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Deep self-learning enables fast,high-fidelity isotropic resolution restoration for volumetric fluorescence microscopy

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Light(science & Applications) 2023年第9期12卷 1943-1960页

作者： Kefu Ning Bolin Lu Xiaojun Wang Xiaoyu Zhang Shuo Nie Tao Jiang Anan Li Guoqing Fan Xiaofeng Wang Qingming Luo Hui Gong Jing Yuan Britton Chance Center for biomedical Photonics Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhanChina MoE Key Laboratory for biomedical Photonics School of Engineering SciencesHuazhong University of Science and TechnologyWuhanChina HUST-Suzhou institute for Brainsmatics SuzhouChina School of biomedical Engineering Hainan UniversityHaikouChina

One intrinsic yet critical issue that troubles the field of fluorescence microscopy ever since its introduction is the unmatched resolution in the lateral and axial directions(i.e.,resolution anisotropy),which severely deteriorates the quality,reconstruction,and analysis of 3D volume *** leveraging the natural anisotropy,we present a deep self-learning method termed Self-Net that significantly improves the resolution of axial images by using the lateral images from the same raw dataset as rational *** incorporating unsupervised learning for realistic anisotropic degradation and supervised learning for high-fidelity isotropic recovery,our method can effectively suppress the hallucination with substantially enhanced image quality compared to previously reported *** the experiments,we show that Self-Net can reconstruct high-fidelity isotropic 3D images from organelle to tissue levels via raw images from various microscopy platforms,e.g.,wide-field,laser-scanning,or super-resolution *** the first time,Self-Net enables isotropic whole-brain imaging at a voxel resolution of 0.2×0.2×0.2μm^(3),which addresses the last-mile problem of data quality in single-neuron morphology visualization and reconstruction with minimal effort and ***,Self-Net is a promising approach to overcoming the inherent resolution anisotropy for all classes of 3D fluorescence microscopy.

关键词： Deep enable resolution

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Laser light scattering technique for non-invasive in situ simultaneous measurements on elastic constants and viscosity coefficients of nematic liquid crystals

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science China(Physics,Mechanics & Astronomy) 2004年第1期47卷 79-85页

作者： TU Mei HUANG Yaoxiong institute of biomedical Engineering Jinan University Guangzhou 510632 China Institute of Biomedical Engineering Jinan University Guangzhou 510632 China

The laser light scattering technique for non-invasivein situ simultaneous measurements on elastic constants and viscosity coefficients of nematic liquid crystals is introduced. By measuring the autocorrelation function of the scattered light from nematic liquid crystals at different scattering angles, the splay and twist elastic constantsK 11 andK 22 are obtained from the amplitudes of the autocorrelation function, and the viscosity coefficients ofη Splay andη Twist are determined using the viscoelastic ratiosK 11/η Splay andK 22 η Twist from the telaxation parameters of the two modes.

关键词： dynamic light scattering, nematic liquid crystal, elastic constants, viscosity coefficients.

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CHITOSAN AND MULTI-WALLED CARBON NANOTUBE COMPOSITE RODS

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Chinese Journal of Polymer science 2010年第5期28卷 801-806页

作者：胡巧玲 Key Laboratory of Macromolecule Synthesis and Functionalization Ministry of EducationZhejiang University institute of biomedical Macromolecules Department of Polymer Science and EngineeringZhejiang University

Multi-walled carbon nanotubes （MWNTs） and chitosan （CS） composite rods with layer-by-layer structure were prepared via in situ precipitation method. On the one hand, some MWNTs fragments with open tips played the role of nuclear agent to improve the crystallinity of CS. On the other hand, MWNTs embedded in CS matrix to absorb energy when the composite rods were destroying. Nanotubes pulled out from CS matrix, and lots of holes remained, so MWNTs could endure external stress effectively. The bending strength and bending modulus of CS/MWNTs rods （100/0.5, W/W） arrived at 130.7 MPa and 4.4 GPa respectively, increased by 34.3% and 7.3% compared with those of pure CS rods. Consequently, CS/MWNTs composite rods with excellent mechanical properties could be a novel device used for bone fracture internal fixation.

关键词： Chitosan Multi-walled carbon nanotube Nanocomposites Biomaterials.

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Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy(PAR-SIM)

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Light(science & Applications) 2024年第6期13卷 1140-1158页

作者： Xinzhu Xu Wenyi Wang Liang Qiao Yunzhe Fu Xichuan Ge Kun Zhao Karl Zhanghao Meiling Guan Xin Chen Meiqi Li Dayong Jin Peng Xi Department of biomedical Engineering College of Future TechnologyPeking UniversityBeijing100871China Wallace H.Coulter Dept.of biomedical Engineering Georgia Institute of Technology and Emory UniversityAtlanta30332GAUSA National biomedical Imaging Center Peking UniversityBeijing100871China Department of biomedical Engineering College of EngineeringSouthern University of Science and TechnologyShenzhen518055GuangdongChina Airy Technologies Co. Ltd.Beijing100086China Eastern institute for Advanced Study Eastern Institute of TechnologyNingboZhejiang315200China School of Life science Peking UniversityBeijing100871China institute for biomedical Materials and Devices(IBMD) Faculty of ScienceUniversity of Technology SydneySydneyNSW2007Australia

Structured illumination microscopy(SIM)has emerged as a promising super-resolution fluorescence imaging technique,offering diverse configurations and computational strategies to mitigate phototoxicity during real-time imaging of biological *** efforts to enhance system frame rates have concentrated on processing algorithms,like rolling reconstruction or reduced frame reconstruction,or on investments in costly sCMOS cameras with accelerated row readout *** this article,we introduce an approach to elevate SIM frame rates and region of interest(ROI)coverage at the hardware level,without necessitating an upsurge in camera expenses or intricate ***,parallel acquisition-readout SIM(PAR-SIM)achieves the highest imaging speed for fluorescence imaging at currently available detector *** using the full frame-width of the detector through synchronizing the pattern generation and image exposure-readout process,we have achieved a fundamentally stupendous information spatial-temporal flux of 132.9 MPixels·s^(−1),9.6-fold that of the latest techniques,with the lowest SNR of−2.11 dB and 100 nm ***-SIM demonstrates its proficiency in successfully reconstructing diverse cellular organelles in dual excitations,even under conditions of low signal due to ultra-short exposure ***,mitochondrial dynamic tubulation and ongoing membrane fusion processes have been captured in live COS-7 cell,recorded with PAR-SIM at an impressive 408 *** posit that this novel parallel exposure-readout mode not only augments SIM pattern modulation for superior frame rates but also holds the potential to benefit other complex imaging systems with a strategic controlling approach.

关键词： hardware illumination resolution

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A mini-review and perspective on ferroptosis-inducing strategies in cancer therapy

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Chinese Chemical Letters 2019年第4期30卷 847-852页

作者： Shuaifei Wang Hongwei Liao Fangyuan Li Daishun Ling institute of Pharmaceutics College of Pharmaceutical Sciences Zhejiang University Hangzhou institute of Innovative Medicine College of Pharmaceutical Sciences Zhejiang University Key Laboratory of biomedical Engineering of the Ministry of Education College of Biomedical Engineering & Instrument Science Zhejiang University

Ferroptosis, as a new type of cell death caused by lipid peroxidation, has attracted much attention since it was first identified in 2012. A lot of progress has been made in unraveling its mechanisms and therapeutic potential as a target for cancer therapy. Hitherto, there are mainly two strategies widely adopted for designing ferroptosis-inducing agents, which include increasing the intracellular reactive oxygen species(ROS) level by Fenton reaction, and inactivating the glutathione peroxidase 4(GPX4). In this mini-review,we summarize the recent advances in ferroptosis-based anticancer treatments with a highlight on nanomaterials, and discuss the current challenges faced by those agents from the perspective of in vivo applications. Moreover, by generalizing ferroptosis induced by excess iron ions to cell death caused by the polyvalent metal-mediated oxidative burden, we introduce a new paradigm of cancer treatment by exploiting various polyvalent metals to disrupt the vulnerable redox balance in cancer cells, which may greatly diversify our arsenal to combat cancer.

关键词： Ferroptosis Fenton reaction Reactive oxygen species Lipid peroxidation Glutathione peroxidase 4

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Highly Efficient and Economical Baculovirus Expression System for Preparing Human Papillomavirus Type16 Virus-like Particle

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 548-552页

作者： Jin ZHENG, Jun MA, Xiao-Feng YANG, Hong-Li LIU, Hong-Wei CHENG, Lu-Sheng SI, and Yi-Li WANGThe Key Laboratory of biomedical Information Engineering of Ministry of Education, institute of Cancer Research,School of Life science and Technology, Xi’an Jiaotong University, Xi’an 710061, China The Key Laboratory of biomedical Information Engineering of Ministry of Education Institute of Cancer Research School of Life Science and Technology Xi’an Jiaotong University

To improve the existing human papillomavirus type16 (HPV16) virus-like particle (VLP) preparation, a highly efficient, economical and timesaving system was established. Sf-9 cells were infected with recombinant baculovirus containing the target gene encoding HPV16L1 protein with 6×His tag, and harvested 72 h postinfection (p.i.) at 27 °C. The ProBondTM purification system was used for protein purification. The molecular weight of expressed HPV16L1 protein was 58 kD as revealed by SDS-PAGE, and confirmed by Western blot. The purity of denatured and native HPVL1 proteins that were prepared were 91.9% and 71.5%, respectively, which corresponded to a yield of 2.26 mg denatured protein and 1.84 mg native protein per 2×107 cells. The proteins were further analyzed by mouse erythrocyte hemagglutination assay and hemagglutination inhibition assay, and there effects on VLP formation were also visualized by transmission electron microscopy. Results showed that the native protein purified was biologically active as natural HPVL1 protein, inducing the murine erythrocyte agglutination and VLP formation. In addition, the purified recombinant HPV16L1 native protein with 6×His tag could self-assemble into virions in vitro. Hopefully, the present expression and purification system is promising to be convenient, timesaving and economical for preparation of HPV16 VLP vaccine.

关键词： human papillomavirus HPV16L1 protein purification virus-like particle (VLP)

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Microscopic inspection and tracking of single upconversion nanoparticles in living cells

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Light(science & Applications) 2018年第1期7卷 1034-1039页

作者： Fan Wang Shihui Wen Hao He Baoming Wang Zhiguang Zhou Olga Shimoni Dayong Jin institute for biomedical Materials and Devices(IBMD) Faculty of ScienceUniversity of Technology SydneySydneyNew South Wales 2007Australia

Nanoparticles have become new tools for cell biology imaging1,sub-cellular sensing2,super-resolution imaging3,4 and drug ***-term 3D tracking of nanoparticles and their intracellular motions have advanced the understanding of endocytosis and exocytosis as well as of active transport processes6–*** sophisticated operation of correlative optical-electron microscopy9,10 and scientific-grade cameras is often used to study intercellular ***,most of these studies are still limited by the insufficient sensitivity for separating a single nanoparticle from a cluster of nanoparticles or their aggregates8,11,*** we report that our eyes can track a single fluorescent nanoparticle that emits over 4000 photons per 100 milliseconds under a simple microscope *** tracking a single nanoparticle with high temporal,spectral and spatial resolution,we show the measurement of the local viscosity of the intracellular ***,beyond the colour domain and 3D position,we introduce excitation power density as the fifth dimension for our eyes to simultaneously discriminate multiple sets of single nanoparticles.

关键词： nanoparticles separating excitation

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Gene Therapy Activates Retinal Pigment Epithelium Cell Proliferation for Age-related Macular Degeneration in a Mouse Model

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Current Medical science 2023年第2期43卷 384-392页

作者： Yun YUAN Wen KONG Xiao-mei LIU Guo-hua SHI School of biomedical Engineering(Suzhou) Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaSuzhou215000China Suzhou institute of biomedical Engineering and Technology Chinese Academy of ScienceSuzhou215000China

Objective Age-related macular degeneration(AMD)is a degenerative retinal *** degeneration or death of retinal pigment epithelium(RPE)cells is implicated in the pathogenesis of *** study aimed to activate the proliferation of RPE cells in vivo by using an adeno-associated virus(AAV)vector encodingβ-catenin to treat AMD in a mouse *** Mice were intravitreally injected with AAV2/8-Y733F-VMD2-β-catenin for 2 or 4 weeks,andβ-catenin expression was measured using immunofluorescence staining,real-time quantitative reverse transcription polymerase chain reaction(PCR),and Western *** function ofβ-catenin was determined using retinal flat mounts and laser-induced damage ***,the safety of AAV2/8-Y733F-VMD2-β-catenin was evaluated by multiple intravitreal *** AAV2/8-Y733F-VMD2-β-catenin induced the expression ofβ-catenin in RPE *** activated the proliferation of RPE cells and increased cyclin D1 *** was beneficial to the recovery of laser-induced damage by activating the proliferation of RPE ***,it could induce apoptosis of RPE cells by increasing the expression of Trp53,Bax and caspase3 while decreasing the expression of *** AAV2/8-Y733F-VMD2-β-catenin increasedβ-catenin expression in RPE cells,activated RPE cell proliferation,and helped mice heal from laser-induced eye ***,it could induce the apoptosis of RPE ***,it may be a safe approach for AMD treatment.

关键词： gene therapy adeno-associated virus age-related macular degeneration retinal pigment epithelium cells β-catenin

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Synthetic biodegradable functional polymers for tissue engineering:a brief review

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science China Chemistry 2014年第4期57卷 490-500页

作者： GUO BaoLin MA Peter X Center for biomedical Engineering and Regenerative Medicine Frontier Institute of Science and Technology Xi'an Jiaotong University Department of biomedical Engineering University of Michigan Department of Biologic and Materials sciences University of Michigan Macromolecular science and Engineering Center University of Michigan Department of Materials science and Engineering University of Michigan

Scaffolds play a crucial role in tissue engineering. Biodegradable polymers with great processing flexibility are the predominant scaffolding materials. Synthetic biodegradable polymers with well-defined structure and without immunological concerns associated with naturally derived polymers are widely used in tissue engineering. The synthetic biodegradable polymers that are widely used in tissue engineering, including polyesters, polyanhydrides, polyphosphazenes, polyurethane, and poly(glycerol sebacate) are summarized in this article. New developments in conducting polymers, photoresponsive polymers, amino-acid-based polymers, enzymatically degradable polymers, and peptide-activated polymers are also discussed. In addition to chemical functionalization, the scaffold designs that mimic the nano and micro features of the extracellular matrix(ECM) are presented as well, and composite and nanocomposite scaffolds are also reviewed.

关键词： synthetic biodegradable polymers functional polymers scaffolds tissue engineering

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Autism spectrum disorder model mice: Focus on copy number variation and epigenetics

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science China(Life sciences) 2015年第10期58卷 976-984页

作者： Nobuhiro NAKAI Susumu OTSUKA Jihwan MYUNG Toru TAKUMI Graduate School of biomedical sciences Hiroshima University Graduate School of Biostudies Kyoto University RIKEN Brain science institute Core Research for Evolutional science and Technology Japan Science and Technology Agency

Autism spectrum disorder(ASD) is gathering concerns in socially developed countries. ASD is a neuropsychiatric disorder of genetic origin with high prevalence of 1%–2%. The patients with ASD characteristically show impaired social skills. Today, many genetic studies identify numerous susceptible genes and genetic loci associated with ASD. Although some genetic factors can lead to abnormal brain function linked to ASD phenotypes, the pathogenic mechanism of ASD is still unclear. Here, we discuss a new mouse model for ASD as an advanced tool to understand the mechanism of ASD.

关键词： copy number variations DNA methylation histone modification ASD model mouse

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