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Zoological Systematics 2022年第3期47卷 185-187页

作者： Deyuan Hong Wenying Zhuang Min Zhu Keping Ma Xiaoquan Wang Dawei Huang Yalin Zhang Guodong Ren Wenjun Bu Wanzhi Cai Dong Ren Ding Yang Aiping Liang Fengyan Bai Runzhi Zhang Fumin Lei Shuqiang Li Hongzhi Kong Lei Cai Yucheng Dai Chaodong Zhu Qisen Yang Jun Chen Zhongli Sha Jianping Jiang Jing Che Donghui Wu Jiatang Li Qiang Wang Xinli Wei Ming Bai Xingyue Liu Xuexin Chen Gexia Qiao State key laboratory of Systematic and Evolutionary Botany Institute of BotanyChinese Academy of SciencesBeijing 100093China State key laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China key laboratory of Vertebrate Evolution and Human Origins of Chinese Academy of Sciences Institute of Vertebrate Paleontology and PaleoanthropologyChinese Academy of SciencesBeijing 100044China State key laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing 100093China College of Life Sciences Nankai UniversityTianjin 300071China College of Plant Protection Northwest A&F UniversityYanglin 712100China School of Life Sciences Hebei UniversityBaoding 071002China College of Plant Protection China Agricultural UniversityBeijing 10084China key lab of Insect Evolution and Environmental Change College of Life SciencesCapital Normal UniversityBeijing 100048China School of Life Sciences Tianjin Normal UniversityTianjin 300387China key laboratory of Zoological Systematics and Evolution Institute of ZoologyChinese Academy of SciencesBeijing 100101China School of Ecology and Nature Conservation Beijing Forestry UniversityBeijing 10083China Institute of Oceanology Chinese Academy of SciencesQingdao 266071China key laboratory of Mountain Ecological Restoration and Bioresource Utilization&Ecological Restoration and Biodiversity Conservation key laboratory of Sichuan Province Chengdu Institute of BiologyChinese Academy of SciencesChengdu 610041China State key laboratory of Genetic Resources and Evolution and Yunnan Laboratory of Molecular Biology of Domestic AnimalsKunming Institute of ZoologyChinese Academy of SciencesKunming 650223China key lab of Wetland Ecology and Environment Northeast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun 130102China key laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province Institute of Insect SciencesZhejiang UniversityHangzhou 310058

Why do taxonomists matter? The work of taxonomists is often understated if not completely misunderstood. Without taxonomists, organisms cannot be accurately identified, neither can these organisms be given universally accepted names, and reliably positioned in the phylogenetic tree of life. Thanks to the work of taxonomists over the last 269 years since Carl Linnaeus established the binomial system, we can now measure the health and wealth of our biodiversity in a refined, science-based inventory prescribed by stringent nomenclatural rules.

关键词： stated prescribed refined

How to disentangle microbially functional complexity: an insight from the network analysis of C, N, P and S cycling genes

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Science Bulletin 2019年第16期64卷 1129-1131页

作者： Bang-Xiao Zheng Yi Zhao Qing-Fang Bi Guo-Wei Zhou Hong-Jie Wang Xiu-Li Hao Kai Ding key laboratory of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China Faculty of Biological and Environmental Sciences Ecosystems and Environment Research ProgrammeUniversity of HelsinkiNiemenkatu 73FI-15140 LahtiFinland Section for Microbial Ecology and Biotechnology Department of Plant and Environmental SciencesUniversity of CopenhagenThorvaldsenvej 40DK-1871 Frederiksberg CDenmark key laboratory of Subtropical Soil Science and Plant Nutrition of Zhejiang Province College of Environmental&Resource SciencesZhejiang UniversityHangzhou 310058China Department of microbiology and Ecosystem Science Research Network'Chemistry Meets Microbiology’University of ViennaVienna A-1090Austria State key lab of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Microbial Ecophysiology Group Faculty of Biology/ChemistryUniversity of BremenD-28359 BremenGermany Institute for Environmental Genomics University of OklahomaNormanOK 73019USA State key laboratory of Agricultural microbiology Huazhong Agricultural UniversityWuhan 430070China key laboratory of Arable Land Conservation(Middle and Lower Reaches of Yangtze River) Ministry of AgricultureCollege of Resources and EnvironmentHuazhong Agricultural UniversityWuhan 430070China

A complete ecosystem is also a complex network in which multifarious species interact with each other to achieve system-level functions, such as nutrient biogeochemistry (1)Microbial community is commonly considered as the primary driving force of ecosystem nutrient mobilization and metabolism, especially carbon (C), nitrogen (N), phosphorus (P), sulfur (S) and methane coupling process (2)The rise of metagenomics and high-throughput array (e.g. PhyloChip, GeoChip, etc.

关键词： net P and S cycling genes an insight from the network analysis of C How to disentangle microbially functional complexity

Comparison of gut microbiota in autism spectrum disorders and neurotypical boys in China:A case-control study

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Synthetic and Systems Biotechnology 2021年第2期6卷 120-126页

作者： Fang Ye Xinying Gao Zhiyi Wang Shuman Cao Guangcai Liang Danni He Zhitang Lv Liming Wang Pengfei Xu Qi Zhang Department of Pediatrics China-Japan Friendship HospitalBeijingChina Institute of microbiology Chinese Academy of SciencesBeijingChina College of Life Sciences Institute of Life Science and Green DevelopmentKey Lab of Microbial Diversity Research and Application of Hebei ProvinceHebei UniversityBaodingChina Clinical Research Institute China-Japan Friendship HospitalBeijingChina

Background:Autism spectrum disorders(ASDs)are a set of complex neurobiological *** evidence has shown that the microbiota that resides in the gut can modulate brain development via the gut–brain ***,direct clinical evidence of the role of the microbiota–gut–brain axis in ASD is relatively ***:A case-control study of 71 boys with ASD and 18 neurotypical controls was conducted at China-Japan Friendship *** information and fecal samples were collected,and the gut microbiome was evaluated and compared by 16S ribosomal RNA gene sequencing and metagenomic ***:A higher abundance of operational taxonomic units(OTUs)based on fecal bacterial profiling was observed in the ASD *** different microbiome profiles were observed between the two *** the genus level,we observed a decrease in the relative abundance of Escherichia,Shigella,Veillonella,Akkermansia,Provindencia,Dialister,Bifidobacterium,Streptococcus,Ruminococcaceae UCG_002,Megasphaera,Eubacterium_coprostanol,Citrobacter,Ruminiclostridium_5,and Ruminiclostridium_6 in the ASD cohort,while Eisenbergiella,Klebsiella,Faecalibacterium,and Blautia were significantly *** bacterial strains were selected for clinical discrimination between those with ASD and the neurotypical *** highest AUC value of the model was ***:Significant differences were observed in the composition of the gut microbiome between boys with ASD and neurotypical *** findings contribute to the knowledge of the alteration of the gut microbiome in ASD patients,which opens the possibility for early identification of this disease.

关键词： Autism spectrum disorders Gut microbiome China

Ginsenoside compound-K attenuates OVX-induced osteoporosis via the suppression of RANKL-induced osteoclastogenesis and oxidative stress

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Natural Products and Bioprospecting 2023年第1期13卷 159-170页

作者： Lingli Ding Zhao Gao Siluo Wu Chen Chen Yamei Liu Min Wang Yage Zhang Ling Li Hong Zou Guoping Zhao Shengnan Qin Liangliang Xu key laboratory of Orthopaedics and Traumatology Lingnan Medical Research CenterThe First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou University of Chinese MedicineGuangzhouChina Er Sha Sports Training Center of Guangdong Province GuangzhouChina School of Basic Medical Science Guangzhou University of Chinese MedicineGuangzhouChina Engineering laboratory for Nutrition Shanghai Institute of Nutrition and HealthChinese Academy of SciencesShanghaiChina Master lab for Innovative Application of Nature Products National Center of Technology Innovation for Synthetic BiologyTianjin Institute of Industrial BiotechnologyChinese Academy of SciencesTianjinChina CAS key laboratory of Quantitative Engineering Biology Shenzhen Institute of Synthetic BiologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenChina CAS-key laboratory of Synthetic Biology CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghaiChina Bio-Med Big Data Center Shanghai Institute of Nutrition and HealthChinese Academy of SciencesShanghaiChina State key laboratory of Genetic Engineering Department of Microbiology and ImmunologySchool of Life SciencesFudan UniversityShanghaiChina Department of microbiology The Chinese University of Hong KongHong KongChina Department of Orthopaedics Guangzhou Institute of Traumatic SurgeryGuangzhou Red Cross HospitalMedical CollegeJinan UniversityGuangzhouChina

Osteoporosis(OP),a systemic and chronic bone disease,is distinguished by low bone mass and destruction of bone *** Compound-K(CK),one of the metabolites of ginsenoside Rb1,has anti-aging,anti-inflammatory,anti-cancer,and hypolipidemic *** have demonstrated CK could promote osteogenesis and fracture healing in our previous ***,the contribution of CK to osteoporosis has not been *** the present study,we investigated the effect of CK on osteoclastogenesis and ovariectomy(OVX)-induced *** results showed that CK inhibited receptor activator for nuclear factor-κB ligand(RANKL)-mediated osteoclast differentiation and reactive oxygen species(ROS)activity by inhibiting the phosphorylation of NF-κB p65 and oxidative stress in RAW264.7 *** addition,we also demonstrated that CK could inhibit bone resorption using bone marrow-derived ***,we demonstrated that CK attenuated bone loss by suppressing the activity of osteoclast and alleviating oxidative stress in *** together,these results showed CK could inhibit osteoclastogenesis and prevent OVX-induced bone loss by inhibiting NF-κB signaling pathway.

关键词： Ginsenoside CK NF-κB signaling pathway Osteoporosis Oxidative stress

Lactobacillus fermentum as a new inhibitor to control advanced glycation end-product formation during vinegar fermentation

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Food Science and Human Wellness 2022年第5期11卷 1409-1418页

作者： Qin Li Leibing Li Hanjian Zhu Fan Yang Ke Xiao Lin Zhang Menglin Zhang Yongsheng Peng Chao Wang Dongsheng Li Qian Wu Mengzhou Zhou key laboratory of Fermentation Engineering(Ministry of Education) National"111"Center for Cellular Regulation and Molecular PharmaceuticsHubei Key Laboratory of Industrial MicrobiologyCooperative Innovation Center of Industry Fermentation(Ministry of Education&Hubei Province)Hubei University of TechnologyWuhan 430068China Wuhan Product Quality Supervision and Inspection Institute Wuhan 430000China

The inhibitory activity of lactic acid bacteria(lab)toward advanced glycation end-products(AGEs)during vinegar fermentation was studied,and its relationships with the substrate consumption,antioxidant capacity,total phenolic content,total flavonoid compounds,α-glucosidase,andα-amylase activity inhibition were *** vinegar was made from rice powder flour by liquid-state fermentation(LSF).The selected lab strains were separately co-cultivated with Saccharomyces cerevisiae and Acetobacter pasteurianus 1.41 in alcoholic and acetic acid fermentation,*** 3 strains,Lactobacillus fermentum showed the strongest inhibitory effect on the formation of total fluorescent AGEs and carboxymethyl lysine(CML)/carboxyethyl lysine(CEL)in the fermentation *** corresponding mechanisms included the acceleration of substrate consumtion,improvement of antioxidant activities,and inhibition ofα-glucosidase andα-*** addition,the fluorescent AGEs and the CML/CEL were negatively correlated with the antioxidant activities,while theα-glucosidase andα-amylase activities were positively correlated with the total phenols and total ***,the variety of main flavor compounds increased,including esters,alcohols,phenols and *** results of the study support the potential use of screened lab strains to inhibit the formation of fluorescent AGEs,CML and CEL on fermented products and in the food processing industry,without associated risks to consumers.

关键词： Lactobacillus fermentum Advanced glycation end-product Vinegar fermentation Antioxidant capacity

Improvement of the activity and thermostability of l‑threonine aldolase from Pseudomonas putida via tailoring of the active sites lining the binding pocket

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Systems microbiology and Biomanufacturing 2023年第3期3卷 440-448页

作者： Lihong Li Rongzhen Zhang Wenchi Zhang Yan Xu key laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology Jiangnan UniversityWuxi 214122People’s Republic of China lab of Brewing microbiology and Applied Enzymology School of BiotechnologyJiangnan UniversityWuxi 214122People’s Republic of China Solomon H.Snyder Department of Neuroscience Johns Hopkins University School of MedicineBaltimoreMD 21205USA

l-threonine aldolases catalyze the conversion of glycine and aldehydes to synthesizeβ-hydroxy-α-amino acids with unsatisfactory enzyme ***,we expressed the l-threonine aldolase from Pseudomonas putida KT2440(l-PpTA)in Escherichia coli BL21(DE3)and improved the activity and thermostability by protein *** amino acid residues(Ser10,His89,Asp93,Arg177,and Arg321)located in the substrate-binding pocket were selected and for *** mutants(D93A,D93G,D93M,D93F,D93S,D93Q,D93Y and D93H)with increased enzyme activity were identified and their k_(cat)/K_(M)values showed about 1-7-fold higher than *** all the variants,D93H showed the highest catalytic efficiency with 2925 and 4515 s^(−1)mM^(−1)of k_(cat)/K_(M)values toward l-threonine and l-allo-threonine,*** addition,circular dichroism spectrum exhibited that the melting temperature of D93H(54.2℃)was 5℃higher than wild-type(49.2℃).Molecular dynamics simulations illustrated that the D93H variant shortens the distance between the imidazole group of H93 and the hydroxyl group of substrate,which facilitated the proton extraction and promote the enzymatic *** work affords a candidate for the synthesis ofβ-hydroxy-α-amino acids with improved catalytic efficiency and thermostability and provides structural insights into the l-TA family by protein engineering.

关键词： Threonine aldolases Pseudomonas putida Catalytic efficiency Thermostability Molecular dynamics simulations

Effects of terminal modification on the catalytic efficiency and thermostability of Brucella melitensis 7α‑hydroxysteroid dehydrogenase

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Systems microbiology and Biomanufacturing 2023年第3期3卷 469-478页

作者： Zhiyong Liu Rongzhen Zhang Wenchi Zhang Yan Xu lab of Brewing microbiology and Applied Enzymology School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of EducationJiangnan UniversityWuxi 214122People’s Republic of China Solomon H.Snyder Department of Neuroscience Johns Hopkins University School of MedicineBaltimoreMD 21205USA School of Biotechnology Jiangnan University1800 Lihu AvenueWuxi City 214122China

Brucella melitensis 7α-hydroxysteroid dehydrogenase(Bm7α-HSDH)catalyzes the oxidation of chenodeoxycholic acid to 7-oxolithocholic *** this work,we investigated the effects of terminal modification(His-tags location and terminal truncation)on its catalytic efficiency and *** with C-terminal His-tagged Bm7α-HSDH(C-Bm7α-HSDH),N-Bm7α-HSDH showed a 3.6-fold higher kcat and a 1.3-fold lower Km,resulting in a 7.0-fold higher kcat/Km value toward chenodeoxycholic *** dichroism spectroscopy indicated that the melting temperature of N-Bm7α-HSDH(46.13℃)was 3.0℃lower than that of C-Bm7α-HSDH(49.13℃).N-Bm7α-HSDH produced 7-oxolithocholic acid in the highest yield of 96.7%in 4 h,whereas the C-Bm7α-HSDH gave 96.4%in 10 ***,amino acids truncation and His-tag cleave experiments confirmed the C-terminal residues played key roles in catalytic *** dynamics simulations further indicated C-terminal His-tagged modification could deform the substrate-binding region to disrupt the enzyme–substrate interactions and catalytic ***,the N-terminal His-tag hardly affected the catalytic efficiency due to its location far from the active site of the *** study provides structural insights into the terminus modifications of hydroxysteroid dehydrogenase on steroid substrate recognition and stabilization,thus affecting its catalytic functions.

关键词： Terminal modification 7α-hydroxysteroid dehydrogenase Catalytic efficiency Thermostability Molecular dynamics simulations

A recombinant chimeric protein containing B chains of ricin and abrin is an effective vaccine candidate

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A recombinant chimeric protein containing B chains of ricin ...

Amidohydrolase and amidase from Diaphorobacter sp. LR2014-1 complementally hydrolyzes various herbicides

第十一届中国生物毒素研究及医药应用年会

作者： Junhong Wang Shan Gao Zhang Tao Lin Kang Na Xu Wensen Liu Jinglin Wang State key laboratory of Pathogen and Biosecurity Institute of Microbiology and EpidemiologyAcademy of Military Medical Sciences tianjin key lab for Biomarkers Occupational and Environmental Hazard Medical College of Chinese People’s Armed Police ForceChina Institute of Military Veterinary Academy of Military Medical Sciences Jilin Medical College

＜正＞Ricin and abrin,deadly plant toxins,with the greatest potential use as biological weapons and possible bioterrorist agents,have caused growing awareness on their vaccines and related diagnosis in recent *** are similar structures with two chains linked by a disulfide bond for ricin toxin（RT）and abrin toxin（AT）.A chain confers cellular toxicity while B chain is essential for cell *** of their easy availability,stability,and extraordinary toxicity,the use of them as bioterrorist weapons is of considerable concern. Dual subunit vaccine consisting of ricin toxin B chain（RTB）and abrin toxin B chain

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Amidohydrolase and amidase from Diaphorobacter sp. LR2014-1 ...