertoire of light-sensitive proteins used for devising new optogenetic tools is rapidly expanding. Light, Oxygen, or Voltage sensing （LOV） and Blue-Light-Utilizing flavin adenine dinucleotide （FAD）（BLUF） domains represent new contributors to the optogenetic toolkit. These small （100-140-amino acids） flavoprotein modules are derived from plant and bacterial photoreceptors that respond to UV-A/blue light. In recent years, considerable progress has been made in uncovering the photoactivation mechanisms of both LOV and BLUF domains. This knowledge has been applied in the design of synthetic photoswitches and fluorescent reporters with applications in cell biology and biotechnology. In this review, we summarize the photochemical properties of LOV and BLUF photosensors and highlight some of the recent advances in how these flavoproteins are being employed to artificially regulate and image a variety of biological processes.

关键词： Blue-Light.Utilizing FAD chromophore flavin fluorescence light, oxygen or voltage optogenetics photo- receptor protein engineering