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Insect Science 2016年第4期23卷 621-629页

作者： Qiang Xu Felix D. Guerrero Azhahianambi Palavesam Adalberto A. Perez de Leon Department of Biology Abilene Christian University Abilene TX 79699 USA usda-ars Knipling-Bushland U.S. Livestock Insects Research Laboratory 2700 Fredericksburg Rd. Kerrville TX 78028 USA USDA-ARS Veterinary Pest Genomics Center Kerrville TX 78028 USA

The horn fly, Haematobia irritans, is a serious pest of cattle in North America. The control of horn flies has primarily relied on insecticides. However, the heavy use of insecticides has led to the development of insecticide resistance in horn flies. Novel methods to control horn flies are greatly needed. Transgenic technology is an effective tool to genetically modify insects and may lead to novel methods of pest control based on genomic approaches. Here we report apiggyBac-mediated transformation of the horn fly via electroporation. Transformation with a DsRed fluorescent marker protein coding region was verified by PCR analysis of individual fly bodies and pupal cases and sequencing of PCR products. However, Southern blot analysis failed to indicate the DsRed gene was integrated into the horn fly genome. Thus, the electroporation protocol may have caused the DsRed gene to be integrated into bacterial symbionts of the horn fly.

关键词： DsRed EGFP electroporation Haematobia irritans horn fly transformation transgenic insect

Methoprene application and diet protein supplementation to male melon fly, Bactrocera cucurbitae, modifies female remating behavior

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Insect Science 2014年第5期21卷 637-646页

作者： Ihsan ul Haq Marc J. B. Vreysen P. E. A. Teal Jorge Hendrichs Insect pest Control Laboratory Joint FAO/IAEA Agriculture and Biotechnology Laboratories A-2444 Seibersdorf Austria NationalAgricultural Research Centre Park Road Islamabad 45500 Pakistan center for Medical Agricultural and Veterinary EntomologyUSDA ARS Gainesville Florida 32604 USA Insect pest Control Section Joint FAO/IAEA Division IAEA A-1400 Vienna Austria

Methoprene （an analogue of juvenile hormone） application and feeding on a protein diet is known to enhance male melon fly, Bactrocera cucurbitae Coquillett （Diptera： Tephritidae）, mating success. In this study, we investigated the effect of these treatments on male B. cucurbitae＇s ability to inhibit female remating. While 14-d-old females were fed on protein diet, 6-d-old males were exposed to one of the following treatments：（i） topical application of methoprene and fed on a protein diet; （ii） no methoprene but fed on a protein diet; （iii） methoprene and sugar-fed only; and （iv） sugar-fed, 14-d-old males acted as controls. Treatments had no effect on a male＇s ability to depress the female remating receptivity in comparison to the control. Females mated with protein-deprived males showed higher remating receptivity than females first mated with protein-fed males. Methoprene and protein diet interaction had a positive effect on male mating success during the first and second mating of females. Significantly more females first mated with sugar-fed males remated with protein-fed males and females first mated with methoprene treated and protein-fed males were more likely to remate with similarly treated males. Females mating latency （time to start mating） was significantly shorter with protein-fed males, and mating duration was significantly longer with protein-fed males compared with protein-deprived males. These results are discussed in the context of methoprene and/or dietary protein as prerelease treatment of sterile males in area-wide control of melon fly integrating the sterile insect technique （SIT）.

关键词： Bactrocera cucurbitae methoprene protein rich diet remating behavior SIT Tephritidae

The chemosensory appendage proteome of Amblyomma americanum （Acari： Ixodidae） reveals putative odorant-binding and other chemoreception-related proteins

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Insect Science 2017年第5期24卷 730-742页

作者： Robert Renthal Leena Manghnani Sandra Bernal Yanyan Qu Wendell P. Griffith Kim Lohmeyer Felix O. Guerrero Ligia M.F. Borges Adalberto Perez de Leon Department of Biology University of Texas at San Antonio San Antonio TX 78249 USA Department of Biochemistry University of Texas Health Science Center San Antonio IX 78229 USA RCMI Protein Biomarkers Core University of Texas at San AntonioSan Antonio TX 78249 USA Department of Chemistry University of Texas at San Antonio San Antonio TX 78249 USA usda-ars Knipling-Bushland U.S. Livestock Insects Research Laboratory Veterinary Pest Genomics Center Kerrville TX 78029 USA Instituto de Patologia Tropical e Saude Publica Universidade Federal de Goias Goiania Goias Brazil

Proteomic analyses were done on 2 chemosensory appendages of the lone star tick, Amblyomma americanum. Proteins in the fore tarsi, which contain the olfactory Haller＇s organ, and in the palps, that include gustatory sensilla, were compared with proteins in the third tarsi. Also, male and female ticks were compared. Proteins were identified by sequence similarity to known proteins, and by 3-dimensional homology modeling. Proteomic data were also compared with organ-specific transcriptomes from the tick Rhipicephalus microplus. The fore tarsi express a lipocalin not found in the third tarsi or palps. The fore tarsi and palps abundantly express 2 proteins, which are similar to insect odorant-binding proteins （OBPs）. Compared with insect OBPs, the tick OBP-like sequences lacked the cysteine absent in C-minus OBPs, and 1 tick OBP-like sequence had additional cysteines that were similar to C-plus OBPs. Four proteins similar to the antibiotic protein microplusin were found： 2 exclusively expressed in the fore tarsi and 1 exclusively expressed in the palps. These proteins lack the microplusin copper-binding site, but they are modeled to have a significant internal cavity, potentially a ligand-binding site. Proteins similar to the dust mite allergens Der p7 and Der f 7 were found differentially expressed in female fore tarsi. A protein exclusively expressed in the fore tarsi has similarities to Neto, which is known to be involved in clustering ofionotropic glutamate receptors. These results constitute the first report of OBP-like protein sequences in ticks and point to several research avenues on tick chemosensory reception.

关键词： dust mite allergen Haller＇s organ lipocalin microplusin odorant-binding protein 3-dimensional homology modeling

The role of latitudinal, genetic and temperature variation in the induction of diapause of Papilio glaucus （Lepidoptera： Papilionidae）

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Insect Science 2018年第2期25卷 328-336页

作者： Ryan, Sean F. Valella, Patti Thivierge, Gabrielle Aardema, Matthew L. Scriber, J. Mark Department of Biological Sciences University of Notre Dame Notre Dame Indiana USA usda‐ars center for Medical Agricultural and Veterinary Entomology 1600/1700 Southwest 23rd Drive Gainesville Florida USA Department of Entomology Michigan State University East Lansing Michigan USA Life Science Department Long Beach City College Long Beach California USA Sackler Institute for Comparative genomics American Museum of Natural History New York New York USA McGuire center for Lepidoptera and Diversity University of Florida Gainesville Florida USA

A key adaptation in insects for dealing with variable environmental conditions is the ability to diapause. The tiger swallowtail butterflies, Papilio glaucus and P. canadensis are ideal species to explore the genetic causes and population genetic consequences of diapause because divergence in this trait is believed to be a salient factor in maintaining a hybrid zone between these species. Yet little is known about the factors that influence diapause induction in this system. Here we explored how spatial （latitudinal）, environmental （temperature） and genetic （hybridization） factors affect diapause induction in this system. Specifically, a series of growth chamber experiments using wild caught individuals from across the eastern United States were performed to：（1） evaluate how critical photoperiod varies with latitude, （2） isolate the stage in which induction occurs, （3） test whether changes in temperature affected rates of diapause induction, and （4） explore how the incidence of diapause is affected in hybrid offspring. We find that induction occurs in the larval stage, is not sensitive to a relatively broad range of temperatures, appears to have a complex genetic basis （i.e., is not simply a dominant trait following a Mendelian inheritance pattern） and that the critical photoperiod increases by 0.4 h with each increasing degree in latitude. This work deepens our understanding of how spatial, environmental and genetic variation influences a key seasonal adaptation （diapause induction） in a well-developed ecological model system and will make possible future studies that explore how climatic variation affects the population dynamics and genetics of this system.

关键词： adaptation critical photoperiod development diapauses facultative hybridization

Performance index: An expeditious tool to screen for improved drought resistance in the Lathyrus genus

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Journal of Integrative Plant Biology 2014年第7期56卷 610-621页

作者： Susana Silvestre Susana de Sousa Araújo Maria Carlota Vaz Patto Jorge Marques da Silva center for Biodiversity Functional and Integrative Genomics(BioFIG)and Department of Plant BiologyFaculty of ScienceUniversity of LisbonCampo Grande Animal and veterinary Sciences Research center Tropical Research Institute(IICT‐CVZ)Faculty of Veterinary Medicine Plant Cell Biotechnology Lab Institute for Chemistry and Biological Technology António Xavier-New University of Lisbon(ITQB‐UNL)

Some species of the Lathyrus genus are among the most promising crops for marginal lands, with high resilience to drought, flood, and fungal diseases, combined with high yields and seed nutritional value. However, lack of knowledge on the mechanisms underlying its outstanding performance and methodologies to identify elite genotypes has hampered its proper use in breeding. Chlorophyl a fast fluorescence transient （JIP test）, was used to evaluate water deficit （WD） resistance in Lathyrus genus. Our results reveal unaltered photochemical values for al studied genotypes showing resistance to mild WD. Under severe WD, two Lathyrus sativus genotypes showed remarkable resilience maintaining the photochemical efficiency, contrary to other genotypes studied. Performance index （PIABS） is the best parameter to screen genotypes with improved performance and grain production under WD. Moreover, we found that JIP indices are good indicators of genotypic grain production under WD. Quantum yield of electron transport （wEo） and efficiency with which＆trapped excitons can move electrons further than QA （c0） revealed as important traits related to improved photosyn-thetic performance and should be exploited in future Lathyrus germplasm improvements. The JIP test herein described showed to be an expeditious tool to screen and to identify elite genotypes with improved drought resistance.

关键词： Grain production JIP test Lathyrus germplasm photochemical performance water deficit

Relevance of Advanced Plant Disease Detection Techniques in Disease and pest Management for Ensuring Food Security and Their Implication: A Review

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American Journal of Plant Sciences 2023年第11期14卷 1260-1295页

作者： Matthew Abu John Ibukunoluwa Bankole Oluwatayo Ajayi-Moses Tofunmi Ijila Timilehin Jeje Patil Lalit Department of Plant Pathology North Dakota State University Fargo ND USA Department of genomics Phenomics and Bioinformatics North Dakota State University Fargo ND USA usda-ars Cereal Crops Research Unit Edward T. Schafer Agricultural Research Center Fargo ND USA Department of Agriculture Agribusiness and Environmental Sciences Texas A&M University Kingsville TX USA Department of Botany and Plant Pathology Purdue University West Lafayette IN USA Department of Plant Pathology Indian Agricultural Research Institute New Delhi India

Plant diseases and pests present significant challenges to global food security, leading to substantial losses in agricultural productivity and threatening environmental sustainability. As the world’s population grows, ensuring food availability becomes increasingly urgent. This review explores the significance of advanced plant disease detection techniques in disease and pest management for enhancing food security. Traditional plant disease detection methods often rely on visual inspection and are time-consuming and subjective. This leads to delayed interventions and ineffective control measures. However, recent advancements in remote sensing, imaging technologies, and molecular diagnostics offer powerful tools for early and precise disease detection. Big data analytics and machine learning play pivotal roles in analyzing vast and complex datasets, thus accurately identifying plant diseases and predicting disease occurrence and severity. We explore how prompt interventions employing advanced techniques enable more efficient disease control and concurrently minimize the environmental impact of conventional disease and pest management practices. Furthermore, we analyze and make future recommendations to improve the precision and sensitivity of current advanced detection techniques. We propose incorporating eco-evolutionary theories into research to enhance the understanding of pathogen spread in future climates and mitigate the risk of disease outbreaks. We highlight the need for a science-policy interface that works closely with scientists, policymakers, and relevant intergovernmental organizations to ensure coordination and collaboration among them, ultimately developing effective disease monitoring and management strategies needed for securing sustainable food production and environmental well-being.

关键词： Disease Management Detection Techniques Advanced Detection Sustainability Science-Policy Food Security

Tung Tree(Vernicia fordii) Genome Provides A Resource for Understanding Genome Evolution and Improved Oil Production

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genomics, Proteomics & Bioinformatics 2019年第6期17卷 558-575页

作者： Lin Zhang Meilan Liu Hongxu Long Wei Dong Asher Pasha Eddi Esteban Wenying Li Xiaoming Yang Ze Li Aixia Song Duo Ran Guang Zhao Yanling Zeng Hao Chen Ming Zou Jingjing Li Fan Liang Meili Xie Jiang Hu Depeng Wang Heping Cao Nicholas J.Provart Liangsheng Zhang Xiaofeng Tan Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees Ministry of EducationCentral South University of Forestry and TechnologyChangsha 410004China Key Lab of Non-wood Forest Products of State Forestry Administration College of ForestryCentral South University of Forestry and TechnologyChangsha 410004China Department of Cell and Systems Biology/Centre for the Analysis of Genome Evolution and Function University of TorontoTorontoOntario M5S 3B2Canada State Key Laboratory of Ecological pest Control for Fujian and Taiwan Crops Fujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyFujian Agriculture and Forestry UniversityFuzhou 350002China College of Forestry Nanjing Forestry UniversityNanjing 210037China Nextomics Biosciences Co. Wuhan 430073China Oil Crop Research Institute Chinese Academy of Agricultural SciencesWuhan 430062China US Department of Agriculture Agricultural Research ServiceSouthern Regional Research CenterNew OrleansLA 70124USA

Tung tree(Vernicia fordii) is an economically important woody oil plant that produces tung oil rich in eleostearic acid. Here, we report a high-quality chromosome-scale genome sequence of tung tree. The genome sequence was assembled by combining Illumina short reads, Pacific Biosciences single-molecule real-time long reads, and Hi-C sequencing data. The size of tung tree genome is 1.12 Gb, with 28,422 predicted genes and over 73% repeat sequences. The V. fordii underwent an ancient genome triplication event shared by core eudicots but no further wholegenome duplication in the subsequent ca. 34.55 million years of evolutionary history of the tung tree lineage. Insertion time analysis revealed that repeat-driven genome expansion might have arisen as a result of long-standing long terminal repeat retrotransposon bursts and lack of efficient DNA deletion mechanisms. The genome harbors 88 resistance genes encoding nucleotide-binding sites;17 of these genes may be involved in early-infection stage of Fusarium wilt resistance. Further, 651 oil-related genes were identified, 88 of which are predicted to be directly involved in tung oil biosynthesis. Relatively few phosphoenolpyruvate carboxykinase genes, and synergistic effects between transcription factors and oil biosynthesis-related genes might contribute to the high oil content of tung seed. The tung tree genome constitutes a valuable resource for understanding genome evolution, as well as for molecular breeding and genetic improvements for oil production.

关键词： Tung tree genome Tung oil Genome evolution Electronic fluorescent pictographic browser Oil biosynthesis

Mobilizing Crop Biodiversity

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Molecular Plant 2020年第10期13卷 1341-1344页

作者： Susan McCouch Zahra Katy Navabi Michael Abberton Noelle L.Anglin Rosa Lia Barbieri Michael Baum Kirstin Bett Helen Booker Gerald L.Brown Glenn J.Bryan Luigi Cattivelli David Charest Kellye Eversole Marcelo Freitas Kioumars Ghamkhar Dario Grattipaglia Robert Henry Maria Cleria Valadares Inglis Tofazzal Islam Zakaria Kehel Paul J.Kersey Graham J.King Stephen Kresovich Emily Marden Sean Mayes Marie Noelle Ndjiondjiop Henry T.Nguyen Samuel Rezende Paiva Roberto Papa Peter W.B.Phillips Awais Rasheed Christopher Richards Mathieu Flouard Maria Jose Amstalden Sampaio Uwe Scholz Paul D.Shaw Brad Sherman S.Evan Staton Nils Stein Jan Svensson Mark Tester Jose Francisco Montenegro Valls Rajeev Varshney Stephen Visscher Eric von Wettberg Robbie Waugh Peter Wenzl Loren H.Rieseberg Plant Breeding and Genetics School of Integrated Plant SciencesCornell UniversityIthacaNY14853USA DivSeek Global Institute for Food Security110 Gymnasium PlaceUniversity of SaskatchewanSaskatoonSKS7N 0W9Canada lnternational Institute of Tropical Agriculture(IITA) PMB 5320Oyo RdIbadanNigeria International Potato center(CIP)1895 Avenida La Molina Lima Peru 12Lima 15023Peru Embrapa Genetic Resources and Biotechnology Parque Estacjao BiologicaFinal Av W5 NorteCaixa Postal 0237270770-917-Brasilia DFBrazil International center for Agricultural Research in the Dry Areas(I CAR DA) Station Exp.INRA-Quich.Rue Hafiane Cherkaoui.Agdal.Rabat-Instituts10111RabatMorocco Department of Plant Sciences University of Saskatchewan51 Campus Dr.SaskatoonSK S7N 5A8Canada Department of Plant Agriculture University of GuelphRm 316Crop Science Bldg50 Stone Rd EGuelphON N1G 2W1Canada Genome Prairie 111 Research DriveSuite 101SaskatoonSKS7N 3R2Canada The James Hutt on Institute Errol RoadInvergowrieDundeeDD25DAUK CREA Research Centre for Genomics and Bioinformaticsvia San Protaso 302Fiorenzuola d'Arda29017Italy Genome British Columbia 400-575 West 8th AvenueVancouverBCV5Z 0C4Canada International Wheat Genome Sequencing Consortium 2841 NE Marywood CtLee's SummitMO.64086USA Forage Science Grasslands Research CentreAgResearchPalmerston North4410New Zealand Queensla nd Alliance for Agriculture and Food Inn ovation University of QueenslandBrisbaneQLD 4072Australia Institute of Biotechnology and Genetic Engineering(IBGE) Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur 1706Bangladesh Southern Cross University PO Box 157LismoreNSW 2480Australia Royal Botanic Gardens KewRichmondSurreyTW93AEUK Feed the Future Innovation Lab for Crop Improvement 431 Weill HallCornell UniversityIthacaNY14853USA Department of Botany and Biodiversity Research Centre University of British ColumbiaVancouverBC V6R 2A5Canada Crops For the Future(UK)CIC 76-80 Baddow Road ChelmsfordEs

Over the past 70 years,the world has witnessed extraordinary growth in crop productivity,enabled by a suite of technological advances,including higher yielding crop varieties,improved farm management,synthetic agrochemicals,and agricultural mechanization.While this"Green Revolution"intensified crop production,and is credited with reducing famine and malnutrition,its benefits were accompanied by several undesirable collateral effects(Pingali,2012).These include a narrowing of agricultural biodiversity,stemming from increased monoculture and greater reliance on a smaller number of crops and crop varieties for the majority of our calories.This reduction in diversity has created vulnerabilities to pest and disease epidemics,climate variation,and ultimately to human health(Harlan,1972).

关键词： intensified crops agricultural