A Semi-quantitative Serological Method to Assess the Potency of Inactivated Rabies Vaccine for Veterinary Use

作     者：Ye Liu Shoufeng Zhang Fei Zhang Rongliang Hu 

作者机构：Laboratory of EpidemiologyMilitary Veterinary Research InstituteAcademy of Military Medical SciencesChangchun 130122China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2012年第27卷第4期

页      面：259-264页

核心收录：

学科分类：090603[农学-临床兽医学] 0710[理学-生物学] 07[理学] 09[农学] 0906[农学-兽医学] 

基　　金：the China National"863"Program(Approval No.2011AA10A212) Special Fund for Agro-Scientific Research in the Public Interest(ApprovalNo.201203056) 

主　　题：Rabies Inactivated vaccine Potency assay Semi-quantitative method 

摘      要：Potency is one of the most important indexes of inactivated vaccines.A number of methods have been established to assay the potency,of which the NIH test and single-dose mouse protection test are the prescribed methods.Here,we report a method to semi-quantitatively assay the potency of an inactivated rabies vaccine,which uses fewer animals and takes less time to *** on the quality requirements of a vaccine(*** potency),a rabies reference vaccine is,for example,diluted to the minimum potency,and 50 μL of the dilution is taken to inoculate 10 *** same amount of the test rabies vaccine is inoculated into another 10 *** two weeks,all mice are bled and serum samples are assayed for viral neutralizing antibody by the fluorescent antibody virus neutralization(FAVN) *** comparing the median and interquartile range of antibody titers of the reference vaccine with those of the test vaccine,the test vaccine potency can be semi-quantitatively judged as to whether it is in accord with the required *** reliability of this method was also confirmed in *** procedure can be recommended for batch potency testing during inactivated rabies vaccine production.