Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes

作     者：Xinzhu Xu Shu Jia Peng Xi Xinzhu Xu;Shu Jia;Peng Xi

作者机构：Department of Biomedical EngineeringCollege of Future TechnologyPeking UniversityBeijing 100871China Wallace H.Coulter Department of Biomedical EngineeringGeorgia Institute of Technology and Emory UniversityAtlantaGeorgia 30332USA UTS-SUStech Joint Research Centre for Bio-medical Materials&DevicesDepartment of Biomedical EngineeringCollege of EngineeringSouthern University of Science and TechnologyShenzhenGuangdong 518055China National Biomedical Imaging CenterPeking UniversityBeijing 100871China 

出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)

年 卷 期：2022年第11卷第10期

页      面：2271-2275页

核心收录：

学科分类：07[理学] 0702[理学-物理学] 

主　　题：scanning excitation stabilization 

摘      要：A donut excitation moves around a single molecule with a zigzag configuration lattice by *** a method implemented in scanning fluorescence microscopy simplifies the conventional MINFLUX *** of hollow zero-intensity excitation,single-pixel detection,time-correlated single photon counting,and drift stabilization,the system achieves localization precision and resolution very close to conventional MINFLUX theoretically and *** averaged high-SNR reference,and pixel-registered intensity from a single molecule is essential to reconstruct localization in maximum likelihood *** performance reaching nearly conventional MINFLUX s,the proposed raster-scanning MINFLUX can inspire researchers expertized in STED or confocal setup to quickly transform to MINFLUX and develop for further exploring on bio-specimens or optical applications.