检索结果-南通市图书馆

5-Bromo-2'-deoxyuridine labeling:historical perspectives,factors infiuencing the detection,toxicity,and its implications in the neurogenesis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第2期19卷 302-308页

作者： Joaquín Martí-Clúa unidad de Citología e Histología.Departament de Biologia Cel·lular de Fisiologia i d’Immunologia.Facultad de Biociencias.Institut de Neurociències.Universidad Autónoma de Barcelona08193 BellaterraBarcelonaSpain

The halopyrimidine 5-bromo-2′-deoxyuridine(BrdU)is an exogenous marker of DNA *** the introduction of monoclonal antibodies against BrdU,an increasing number of methodologies have been used for the immunodetection of this synthesized bromine-tagged base analogue into replicating *** labeling is widely used for identifying neuron precursors and following their fate during the embryonic,perinatal,and adult neurogenesis in a variety of vertebrate species including birds,reptiles,and *** to BrdU toxicity,its incorporation into replicating DNA presents adverse consequences on the generation,survival,and settled patterns of *** may lead to false results and misinterpretation in the identification of proliferative *** this review,I will indicate the detrimental effects of this nucleoside during the development of the central nervous system,as well as the reliability of BrdU labeling to detect proliferating ***,it will show factors influencing BrdU immunodetection and the contribution of this nucleoside to the study of prenatal,perinatal,and adult *** adult neurogenesis will also be *** is my hope that this review serves as a reference for those researchers who focused on detecting cells that are in the synthetic phase of the cell cycle.

关键词： 5-bromo-2′-deoxyuridine adult neurogenesis human adult neurogenesis labeling pitfalls prenatal neurogenesis proliferation S-phase suturing S-phase toxicity

Long-term effects of gestational diabetes mellitus on the pancreas of female mouse offspring

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Diabetes 2024年第4期15卷 758-768页

作者： Enriqueta Muñoz-Islas Edgar David Santiago-SanMartin Eduardo Mendoza-Sánchez Héctor Fabián Torres-Rodríguez Laura Yanneth Ramírez-Quintanilla Christopher Michael Peters Juan Miguel Jiménez-Andrade unidad Académica Multidisciplinaria Reynosa-Aztlán Universidad Autónoma de TamaulipasReynosa 88740TamaulipasMexico Department of Anesthesiology Wake Forest University School of MedicineWinston SalemNC 27101United States

BACKGROUND Prolonged fetal exposure to hyperglycemia may increase the risk of developing abnormal glucose metabolism and type-2 diabetes during childhood,adolescence,and adulthood;however,the mechanisms by which gestational diabetes mellitus(GDM)predisposes offspring to metabolic disorders remain *** To quantify the nerve axons,macrophages,and vasculature in the pancreas from adult offspring born from mouse dams with *** GDM was induced by *** of streptozotocin(STZ)in ICR mouse *** 12 wk old,fasting blood glucose levels were determined in *** 15 wk old,female offspring born from dams with and without GDM were sacrificed and pancreata were processed for *** quantified the density of sensory[calcitonin gene-related peptide(CGRP)]and tyrosine hydroxylase(TH)axons,blood vessels(endomucin),and macro-phages(CD68)in the splenic pancreas using confocal *** Offspring mice born from STZ-treated dams had similar body weight and blood glucose values compared to offspring born from vehicle-treated ***,the density of CGRP+and TH+axons,endomucin+blood vessels,and CD68+macrophages in the exocrine pancreas was significantly greater in offspring from mothers with GDM vs control ***,the microvasculature in the islets was significantly greater,but not the number of macrophages within the islets of offspring born from dams with GDM compared to control *** GDM induces neuronal,vascular,and inflammatory changes in the pancreas of adult progeny,which may partially explain the higher propensity for offspring of mothers with GDM to develop metabolic diseases.

关键词： Gestational diabetes mellitus Immunohistochemistry Confocal microscopy Pancreas Offspring

Chromomagnetic dipole moments of light quarks in the bestest little Higgs model

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Physics C 2024年第10期48卷 125-136页

作者： T.Cisneros-Pérez E.Cruz-Albaro A.Y.Ojeda-Castañeda S.E.Solís-Núñez unidad Académica de Ciencias Químicas Universidad Autónoma de ZacatecasApartado Postal C-58598060 ZacatecasMexico Facultad de Física Universidad Autónoma de ZacatecasApartado Postal C-58098060 ZacatecasMexico

In this study,we investigated the anomalous Chromomagnetic Dipole Moment(CMDM),denoted as μ_(q)^(BLHM),of light quarks q=(u,c,d,s,b) within the framework of the Bestest Little Higgs Model(BLHM)as an extension of the Standard Model(SM).Our investigation encompassed novel interactions among the light quarks,heavy quark B,and heavy bosons (W'^(±),H^(±),φ^(±),η^(±))incorporating the extended Cabibbo-Kobayashi-Maskawa(CKM)matrix characteristic of the *** thoroughly explored the permissible parameter space,yielding a spectrum of CMDM values ranging from 10^(-10) to 10^(-3).

关键词： beyond standard model flavor physics chromomagnetic dipole light quarks

Morphometry and Mineral Content in the Seeds and Soil of Two Species of Argemone L.(Papaveraceae)in the Central Part of the Chihuahuan Desert

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2024年第2期93卷 371-386页

作者： Perla Patricia Ochoa-García Jaime Sánchez-Salas Ricardo Trejo-Calzada Jesús JosafathQuezada-Rivera Fabián García-González unidad Regional Universitaria de ZonasÁridas BermejilloUniversidad Autónoma ChapingoDurango35230México Facultad de Ciencias Biológicas Universidad Juárez del Estado de DurangoGómez PalacioDurango35010México

The genus Argemone L.(Papaveraceae)is found widely distributed in Mexico’s Chihuahuan Desert(CD).Some species of this genus are of phytochemical or ethnobotanical *** are inedible plants considered as *** date they have not been broadly studied;thus,their ecology is,to our knowledge,*** present work was centered around carrying out a morphometric analysis and the determination of minerals in the soil and seeds of the wild populations of Argemone at sites belonging to two ecoregions of the CD in *** April 2021 and April 2022,seeds of Argemone spp.,and soil samples were collected at 10 sites of the *** seeds were selected under a randomized design,and weight,length,diameter,thickness,buoyancy,and mineral content were *** soil samples were obtained under the Mexican regulation NOM-021-RECNAT-2000,and determinations of mineral content,electrical conductivity,apparent density,and soluble anions were *** information obtained was grouped by variable,species,and place of *** statistical tests consisted of an ANOVA,Tukey means tests considering p≤0.05,and a Principal Components *** pleiacantha exhibited differences in terms of weight(F=54.79,p=0.001),length(F=90.83,p=0.001),thickness(F=104.89,p=0.001),and diameter(F=155.82,p=0.001),and the differences in Argemone mexicana were in weight(F=46.71,p=0.001),thickness(F=187.49,p=0.001),length(F=191.56,p=0.001),and diameter(F=215.83,p=0.001).The evaluated seeds reached their maximal imbibition velocity at 24 h of *** content of the micro-and macro-nutrients analyzed in the seeds and soil suggest a tight relation with the morphometric characteristics of the seeds.

关键词： Micronutrients agricultural area semiarid zone ecoregion buoyancy

Breast cancer immunotherapy:Realities and advances

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cancer Innovation 2024年第5期3卷 23-36页

作者： Aixa Medina Jeismar Carballo Eglys González‐Marcano Isaac Blanca Ana F.Convit Jacinto Convit World Organization Inc. Pompano BeachFloridaUSA Facultad de Medicina Universidad Central de VenezuelaCaracasVenezuela unidad Experimental de Inmunoterapia Fundación Jacinto ConvitCaracasVenezuela

Breast cancer(BC)is the most common malignant tumor and the main cause of death in women *** increased knowledge regarding tumor escape mechanisms and advances in immunology,many new antitumor strategies such as nonspecific immunotherapies,monoclonal antibodies,anticancer vaccines,and oncolytic viruses,among others,make immunotherapy a promising approach for the treatment of ***,these approaches still require meticulous assessment and readjustment as resistance and modest response rates remain important *** this article,we aim to summarize the most recent data available in BC immunotherapy to include the results of ongoing clinical trials and approved therapies used as monotherapies or in combination with conventional treatments.

关键词： breast cancer immunotherapy metastatic disease therapy combination tumor microenvironment

Highly divergent sympatric lineages of Leptotila verreauxi(Aves:Columbidae)suggest a secondary contact area in the Isthmus of Tehuantepec,Mexico

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Avian Research 2024年第1期15卷 12-21页

作者： Orlando J.Espinosa-Chávez Adolfo G.Navarro-Sigüenza Hernando Rodríguez-Correa Luis A.Sánchez-González Museo de Zoología“Alfonso L.Herrera” Depto.de Biología EvolutivaFacultad de CienciasUniversidad Nacional Autonoma de MexicoApdo.Postal 70-399CP 04510Ciudad de MexicoMexico Posgrado en Ciencias Biologicas Unidad de PosgradoEdificio D1◦PisoCircuito de PosgradosCiudad UniversitariaUniversidad Nacional Autonoma de MexicoCoyoacanCP 04510CDMXMexico Escuela Nacional de Estudios Superiores unidad Morelia Universidad Nacional Autonoma de Mexico.Antigua Carretera a Patzcuaro No.8701 Col.Ex Hacienda de San Jose de la HuertaC.P.58190MoreliaMichoacanMexico

Due to a complex geological and biotic history,the Isthmus of Tehuantepec(IT),has been long recognized as a driver for the evolutionary divergence of numerous lowland and highland *** distributed in the lowlands of the American continent,the White-Tipped Dove(Leptotila verreauxi)is a polytypic species with 13 recognized *** of these have been recorded in Mexico,and the distribution of three abuts at the IT,suggesting a contact *** estimate phylogenetic patterns,divergence times and genetic differentiation,we examined two mt DNA(ND2 and COI)and one n DNA(β-fibint 7)*** also used correlative ecological niche models(ENM)to assess whether ecological differences across the IT may have acted as a biogeographical *** estimated paleodistributions during the Middle Holocene,Last Glacial Maximum and Last Interglacial,to evaluate the influence of climate changes on the distribution and demographic *** results showed genetically distinct lineages that diverged approximately 2.5 million years *** and ecological factors may have played a dual role in promoting differentiation,but also in the formation of a secondary contact zone in the southern *** ecological niche comparisons indicated that the ecological niche of sympatric lineages at the IT are not identical,suggesting niches divergence;in addition,environmental niche models across the region indicated no abrupt biogeographic barriers,but the presence of regions with low *** results suggest that genetic differentiation originated by a vicariant event probably related to environmental factors,favored the evolution of different ecological ***,the absence of a biogeographic barrier but the presence of less suitable areas in the contact regions,suggest that secondary contact zones may be also maintained by climatic factors for the eastern group,but also by biotic interactions for the western group.

关键词： Biogeographical boundary Humid and dry forests Isthmus of Tehuantepec Phylogeography Secondary contact

Integrative Analysis of Transcriptome and Phenolic Compounds Profile Provides Insights into the Quality of Soursop (Annona muricata L.) Fruit

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2024年第7期93卷 1717-1732页

作者： Yolotzin Apatzingán Palomino-Hermosillo Ángel Elpidio Díaz-Jasso Rosendo Balois-Morales Verónica AlhelíOchoa-Jiménez Pedro Ulises Bautista-Rosales Guillermo Berumen-Varela unidad de Tecnología de Alimentos-Secretaría de Investigación y Posgrado Universidad Autónoma de Nayarit.Ciudad de la Cultura SNTepic63000NayaritMéxico Programa Académico de Biología Unidad Académica de AgriculturaUniversidad Autónoma de NayaritKm.9.Carretera Tepic-CompostelaXalisco63780NayaritMéxico Estancias Posdoctorales-Consejo Nacional de Humanidades Ciencias y TecnologíasCoordinación de Apoyos a Becarios e InvestigadoresDirección de PosgradoCiudad de México03940México

Soursop(Annona muricata L.)is a tropical fruit highly valued for its uniqueﬂavor,nutritional value,and health-promoting *** ripening process of soursop involves complex changes in gene expression and metabo-lite accumulation,which have been studied using various omics *** analysis has provided insights into the regulation of key genes involved in ripening,while metabolic compound analysis has revealed the presence of numerous bioactive compounds with potential health beneﬁ***,the integration of transcrip-tome and metabolite compound data has not been extensively explored in ***,in this paper,we present a comprehensive analysis of the transcriptome and phenolic compound proﬁles of soursop during *** integration analysis showed that the genes and phenolic compounds were mainly involved in the starch and sucrose metabolism pathways during soursop ***,the phenolic compounds Kaempferol 3-Q-galactoside,Procyanidin C1,Procyanidin trimmer C1,and m-Coumaric,as well as the genes Ubiquitin-like protein 5(UBL5_ARATH),ATP-dependent zinc metalloprotease FTSH8(FTSH8_ORYSJ),Zinc transporter 4(ZIP4_AR-ATH),Thioredoxin-like 3-1(TRL31_ORYSJ),Mitogen-activated protein kinase YODA(YODA_ARATH),R-man-delonitrile lyase-like(MGL_ARATH),26s protease regulatory subunit 6A homolog(PRS6_SOLLC),Cytochrome P45072A13(C7A13ARATH),Cytochrome P45084A1(C84A1_ARATH)and Homoserine O-trans-acetylase(MET2-ORYSJ)were correlated and differentially accumulated and expressed,*** study provides new insights into the molecular mechanisms underlying soursop ripening and may contribute to the development of strategies for improving the nutritional quality and shelf life of this important fruit.

关键词： Bioinformatics data integration genes metabolites postharvest storage ripening

The relationship between DNA fragmentation and the intensity of morphologically abnormal human spermatozoa

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian pacific Journal of Reproduction 2024年第1期13卷 22-27页

作者： Mercedes González-Martínez Pascual Sánchez-Martín Carmen López-Fernández Stephen D.Johnston Jaime Gosálvez Clínica Ginemed Unidad de ReproducciónSevillaSpain Department of Biology Universidad Autónoma de MadridCantoblancoMadridSpain School of Environment The University of QueenslandGattonAustralia School of Veterinary Science The University of QueenslandGattonAustralia

Objective:To determine the relationship between teratozoospermia and sperm DNA fragmentation(SDF)in the human ***:This retrospective study included 100 normozoospermic men as a control cohort(abnormal forms>14%),210 patients with a high level of abnormal forms(≤4%)and 65 patients presenting with a moderate level of abnormal forms(>4%to≤14%)based on the World Health Organization *** morphology was assessed using bright field *** DNA fragmentation was assessed using the sperm chromatin dispersion ***-parametric analyses were conducted to examine the relationship between abnormal sperm morphology and sperm DNA fragmentation;receiver operating characteristic(ROC)analyses were conducted to assess sensitivity and specificity of this ***:A correlation analysis revealed that the higher the proportion of abnormal spermatozoa in the ejaculate,the higher the level of SDF(Spearman's Rho=-0.230;P<0.001).Significant differences in the proportion of SDF were found when all cohorts were compared(P<0.001);these significant differences were also retained when the different cohorts were compared *** analysis showed a moderate but significant predictive value for SDF to differentiate patients with different levels of ***:Although analysis of a more continuous range of values for teratozoospermia would help further clarify any causal relationship with SDF,there is clearly a synergistic or coincident affiliation between these variables that needs to be acknowledged by the clinician when interpreting the spermiogram.

关键词： Teratozoospermia Sperm morphology Sperm DNA fragmentation Male factor Human reproduction Human fertility

Effect of Ultraviolet Radiation on Hsp70 Protein Expression in HaCaT Cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Nuclear Science and Technology 2024年第1期14卷 59-71页

作者： Sergio Hugo Sánchez Rodríguez Jesús Rodríguez Vergil Manuel Venancio Muñoz Juárez Kevin Said Ramírez Dávila Luis Martín García Ortiz Germán Flores Cortés Luz Elena Vidales Rodríguez Jesús Adrián López David Alejandro García López Laboratorio de Biología Celular y Neurobiología Unidad Académica de Ciencias Biológicas Universidad Autónoma de Zacatecas Zacatecas México Laboatorio de Bacterias y Hongos Filamentosos Unidad Académica de Ciencias Biológicas Universidad Autónoma de Zacatecas Zacatecas México Laboratorio de MicroRNAs Unidad Académica de Ciencias Biológicas Universidad Autónoma de Zacatecas Zacatecas México

Ultraviolet radiation by its wavelength is divided into: UVA, UVB and UVC. Only UVA and UVB manage to penetrate the ozone layer, but due to anthropological activities, all of them are capable of interacting with humans to a greater or lesser extent, and can generate adverse effects such as cellular stress when interacting with intra-and extracellular biomolecules. The skin is the first organ in contact with UV radiation, and the stress it generates can be analyzed by the expression of a bioindicator of cellular damage such as Hsp70. Therefore, the objective of the project was: to determine the effect of UVA, UVB and UVC radiation on HaCaT epithelial cells, by analyzing the expression of Hsp70. Materials and methods: HaCaT cells were cultured in vitro, which were irradiated with UVA, UVB and UVC light at different doses, to subsequently determine the degree of Hsp70 expression by Immunodetection by PAGE-SDS and Western Blot. Results: Basal expression of Hsp70 was observed in no irradiated HaCaT cells. When HaCaT cells were irradiated with UVA, UVB, UVC, an increase in this Hsp70 protein was observed. With UVA, a higher degree of expression was observed at a time of 30 minutes of irradiation. With UVB the highest expression shifted to a time of 20 minutes. With UVC, overexpression was observed after 10 minutes. Conclusion: UV radiation generates cellular stress on HaCaT cells, evaluated by the stress bioindicator Hsp70. According to the wavelength of UV radiation, those that have a shorter wavelength have a greater potential for cellular damage, such as UVC.

关键词： Ultraviolet A Light (UVA) Ultraviolet B Light (UVB) Ultraviolet C Light (UVC) Heat Shock Protein 70 (Hsp70) HaCaT