检索结果-南通市图书馆

Rapid generation of gene-targeted EPS-derived mouse models through tetraploid complementation

Protein & cell 2019年第1期10卷 20-30页

作者： Haibo Li Chaoran Zhao Jun Xu Yaxing Xu Chunmei Cheng Yinan Liu Ting Wang Yaqin Du Liangfu Xie Jingru Zhao Yanchuang Han Xiaobao Wang Yun Bai Hongkui Deng department of cell biology School of Basic Medical SciencesPeking University Stem Cell Research CenterState Key Laboratory of Natural and Biomimetic DrugsPeking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and DifferentiationCollege of Life SciencesPeking-Tsinghua Center for Life SciencesPeking UniversityBeijing 100191China Shenzhen stem cell Engineering Laboratory Key Laboratory of Chemical GenomicsPeking University Shenzhen Graduate SchoolShenzhen 518055China Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program College of Life SciencesPeking UniversityBeijing 100871China BeiHao stem cell and Regenerative Medicine Translational Research Institute BeijingChina

One major strategy to generate genetically modified mouse models is gene targeting in mouse embryonic stem(ES)cells,which is used to produce gene-targeted mice for wide applications in ***,a major bottleneck in this approach is that the robustness of germiine transmission of gene-targeted ES cells can be significantly reduced by their genetic and epigenetic instability after long-term culturing,which impairs the efficiency and robustness of mouse model ***,we have established a new type of pluripotent cells termed extended pluripotent stem(EPS)cells,which have superior developmental potency and robust germline competence compared to conventional mouse ES *** this study,we demonstrate that mouse EPS cells well maintain developmental potency and genetic stability after long-term *** on gene targeting in mouse EPS cells,we established a new approach to directly and rapidly generate gene-targeted mouse models through tetraploid complementation,Haibo Li and Chaoran Zhao contributed equally to this *** supplementary material The online version of this article(https://***/10.1007/s13238-018-0556-1)contains supplementary material,which is available to authorized *** could be accomplished in approximately 2 ***,using this approach,we successfully constructed mouse models in which the human interleukin 3(IL3)or interleukin 6(IL6)gene was knocked into its corresponding locus in the mouse *** study demonstrates the feasibility of using mouse EPS cells to rapidly generate mouse models by gene targeting,which have great application potential in biomedical research.

关键词： tetraploid complementation EPS mouse model CRISPR/Cas9

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The concentration game: differential effects of bioactive signaling in 2D and3D culture

引用

Neural Regeneration Research 2016年第1期11卷 66-68页

作者： Laura A.Smith Callahan The Vivian L.Smith department of Neurosurgery & center for stem cell and Regenerative Medicine University of Texas Health Science Center at Houston

Traumatic injuries to the central nervous system,such as traumatic brain injury,spinal cord injury and stroke,have a high prevalence,enormous financial costs and lack clinical treatments that restore neurological function（Ma et al.,2014）These injuries trigger a series of secondary biochemical and cellular responses that ultimately lead to cellular death and themaintenance of an unsupportive extracellular matrix （ECM） for tissue regeneration （Silva et al., 2014）. Artificial ECM or scaf- folds represent a way to alter this unsupportive environment to improve the efficacy of stem cell therapies and enhance neural tissue regeneration （Figure 1）.

关键词： bioactive injuries restore biochemical neurological traumatic signaling scaffold ultimately likely

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

NK cells play a significant role in immunosurveillance at the early stage of MLL-AF9 acute myeloid leukemia via CD226/CD155 interactions

引用

Science China(Life Sciences) 2015年第12期58卷 1288-1298页

作者： WANG YaJie CHEN Chen DONG Fang MA ShiHui XU Jing GONG YueMin CHENG Hui ZHOU Yuan CHENG Tao HAO Sha State Key Laboratory of Experimental Hematology Institute of Hematology& Blood Diseases Hospital Collaborative Innovation Center for Cancer Medicine center for stem cell Medicine Chinese Academy of Medical Sciences & Peking Union Medical College department of stem cell and Regenerative Medicine Chinese Academy of Medical Sciences & Peking Union Medical College

Acute myeloid leukemia(AML) is an aggressive hematological malignancy, and the mechanism underlying immune system involvement in leukemia development is unclear. In the present study, we utilized a myeloid/lymphoid or mixed-lineage leukemia; translocated to, 3(MLLT3/MLL-AF9)-induced AML mouse model with or without exposure to irradiation. We found that the leukemia cells could survive and expand in hosts with intact immune systems, whereas leukemia progression was accelerated in mice with impaired immune systems. Moreover, the leukemia cells escaped from host immunosurveillance via editing their immunogenicity, including the up-regulation of an inhibitory antigen(i.e., CD47) and the down-regulation of active antigens(i.e., CD86, CD54, retinoic acid early transcript(RAE), histocompatibility 2, D region locus b(H2-Db) and H2-Dd). Natural killer(NK) cells were activated in the early phase of AML progression, whereas T cells were stimulated in the late phase. Furthermore, NK cell depletion showed that NK cells were necessary for the elimination of leukemia cells in our AML mouse model. Notably, CD155/CD226 primarily mediated the interaction between NK cells and leukemia cells and contributed to the antitumor effects of NK cells during the early phase of AML. Clinical data from patients with diverse hematological malignancies showed that CD155 expression was decreased in hematological malignancies. Taken together, our results demonstrate that NK cells play a pivotal role in immunosurveillance against leukemia cells during the early stage of AML primarily through the CD226/CD155 interaction; however, NK cells are not sufficient to eliminate leukemia cells.

关键词： acute myeloid leukemia natural killer cell immunosurveillance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Gene editing in T cell therapy

引用

Journal of Genetics and Genomics 2017年第9期44卷 415-422页

作者： Yongping Zhang Wei Mu Haoyi Wang State Key Laboratory of stem cell and Reproductive biology Institute of Zoology Chinese Academy of Sciences department of Hematology Aerospace Center Hospital Aerospace Clinical Medical College Peking University University of Chinese Academy of Sciences

The adoptive transfer of engineered T cells for the treatment of cancer, autoimmunity, and infectious disease is a rapidly growing field that has shown great promise. Gene editing holds tremendous potential for further improvements of T cell therapy. Here we review the applications of gene editing in various T cell therapies, focusing on antiviral strategies and cancer immunotherapies, and discuss the challenges and future prospects.

关键词： Gene editing CRISPR-Cas9 T cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Choosing proper fluorescent dyes, proteins, and imaging techniques to study mitochondrial dynamics in mammalian cells

引用

Biophysics Reports 2017年第Z2期3卷 64-72页

作者： Xingguo Liu Liang Yang Qi Long David Weaver Gy?rgy Hajnóczky Key Laboratory of Regenerative biology Guangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineSouth China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences department of Pathology Mito Care CenterAnatomy and Cell BiologyThomas Jefferson University

Mitochondrial dynamics refers to the processes maintaining mitochondrial homeostasis, including mitochondrial fission, fusion, transport, biogenesis, and mitophagy. Mitochondrial dynamics is essential for maintaining the metabolic function of mitochondria as well as their regulatory roles in cell *** this review, we summarize the recently developed imaging techniques for studying mitochondrial dynamics including: mitochondrial-targeted fluorescent proteins and dyes, live-cell imaging using photoactivation, photoswitching and cell fusion, mitochondrial transcription and replication imaging by in situ hybridization, and imaging mitochondrial dynamics by super-resolution microscopy. Moreover,we discuss examples of how to choose and combine proper fluorescent dyes and/or proteins.

关键词： Imaging Mitochondrial dynamics Photoactivation Photoswitching Super-resolution microscope

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Mid1ip1b modulates apical reorientation of non-centrosomal microtubule organizing center in epithelial cells

引用

Journal of Genetics and Genomics 2018年第8期45卷 433-442页

作者： Xin Zhou Chun Xiao Yu Li Yanna Shang Dongqin Yin Siying Li Bo Xiang Ran Lu Yi Ji Yang Wu Wentong Meng Hongyan Zhu Jin Liu Huozhen Hu Xianming Mo Hong Xu department of Pediatric Surgery and Laboratory of stem cell biology State Key Laboratory of Biotherapy West China Hospital Sichuan University and Collaborative Innovation Center for Biotherapy National Clinical Research center of Geriatrics West China Hospital Sichuan University

In most kinds of animal cells, the centrosome serves as the main microtubule organizing center （MTOC） that nucleates microtubule arrays throughout the cytoplasm to maintain cell structure, cell division and intracellular transport. Whereas in epithelial cells, non-centrosomal MTOCs are established in the apical domain for generating asymmetric microtubule fibers and cilia in epithelial cells for the organ morphogenesis during embryonic development. However, the mechanism by which MTOCs localize to the apical domain in epithelial cells remains largely unknown. Here, we show that Mid liplb has a close interaction with γ-tubulin protein, the central component of MTOC, and modulates lumen opening of the neural tube, gut, intestine, and kidney of zebrafish. Knockdown or dominant negative effect of Mid 1ip 1 b resulted in failure of lumen formation of the organs as aforementioned. Moreover, the non-centrosomal MTOCs were unable to orientate to the apical domain in Midliplb knockdown epithelial cells, and the centrosomal MTOCs were inaccurately placed in the apical domain, resulting in defective formation of asymmetric microtubules and misplacement of cilia in the apical domain. These data uncover a molecule that controls the proper localization of MTOCs in the apical domain in epithelial cells for organ morphogenesis during embryonic development.

关键词： Mid1ip1b Microtubule organizing center Non-centrosomal MTOC Microtubule Epithelial cell

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Modulating microRNAs in cancer: next-generation therapies

引用

Cancer biology & Medicine 2022年第3期19卷 289-304页

作者： Nahid Arghiani Khalid Shah center for stem cell and Translational Immunotherapy(CSTI) Harvard Medical SchoolBostonMA 02115USA department of Neurosurgery Brigham and Women’s HospitalHarvard Medical SchoolBostonMA 02115USA Harvard stem cell Institute Harvard UniversityCambridgeMA 02138USA

MicroRNAs(miRNAs)are a class of endogenously expressed non-coding regulators of the genome with an ability to mediate a variety of biological and pathological *** is growing evidence demonstrating frequent dysregulation of microRNAs in cancer cells,which is associated with tumor initiation,development,migration,invasion,resisting cell death,and drug *** have shown that modulation of these small RNAs is a novel and promising therapeutic tool in the treatment of a variety of diseases,especially cancer,due to their broad influence on multiple cellular ***,suboptimal delivery of the appropriate miRNA to the cancer sites,quick degradation by nucleases in the blood circulation,and off target effects have limited their research and clinical ***,there is a pressing need to improve the therapeutic efficacy of miRNA modulators,while at the same time reducing their *** delivery vehicles for miRNA modulators have been shown to be effective in vitro and in *** this review,we will discuss the role and importance of miRNAs in cancer and provide perspectives on currently available carriers for miRNA *** will also summarize the challenges and prospects for the clinical translation of miRNAbased therapeutic strategies.

关键词： MiRNAs dysregulation delivery systems cancer therapy clinical translation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Reversing multiple age-related pathologies by controlling the senescence-associated secretory phenotype of stem cells

引用

Neural Regeneration Research 2016年第11期11卷 1746-1747页

作者： Daisuke Hisamatsu Hayato Naka-Kaneda Laboratory for stem cell Competency RIKEN Center for Integrative Medical Sciences(IMS) department of Physiology Keio University School of Medicine

Regenerative medicine by cell transplantation is a novel therapy for treating end-stage organ failure and tissue damage. cell-based therapy based on the transplantation of neural stem/progenitor cells （NSPCs） represents an attractive strategy for the treatment of neurodegenerative diseases, but obtaining large numbers of these cells is difficult and their differentiation potential is strictly restricted in a spatiotemporally-regulated manner during central nervous system （CNS） development. Therefore, embryonic stem cells and induced pluripotent stem cells represent an attractive alternative for cell-transplantation therapy in regenerative medicine.

关键词： cell stem Reversing multiple age-related pathologies by controlling the senescence-associated secretory phenotype of stem cells MSCs SASP

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A multi-channel collagen scaffold loaded with neural stem cells for the repair of spinal cord injury

引用

Neural Regeneration Research 2021年第11期16卷 2284-2292页

作者： Shuo Liu Yuan-Yuan Xie Liu-Di Wang Chen-Xu Tai Dong Chen Dan Mu Yan-Yan Cui Bin Wang Clinical stem cell center Nanjing Drum Tower Hospitalthe Affiliated Hospital of Nanjing University Medical SchoolNanjingJiangsu ProvinceChina department of Radiology Nanjing Drum Tower Hospitalthe Affiliated Hospital of Nanjing University Medical SchoolNanjingJiangsu ProvinceChina department of cell biology and Genetics Chongqing Medical UniversityChongqingChina

Collagen scaffolds possess a three-dimensional porous structure that provides sufficient space for cell growth and proliferation,the passage of nutrients and oxygen,and the discharge of *** this study,a porous collagen scaffold with axially-aligned luminal conduits was *** vitro biocompatibility analysis of the collagen scaffold revealed that it enhances the activity of neural stem cells and promotes cell extension,without affecting cell *** collagen scaffold loaded with neural stem cells improved the hindlimb motor function in the rat model of T8 complete transection and promoted nerve *** collagen scaffold was completely degraded in vivo within 5 weeks of implantation,exhibiting good *** temperature,C-reactive protein expression and CD68 staining demonstrated that rats with spinal cord injury that underwent implantation of the collagen scaffold had no notable inflammatory *** findings suggest that this novel collagen scaffold is a good carrier for neural stem cell transplantation,thereby enhancing spinal cord repair following *** study was approved by the Animal Ethics Committee of Nanjing Drum Tower Hospital(the Affiliated Hospital of Nanjing University Medical School),China(approval No.2019AE02005)on June 15,2019.

关键词： axially-aligned luminal conduits biomaterial cell transplantation collagen complete transection inflammation neural stem cell regeneration scaffold spinal cord injury tissue engineering

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Immunomodulation of adipose-derived mesenchymal stem cells on peripheral blood mononuclear cells in colorectal cancer patients with COVID-19

引用

World Journal of Gastrointestinal Oncology 2024年第5期16卷 2113-2122页

作者： Jun-Feng Wang Xiao-Xia Yang Jian Zhang Yan Zheng Fu-Qing Zhang Xiao-Feng Shi Yu-Liang Wang department of Colorectal Oncology Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerTianjin’s Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin 300060China department of Neurology Tianjin First Center HospitalTianjin 300192China Prosthodontics Studio Tianjin Stomatological HospitalTianjin 300041China department of Clinical Laboratory Medicine Tianjin Institute of UrologyThe Second Hospital of Tianjin Medical UniversityTianjin 300211China department of Emergency Tianjin First Central HospitalTianjin 300192China

BACKGROUND Accumulating evidence has shown that adipose tissue-derived mesenchymal stem cells(ADSCs)are an effective therapeutic approach for managing coronavirus disease 2019(COVID-19);however,further elucidation is required to determine their underlying immunomodulatory effect on the mRNA expression of T helper cell-related transcription factors(TFs)and cytokine release in peripheral blood mononuclear cells(PBMCs).AIM To investigate the impact of ADSCs on the mRNA expression of TFs and cytokine release in PBMCs from colorectal cancer(CRC)patients with severe COVID-19(CRC^(+)patients).METHODS PBMCs from CRC^(+)patients(PBMCs-C+)and age-matched CRC patients(PBMCs-C)were stimulated and cultured in the presence/absence of *** mRNA levels of T-box TF TBX21(T-bet),GATA binding protein 3(GATA-3),RAR-related orphan receptor C(RORC),and forkhead box P3(FoxP3)in the PBMCs were determined by reverse transcriptase-polymerase chain *** supernatants were evaluated for levels of interferon gamma(IFN-γ),interleukin 4(IL-4),IL-17A,and transforming growth factor beta 1(TGF-β1)using an enzyme-linked immunosorbent *** Compared with PBMCs-C,PBMCs-C+exhibited higher mRNA levels of T-bet and RORC,and increased levels of IFN-γ and ***,a significant decrease in FoxP3 mRNA and TGF-β1,as well as an increase in Tbet/GATA-3,RORC/FoxP3,IFN-γ/IL-4,and IL-17A/TGF-β1 ratios were observed in PBMCs-C+.Furthermore,ADSCs significantly induced a functional regulatory T cell(Treg)subset,as evidenced by an increase in FoxP3 mRNA and TGF-β1 release *** was accompanied by a significant decrease in the mRNA levels of T-bet and RORC,release of IFN-γ and IL-17A,and T-bet/GATA-3,RORC/FoxP3,IFN-γ/IL-4,and IL-17A/TGF-β1 ratios,compared with the PBMCs-C+*** The present in vitro studies showed that ADSCs contributed to the immunosuppressive effects on PBMCs-C+,favoring Treg ***,ADSC-based cell therapy could be a beneficial approach for p

关键词： Colorectal cancer COVID-19 Adipose-derived mesenchymal stem cells T helper cell Immunomodulation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：