Enhancing rice resistance to fungal pathogens by transformation with cell wall degrading enzyme genes from Trichoderma atroviride

作     者：刘梅 孙宗修 朱洁 徐同 HARMANGaryE LORITOMatteo 

作者机构：CollegeofAgricultureandBiotechnologyZhejiangUniversityHangzhou310029China KeyLaboratoryforRiceBiologyMinistryofAgricultureChinaNationalRiceResearchInstituteHangzhou310006China DepartmentofHorticulturalSciencesCornellUniversityGenevaUSA InstituteofPlantPathologyNapoliUniversityTriticiItaly 

出 版 物：《Journal of Zhejiang University Science》 (浙江大学学报（自然科学英文版）)

年 卷 期：2004年第5卷第2期

页      面：133-136页

核心收录：

学科分类：0810[工学-信息与通信工程] 09[农学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0901[农学-作物学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：Project (No.3997002) supported by the National Natural Science Foundation of China 

主　　题：Agrobacterium-mediated transformation Rice sheath blight Rice blast Trichoderma atroviride Chitinase Glucanase 

摘      要：Three genes encoding for fungal cell wall degrading enzymes (CWDEs), ech42, nag70 and gluc78 from the biocontrol fungus Trichoderma atroviride were inserted into the binary vector pCAMBIA1305.2 singly and in all possible combinations and transformed to rice plants. More than 1800 independently regenerated plantlets in seven different populations (for each of the three genes and each of the four gene combinations) were obtained. The ech42 gene encoding for an endochitinase increased resistance to sheath blight caused by Rhizoctonia solani, while the exochitinase-encoding gene, nag70, had lesser effect. The expression level of endochitinase but exochitinase was correlated with disease resistance. Nevertheless, exochitinase enhanced the effect of endochitinase on disease resistance when the two genes co-expressed in transgenics. Resistance to Magnaporthe grisea was found in all kinds of regenerated plants including that with single gluc78. A few lines expressing either ech42 or nag70 gene were immune to the disease. Transgenic plants are being tested to further evaluate disease resistance at field level. This is the first report of multiple of expression of genes encoding CWDEs from Trichoderma atroviride that result in resistance to blast and sheath blight in rice.