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Acquired sensorineural hearing loss,oxidative stress,and microRNAs

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Neural Regeneration Research 2025年第9期20卷 2513-2519页

作者： Desmond A.Nunez Ru C.Guo Division of Otolaryngology–Head&Neck Surgery Department of SurgeryFaculty of MedicineThe University of British ColumbiaVancouverBCCanada Division of Otolaryngology–Head&Neck Surgery Gordon&Leslie Diamond Health Care CentreVancouver General HospitalVancouverBCCanada Faculty of Medicine–The university of British columbia VancouverBCCanada

Hearing loss is the third leading cause of human ***-related hearing loss,one type of acquired sensorineural hearing loss,is largely responsible for this escalating global health ***-induced,ototoxic,and idiopathic sudden sensorineural are other less common types of acquired hearing *** etiology of these conditions is complex and multi-fa ctorial involving an interplay of genetic and environmental *** stress has recently been proposed as a likely linking cause in most types of acquired sensorineural hearing *** non-coding RNA sequences known as microRNAs(miRNAs)have increasingly been shown to play a role in cellular hypoxia and oxidative stress responses including promoting an apoptotic *** hair cell death is a central histopathological finding in sensorineural hearing *** these cells do not regenerate in humans,it underlies the irreversibility of human age-related hearing *** EMBASE,Ovid MEDLINE,Web of Science Core Collection,and *** databases over the period August 1,2018 to July 31,2023 were searched with"hearing loss,""hypoxamiRs,""hypoxia,""microRNAs,""ischemia,"and"oxidative stress"text words for English language primary study publications or registered clinical *** red clinical trials known to the senior author we re also assessed.A total of 222studies were thus *** excluding duplicates,editorials,retra ctions,secondary research studies,and non-English language articles,39 primary studies and clinical trials underwent full-text *** resulted in 11 animal,in vitro,and/or human subject journal articles and 8 registered clinical trial database entries which form the basis of this narrative *** miR-34a and miR-29b levels increase with age in *** miRNAs were demonstrated in human neuroblastoma and murine cochlear cell lines to target Sirtuin 1/peroxisome proliferato r-activated receptor gamma coactivator-1-alpha(SIRT1/P GC-1α),SIRT

关键词： hearing loss hypoxia microRNAs oxidative stress sensorineural

Interrelationship of soil moisture and temperature to sylvatic plague cycle among prairie dogs in the Western United States

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Integrative Zoology 2021年第6期16卷 852-867页

作者： Cassandra D.PAULING Deborah L.FINKE Deborah M.ANDERSON Department of Veterinary Pathobiology University of MissouriColumbiaMissouriUSA Division of Plant Sciences University of MissouriColumbiaMissouriUSA

Plague,caused by Yersinia pestis,is a flea-borne disease that is endemic in areas throughout the world due to its successful maintenance in a sylvatic cycle,mainly in areas with temperate *** rodents are thought to play a key role in the enzootic maintenance as well as epizootic outbreaks of *** the United States,prairie dogs(Cynomys),rodents(Muridae),and ground squirrels(Spermophilus)are susceptible to infection and are parasitized by fleas that transmit *** particular,prairie dogs can experience outbreaks that rapidly spread,which can lead to extirpation of colonies.A number of ecological parameters,including climate,are associated with these *** this study,we asked whether soil parameters,primarily moisture and temperature,are associated with outbreaks of plague in black-tailed prairie dogs and Gunnison’s prairie dogs in the Western United States,and at what depth these associations were *** collected publicly available county-level information on the occurrence of population declines or colony extirpation,while historical soil data was collected from SCAN and USCRN stations in counties and states where prairie dogs have been *** analysis suggests that soil moisture at lower depths correlates with colony die-offs,in addition to temperature near the surface,with key differences within the landscape ecology that impact the occurrence of ***,the model suggests that the burrow environment may play a significant role in the epizootic spread of disease amongst black-tailed and Gunnison’s prairie dogs.

关键词： plague prairie dogs soil moisture soil temperature sylvatic cycle Yersinia pestis

A microfluidic biosensor for the diagnosis ofchronic wasting disease

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Microsystems & Nanoengineering 2023年第4期9卷 263-279页

作者： Sura A.Muhsin Amjed Abdullah Estela kobashigawa Muthana Al-Amidie Sherri Russell Michael Z.Zhang Shuping Zhang Mahmoud Almasri university of missouri–columbia Electrical Engineering and Computer ScienceColumbiaMOUSA university of missouri–columbia College of Veterinary MedicineVeterinary Medical Diagnostic LaboratoryColumbiaMOUSA missouri Department of Conservation ColumbiaMOUSA

Cervids are affected by a neurologic disease that is always fatal to individuals and has population effects. This disease iscalled chronic wasting disease (CWD) and is caused by a misfolded prion protein. The disease is transmitted viacontact with contaminated body fluids and tissue or exposure to the environment, such as drinking water or *** CWD diagnosis depends on ELISA screening of cervid lymph nodes and subsequent immunohistochemistry(IHC) confirmation of ELISA-positive results. The disease has proven to be difficult to control in part because ofsensitivity and specificity issues with the current test regimen. We have investigated an accurate, rapid, and low-costmicrofluidic microelectromechanical system (MEMS) biosensing device for the detection of CWD pathologic prions inretropharyngeal lymph nodes (RLNs), which is the current standard type of CWD diagnostic sample. The deviceconsists of three novel regions for concentrating, trapping, and detecting the prion. The detection region includes anarray of electrodes coated with a monoclonal antibody against pathologic prions. The experimental conditions wereoptimized using an engineered prion control antigen. Testing could be completed in less than 1 hour with highsensitivity and selectivity. The biosensor detected the engineered prion antigen at a 1:24 dilution, while ELISA detectedthe same antigen at a 1:8 dilution. The relative limit of detection (rLOD) of the biosensor was a 1:1000 dilution of aknown strong positive RLN sample, whereas ELISA showed a rLOD of 1:100 dilution. Thus, the biosensor was 10 timesmore sensitive than ELISA, which is the currently approved CWD diagnostic test. The biosensor’s specificity andselectivity were confirmed using known negative RPLN samples, a negative control antibody (monoclonal antibodyagainst bovine coronavirus BCV), and two negative control antigens (bluetongue virus and Epizootic hemorrhagicdisease virus). The biosensor’s ability to detect pathogenic prions was

关键词： fir diagnosis specificity

Laser-induced graphene for bioelectronics and soft actuators

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Nano Research 2021年第9期14卷 3033-3050页

作者： Yadong Xu Qihui Fei Margaret Page Ganggang Zhao Yun Ling Dick Chen Zheng Yan Department of Biomedical Biological&Chemical EngineeringUniversity of MissouriColumbiaMissouri 65211USA Department of Mechanical&Aerospace Engineering University of MissouriColumbiaMissouri 65211USA Rock Bridge High School ColumbiaMissouri 65203USA

Laser-assisted process can enable facile,mask-free,large-area,inexpensive,customizable,and miniaturized patterning of laser-induced porous graphene(LIG)on versatile carbonaceous substrates(e.g.,polymers,wood,food,textiles)in a programmed manner at ambient *** with high tailorability of its porosity,morphology,composition,and electrical conductivity,LIG can find wide applications in emerging bioelectronics(e.g.,biophysical and biochemical sensing)and soft robots(e.g.,soft actuators).In this review paper,we first introduce the methods to make LIG on various carbonaceous substrates and then discuss its electrical,mechanical,and antibacterial properties and biocompatibility that are critical for applications in bioelectronics and soft ***,we overview the recent studies of LIG-based biophysical(e.g.,strain,pressure,temperature,hydration,humidity,electrophysiological)sensors and biochemical(e.g.,gases,electrolytes,metabolites,pathogens,nucleic acids,immunology)*** applications of LIG in flexible energy generators and photodetectors are also *** addition,LIG-enabled soft actuators that can respond to chemicals,electricity,and light stimulus are ***,we briefly discuss the future challenges and opportunities of LIG fabrications and applications.

关键词： laser-induced graphene bioelectronics biophysical sensors biochemical sensors soft actuators

Most Likely Optimal Subsampled Markov Chain Monte Carlo

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Journal of Systems Science & Complexity 2021年第3期34卷 1121-1134页

作者： HU Guanyu WANG Haiying university of missouri-columbia USA university of Connecticut USA

Markov Chain Monte Carlo(MCMC) requires to evaluate the full data likelihood at different parameter values iteratively and is often computationally infeasible for large data sets. This paper proposes to approximate the log-likelihood with subsamples taken according to nonuniform subsampling probabilities, and derives the most likely optimal(MLO) subsampling probabilities for better approximation. Compared with existing subsampled MCMC algorithm with equal subsampling probabilities,the MLO subsampled MCMC has a higher estimation efficiency with the same subsampling ratio. The authors also derive a formula using the asymptotic distribution of the subsampled log-likelihood to determine the required subsample size in each MCMC iteration for a given level of precision. This formula is used to develop an adaptive version of the MLO subsampled MCMC algorithm. Numerical experiments demonstrate that the proposed method outperforms the uniform subsampled MCMC.

关键词： Big data MCMC metropolis-hasting algorithm nonuniform subsampling

Characterization of the surface and interfacial properties of the lamina splendens

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Frontiers of Mechanical Engineering 2017年第2期12卷 234-252页

作者： Joe T. REXWINKLE Heather K. HUNT Ferris M. PFEIFFER Mechanical Engineering University of Missouri Columbia MO 65211USA Bioengineering University of Missouri Columbia MO 65211 USA Orthopaedic Surgery University of Missouri Columbia MO 65211USA

Joint disease affects approximately 52.5 mil- lion patients in the United States alone, costing 80.8 billion USD in direct healthcare costs. The development of treatment programs for joint disease and trauma requires accurate assessment of articular cartilage degradation. The articular cartilage is the interfacial tissue between articu- lating surfaces, such as bones, and acts as low-friction interfaces. Damage to the lamina splendens, which is the articular cartilage＇s topmost layer, is an early indicator of joint degradation caused by injury or disease. By gaining comprehensive knowledge on the lamina splendens, particularly its structure and interfacial properties, researchers could enhance the accuracy of human and animal biomechanical models, as well as develop appro- priate biomimetic materials for replacing damaged articu- lar cartilage, thereby leading to rational treatment programs for joint disease and injury. Previous studies that utilize light, electron, and force microscopy techniques have found that the lamina splendens is composed of collagen fibers oriented parallel to the cartilage surface and encased in a proteoglycan matrix. Such orientation maximizes wear resistance and proteoglycan retention while promoting the passage of nutrients and synovial fluid. Although the structure of the lamina splendens has been explored in the literature, the low-friction interface of this tissue remains only partially characterized. Various functional models are currently available for the interface, such as pure boundary lubrication, thin films exuded under pressure, and sheets of trapped proteins. Recent studies suggest that each of these lubrication models has certain advantages over one another. Further research is needed to fully model the interface of this tissue. In this review, we summarize the methods for characterizing the lamina splendens and the results of each method. This paper aims to serve as a resource for existing studies to date and a roadmap of t

关键词： cartilage lamina splendens characterization,biomechanics orthopaedic review

Predicting carbon nanotube forest attributes and mechanical properties using simulated images and deep learning

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npj Computational Materials 2021年第1期7卷 1214-1224页

作者： Taher Hajilounezhad Rina Bao Kannappan Palaniappan Filiz Bunyak Prasad Calyam Matthew R.Maschmann Mechanical&Aerospace Engineering University of MissouriColumbiaMOUSA Electrical Engineering and Computer Science University of MissouriColumbiaMOUSA

Understanding and controlling the self-assembly of vertically oriented carbon nanotube(CNT)forests is essential for realizing their potential in myriad *** governing process–structure–property mechanisms are poorly understood,and the processing parameter space is far too vast to exhaustively explore *** overcome these limitations by using a physics-based simulation as a high-throughput virtual laboratory and image-based machine learning to relate CNT forest synthesis attributes to their mechanical *** CNTNet,our image-based deep learning classifier module trained with synthetic imagery,combinations of CNT diameter,density,and population growth rate classes were labeled with an accuracy of>91%.The CNTNet regression module predicted CNT forest stiffness and buckling load properties with a lower root-mean-square error than that of a regression predictor based on CNT physical *** results demonstrate that image-based machine learning trained using only simulated imagery can distinguish subtle CNT forest morphological features to predict physical material properties with high *** paves the way to incorporate scanning electron microscope imagery for high-throughput material discovery.

关键词： carbon properties overcome

Overlapping functions of YDA and MAPKKK3/MAPKKK5 upstream of MPK3/MPK6 in plant immunity and growth/development

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Journal of Integrative Plant Biology 2022年第8期64卷 1531-1542页

作者： Yidong Liu Emma Leary Obai Saffaf RFrank Baker Shuqun Zhang Division of Biochemistry University of MissouriColumbiaMO 65211USA Division of Biological Sciences University of MissouriColumbiaMO 65211USA Advanced Light Microscopy Core University of MissouriColumbiaMO 65211USA

Arabidopsis MITOGEN-ACTIVATED PROTEIN KINASE3(MAPK3 or MPK3)and MPK6 play important signaling roles in plant immunity and growth/*** KINASE4(MKK4)and MKK5 function redundantly upstream of MPK3 and MPK6 in these ***(YDA),also known as MAPK KINASE KINASE4(MAPKKK4),is upstream of MKK4/MKK5 and forms a complete MAPK cascade(YDA–MKK4/MKK5–MPK3/MPK6)in regulating plant growth and *** plant immunity,MAPKKK3 and MAPKKK5 function redundantly upstream of the same MKK4/MKK5–MPK3/MPK6 ***,the residual activation of MPK3/MPK6 in the mapkkk3 mapkkk5 double mutant in response to flg22 pathogen-associated molecular pattern(PAMP)treatment suggests the presence of additional MAPKKK(s)in this MAPK cascade in signaling plant *** investigate whether YDA is also involved in plant immunity,we attempted to generate mapkkk3 mapkkk5 yda triple ***,it was not possible to recover one of the double mutant combinations(mapkkk5 yda)or the triple mutant(mapkkk3 mapkkk5 yda)due to a failure of *** the clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPRassociated protein 9(Cas9)approach,we generated weak,N-terminal deletion alleles of YDA,yda-del,in a mapkkk3 mapkkk5 ***-triggered MPK3/MPK6 activation was further reduced in the mapkkk3 mapkkk5 yda-del mutant,and the triple mutant was more susceptible to pathogen infection,suggesting YDA also plays an important role in plant immune *** addition,MAPKKK5 and,to a lesser extent,MAPKKK3 were found to contribute to gamete function and embryogenesis,together with *** the double homozygous mapkkk3 yda mutant showed the same growth and development defects as the yda single mutant,mapkkk5 yda double mutant and mapkkk3 mapkkk5 yda triple mutants were embryo lethal,similar to the mpk3 mpk6 double *** results demonstrate that YDA,MAPKKK3,and MAPKKK5 have overlapping functions upstream of the MKK4/MKK5–MPK3/MPK6 module in bot

关键词： embryogenesis gamete transmission MAPK cascade MPK3/MPK6 MAPKKK3/MAPKKK5 plant immunity YDA

Trilayer anisotropic structure versus randomly oriented structure in heart valve leaflet tissue engineering

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Bio-Design and Manufacturing 2023年第4期6卷 423-438页

作者： Yuriy Snyder Soumen Jana Department of Bioengineering University of MissouriColumbiaMO 65211USA

It has been hypothesized that leaflet substrates with a trilayer structure and anisotropicmechanical properties could be useful for the production of functional and long-lasting tissue-engineered *** investigate the influence of the anisotropic structural and mechanical characteristics of a substrate on cells,in this study,we electrospun trilayer anisotropic fibrous substrates and randomly oriented isotropic fibrous substrates(used as controls)from polycaprolactone ***,the random substrates had higher radial and lower circumferential tensile properties than the trilayer substrates;however,they had similar flexural *** valvular interstitial cells cultured on both substrates produced random and trilayer cell-cultured constructs,*** trilayer cell-cultured constructs had more anisotropic mechanical properties,17%higher cellular proliferation,14%more extracellular matrix(i.e.,collagen and glycosaminoglycan)production,and superior gene and protein expression,suggesting that more cells were in a growth state in the trilayer constructs than in the random ***,the random and radial layers of the trilayer constructs had more vimentin,collagen,transforming growth factor-beta 1(TGF-ß1),transforming growth factor-beta 3(TGF-ß3)gene expression than in the circumferential layer of the *** study verifies that the differences in structural,tensile,and anisotropic properties of the trilayer and random substrates influence the characteristics of the cells and ECM in the constructs.

关键词： Heart valve leaflet Trilayer Tissue engineering Fiber Electrospinning