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Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes

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Light(Science & Applications) 2022年第10期11卷 2271-2275页

作者： Xinzhu Xu Shu Jia Peng Xi Department of biomedical Engineering College of Future TechnologyPeking UniversityBeijing 100871China Wallace H.Coulter Department of biomedical Engineering Georgia Institute of Technology and Emory UniversityAtlantaGeorgia 30332USA uts-sustech joint research centre for Bio-medical materials&devices Department of Biomedical EngineeringCollege of EngineeringSouthern University of Science and TechnologyShenzhenGuangdong 518055China National biomedical Imaging Center Peking UniversityBeijing 100871China

A donut excitation moves around a single molecule with a zigzag configuration lattice by *** a method implemented in scanning fluorescence microscopy simplifies the conventional MINFLUX *** of hollow zero-intensity excitation,single-pixel detection,time-correlated single photon counting,and drift stabilization,the system achieves localization precision and resolution very close to conventional MINFLUX theoretically and *** averaged high-SNR reference,and pixel-registered intensity from a single molecule is essential to reconstruct localization in maximum likelihood *** performance reaching nearly conventional MINFLUX's,the proposed raster-scanning MINFLUX can inspire researchers expertized in STED or confocal setup to quickly transform to MINFLUX and develop for further exploring on bio-specimens or optical applications.

关键词： scanning excitation stabilization

Viral inactivation by light

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eLight 2022年第1期2卷 237-254页

作者： Mohammad Sadraeian Le Zhang Farzaneh Aavani Esmaeil Biazar Dayong Jin Present Address:Institute for biomedical materials and devices(IBMD) Faculty of ScienceUniversity of Technology SydneySydneyNSW 2007Australia Department of Oral and Maxillofacial Surgery Division of Regenerative Orofacial MedicineUniversity Hospital Hamburg-Eppendorf20251 HamburgGermany Department of biomedical Engineering Islamic Azad UniversityTonekabon BranchTonekabonIran uts‑sustech joint research centre for biomedical materials&devices Department of Biomedical EngineeringCollege of EngineeringSouthern University of Science and TechnologyShenzhenGuangdongChina

Nowadays,viral infections are one of the greatest challenges for medical sciences and human *** antiviral compounds and chemical inactivation remain inadequate,physical approaches based on irradiation provide new potentials for prevention and treatment of viral infections,without the risk of drug resistance and other unwanted side *** across the electromagnetic spectrum can inactivate the virions using ionizing and non-ionizing *** review highlights the anti-viral utility of radiant methods from the aspects of ionizing radiation,including high energy ultraviolet,gamma ray,X-ray,and neutron,and non-ionizing photo-inactivation,including lasers and blue light.

关键词： Antiviral therapy Enveloped virus Photo-inactivation Ionizing radiation Nuclear radiations

Deep learning enhanced NIR-Ⅱ volumetric imaging of whole mice vasculature

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Opto-Electronic Advances 2023年第4期6卷 6-14页

作者： Sitong Wu Zhichao Yang Chenguang Ma Xun Zhang Chao Mi Jiajia Zhou Zhiyong Guo Dayong Jin uts-sustech joint research centre for biomedical materials&devices Department of Biomedical EngineeringSouthern University of Science and TechnologyShenzhen 518055China Institute for biomedical materials&devices Faculty of ScienceUniversity of Technology SydneyUltimoNew South Wales 2007Australia Guangdong Provincial Key Laboratory of Advanced Biomaterials Southern University of Science and TechnologyShenzhen 518055China

Fluorescence imaging through the second near-infrared window(NIR-Ⅱ,1000–1700 nm) allows in-depth ***, current imaging systems use wide-field illumination and can only provide low-contrast 2D information, without depth resolution. Here, we systematically apply a light-sheet illumination, a time-gated detection, and a deep-learning algorithm to yield high-contrast high-resolution volumetric images. To achieve a large Fo V(field of view) and minimize the scattering effect, we generate a light sheet as thin as 100.5 μm with a Rayleigh length of 8 mm to yield an axial resolution of 220 μm. To further suppress the background, we time-gate to only detect long lifetime luminescence achieving a high contrast of up to 0.45 Icontrast. To enhance the resolution, we develop an algorithm based on profile protrusions detection and a deep neural network and distinguish vasculature from a low-contrast area of 0.07 Icontrast to resolve the 100μm small vessels. The system can rapidly scan a volume of view of 75 × 55 × 20 mm3and collect 750 images within 6mins. By adding a scattering-based modality to acquire the 3D surface profile of the mice skin, we reveal the whole volumetric vasculature network with clear depth resolution within more than 1 mm from the skin. High-contrast large-scale 3D animal imaging helps us expand a new dimension in NIR-Ⅱ imaging.

关键词： NIR-Ⅱfluorescence time-gated light sheet illumination deep learning vessel enhancement 3D imaging

Two-photon MINFLUX with doubled localization precision

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eLight 2022年第1期2卷 58-67页

作者： Kun Zhao Xinzhu Xu Wei Ren Dayong Jin Peng Xi Department of biomedical Engineering College of Future TechnologyPeking UniversityBeijing 100871China Wallace H.Coulter Department of biomedical Engineering Georgia Institute of Technology and Emory UniversityAtlanta30332 GeorgiaUSA uts‑sustech joint research centre for biomedical materials&devices Department of Biomedical EngineeringCollege of EngineeringSouthern University of Science and TechnologyShenzhenGuangdong 518055China National biomedical Imaging Center Peking UniversityBeijing 100871China

Achieving localization with molecular precision has been of great interest for extending fluorescence microscopy to *** pioneers this transition through point spread function(PSF)engineering,yet its performance is primarily limited by the signal-to-background *** we demonstrate theoretically that two-photon MINFLUX(2p-MINFLUX)could double its localization precision through PSF engineering by nonlinear ***ér-Rao Bound(CRB)is studied as the maximum localization precision,and CRB of two-photon MINFLUX is halved compared to single-photon MINFLUX(1p-MINFLUX)in all three ***,in order to achieve same localization precision with 1p-MINFLUX,2p-MINFLUX requires only 1/4 of fluorescence *** simultaneous two-photon excitation of multiple fluorophore species,2p-MINFLUX may have the potential for registration-free nanoscopy and multicolor tracking.

关键词： Super-resolution microscopy Single-molecule localization MINFLUX Two-photon microscopy

Polarization modulation with optical lock-in detection reveals universal fluorescence anisotropy of subcellular structures in live cells

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Light(Science & Applications) 2022年第1期11卷 50-62页

作者： Meiling Guan Miaoyan Wang Karl Zhanghao Xu Zhang Meiqi Li Wenhui Liu Jing Niu Xusan Yang Long Chen Zhenli Jing Micheal Q.Zhang Dayong Jin Peng Xi Juntao Gao Department of biomedical Engineering College of Future TechnologyPeking UniversityBeijing 100871China uts-sustech joint research centre for biomedical materials&devices Department of Biomedical EngineeringCollege of EngineeringSouthern University of Science and TechnologyShenzhenGuangdongChina MOE Key Laboratory of Bioinformatics Bioinformatics DivisionCenter for Synthetic&Systems BiologyBNRistBeijingChina Center for Synthetic&Systems Biology Department of AutomationTsinghua UniversityBeijing 100084China Beijing Institute of Collaborative Innovation Beijing 100094China Department of Biological Sciences and Center for System Biology The University of Texas at DallasRichardson 75080USA School of Medical Sciences Tsinghua UniversityBeijing 100084China Institute for biomedical materials and devices(IBMD) Faculty of ScienceUniversity of Technology SydneySydneyNSW 2007Australia National biomedical Imaging Center Peking UniversityBeijing 100871China

The orientation of fluorophores can reveal crucial information about the structure and dynamics of their associated subcellular *** significant progress in super-resolution,fluorescence polarization microscopy remains limited to unique samples with relatively strong polarization modulation and not applicable to the weak polarization signals in samples due to the excessive background *** we apply optical lock-in detection to amplify the weak polarization modulation with *** novel technique,termed optical lock-in detection super-resolution dipole orientation mapping(OLID-SDOM),could achieve a maximum of 100 frames per second and rapid extraction of 2D orientation,and distinguish distance up to 50 nm,making it suitable for monitoring structural dynamics concerning orientation changes in ***-SDOM was employed to explore the universal anisotropy of a large variety of GFP-tagged subcellular organelles,including mitochondria,lysosome,Golgi,endosome,*** found that OUF(Orientation Uniformity Factor)of OLID-SDOM can be specific for different subcellular organelles,indicating that the anisotropy was related to the function of the organelles,and OUF can potentially be an indicator to distinguish normal and abnormal cells(even cancer cells).Furthermore,dual-color super-resolution OLID-SDOM imaging of lysosomes and actins demonstrates its potential in studying dynamic molecular *** subtle anisotropy changes of expanding and shrinking dendritic spines in live neurons were observed with real-time *** previously unobservable fluorescence anisotropy in various samples and indicating their underlying dynamic molecular structural changes,OLID-SDOM expands the toolkit for live cell research.

关键词： anisotropy Polarization concerning

Tn5-FISH,a novel cytogenetic method to image chromatin interactions with sub-kilobase resolution

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Journal of Genetics and Genomics 2020年第12期47卷 727-734页

作者： Jing Niu Xu Zhang Li Guipeng Pixi Yan Qing Yan Qionghai Dai Dayong Jin Xiaohua Shen Jichang Wang Michael QZhang Juntao Gao School of Medicine Tsinghua UniversityBeijing 100084China Department of Automation Tsinghua UniversityBeijing 100084China Beijing Institute of Collaborative Innovation Beijing 100094China Medi-X Institute SUSTech Academy for Advanced Interdisciplinary StudiesSouthern University of Science and TechnologyShenzhen 518055China Department of Biology Southern University of Science and TechnologyShenzhen 518055China MOE Key Laboratory of Bioinformatics Bioinformatics DivisionBNRistCenter for Synthetic&Systems BiologyTsinghua UniversityBeijing 100084China Institute for biomedical materials and devices(IBMD) Faculty of ScienceUniversity of Technology SydneyNSW 2007Australia uts-sustech joint research centre for biomedical materials and devices Department of Biomedical EngineeringSouthern University of Science and TechnologyShenzhen 518055China Key Laboratory for Stem Cells and Tissue Engineering(Sun Yat-sen University) Ministry of EducationGuangzhou 510080China Department of histology and embryology Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080China Department of Biological Sciences Center for Systems BiologyThe University of TexasDallasTX 75080-3021USA

There is an increasing interest in understanding how three-dimensional(3D)organization of the genome is *** strategies have been employed to identify genome-wide chromatin ***,due to current limitations in resolving genomic contacts,visualization and validation of these genomic loci with sub-kilobase resolution remain unsolved to ***,we describe Tn5 transposase-based Fluorescencein situhybridization(Tn5-FISH),a PCR-based,cost-effective imaging method,which can co-localize the genomic loci with sub-kilobase resolution,dissect genome architecture,and verify chromatin interactions detected by chromatin configuration capture(3C)-derived *** validate this method,short-range interactions in keratin-encoding gene(KRT)locus in topologically associated domain(TAD)were imaged by triple-color Tn5-FISH,indicating that Tn5-FISH is very useful to verify short-range chromatin interactions inside the contact domain and ***,Tn5-FISH can be a powerful molecular tool for the clinical detection of cytogenetic changes in numerous genetic diseases such as cancers.

关键词： Fluorescencein situhybridization Chromatin interaction Cellular imaging Super resolution

Upconversion nanoparticles for super-resolution quantification of single small extracellular vesicles

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eLight 2022年第1期2卷 262-273页

作者： Guan Huang Yongtao Liu Dejiang Wang Ying Zhu Shihui Wen Juanfang Ruan Dayong Jin Institute for biomedical materials and devices(IBMD) Faculty of ScienceUniversity of Technology SydneySydneyNSW 2007Australia Smart Computational Imaging Laboratory(SCILab) School of Electronic and Optical EngineeringNanjing University of Science and TechnologyNanjingJiangsu 210094China School of biomedical Engineering Faculty of Engineering and Information TechnologyUniversity of Technology SydneySydneyNSW 2007Australia School of Clinical Medicine Faculty of Medicine&HealthUNSW SydneySydneyNSW 2052Australia Electron Microscope Unit UNSW SydneySydneyNSW 2052Australia ARC research Hub for Integrated Device for End‑User Analysis at Low Levels Faculty of ScienceUniversity of Technology SydneySydneyNSW 2007Australia uts‑sustech joint research centre for biomedical materials and devices Department of Biomedical EngineeringSouthern University of Science and TechnologyShenzhenGuangdong 518055China

Although small EVs(sEVs)have been used widely as biomarkers in disease diagnosis,their heterogeneity at single EV level has rarely been *** is because high-resolution characterization of sEV presents a major challenge,as their sizes are below the optical diffraction ***,we report that upconversion nanoparticles(UCNPs)can be used for super-resolution profiling the molecular heterogeneity of *** show that Er3+-doped UCNPs has better brightness and Tm3+-doped UCNPs resulting in better resolution beyond diffraction *** an orthogonal experimental design,the specific targeting of UCNPs to the tumour epitope on single EV has been cross validated,resulting in the Pearson’s R-value of 0.83 for large EVs and~65%co-localization double-positive spots for ***,super-resolution nanoscopy can distinguish adjacent UCNPs on single sEV with a resolution of as high as 41.9 *** decreasing the size of UCNPs from 40 to 27 nm and 18 nm,we observed that the maximum UCNPs number on single sEV increased from 3 to 9 and 21,*** work suggests the great potentials of UCNPs approach“digitally”quantify the surface antigens on single EVs,therefore providing a solution to monitor the EV heterogeneity changes along with the tumour progression progress.

关键词： Extracellular vesicles Upconversion nanoparticles Super-resolution Liquid biopsy

Laterally swept light-sheet microscopy enhanced by pixel reassignment for photon-efficient volumetric imaging

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Advanced Photonics Nexus 2023年第1期2卷 6-14页

作者： Liang Qiao Hongjin Li Suyi Zhong Xinzhu Xu Fei Su Xi Peng Dayong Jin Karl Zhanghao Southern University of Science and Technology College of EngineeringUTS-SUSTech Joint Research Centre for Biomedical Materials and DevicesDepartment of Biomedical EngineeringShenzhenChina City University of Hong Kong Department of Biomedical EngineeringHong KongChina Peking University College of Future TechnologyDepartment of Biomedical EngineeringBeijingChina University of Technology Sydney Institute for Biomedical Materials and Devices(IBMD)Faculty of ScienceSydneyAustralia

In light-sheet fluorescence microscopy,the axial resolution and field of view are mutually *** swept light-sheet microscopy(ASLM)can decouple the trade-off,but the confocal detection scheme using a rolling shutter also rejects fluorescence signals from the specimen in the field of interest,which sacrifices the photon ***,we report a laterally swept light-sheet microscopy(LSLM)scheme in which the focused beam is first scanned along the axial direction and subsequently laterally swept with the rolling *** show that LSLM can obtain a higher photon efficiency when similar axial resolution and field of view can be ***,based on the principle of image scanning microscopy,applying the pixel reassignment to the LSLM images,hereby named iLSLM,improves the optical *** simulation and experimental results demonstrate the higher photon efficiency with similar axial resolution and optical *** proposed scheme is suitable for volumetric imaging of specimens that are susceptible to photobleaching or phototoxicity.

关键词： light-sheet fluorescence microscopy image scanning microscopy volumetric imaging pixel reassignment

Light-Driven Ion Transport in Nanofluidic devices:Photochemical,Photoelectric,and Photothermal Effects

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CCS Chemistry 2022年第1期4卷 54-65页

作者： Kai Xiao Oliver G.Schmidt Department of biomedical Engineering Southern University of Science and Technology(SUSTech)Shenzhen 518055 Center for materials Architectures and Integration of Nanomembranes(MAIN)Chemnitz University of TechnologyChemnitz 09126 Institute for Integrative Nanosciences Leibniz IFW DresdenDresden 01069

Light-driven ion transport in nanofluidic devices is a phenomenon where ions move unidirectionally by consuming optical energy,either from low concentration to high concentration or vice *** light-driven unidirectional ion transport offers intriguing application potential in desalination and ion separation,osmosis energy harvesting,and ionic machines benefiting from the remote noncontact light ***,we review recent progress in nanofluidic-based light-driven ion transport systems and emphasize similarities and differences in the three underlying working principles based on photochemical,photoelectric,and photothermal *** current challenges and future developments of light-driven ion transport in nanofluidic devices are *** believed that this article encourages further innovation in this exciting and emerging research field.

关键词： nanofluidic artificial ion pump nanochannel ion transport photoelectric effect