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Candida albicans isolated from urine:Phenotypic and molecular identification,virulence factors and antifungal susceptibility

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Asian Pacific Journal of Tropical Biomedicine 2017年第7期7卷 624-628页

作者： Laura Wiebusch Adriana Araújo de Almeida-Apolonio Luana Mireli Carbonera Rodrigues Bruna de Paula Bicudo danielly Beraldo dos Santos Silva danielle Ferreira Lonchiati Renata Pires de Araujo Alexéia Barufatti Grisolia Kelly Mari Pires de Oliveira Faculdade de Ciencias Biologicas e Ambientais Universidade Federal da Grande DouradosDouradosMSBrazil Faculdade de Medicina Universidade Federal de Mato Grosso do SulCampo GrandeMSBrazil Faculdade de Ciencias da Saude Universidade Federal da Grande DouradosDouradosMSBrazil Faculdade de Ciencias Agrarias e Veterinarias Universidade Estadual Paulista“Julio de Mesquita Filho”JaboticabalSPBrazil

Objective: To isolate Candida albicans(C. albicans) from the urine of hospitalized patients and assess the virulence factors and antifungal susceptibility profiles of the ***: Yeasts were identified using the chromogenic medium CHROMagar?, the VITEK~?2 system, hypertonic Sabouraud broth, tobacco agar, polymerase chain reaction,and DNA sequencing. The evaluated virulence factors were proteinase production,phospholipase production, and biofilm production on polystyrene. The broth microdilution technique was used to determine the minimum inhibitory ***: All yeasts isolated from urine were identified as C. albicans using both classical and molecular methods. Although 91.3% of the isolates showed no phospholipase activity, 56.5% showed strong proteinase activity and 91.7% produced biofilm. All microorganisms were sensitive to fluconazole, voriconazole and amphotericin B, but 56.5% of the yeasts showed resistance to ***: C. albicans isolates from urine have a high capacity for virulence and can be associated with infectious processes. Furthermore, the high percentage of isolates resistant to itraconazole is important because this antifungal agent is commonly used to treat fungal infections in the hospital environment.

关键词： Candidiasis Urinary tract Infection Virulence Antifungal susceptibility

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Aerodynamic Effects Compensation on Multi-Rotor UAVs Based on a Neural Network Control Allocation Approach

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IEEE/CAA Journal of Automatica Sinica 2022年第2期9卷 295-312页

作者： Sarah P.Madruga Augusto H.B.M.Tavares Saulo O.D.Luiz Tiago P.do Nascimento Antonio Marcus N.Lima Graduate Program of Electrical Engineering Universidade Federal de Campina GrandeCampina Grande-PBBrazil Department of Electrical Engineering Universidade Federal de Campina GrandeCampina Grande-PBBrazil IEEE Department of Computer Systems Universidade Federal da ParaibaJoao Pessoa-PBBrazil

This paper shows that the aerodynamic effects can be compensated in a quadrotor system by means of a control allocation approach using neural ***,the system performance can be improved by replacing the classic allocation matrix,without using the aerodynamic inflow equations *** network training is performed offline,which requires low computational *** target system is a Parrot MAMBO drone whose flight control is composed of PD-PID controllers followed by the proposed neural network control allocation *** a quadrotor is particularly susceptible to the aerodynamics effects of interest to this work,because of its small *** compared the mechanical torques commanded by the flight controller,i.e.,the control input,to those actually generated by the actuators and established at the *** was observed that the proposed neural network was able to closely match them,while the classic allocation matrix could not achieve *** allocation error was also determined in both ***,the closed-loop performance also improved with the use of the proposed neural network control allocation,as well as the quality of the thrust and torque signals,in which we perceived a much less noisy behavior.

关键词： Aerodynamics effects control allocation minidrone multi-rotor UAV neural networks

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Common beans (Phaseolus vulgaris L.) in the rations for cattle in feedlot

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Agricultural Sciences 2013年第12期4卷 774-780页

作者： Rafael Henrique de Tonissi e Buschinelli de Goes Karl Whays Klein Luis Henrique Martinhago Euclides Reuter de Oliveira Kelly Cristina da Silva Brabes Maria Gizelma de Menezes Gressler Mayara Mitiko Yoshiraha Raquel Tenório de Oliveira Etelvitor Martins Leite dos Santos universidade federal da grande dourados (UFGD) Faculdade de Ciências Agrárias Dourados Brasil universidade federal da grande dourados (UFGD) Faculdade de Engenharia Dourados Brasil

To evaluate the inclusion of common beans in the diet of cattle in feedlot on characteristics of productive performance, nutrient digestibility and ruminal fermentation parameters, 15 bulls crossed were used in a completely randomized design. The animals were confined for 64 days, and 21 days of adaptation. We evaluated the inclusion common beans at levels of 0%, 13% and 26%. All diets were isocaloric and isonitrogenous with a 45:55 forage: concentrate ratio. The addition of beans in the diet did not affect animal performance, dry matter intake and feed efficiency. Inclusion bean changed the nutrient digestibility of DM, CP, EE, TC and OM, where the best digestibility was observed with the inclusion 13% and the lowest concentration of ruminal NH3-N. The general behavior of ruminal pH in relation to levels of inclusion of beans was 6.2. The inclusion of up to 13% of beans in the diet of feedlot cattle did not alter the apparent digestibility, ruminal and animal performance.

关键词： Average daily Gain Digestibility Ruminal Parameters Urea

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Storage of Campomanesia adamantium(Cambess.)*** Seeds:Influence of Water Content and Environmental Temperature

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American Journal of Plant Sciences 2014年第17期5卷 2555-2565页

作者： daiane Mugnol Dresch Silvana de Paula Quintao Scalon Tathiana Elisa Masetto Rosilda Mara Mussury Pos-Doutoranda em Agronomia(PNPD/CAPES) Universidade Federal da Grande Dourados(UFGD)DouradosBrasil Faculdade de Ciencias Agrarias Universidade Federal da Grande Dourados(UFGD)DouradosBrasil Faculdade de Ciencias Biologicas e Ambientais Universidade Federal da Grande Dourados(UFGD)DouradosBrasil

The present work evaluated the conservation of Campomanesia adamantium seeds under different conditions. The fruits used in the study were collected from matrices located in the city of Ponta Porã-MS, Brazil. After processing, the seeds were slowly dried to different water content levels and subsequently exposed to various environmental conditions: 25°C ± 2°C, 35% relative humidity (laboratory);16°C ± 1°C, 40% relative humidity (cold and dry);8°C ± 1°C, 35% relative humidity (refrigeration);and -18°C ± 1°C, 42% relative humidity (freezing). Each treatment was conducted for 0 (recently processed seeds, with superficial drying of 40 minutes), 30, 60, 90, 120, 150, and 180 days. Seed potential was physiologically evaluated based on radicle protrusion, percentage of normal seedlings, seedling length (primary root, shoot and total), and dry mass of the seedlings. The experimental design was a completely randomized factorial scheme with split-split plots (4 temperatures/environments × 5 water content × 7 storage periods). C. adamantium seeds tolerated a reduction in the water content to 15.3% but did not tolerate the storage period, confirming the recalcitrant behavior of the seeds.

关键词： Myrtaceae Brazilian Savanna Drying Conservation

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Fermion bound states in geometrically deformed backgrounds

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Chinese Physics C 2019年第1期43卷 1-8页

作者： D.Bazeia A.Mohammadi D.C.Moreira Departamento de Física Universidade Federal da Paraíba Departamento de Física Universidade Federal de Pernambuco Unidade Acadêmica de Física Universidade Federal de Campina Grande

This work deals with the behavior of fermions in the background of kinklike structures in the twodimensional spacetime. The kinklike structures appear from bosonic scalar field models that engender distinct profiles and interact with the fermion fields via the standard Yukawa coupling. We first consider two models that engender parity symmetry, one leading to the exclusion of fermion bound states, and the other to the inclusion of bound states, when the parameter that controls the bosonic structure varies from zero to unity. We then investigate a third model where the kinklike solution explicitly breaks parity symmetry, leading to fermion bound states that are spatially asymmetric.

关键词： topological defect fermion interaction bound state

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Quality of Jatropha curcasL. Seeds Harvested at Different Stages of Fruit Maturation

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American Journal of Plant Sciences 2014年第18期5卷 2716-2725页

作者： Cristiane de Oliveira Veronesi Cristiano Marcio Alves de Souza Ademar Pereira Serra Leidy Zulys Leyva Rafull Cesar José da Silva Vinicius de Vito Ros Vanessa do Amaral Conrad Embrapa Beef Cattle Campo Grande Brazil Embrapa Western Agriculture Dourados Brazil federal University of grande dourados (UFGD—universidade federal da grande dourados) Dourados Brazil

The aim of this study was to evaluate the physiological quality of Jatropha curcas L. seeds harvested at different stages of fruit maturation and two threshing methods. To analyze the mechanical damage rate an experiment was carried out with five stages of fruit maturation (green, yellow, ripe, dried on the ground and dried on the plant) threshed mechanically and manually. To evaluate the physiological quality, three tests were made;germination, vigor and electrical conductivity, all of them were applied in each storage (0, 120 and 240 days after harvest) and five stages of fruit maturation. It was feasible to infer that the maturation stage of yellow, ripe and dried on the plant fruits showed the best results of the physiological quality of the Jatropha curcas L. seeds. The manual threshing is also recommended in order to preserve the physiological quality of Jatropha curcas L. seeds. When the fruits were harvested at the appropriate stage of maturation (yellow, ripe and dry fruit on the plant) and threshing without mechanical damage, the Jatropha curcas L. seeds are possible to store for 240 days without loss in their germination. Regardless of the threshing system and the maturation stage, the seeds of Jatropha curcas L. lost their vigor from 120 days after harvested, when these seeds were stored in the shadow and in an environment without control on the relative humidity and air temperature.

关键词： Vigor Harvester Electrical Conductivity Germination

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Evaluation of DNA Extraction Methods for Detection of Leishmaniaby Polymerase Chain Reaction

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American Journal of Molecular Biology 2020年第4期10卷 265-272页

作者： Letícia Surian Batalini Silvana de Oliveira Castro Carla Geórgia Rodrigues Guimarães Souto Herintha Coeto Neitzke-Abreu Manoel Sebastião da Costa Lima-Junior Faculty of Health Sciences Universidade Federal da Grande Dourados (UFGD) Dourados Brazil Postgraduate Program of Health Sciences Universidade Federal da Grande Dourados (UFGD) Dourados Brazil Aggeu Magalhã es Institute Oswaldo Cruz Fundation (Fiocruz) Recife Brazil

There are several methods used to obtain DNA from cells;however, the quantity, integrity, and purity of DNA vary among the methods, which may interfere with the polymerase chain reaction (PCR) results. The objective was to determine the most efficient and cost-effective method that provides the best DNA yield and PCR results. Three methods of DNA isolation were compared: 20% sodium dodecyl sulfate (SDS), guanidine isothiocyanate-phenol-chloroform (GTPC), and DNA extraction using a commercial kit (GE Healthcare GenomicPrep Blood DNA Isolation KitTM). Human peripheral blood samples were inoculated with 104 promastigotes of Leishmania infantum. DNA was quantified and PCR was performed with 13A/13B primers. The results showed that a higher DNA yield was obtained using the GTPC technique (214.51 ng/μL), followed by SDS (26.16 ng/μL) and the commercial kit (10.99 ng/μL). We concluded that while all of the techniques were effective for obtaining DNA, the GTPC method provided the best yield and the brightest bands.

关键词： Leishmania DNA Purification PCR Diagnosis

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Fern extracts potentiate fluconazole activity and inhibit morphological changes in Candida species

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Asian Pacific Journal of Tropical Biomedicine 2017年第11期7卷 1025-1030页

作者： Maria A.Freitas Antonia T.L.Santos Antonio J.T.Machado Ana Raquel P.Silva Fábia F.Campina Maria S.Costa Gioconda M.A.B.Martins Maria Flaviana B.Morais-Braga Saulo R.Tintino Irwin R.A.Menezes Jaime Ribeiro-Filho Altevir P.Medeiros Adeliana S.Oliveira Patrício B.Maracajá Henrique D.M.Coutinho universidade Regional do Cariri-URCA universidade federal Rural do Semi-A′rido-UFERSA universidade federal do Rio grande do Norte-UFRN universidade federal de Campina grande-UFCG

Objective: To investigate the antifungal activity of the fern species Lygodium venustum(L. venustum) and Pityrogramma calomelanos(P. calomelanos) against Candida albicans and Candida tropicalis ***: The microdilution method was used to evaluate the antifungal activity, as well as the modulating effects of ethanolic extracts of these plants in combination with fluconazole. The minimum inhibitory concentration(MIC), minimum fungicide concentration and morphological changes were also ***: The extract obtained from L. venustum presented a MIC > 8 192 mg/m L, while the extract obtained from and P. calomelanos presented a MIC = 8 192 mg/m L, indicating that they present weak antifungal activity. However, combination of the extracts with Fluconazole potentiated the antifungal activity of this drug. At different experimental conditions, such as concentration of the extract and type of strain, the extracts inhibited hyphae and pseudohyphae formation, indicating that these fern species can affect the morphology of the ***: The extracts obtained from the fern species L. venustum and P. calomelanos dose not present significant antifungal activity. However, P. calomelanos potentiates the activity of fluconazole and both extracts inhibits the morphological changes in Candida species, indicating that they have potential pharmacological activity as modulators of fungal biology. Therefore, novel studies are required to characterize the interference of these extracts in the virulence and pathogenicity of Candida species as well as the potential of fern species to treat fungal infections.

关键词： Dimorphism Candida species Ferns Lygodium venustum Pityrogramma calomelanos

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Numerical Study on the Effect of Submerged Depth on the Horizontal Plate Wave Energy Converter

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China Ocean Engineering 2014年第5期28卷 687-700页

作者： Flávio Medeiros SEIBT Eduardo Costa COUTO Elizaldo Domingues dos SANTOS Liércio André ISOLDI Luiz Alberto Oliveira ROCHA Paulo Roberto de Freitas TEIXEIRA Graduate Program of Ocean Engineering Universidade Federal do Rio Grande96203-900 Rio Grande RS Brazil Mechanical Engineering Department Universidade Federal do Rio Grande do Sul90050-170 Porto Alegre RS Brazil

The growing search for clean and renewable energy sources has given rise to the studies of exploring sea wave energy. This paper is concerned with the numerical evaluation of the main operational principle of a submerged plate employed for the conversion of wave energy into electrical one. The numerical model used to solve the conservation equations of mass, momentum and transport of volume fraction is based on the finite volume method （FVM）. In order to tackle with the flow of mixture of air-water and its interaction with the device, the multiphase model volume of fluid （VOF） is employed. The purpose of this study is the evaluation of a numerical model for improvement of the knowledge about the submerged plate wave energy converter, as well as the investigation of the effect of the distance from the plate to the bottom of the sea （HP） on the performance of the converter. The simulations for several distances of the plate from the seabed show that the optimal efficiency is 64%, which is obtained for HP=0.53 m （88% of the depth）. This efficiency is 17% larger than that found in the worst case （HP=0.46 m, 77% of the depth）.

关键词： numerical simulation submerged plate wave energy conversion efficiency

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Structure,stability,catalytic activity,and polarizabilities of small iridium clusters

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Chinese Physics B 2018年第6期27卷 225-230页

作者： Francisco E Jorge José R da Costa Venaancio Unidade Academica dc Ffsica Universidade Federal de Campina Grande 58429-900 Campina Grande Brazil Departamento de Ffsica Universidade Federal do Espfrito Santo 29060-900 Vitoria Brazil

At the second order Douglas–Kroll–Hess（DKH2） level, the B3 PW91 functional in conjunction with the relativistic all-electron basis set of valence triple zeta quality plus polarization functions are employed to compute bond lengths, dissociation energies, vertical ionization potentials, and the highest occupied molecular orbital-lowest unoccupied molecular orbital energy gaps of the small iridium clusters（Irn, n≤8）. These results are compared with the experimental and theoretical data available in the literature. Our results confirm the theoretical predictions made by Feng et al. about the catalytic activities of the Ir4 and Ir6 clusters. From the optimized geometries, DKH2 calculations of static electric mean dipole polarizabilities and polarizability anisotropies are also carried out. It is the first time that the polarizabilities of small iridium clusters have been studied. For n≤4, the mean dipole polarizabilities per atom present an odd–even oscillatory behavior,whereas from Ir5 to Ir8, they decrease with the cluster size increasing. The dependence of the polarizability anisotropy on the structure symmetry of the iridium cluster is verified.

关键词： B3PW91 functional all-electron basis sets DKH2 calculations iridium clusters polarizabilities

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