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Allograft rejection-related gene expression in the endothelial cells of renal transplantation recipients after cytomegalovirus infection

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第11期10卷 820-828页

作者： Yang LI Hang YAN Wu-jun XUE Pu-xun TIAN Xiao-ming DING Xiao-ming PAN Xin-shun FENG Xiao-hui TIAN He-li XIANG Jun HOU Department of Renal Transplantation Center of Nephropathy the First Affiliated Hospital School of Medicine Xi 'an Jiaotong University Xi 'an 710061 China the two authors contributed equally to this work

Objective： To explore the effects of cytomegalovirus （CMV） infection on rejection-related gene expression in the endothelial cells of renal transplantation recipients. Methods： Endothelial cells （ECs） were cultured and stimulated by a variety of factors： A, normal control group; B, inactivated human cytomegalovirus （HCMV） infection group; C, HCMV infection group; D, HCMV supematant infection group; and E, ganciclovir HCMV group. Expression of intercellular adhesion molecule-1 （ICAM-1） and major histocompability complex （MHC） class I and class II antigens was detected by flow cytometry （FCM） and immuno- histochemistry. Results： We found characteristic CMV-infected ECs in this study. There were no significant differences among groups A, B and D （P〉0.05）. Although the expression levels of ICAM-1 were not significantly different between groups C and E （P〉0.05）, the ICAM-1 expression in these two groups was significantly higher than that in group A （P〈0.05）. ICAM-1 expression was detected in groups C and E, while there was no expression in groups A, B and D. Furthermore, there was no significant difference of ICAM-1 mRNA expression between groups C and E （P〉0.05）. Human leucocyte antigen （HLA）-ABC expression was detected in all the groups, while HLA-DR expression was only detected in groups C and E. There were no significant dif- ferences of HLA-ABC and HLA-DR expression among groups A, B and D （P〉0.05）. However, the HLA-ABC and HLA-DR expression levels in groups C and D were higher than those of the remaining groups previously reported （P〈0.05）. Meanwhile, the HLA-ABC and HLA-DR expression levels in group E were lower than those of group C （P〈0.05）. Conclusion： CMV could up-regulate the expression levels of ICAM-1 and MHC antigens, which was closely related to allograft rejection.

关键词： Renal transplantation Cytomegalovirus (CMV) Rejection Intercellular adhesion molecule-1 (ICAM-1) Major histocompability complex (MHC)

Solvothermal Synthesis of α-Fe2O3 Porous Nanobelts and Their Enhanced Acetone-sensing Properties

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Chinese Journal of Structural Chemistry 2019年第12期38卷 2089-2097页

作者： CHEN Hong-Wen DING Hao MA Jin-Liang YUE Fang JIA Xiao College of Chemistry Fuzhou UniversityFuzhou 350002China These authors contributed equally to this work and should be considered co-first authors

Porous α-Fe2O3 nanobelts have been prepared via a solvothermal route and subsequent calcination. The as-prepared nanostructure was characterized by XRD, FESEM, TEM, N2 adsorption-desorption isotherms, etc. The α-Fe2O3 nanobelts presented obvious porous structures with the length of ca. 1~2μm, width of ca. 200~350 nm and thickness of ca. 30~60 nm. It was found that the assistance of inorganic additives played an important role in the shape control of α-Fe2O3 nanostructure. The gas-sensing performance of the fabricated sensor based on α-Fe2O3 nanobelts sample was also investigated, and the response towards 1000 ppm acetone can reach 24.4. In addition, the gas-sensing conductive mechanism of the sensor was also proposed.

关键词： α-Fe2O3 nanobelt solvothermal method porous nanostructure gas-sensing performance

PFA-fixed Hsp6Osp-loaded dendritic cells as a vaccine for the control of mouse experimental allergic encephalomyelitis

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Cellular & Molecular Immunology 2014年第2期11卷 169-174页

作者： Feng Liu Hui Zheng Yuanyuan Qi Xue Wang Jianjun Yang Miaomiao Han Hart Zhang Hong Jiang These authors contributed equally to this work.

We have shown that Hsp6Osp-loaded immature dendritic cells （DC/sp） can protect mice from the induction of experimental allergic encephalomyelitis （EAE） by inducing Qa-l-restricted CD8＋ T regulatory （Treg） cells. The binding half-life between Qa-1 and Hsp6Osp is particularly short and leads to an unstable Qa- l/peptide complex that significantly decreases the efficacy of this vaccination. To prevent Qa-l/Hsp6Osp complex dissociation, we utilized paraformaldehyde （PFA） fixation to stabilize the formation of the Qa-l/Hsp6Osp complex and maximize the function of DC/sp as a vaccine to control autoimmune diseases. Compared with the non-fixed DC/sp, the fixed DC/sp （FDC/sp） showed an enhanced ability to activate Qa-l-restricted Hsp6Osp-specific CD8＋T cells in vitro and prevented EAE in vivo. Importantly, the FDC/sp maintained immune activity following cryopreservation for I week or after storage for 72 h at 4 ~C. These results indicate that PFA fixation can sustain or increase the efficacy of DC/sp by improving the stability of the Qa-l/Hsp6Osp complex on the surface of the DC/sp. In addition, PFA fixation creates a time window for DC/sp storage, transport and application. Our d^tn ~u^p__~t ~ nnt^nti^l clinic^l ..~e nf FDCI^n ~ ~ vnccine fnr the nr^v~ntinn and treatment of autnimm.n~_ di_~_~__

关键词： autoimmune disease dendritic cells Hsp6Osp PFA fixation Qa-l-restricted CD8+ T cells

Three-dimensional multimodal sub-diffraction imaging with spinning-disk confocal microscopy using blinking/ fluctuating probes

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Nano Research 2015年第7期8卷 2251-2260页

作者： Xuanze Chen Zhiping zeng Hening Wang Peng Xi Department of Biomedical Engineering College of Engineering Peking University Beijing 100871 China These authors contributed equally to this work.

Three-dimensional imaging cannot be achieved easily using previously developed localization super-resolution techniques. Here, we present a three-dimensional multimodal sub-diffraction imaging technique with spinning-disk （SD） confocal microscopy called 3D-MUSIC, which not only has all the advantages of SD confocal microscopy such as fast imaging speed, high signal-to-noise ratio, and optical-sectioning capability, but also extends its spatial resolution limit along all three dimensions. Both axial and lateral resolution can be improved simul- taneously by virtue of the blinking/fluctuating nature of modified fluorescent probes, exemplified with the quantum dots. Further, super-resolution images with dual modality can be obtained through super-resolution optical fluctuation imaging （SOFI） and bleaching/blinking-assisted localization microscopy （BALM）. Therefore, fast super-resolution imaging can be achieved with SD-SOFI by capturing only 100 frames while SD-BaLM yields high-resolution imaging.

关键词： multi-modality super-resolution microscopy three-dimensional spinning-disk confocal

Testicular defense systems： immune privilege and innate immunity

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Cellular & Molecular Immunology 2014年第5期11卷 428-437页

作者： Shutao Zhao Weiwei Zhu Shepu Xue Daishu Han These authors contributed equally to the work Department of Cell Biology Chinese Academy of Medical Sciences & Peking Union Medical College Beijing China

The mammalian testis possesses a special immunological environment because of its properties of remarkable immune privilege and effective local innate immunity. Testicular immune privilege protects immunogenic germ cells from systemic immune attack, and local innate immunity is important in preventing testicular microbial infections. The breakdown of local testicular immune homeostasis may lead to orchitis, an etiological factor of male infertility. The mechanisms underlying testicular immune privilege have been investigated for a long time. Increasing evidence shows that both a local immunosuppressive milieu and systemic immune tolerance are involved in maintaining testicular immune privilege status. The mechanisms underlying testicular innate immunity are emerging based on the investigation of the pattern recognition receptor-mediated innate immune response in testicular cells. This review summarizes our current understanding of testicular defense mechanisms and identifies topics that merit further investigation.

关键词： immune privilege innate immunity testis

Gene Delivery of SOCS3 Protects Mice from Lethal Endotoxic Shock

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Cellular & Molecular Immunology 2005年第5期2卷 373-377页

作者： Min Fang Hong Dai Guang Yu Feili Gong Department of Immunology Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China These authors contributed equally to this work

Suppressor of cytokine signaling 3 （SOCS3） was reported as a feedback inhibitor of cytokine receptor signaling by inhibiting the JAK-STAT signal transduction pathway. We sought to test the anti-endotoxic septic shock effect of liposome mediated gene delivery of SOCS3 in a lethal endotoxic shock mouse model. BALB/c mice were injected intraperitoneally with 200μg pcDNA3.1-SOCS3 cationic liposomes, while pcDNA3.1-IL-10 and empty vector as positive and negative control respectively. Forty-eight hours after gene delivery, mice were challenged with 4 μg of *** 0127：B8 LPS and 18 mg D-GaIN administered i.p. 90 min later, serum TNF-α level was determined. Survival over the next 48 h was evaluated. Peritoneal macrophages from survival mice were stimulated in vitro with 1 μg/ml LPS for 18 h, and the supernatants were harvested for determination of the amount of TNF-α. We found that gene delivery of SOCS3 significantly increase the mouse survival rate from 27.8 ± 9.6% of control group to 61.1 ± 9.6% （p 〈 0.01）. In comparison with control group （218 ± 13 pg/ml） and sham delivery group （219 ± 22 pg/ml）, gene delivery of SOCS3 reduced the level of serum TNF-α （68 ± 9 pg/ml） significantly （p 〈 0.01）. Furthermore, gene delivery of SOCS3 displayed the capacity of prevention of tolerance of peritoneal macrophages to LPS. These findings suggest that gene delivery of SOCS3 mediated by liposome is a promising approach for endotoxic septic shock treatment. Cellular ＆ Molecular Immunology.

关键词： gene delivery SOCS3 endotoxic septic shock

Composition engineering to obtain efficient hybrid perovskite light-emitting diodes

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Frontiers of Optoelectronics 2020年第3期13卷 282-290页

作者： Chuanzhong YAN Kebin LIN Jianxun LU Zhanhua WEI These authors contributed equally to this work.Institute of Luminescent Materials and Information Displays College of Materials Science and EngineeringHuaqiao UniversityXiamen 361021China

Metal halide perovskites have received considerable attention in the field of electroluminescence,and the external quantum efficiency of perovsk'c lightemitting diodes has exceeded 20%.CH3NH3PbBr3 has been intensely investigated as an emitting layer in perovskite light-emitting ***,perovskite films comprising CH3NH3PbBr3 often exhibit low surface coverage and poor crystallinity,leading to high current leakage,severe nonradiative recombination,and limited device ***,we demonstrate a rationale for composition engineering to obtain high-quality perovskite *** first reduce pinholes by adding excess CH3NH3B1 to the actual CH3NH3PbBr3 films,and we then add CsBr to improve the crystalline quality and to passivate nonradiative *** a result,the(CH3NH3)1-xCSxPbBx based perovskite light-emitting diodes exhibit significantly improved external quantum and power efficiencies of 6.97%and 25.181m/W,respectively,representing an improvement in performance dozens of times greater than that of pristine CH3NH3PbBr3-based perovskite light-emitting *** study demonstrates that composition engineering is an effective strategy for enhancing the device performance of perovskite light-emitting diodes.

关键词： perovskite light-emitting diode(LED) composition engineering ion doping

Characterization of a Novel Anti-DR5 Monoclonal Antibody WD1 with the Potential to Induce Tumor Cell Apoptosis

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Cellular & Molecular Immunology 2008年第1期5卷 55-60页

作者： Jing Wang Zhou Lin Chunxia Qiao Ming Lv Ming Yu He Xiao Qingyang Wang Liyan Wang Jiannan Feng Beifen Shen Yuanfang Ma Yan Li Laboratory of Cellular and Molecular Immunology Institute of Immunology Henan University Kaifeng China Institute of Basic Medical Sciences Beijing China These authors contributed equally to this work

TNF-related apoptosis-inducing ligand （TRAIL） is a TNF family member capable of inducing apoptosis. Death receptor 5 （DR 5） is a key receptor of TRAIL and plays an important role in TRAIL-induced apoptosis. To prepare monoclonal antibodies （mAbs） against DR5, cDNA encoding soluble DR5 （sDR5） was firstly amplified by reverse transcriptase-polymerase chain reaction （RT-PCR） with specific primers, and then inserted into a prokaryotic expression vector pET-30a. The recombinant plasmid was expressed in Escherichia coli strain BL21 （DE3）, and sDR5 was purified by nickel affinity chromatography. As an antigen, sDR5 was used to immunize mice. Hybridomas secreting antibodies against sDR5 were identified. One positive done was selected to produce antibody, WD1. ELISA and immunofluorescence demonstrated that WD1 could bind recombinant sDR5 and membranebound DR5 （mDR5） on Jurkat and Molt-4 cells. ATPLite assays showed that Jurkat and Molt-4 cells were sensitive to the antibody in a dose dependent manner. The Annexin V/PI assays and Giemsa＇s staining both showed that WD1 could induce Jurkat cell apoptosis efficiently. Transient transfection of 293T cells and indirect immunofluorescence assay demonstrated that mAb （WD1） couldn＇t cross-react with DR4. Our findings indicated that the novel antibody, WD1 could act as a direct agonist, bind DR5 characteristically, and initiate efficient apoptotic signaling and tumor regression. Thus, WD1 would be a leading candidate for potential cancer therapeutics. Cellular ＆ Molecular Immunology.

关键词： TRAIL death receptor 5 apoptosis monoclonal antibody ATPLite

MEF13 Requires MORF3 and MORF8 for RNA Editing at Eight Targets in Mitochondrial mRNAs in Arabidopsis thaliana

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Molecular Plant 2015年第10期8卷 1466-1477页

作者： Franziska Glass Barbara Hartel Anja Zehrmann Daniil Verbitskiy Mizuki Takenaka Molekulare Botanik Universitat UIm 89069 UIm Germany These authors contributed equally to this article.

RNA editing sites in plant mitochondria and plastids are addressed by pentatricopeptide repeat （PPR） proteins with E or E and DYW domains, which recognize a specific nucleotide motif upstream of the edited nucleotide. In addition, some sites require MORF proteins for efficient RNA editing. Here, we assign the novel E domain-containing PPR protein, MEF13, as being required for editing at eight sites in Arabidopsis thaliana. A SNP in ecotype C24 altering the editing level at only one of the eight target sites was located by genomic mapping. An EMS mutant allele of the gene for MEF13 was identified in a SNaPshot screen of a mutated plant population. At all eight target sites of MEF13, editing levels are reduced in both morf3 and morf8 mutants, but at only one site in morfl mutants, suggesting that specific MEF13-MORF interactions are required. Yeast two-hybrid analyses detect solid connections of MEF13 with MORF1 and weak contact with MORF3 proteins. Yeast three-hybrid （Y3H） analysis shows that the presence of MORF8 enhances the connection between MEF13 and MORF3, suggesting that a MORF3-MORF8 heteromer may form stably or transiently to establish interaction with MEF13.

关键词： RNA editing plant mitochondria PPR protein MEF13 MEF-MORF interaction