The relationship between DNA fragmentation and the intensity of morphologically abnormal human spermatozoa
作者机构:Clínica GinemedUnidad de ReproducciónSevillaSpain Department of BiologyUniversidad Autónoma de MadridCantoblancoMadridSpain School of EnvironmentThe University of QueenslandGattonAustralia School of Veterinary ScienceThe University of QueenslandGattonAustralia
出 版 物:《Asian pacific Journal of Reproduction》 (亚太生殖杂志(英文版))
年 卷 期:2024年第13卷第1期
页 面:22-27页
学科分类:0832[工学-食品科学与工程(可授工学、农学学位)] 08[工学]
主 题:Teratozoospermia Sperm morphology Sperm DNA fragmentation Male factor Human reproduction Human fertility
摘 要:Objective:To determine the relationship between teratozoospermia and sperm DNA fragmentation(SDF)in the human ejaculate.Methods:This retrospective study included 100 normozoospermic men as a control cohort(abnormal forms14%),210 patients with a high level of abnormal forms(≤4%)and 65 patients presenting with a moderate level of abnormal forms(4%to≤14%)based on the World Health Organization definitions.Sperm morphology was assessed using bright field microscopy.Sperm DNA fragmentation was assessed using the sperm chromatin dispersion assay.Non-parametric analyses were conducted to examine the relationship between abnormal sperm morphology and sperm DNA fragmentation;receiver operating characteristic(ROC)analyses were conducted to assess sensitivity and specificity of this relationship.Results:A correlation analysis revealed that the higher the proportion of abnormal spermatozoa in the ejaculate,the higher the level of SDF(Spearman s Rho=-0.230;P0.001).Significant differences in the proportion of SDF were found when all cohorts were compared(P0.001);these significant differences were also retained when the different cohorts were compared pairwise.ROC analysis showed a moderate but significant predictive value for SDF to differentiate patients with different levels of teratozoospemia.Conclusions:Although analysis of a more continuous range of values for teratozoospermia would help further clarify any causal relationship with SDF,there is clearly a synergistic or coincident affiliation between these variables that needs to be acknowledged by the clinician when interpreting the spermiogram.