Genetic diversity and population structure analysis of 161 broccoli cultivars based on SNP markers

作     者：Jingjing Huang Yumei Liu Fengqing Han Zhiyuan Fang Limei Yang Mu Zhuang Yangyong Zhang Honghao Lv Yong Wang Jialei Ji Zhansheng Li Jingjing Huang;Yumei Liu;Fengqing Han;Zhiyuan Fang;Limei Yang;Mu Zhuang;Yangyong Zhang;Honghao Lv;Yong Wang;Jialei Ji;Zhansheng Li

作者机构：Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of AgricultureBeijing 100081China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2021年第7卷第5期

页      面：423-433页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：funded by the National Key Research and Development Plan(Grant No.2017YFD0101805) the National Science and Technology Foundation(Grant No.31501761) the National Modern Agricultural Industry Technology System Construction Special Fund Project(Grant No.CARS-23-A8) the Chinese Academy of Agricultural Sciences Science and Technology Innovation Project(Grant No.CAAS-ASTIP-IVF-CAAS) the State Key Laboratory of Vegetable Germplasm Innovation 

主　　题：Broccoli Genetic diversity Population structure analysis Fingerprint SNP 

摘      要：To better understand the genetic diversity and population structure of broccoli cultivars planted in China,a total of 161 representative broccoli cultivars in the past 25 years were collected and analysed based on single nucleotide polymorphism(SNP)*** pairs of primers with good polymorphism and high resolution were screened from 315 pairs of SNP primers by 3 broccoli accessions(inbred lines)with different phenotypes and *** 10 pairs of SNP primers were selected,producing 78 *** diversity analysis indicated that the polymorphism information content(PIC)of SNP primer ranged from 0.64 to *** observed number of alleles(Na)was 2.00,the effective number of alleles(Ne)was 1.11–2.00,the Nei’s gene diversity(H)was 0.10–0.50,and Shannon information index(I)was 0.20–0.70 using PopGene32 *** clustering results showed that the 161 broccoli cultivars could be divided into 4 major subgroups(A,B,C and D),foreign cultivars were all assigned to subgroup A,and domestic cultivars were assigned to 3 subgroups of B,C,and *** study indicated that some domestic cultivars and foreign cultivars were similar in genetic background,but most domestic cultivars were still different from the Japanese *** K=2,the population structure result presented that 161 broccoli cultivars could be divided into 1 simple group(2 groups)and 1 mixed *** Q≥0.6,143(88.82%)broccoli cultivars belonged to the simple *** simple groups 68(42.24%)broccoli cultivars of group 1 were derived from Japan,the United States,Switzerland,the Netherlands,China-Taiwan,and China-Mainland;75(46.58%)broccoli cultivars belonged to group 2;when Q0.6,18(11.18%)broccoli cultivars belonged to the mixed *** study is helpful to understand the diversity and resolution of broccoli cultivars from worldwide,which is beneficial to plant breeding and materials *** meanwhile,this result is also used for construction of broccoli fingerprint serving for cultivar id