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Horticulture research 2021年第1期8卷 1363-1373页

作者： Ramesh K.Ramasamy Ming-Cheng Luo Charles A.Leslie Dianne Velasco Natalia Ott Ali McClean Abhaya M.Dandekar Mallikarjuna Aradhya Patrick J.Brown Gregory T.Browne Daniel A.Kluepfel Andreas Westphal Jan Dvorak Department of Plant Sciences University of CaliforniaDavisUSA usda-ars crops Pathology and Genetics research Unit Department of Plant PathologyUniversity of CaliforniaDavisUSA National Clonal Germplasm Repository USDA-ARSUniversity of CaliforniaDavisUSA Department of Nematology University of CaliforniaRiversideUSA

Soil-borne plant pathogens represent a serious threat that undermines commercial walnut(Juglans regia)production *** gall,caused by Agrobacterium tumefaciens,and Phytophthora root and crown rots,caused by various Phytophthora spp.,are among the most devastating walnut soil-borne diseases.A recognized strategy to combat soil-borne diseases is adoption of resistant ***,resistance to ***,***,and *** is mapped in the genome of Juglans microcarpa,a North American wild relative of cultivated ***-sib *** mother trees DJUG 31.01 and DJUG 31.09 were crossed with *** ***,producing 353 and 400 hybrids,*** propagated hybrids were genotyped by sequencing to construct genetic maps for the two populations and challenged with the three *** to each of the three pathogens was mapped as a major QTL on the long arm of *** chromosome 4D and was associated with the same haplotype,designated as haplotype b,raising the possibility that the two mother trees were heterozygous for a single Mendelian gene conferring resistance to all three *** deployment of this haplotype in rootstock breeding will facilitate breeding of a walnut rootstock resistant to both crown gall and Phytophthora root and crown rots.

关键词： breeding regia soil

Identification and Characterization of microRNA during Bemisia tabaci Infestations in Solanum lycopersicum and Solanum habrochaites

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horticultural Plant Journal 2018年第2期4卷 62-72页

作者： WANG Ketao SU Xiaomei CUI Xia DU Yongchen ZHANG Shuaibin GAO Jianchang Key laboratory of Biology and Genetic Improvement of horticultural crops of the Ministry of Agriculture Sino-Dutch Joint Laboratory of Horticultural GenomicsInstitute of Vegetables and FlowersChinese Academy of Agricultural Sciences College of Horticulture China Agricultural University

MicroRNAs(miRNAs) are a class of small non-coding RNAs that can regulate target gene expression during many plant growth and development processes. In recent years, several studies identified the miRNAs involved in fruit development, leaf development, and abiotic responses in tomato. However, little is known about the miRNAs that respond to insect attack. Here, miRNAs were identified by high-throughput sequencing at different stages after infections by the whitefly Bemisia tabaci in Solanum lycopersicum and Solanum habrochaites, which are susceptible and resistant to whitefly, respectively. A total of 44 known miRNA families were identified, and 33 were shared between the two species. Among these, 13 miRNA families were newly reported in tomato. After strict filtering, some novel miRNAs were also discovered. The global expression patterns of the miRNAs exhibited were different between the two species, reflecting their different responses and resistance levels to whitefly attack. Some of the predicted target genes of differentially expressed miRNAs may be involved in responding to, and defending against, diseases and insects. Thus, plant miRNAs are important in the responses to, and resistance against, insects and provide a useful resource for further investigations into the mechanism of miRNA-mediated plant–insect interactions.

关键词： Solanum lycopersicum Solanum habrochaites Bemisia tabaci miRNAs whitefly infection high-throughput sequencing

The technical challenges associated with commercialism of non-engineered Baculoviruses

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An optimized protocol using Steedman’s wax for high-sensitivity RNA in situ hybridization in shoot apical meristems and flower buds of cucumber

International Conference:Technology Transfer in Biological Control:From research to Practice

作者： P.V.VAIL usda ARSHorticultural Crops Research Laboratory2021 S.Peach AvenueFresnoCA 93727USA

＜正＞Interest in the development of baculoviruses as microbial control agents has increased *** the last five years many small and large organizations have been conducting basic and applied research to develop a broad spectrum of baculoviruses into commercial entities.A number of baculoviruses have been registered in Europe and the United States as a

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Journal of Integrative Agriculture 2023年第2期22卷 464-470页

作者： WANG Cui SUN Jin-jing YANG Xue-yong WAN Li ZHANG Zhong-hua ZHANG Hui-min College of Horticulture Qingdao Agricultural University/Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province/Laboratory of Quality&Safety Risk Assessment for Fruit(Qingdao)Ministry of Agriculture and Rural AffairsQingdao 266109P.R.China Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural Affairs/Sino-Dutch Joint Laboratory of Horticultural GenomicsBeijing 100081P.R.China

In situ mRNA hybridization(ISH)is a powerful tool for examining the spatiotemporal expression of genes in shoot apical meristems and flower buds of *** most common ISH protocol uses paraffin wax;however,embedding tissue in paraffin wax can take a long time and might result in RNA degradation and decreased ***,we developed an optimized protocol to simplify the process and improve RNA *** combined embedding tissue in low melting-point Steedman’s wax with processing tissue sections in solution,as in the whole-mount ISH method in the optimized *** the optimized protocol,we examined the expression patterns of the CLAVATA3(CLV3)and WUSCHEL(WUS)genes in shoot apical meristems and floral meristems of Cucumis sativus(cucumber)and Arabidopsis thaliana(Arabidopsis).The optimized protocol saved 4–5 days of experimental period compared with the standard ISH protocol using paraffin ***,the optimized protocol achieved high signal *** optimized protocol was successful for both cucumber and Arabidopsis,which indicates it might have general applicability to most plants.

关键词： cucumber in situ hybridization Steedman’s wax paraffin wax

Dwarfi ng apple rootstock responses to elevated temperatures: A study on plant physiological features and transcription level of related genes

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Journal of Integrative Agriculture 2016年第5期15卷 1025-1033页

作者： ZHOU Bei-bei SUN Jian LIU Song-zhong JIN Wan-mei ZHANG Qiang WEI Qin-ping Institute of Forestry & Pomology Beijing Academy of Agriculture & Forestry Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China) Ministry of Agriculture

The aim of this study was to investigate the impact of heat stress on physiological features, together with endogenous hormones and the transcription level of related genes, to estimate the heat resistance ability and stress injury mechanism of different dwarfing apple rootstocks. Among the six rootstocks, the rootstocks of native Shao series（SH series） showed better heat stress resistance than those of Budagovski 9（B9）, Cornell-Geneva 24（CG24）, and Malling 26（M26） from abroad. Among SH series rootstocks, SH1 and SH6 showed higher heat stress resistance than SH40. M26 demonstrated the lowest adaption ability to heat stress, showing higher leaf conductivity and lower liquid water content（LWC） with the increase in temperature. Heat stress also resulted in the suppression of photosynthesis, which showed no significant restoration after 7-day recovery. It should be noted that although a higher temperature led to a lower LWC and photosynthetic efficiency（P_n） of CG24, there was no significant increase in leaf conductivity, and 7 days after the treatment, the P_n of CG24 recovered. The extremely high temperature tolerance of SH series rootstocks could be related to the greater osmotic adjustment（OA）, which was reflected by smaller reductions in leaf relative water content（RWC） and higher turgor potentials and leaf gas exchange compared with the other rootstocks. Determination of hormones indicated multivariate regulation, and it is presumed that a relatively stable expression levels of functional genes under high-temperature stress is necessary for heat stress resistance of rootstocks.

关键词： dwarfing apple rootstock SH series rootstocks heat stress physiological features

Genetic diversity and population structure analysis of 161 broccoli cultivars based on SNP markers

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horticultural Plant Journal 2021年第5期7卷 423-433页

作者： Jingjing Huang Yumei Liu Fengqing Han Zhiyuan Fang Limei Yang Mu Zhuang Yangyong Zhang Honghao Lv Yong Wang Jialei Ji Zhansheng Li Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of AgricultureBeijing 100081China

To better understand the genetic diversity and population structure of broccoli cultivars planted in China,a total of 161 representative broccoli cultivars in the past 25 years were collected and analysed based on single nucleotide polymorphism(SNP)*** pairs of primers with good polymorphism and high resolution were screened from 315 pairs of SNP primers by 3 broccoli accessions(inbred lines)with different phenotypes and *** 10 pairs of SNP primers were selected,producing 78 *** diversity analysis indicated that the polymorphism information content(PIC)of SNP primer ranged from 0.64 to *** observed number of alleles(Na)was 2.00,the effective number of alleles(Ne)was 1.11–2.00,the Nei’s gene diversity(H)was 0.10–0.50,and Shannon information index(I)was 0.20–0.70 using PopGene32 *** clustering results showed that the 161 broccoli cultivars could be divided into 4 major subgroups(A,B,C and D),foreign cultivars were all assigned to subgroup A,and domestic cultivars were assigned to 3 subgroups of B,C,and *** study indicated that some domestic cultivars and foreign cultivars were similar in genetic background,but most domestic cultivars were still different from the Japanese *** K=2,the population structure result presented that 161 broccoli cultivars could be divided into 1 simple group(2 groups)and 1 mixed *** Q≥0.6,143(88.82%)broccoli cultivars belonged to the simple *** simple groups 68(42.24%)broccoli cultivars of group 1 were derived from Japan,the United States,Switzerland,the Netherlands,China-Taiwan,and China-Mainland;75(46.58%)broccoli cultivars belonged to group 2;when Qars belonged to the mixed *** study is helpful to understand the diversity and resolution of broccoli cultivars from worldwide,which is beneficial to plant breeding and materials *** meanwhile,this result is also used for construction of broccoli fingerprint serving for cultivar id

关键词： Broccoli Genetic diversity Population structure analysis Fingerprint SNP

Formulation and application strategies for the biocontrol of weeds in crops

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Formulation and application strategies for the biocontrol of...

Quantification and analysis of anthocyanin and flavonoids compositions,and antioxidant activities in onions with three different colors

International Conference:Technology Transfer in Biological Control:From research to Practice

作者： W.J.CONNICK D.J.DAIGLE C.D.BOYETTE P.C.QUIMBY usda ARSSouth Regional Research Center usda ARSSWSL usda ARSRWLCulbertson HallMontana State University

Formulation and application technology must address shortcomings in the efficacy and shelf life of *** emulsion and vegetable oil emulsion carriers were developed that reduced fungal weed pathogens’ (mycoherbicide ag...

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Journal of Integrative Agriculture 2016年第9期15卷 2175-2181页

作者： ZHANG Shi-lin DENG Peng XU Yu-chao Lü Shan-wu WANG Jian-jun State Key laboratory of Crop Genetics and Germplasm Enhancement Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China Ministry of Agriculture/College of Horticulture Nanjing Agricultural University

Onion（Allium cepa L.） is a very important vegetable crop widely cultivated throughout the world. In this study, we measured total anthocyanins and flavonols of onions of three colors. The fresh weight（FW） of total anthocyanins were（29.99±1.19）,（9.64±1.30） and（0.75±0.40） mg 100 g^–1 fruit in red, yellow and white onions, respectively. Likewise, the FW of total flavonoids were（111.10±5.98）,（36.64±3.59）, and 0 mg 100 g^–1 in red, yellow and white, respectively. Four types of anthocyanins（delphinidin 3,5-diglycosides, cyanidin 3,5-diglycosides, cyanidin 3-glycosides and cyanidin 3-（6′′-malonyl）-glucopyranoside） and two kinds of flavonoids（quercetin and quercetin 3-glycosides） were identified in two varieties（red and yellow bulb） of onions by HPLC/DAD-ESI/MS. The total polyphenol contents were also measured by means of Folin-Ciocalteu method. Moreover, all the concentrations of anthocyanins, flavonoids and polyphenols showed significantly positive correlations with antioxidant activities measured by DPPH˙, ABTS˙＋ and FRAP assays. This study provided information on anthocyanin and flavonoids compositions that will be useful for onion breeding.

关键词： onion(Allium cepa L.) anthocyanins flavonoids antioxidant activity HPLC-DAD-ESI/MS

Identification and Characterization of a LEA-like Gene, CaMF5,Specifically Expressed in the Anthers of Male-fertile Capsicum annuum

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horticultural Plant Journal 2020年第1期6卷 39-48页

作者： Yudan Wang Guoju Chen Jianjun Lei Bihao Cao Changming Chen Key laboratory of Biology and Germplasm Enhancement of horticultural crops in South China Ministry of AgricultureCollege of HorticultureSouth China Agricultural University

To improve the understanding of molecular mechanisms of anther and/or pollen development in Chili pepper, in the present study, fulllength cDNA and DNA sequences of the pollen development-related gene CaMF5 were obtained from the anthers of a Capsicum annuum nuclear male-fertile line. Sequence analysis indicated that the full length of CaMF5 was 747 bp, containing a maximum opening reading frame of 447 *** acid sequence alignment and phylogenetic analysis revealed that CaMF5 shared approximately 37%–77% homology with a series of uncharacterized or hypothetical proteins and late embryogenesis abundant(LEA) proteins from other plants. However, no LEA structural domain was detected in CaMF5, which indicated that it might be a new type of LEA gene. CaMF5 was only expressed in flower buds at stages 7 and 8 and in open flowers of the male-fertile line, whereas it exhibited no expression in any examined organs of the male-sterile line. In addition, CaMF5 showed the highest transcript abundance in the anthers of the male-fertile line, with no expression being detected in any other examined organs, such as the sepals, petals, pistils, roots, stems, or leaves. Taken together, our results suggest that CaMF5 is an anther-specific gene that might encode a new type of LEA protein related to anther and/or pollen development in C. annuum.

关键词： Capsicum annuum CaMF5 Anther-specific gene Late embryogenesis abundant Expression analysis

Dissection of two QTL clusters underlying yield-related heterosis in the cabbage founder parent 01–20

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horticultural Plant Journal 2023年第1期9卷 77-88页

作者： Xing Li Honghao Lv Bin Zhang Zhiyuan Fang Limei Yang Mu Zhuang Yumei Liu Zhansheng Li Yong Wang Yangyong Zhang Key laboratory of Biology and Genetic Improvement of horticultural crops Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesMinistry of Agriculture and Rural AffairsBeijingChina

Cabbage has significant heterosis and most commercial cultivars are *** explore genetic basis of cabbage heterosis and promote cabbage heterosis utilization,we constructed two populations by crossing 100 DH lines derived from a cabbage hybrid 01–20×96–100 with two female *** exhibited different extents of heterosis,the mean value of economic yield was 2.6 times bigger than *** identified 66 and 73 QTLs associated with mid-parent heterosis and transgressive heterosis of twelve yield-related traits,*** QTLs could be detected under the two-year experiment existed in two populations with different testers,showing relatively high phenotypic contribution rate(15.8%–20.0%).Heterosis QTLs exhibited clustered distribution in several cabbage chromosome *** dominant genetic regions,mk300–316 and mk258–268,originated from the elite parent 01–20,exhibited significant genetic effects for yield-related heterosis,which were first *** elite DH lines(D22,D46,D83)harboring these two dominant regions were selected as having strong heterosis in cabbage *** gene analysis revealed that some genes participating in biosynthetic processes of carbohydrates and some responses to auxin might affect cabbage yield *** identification and genetic dissection of yield-related traits provide new insights into the genetic effects of cabbage heterosis.

关键词： Cabbage Yield-related traits Heterosis QTL Genetic analysis New inbred line breeding