PFA-fixed Hsp6Osp-loaded dendritic cells as a vaccine for the control of mouse experimental allergic encephalomyelitis

作     者：Feng Liu Hui Zheng Yuanyuan Qi Xue Wang Jianjun Yang Miaomiao Han Hart Zhang Hong Jiang 

作者机构：These authors contributed equally to this work. 

出 版 物：《Cellular & Molecular Immunology》 (中国免疫学杂志（英文版）)

年 卷 期：2014年第11卷第2期

页      面：169-174页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

主　　题：autoimmune disease dendritic cells Hsp6Osp PFA fixation Qa-l-restricted CD8+ T cells 

摘      要：We have shown that Hsp6Osp-loaded immature dendritic cells （DC/sp） can protect mice from the induction of experimental allergic encephalomyelitis （EAE） by inducing Qa-l-restricted CD8＋ T regulatory （Treg） cells. The binding half-life between Qa-1 and Hsp6Osp is particularly short and leads to an unstable Qa- l/peptide complex that significantly decreases the efficacy of this vaccination. To prevent Qa-l/Hsp6Osp complex dissociation, we utilized paraformaldehyde （PFA） fixation to stabilize the formation of the Qa-l/Hsp6Osp complex and maximize the function of DC/sp as a vaccine to control autoimmune diseases. Compared with the non-fixed DC/sp, the fixed DC/sp （FDC/sp） showed an enhanced ability to activate Qa-l-restricted Hsp6Osp-specific CD8＋T cells in vitro and prevented EAE in vivo. Importantly, the FDC/sp maintained immune activity following cryopreservation for I week or after storage for 72 h at 4 ~C. These results indicate that PFA fixation can sustain or increase the efficacy of DC/sp by improving the stability of the Qa-l/Hsp6Osp complex on the surface of the DC/sp. In addition, PFA fixation creates a time window for DC/sp storage, transport and application. Our d^tn ~u^p__~t ~ nnt^nti^l clinic^l ..~e nf FDCI^n ~ ~ vnccine fnr the nr^v~ntinn and treatment of autnimm.n~_ di_~_~__