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Enhancing rice resistance to fungal pathogens by transformation with cell wall degrading enzyme genes from Trichoderma atroviride

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Journal of Zhejiang University Science 2004年第2期5卷 133-136页

作者：刘梅孙宗修朱洁徐同 HARMANGaryE LORITOMatteo CollegeofAgricultureandBiotechnology ZhejiangUniversityHangzhou310029China KeyLaboratoryforRiceBiology MinistryofAgricultureChinaNationalRiceResearchInstituteHangzhou310006China DepartmentofHorticulturalSciences CornellUniversityGenevaUSA InstituteofPlantPathology NapoliUniversityTriticiItaly

Three genes encoding for fungal cell wall degrading enzymes (CWDEs), ech42, nag70 and gluc78 from the biocontrol fungus Trichoderma atroviride were inserted into the binary vector pCAMBIA1305.2 singly and in all possible combinations and transformed to rice plants. More than 1800 independently regenerated plantlets in seven different populations (for each of the three genes and each of the four gene combinations) were obtained. The ech42 gene encoding for an endochitinase increased resistance to sheath blight caused by Rhizoctonia solani, while the exochitinase-encoding gene, nag70, had lesser effect. The expression level of endochitinase but exochitinase was correlated with disease resistance. Nevertheless, exochitinase enhanced the effect of endochitinase on disease resistance when the two genes co-expressed in transgenics. Resistance to Magnaporthe grisea was found in all kinds of regenerated plants including that with single gluc78. A few lines expressing either ech42 or nag70 gene were immune to the disease. Transgenic plants are being tested to further evaluate disease resistance at field level. This is the first report of multiple of expression of genes encoding CWDEs from Trichoderma atroviride that result in resistance to blast and sheath blight in rice.

关键词： Agrobacterium-mediated transformation Rice sheath blight Rice blast Trichoderma atroviride Chitinase Glucanase

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Rice transformation with cell wall degrading enzyme genes from Trichoderma atroviride and its effect on plant growth and resistance to fungal pathogens

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浙江大学学报(农业与生命科学版) 2004年第4期 97-97页

作者： Gary E Harman,Matteo Lorito,Sheri Woo Department of Horticultural Sciences Cornell University Geneva USA Institute of Plant Pathology Napoli University tritici Italy. Institute of Plant Pathology Napoli University tritici Italy.

Three genes encoding for fungal cell wall degrading enzymes (CWDE), ech42, nag70 and gluc78 from the biocontrol fungus Trichoderma atroviride were inserted into the binary vector pCAMBIA1305.2 singly and in all possible combinations. The coding sequences were placed downstream of the rice actin promoter and all vectors were used to transform rice plants. A total of more than 1,800 independently regenerated plantlets in seven different populations (for each of the three genes and each of the four gene combinations) were obtained. Expression in plant was obtained for all the fungal genes used singly or in combinations. The ech42 gene encoding for an endochitinase increased resistance to sheath blight caused by Rhizoctonia solani, while the exochitinase-encoding gene, nag70, had a lesser effect. The expression level of endochitinase but not of the exochitinase was correlated with disease resistance. Nevertheless, exochitinase enhanced the positive effect of endochitinase on disease resistance when two genes were co-expressed in transgenic rice. Improved resistance to Magnaporthe grisea was found in all types of regenerated plants, including those with the gluc78 gene alone, while a few lines expressing either ech42 or nag70 appeared to be immune to this pathogen. Transgenic plants expressing the gluc78 gene alone were stunted and only few of them survived, even though they showed resistance to M. grisea. However, combination with either one of the two other genes (ech42, nag70) as included in the same T-DNA region, reduced the negative effect of gluc78 on plant growth. This is the first report of single or multiple of expression of transgens encoding CWDEs that results in resistance to blast and sheath blight in rice.

关键词： Agrobacterium-mediated transformation rice sheath blight rice blast Trichoderma atroviride chitinase glucanase

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Rice transformation with cell wall degrading enzyme genes from Trichoderma atroviride and its effect on plant growth and resistance to fungal pathogens

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浙江大学学报（农业与生命科学版） 2004年第4期30卷 447-447页

作者： LiuMei SunZong-Xiu ZhuJie XuTong GaryEHarman MatteoLorito SheriW∞ CollegeofAgricultureandBiotechnology ZhejiangUniversityHangzhou310029China KeyLaboratoryforRiceBiology MinistryofAgricultureChinaNationalRiceResearchInstituteHangzhou310006China DepartmentofHorticulturalSciences CornellUniversityGenevaUSA InstituteofPlantPathology NapoliUniversityTriticiItaly

关键词：水稻真菌病原体抗性木霉属植株生长细胞壁降解酶基因转化

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