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The role of vascular endothelial growth factor in ossification

International Journal of Oral Science 2012年第2期4卷 64-68页

作者： Yan-Qi Yang Ying-Ying Tan Ricky Wong Alex Wenden Lin-Kun Zhang A bakr M Rabie Department of Orthodontics Faculty of Dentistry The University of Hong Kong Hong Kong China unit pakar ortodontik Klinik Pergigian Poliklinik Komuniti Mak MandinMalaysia Department0fOrfhodonflcs Tlanj}nsfomafoI.gicalH.spJcaJ.iNankaiunIversityTianjinchinaand PrivatePracticeC0rrespondence:DrYQYang DepartmentDfOrthodonticsFacultyofDentIstryTheUnlversity硝HongKong34HospnalRoadHOngK0ngSARChinaE—maiJ=yan副anq@hkuhk

Osteogenesis and angiogenesis are two closely correlated processes during bone growth, development, remodelling and repair. Vascular endothelial growth factor （VEGF） is an essential mediator during the process of angiogenesis. Based on an extensive literature search, which was carried out using the PubMed database and the keywords of osteogenesis, VEGF, endochondral ossification and intramembranous ossification, this manuscript reviews the role of VEGF in ossification, with emphasis on its effect in endochondral and intramembranous ossification. Osteogenesis and angiogenesis are closely correlated processes. VEGF acts as an essential mediator durin~ these processes. It not only functions in bone an^io^enesis but also in various aspects of bone develooment.

关键词： endochondral ossification intramembranous ossification osteogenesis vascular endothelial growth factor

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Construction and expression of a synthetic gene encoding nonstructural glycoprotein NS1 of dengue 2 virus in Pichia pastoris

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Asian Pacific Journal of Tropical Biomedicine 2017年第8期7卷 689-693页

作者： Fernita Puspasari Riski Dwimalida Putri Aisyah Raden Roro Rika Damayanti Anita Yuwita Bachti Alisjahbana Sukwan Handali Ihsanawati Dessy Natalia Biochemistry Research Division Faculty of Mathematics and Natural SciencesInstitute of Technology Bandung pakar Biomedika Indonesia Faculty of Medicine Universitas Padjadjaran

To express and characterize NS1 of Indonesian-specific DENV2 virus in Pichia pastoris (P. pastoris).MethodsA codon optimized synthetic gene derived from the DENV-2 NS1 amino acid sequences was synthesized commercially and inserted into the P. pastoris pPICZαA expression vector. The recombinant DENV-2 NS1 protein was purified by Ni-NTA affinity chromatography, and its antigenicity was *** recombinant DENV-2 NS1 protein was secreted as a protein with a molecular weight of ∼45 kDa, and the optimal expression condition was achieved by induction with 2% (v/v) methanol for 72 h. The purified recombinant DENV-2 NS1 protein was able to interact with a monoclonal antibody of NS1 in a commercial rapid *** resulting recombinant DENV-2 NS1 protein produced in P. pastoris KM71 is a potential candidate for use in the development of a dengue diagnostic kit and vaccine.

关键词： DENV 2 Dengue virus NS1 protein Diagnostic kit Pichia pastoris

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Correlation of Hamstring Tendon Size in Pre-Operative MRI Measurement with Intra-Operative Graft Size in Primary Anterior Cruciate Ligament (ACL) Reconstruction

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Journal of Biosciences and Medicines 2024年第3期12卷 150-158页

作者： Nurhanani Abu Bakar Mohamad Razip Sirat Hairiah Abas Saiful Safuan Md Sani Department of Orthopaedic Hospital Pakar Sultanah Fatimah Muar Malaysia Department of Orthopaedic Hospital Tengku Ampuan Rahimah Klang Malaysia Department of Radiology Hospital Pakar Sultanah Fatimah Muar Malaysia Department of Medicine Clinical Research Center Hospital Kuala Lumpur Kuala Lumpur Malaysia

Background: The size of Hamstring autograft of less than 8.0 mm in ACL re-construction is one of the key factors that may contribute to the failure of the graft. In this study, we are going to assess the correlation of pre-operative MRI measurement of Hamstring tendons with the intra-operative ACL graft. Thus, it may help surgeons to anticipate the needs for graft augmentation should the final graft size be smaller than expected. Methods: We retrospectively re-viewed 41 cases of ACL reconstructions in which MRI were done pre-operatively, in Hospital pakar Sultanah Fatimah (HPSF), Muar between January 2019 until December 2022. MRI measurements of Hamstring tendon (semitendinosus: ST, and gracilis) were done by a radiologist, and the in-tra-operative notes were reviewed for the final graft size. Pearson’s correlation coefficients were calculated to determine the relationship between graft size and tendon cross-sectional area. The intraclass correlation (ICC) by using two-way mixed model with type consistency, the reliability based on average measure was 0.41 (95% CI: ?0.10, 0.69). A p-value < 0.05 was considered sig-nificant. Results: Of 105 patients with primary ACL reconstruction done be-tween January 2019 to December 2022, only 41 patients were included in this study. There was fair correlation (p = 0.048) between pre-operative MRI measurement and the graft diameter intra-operatively. The mean of 17.0 mm of combined diameter of ST and gracilis tendon in MRI may results in graft diameter of 8.3 mm. There was also positive correlation between patients’ height and the intra-operative graft size. Conclusion: These results showed good correlation between pre-operative MRI measurement of Hamstring ten-don size with the intra-operative graft size, hence it is a reliable tool to predict the Hamstring autograft size in ACL reconstruction.

关键词： ACL Reconstruction Pre-Operative MRI Hamstring Tendon

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Efferent limb of gastrojejunostomy obstruction by a whole okra phytobezoar:Case report and brief review

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World Journal of Gastrointestinal Endoscopy 2012年第5期4卷 197-200页

作者： Thant Zin Myat Maw Dinker Ramananda Pai Rosaini Binti Paijan Myo Kyi Department of Surgery Melaka Manipal Medical College75150 MelakaMalaysia Department of Surgery Hospital Pakar Sultanah FatimahMuar JohorMalaysia

A phytobezoar is one of the intraluminal causes of gastric outlet obstruction,especially in patients with previous gastric surgery and/or gastric motility *** the proton pump inhibitor era,vagotomy,pyloroplasty,gastrectomy and gastrojejunostomy were commonly performed procedures in peptic ulcer *** of the sequelae of gastrojejunostomy is phytobezoar ***,a bezoar causing gastric outlet obstruction is rare even with giant gastric *** report a rare case of gastric outlet obstruction due to a phytobezoar obstructing the efferent limb of the gastrojejunostomy *** phytobezoar which consisted of a whole piece of okra(lady finger vegetable) was successfully removed by endoscopic *** the best of our knowledge,this is the first case of okra bezoar-related gastrojejunostomy efferent limb obstruction reported in the literature.

关键词： Efferent limb obstruction Gastrojejunostomy Okra phytobezoar

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