检索结果-南通市图书馆

Improving Vascular Regeneration Performance of Electrospun Poly(ε‑Caprolactone) Vascular Grafts via Synergistic Functionalization with VE‑Cadherin/VEGF

引用

Advanced Fiber Materials 2022年第6期4卷 1685-1702页

作者： Yan Zhang Ke Xu Dengke Zhi Mengyuan Qian Kaizheng Liu Qizhi Shuai Zheng Qin Jinghui Xie Kai Wang Jun Yang The key laboratory of Bioactive Materials Ministry of EducationCollege of Life SciencesNankai UniversityTianjin 300071China state key laboratory of medicinal chemical biology Nankai UniversityTianjin 300350China

In vivo,vascular endothelial growth factor(VEGF)and vascular endothelial cadherin(VE-cadherin)co-regulate the dynamic organization of endothelial cells during vascular sprouting,balancing angiogenesis and vascular *** this study,a novel bioactive surface integrating human VE-cadherin-Fc and VEGF-Fc fusion proteins was innovatively developed for the modification of poly(ε-caprolactone)(PCL)small-caliber electrospun fibrous grafts(VE-cad/VEGF-PCL)to promote the regeneration of functional endothelium and improve the patency of artificial vascular *** fusion proteins self-assembled on the PCL fibers through the hydrophobic binding of Fc domains,improving surface hydrophilicity while reducing the adhesion of *** vitro results showed that the VE-cadherin/VEGF surface upregulated the expression of endogenous VE-cadherin and synergistically activated the VE-cadherin/VEGFR2/FAK/AKT/ERK signal transduction,which facilitated the functioning of human umbilical vein endothelial cells(HUVECs).Moreover,the VE-cadherin/VEGF surface significantly enhanced cellularization and capillary formation,then subsequently accelerated the regeneration of functional endothelium and smooth muscle in the VE-cad/VEGF-PCL grafts in a rat abdominal aorta replacement ***,these results highlight the advantages of VE-cadherin/VEGF surface in enhancing rapid endothelialization of electrospun vascular grafts and provide new insights into the design of cross-activating biomaterials.

关键词： VE-cadherin/VEGF Bioactive surface Biomimetic endothelium microenvironment Electrospun PCL vascular grafts Rapid endothelialization

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Precursor-involved and Conversion Rate-controlled Self-assembly of a ＇Super Gelator＇ in Thixotropic Hydrogels for Drug Delivery

引用

Chinese Journal of Chemistry 2012年第8期30卷 1781-1787页

作者：区彩文王怀民杨志谋陈敏生 Guangzhou Medical University GuangzhouGuangdong 510182China state key laboratory of medicinal chemical biology and college of life sciences Nankai UniversityTianjin 300071China

Enzymatic hydrogelation is a totally different process to the heating-cooling gelation process, in which the pre- cursors of the gelators can be involved during the formation of self-assembled structures. Using thixotropic hy- drogels formed by a super gelator as our studied system, we demonstrated that the enzyme concentration/conversion rate of enzymatic reaction had a strong influence on the morphology of resulting self-assembled nanostructures and the property of resulting hydrogels. The principle demonstrated in this study not only helps to understand and elucidate the phenomenon of self-assembly triggered by enzymes in biological systems, but also offers a unique methodology to control the morphology of self-assembled structures for specific applications such as controlled drug re- lease.

关键词： self-assembly hydrogel enzymatic reaction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

β-galactosidase responsive AIE fluorogene for identification and removal of senescent cancer cells

引用

Science China Chemistry 2020年第3期63卷 398-403页

作者： Zhengfeng Gao Heqi Gao Debin Zheng Tengyan Xu Yaoxia Chen Chunhui Liang Ling Wang Dan Ding Zhimou Yang state key laboratory of medicinal chemical biology and college of Pharmacy Nankai UniversityTianjin 300071China key laboratory of Bioactive Materials Ministry of EducationCollege of Life Sciencesand Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)Nankai UniversityTianjin 300060China

The selective identification and removal of senescent cells including senescent cancer cells are very important to prolong life and improve the treatment efficacy of cancer *** this study,we integrated the high selectivity of enzyme-instructed selfassembly(EISA)and efficient reactive oxygen species(ROS)generating property of a novel luminogen with aggregationinduced emission(AIE)character to selectively identify and remove senescent He La(s-He La)*** s-He La cells expressed high levels ofβ-galactosidase(β-Gal),which led to the selective accumulation and formation of nanomaterials of Comp.1 in the *** white light irradiation,the nanomaterials efficiently produced ROS and therefore killed s-He La *** study demonstrated a promising strategy to selectively remove senescent cells and improve the treatment efficacy of cancer therapy.

关键词： senescence cancer fluorescence self-assembly aggregation induced emission

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Supramolecular hydrogels of self-assembled zwitterionic-peptides

引用

Chinese chemical Letters 2021年第11期32卷 3636-3640页

作者： Yiming Zhang Yinghao Ding Xinxin Li Debin Zheng Jie Gao Zhimou Yang state key laboratory of medicinal chemical biology Key Laboratory of Bioactive MaterialsMinistry of Education and College of Life SciencesNankai UniversityTianjin 300071China college of life sciences Key Laboratory of Bioactive MaterialsMinistry of Educationand Collaboration Innovation Center of Chemical Science and Engineering(Tianjin)Nankai UniversityTianjin 300071China state key laboratory of medicinal chemical biology College of PharmacyTianjin Key Laboratory of Molecular Drug ResearchNankai UniversityTianjin 300071China

Zwitterionic polymer materials have been extensively studied,but zwitterionic peptides supramolecular hydrogel materials are rarely *** this study,the preparation of two zwitterionic hydrogels using self-assembled peptides were *** hydrogels could be fabricated easily by changing the temperature or enzyme catalysis in a short *** the differences in structure and function of the zwitterion peptide hydrogels caused by the two preparation methods were also be *** found that the hydrogel prepared by enzyme induced self-assembly has better solubility and lower cytotoxicity than that prepared by the heating-cooling *** result showed the enzyme induced self-assembly way to form zwitterionic peptides supramolecular hydrogel materials could have further biomedical applications.

关键词： Self-assembly Hydrogel Zwitterionic-peptides Enzyme-instructed Biocompatibility

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Endoplasmic reticulum targeted AIE bioprobe as a highly efficient inducer of immunogenic cell death

引用

Science China Chemistry 2020年第10期63卷 1428-1434页

作者： Jun Li Heqi Gao Ruihua Liu Chao Chen Sheng Zeng Qian Liu Dan Ding key laboratory of Bioactive Materials Ministry of Education College of Life Sciencesand State Key Laboratory of Medicinal Chemical BiologyNankai UniversityTianjin 300071China Department of Urology Tianjin First Central HospitalTianjin 300192China

Focused oxidative stress of the specific organelles(e.g.,endoplasmic reticulum(ER)and mitochondrion)of cancer cells can boost the immunogenic cell death(ICD)effect for cancer ***,an ER-targeted bioprobe with aggregationinduced emission(AIE)characteristics(TPE-PR-FFKDEL)was rationally designed and synthesized by integrating a new AIE photosensitizer with ER targeting peptide,which has been demonstrated to be able to efficiently induce ER oxidative stress to evoke *** with the photosensitizer hypericin that is well-known as an ER-targeted ICD inducer,TPE-PR-FFKDEL can lead to more robust emission of immunostimulatory damage-associated molecular patterns such as surface-exposed calreticulin,ATP secretion,and high-mobility group protein B1(HMGB1)and heat shock protein 70(HSP 70)***,a range of immune responses are activated to protect mice from the attack of cancer cells in vivo.

关键词： immunogenic cell death endoplasmic reticulum oxidative stress aggregation-induced emission reactive oxygen species

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hydrogenase as the basis for green hydrogen production and utilization

引用

Journal of Energy Chemistry 2023年第10期85卷 348-362,I0011页

作者： Haishuo Ji Lei Wan Yanxin Gao Ping Du Wenjin Li Hang Luo Jiarui Ning Yingying Zhao Huangwei Wang Lixin Zhang Liyun Zhang state key laboratory of medicinal chemical biology College of PharmacyNankai UniversityTianjin 300350China Max Planck Institute for chemical Energy Conversion Stiftstrasse 34-3645470 Mülheim an der RuhrGermany state key laboratory of medicinal chemical biology College of Life SciencesNankai UniversityTianjin 300350China state key laboratory of Bioreactor Engineering and School of BiotechnologyEast China University of Science and Technology(ECUST)Shanghai 200237China

Hydrogenase is a paradigm of highly efficient biocatalyst for H_(2) production and utilization evolved in nature. A dilemma is that despite the high activity and efficiency expected for hydrogenases as promising catalysts for the hydrogen economy, the poor oxygen tolerance and low yield of hydrogenases largely hinder their practical application. In these years, the enigmas surrounding hydrogenases regarding their structures, oxygen tolerance, mechanisms for catalysis, redox intermediates, and proton-coupled electron transfer schemes have been gradually elucidated;the schemes, which can well couple hydrogenases with other highly efficient(in)organic and biological catalysts to build novel reactors and drive valuable reactions, make it possible for hydrogenases to find their niches. To see how scientists put efforts to tackle this issue and design novel reactors in the fields where hydrogenases play crucial roles, in this review,recent advances were summarized, including different strategies for protecting enzyme molecules from oxygen, enzyme-based assembling systems for H_(2) evolution in the photoelectronic catalysis, enzymatic biofuel cells for H_(2) utilization and storage and the efficient electricity-hydrogen-carbohydrate cycle for high-purity hydrogen and biofuel automobiles. Limitations and future perspectives of hydrogenasebased applications in H_(2) production and utilization with great impact are discussed. In addition, this review also provides a new perspective on the use of biohydrogen in healthcare beyond energy.

关键词： Hydrogenase Biological H_(2)energy Oxygen tolerance Artificial photosynthesis Biofuel cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Primary cilia in corneal development and disease

引用

Zoological Research 2020年第5期41卷 495-502页

作者： Ting Song Jun Zhou Institute of Biomedical sciences Shandong Provincial Key Laboratory of Animal Resistance BiologyCollaborative Innovation Center of Cell Biology in Universities of ShandongCollege of Life SciencesShandong Normal UniversityJinanShandong 250014China state key laboratory of medicinal chemical biology College of Life SciencesNankai UniversityTianjin 300071China

As a transparent avascular tissue located at the front of the eyeball, the cornea is an important barrier to external damage. Both epithelial and endothelial cells of the cornea harbor primary cilia, which sense changes in the external environment and regulate intracellular signaling pathways. Accumulating evidence suggests that the primary cilium regulates corneal development in several ways, including participation in corneal epithelial stratification and maintenance of corneal endothelial cell *** addition, the primary cilium has been implicated in the pathogenesis of several corneal diseases. In this review, we discuss recent findings that demonstrate the critical role of the primary cilium in corneal development. We also discuss the link between ciliary dysfunction and corneal diseases, which suggests that the primary cilium could be targeted to treat these diseases.

关键词： Cornea Development Disease Cilium Epithelium Endothelium Treatment

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Reading Time and DNA Sequence Preference of TET3 CXXC Domain Revealed by Single-Molecule Profiling

引用

Chinese Journal of Chemistry 2023年第10期41卷 1177-1184页

作者： Zeyu Wang Zhiqiang Cao Kangkang Ma Man Lu Meije Wang Han Gao Deshun Gong Lin Liang Zhongbo Yu Frontiers Science Center for Cell Responses State Key Laboratory of Medicinal Chemical Biologyand College of PharmacyNankai University38 Tongyan RoadTianjin 300350China state key laboratory of medicinal chemical biology and college of life sciences Nankai UniversityTianjin 300350China college of Animal Science Shanxi Agricultural UniversityTaiguShanxi 030801China

Recognition of CpG dinucleotide DNA in epigenetic information flow plays a pivotal role for cellular differentiation and *** TET3 CXXC domain binds to CpG DNA,serving a basic epigenetic information reading *** the selective recognition of a CpG motif by a CXXC domain from crowded binding sites in a gene sequence,the protein-DNA interactions are beyond CpG ***,the selective binding dynamics of CpG within a long DNA context by epigenetic enzymes have been rarely exploit-ed,which is hard for ensemble methods to ***,we used single-molecule magnetic tweezers to quantitatively examine the dynamics of TET3's CXXC domain on a Hoxa9 promoter *** single-molecule binding profile revealed that CXXC-DNA interactions involve both CpG motifs and their flanking *** residence time of TET3 CXXC differs by about 1000 times in five distin-guished CpG clusters in the context of a CpG ***,we performed multi-state hidden Markov modeling analysis on the zip-ping/unzipping dynamics of a CpG hairpin,discovering TET3 CXXC's preference on CpG motifs regarding the-2 to+2 flanking *** results shed light on the selective binding dynamics of a CXXC on a gene sequence,facilitating studies on epigenetic information reading mechanisms.

关键词： Enzymes DNA recognition CpG flanking sequence Single-molecule studies Magnetic tweezers

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fast Determination of Phenanthrene in Soil by Gas Chroma- tography-Mass Spectrometry Using Chemometric Resolution and Standard Addition Method

引用

Chinese Journal of Chemistry 2013年第4期31卷 545-550页

作者： Weiwei Yu Wensheng Cai Xueguang Shao state key laboratory of medicinal chemical biology Research Center for Analytical Sciences College of Chemistry Nankai University Tianjin 300071 China

A method for fast determination of the component in complex samples by using gas chromatography-mass spectrometry （GC-MS） was developed and used for quantitative analysis of phenanthrene in soils. In the method, window independent component analysis （WICA） was used for resolving the mass spectrum and non-negative im- mune algorithm （NNIA） was employed for obtaining the chromatographic profile. Therefore, spectral and chroma- tographic information of a specific component can be obtained from the measured GC-MS data of overlapping and high background. Six soil samples collected from different places were analyzed. The tedious pretreatments in pre- paring the samples and the elution in the separation were simplified for speeding up the analysis. Due to the com- plexity of the matrix, standard addition method was adopted for the final quantification. The applicability of the method was validated with a spiked sample and the results of the six samples are reasonable.

关键词： phenanthrene gas chromatography-mass spectrometry standard addition method chemoinformatics immune algorithm

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Using selenium-conjugated polyethylene glycol to enhance the stability of gold nanoparticles in biologically relevant samples

引用

Science China Chemistry 2019年第2期62卷 280-286页

作者： Yang Xue Bo Dong Xuehui Liu Fengchao Wang Jie Yang Dingbin Liu state key laboratory of medicinal chemical biology Research Center for Analytical Sciences and Tianjin Key Laboratory of Molecular Recognition and Biosensing College of Chemistry Nankai University Collaborative Innovation Center of chemical Science and Engineering (Tianjin)

Using stabilizing agents to maintain the physicochemical properties of colloids in complex environments is crucial for their realworld applications. In this article, we describe how selenium-(Se-) terminated polyethylene glycol(PEG) can serve as a highaffinity stabilizing agent for gold nanoparticles(AuNPs). Compared to Au NPs modified with standard thiolated PEG(S-PEG),Se-PEG-coated Au NPs are much more stable under extreme conditions such as high/low pH, high salt content, and high temperatures. We demonstrate that the Se anchor can prevent the dissociation of PEG ligands from Au NP surfaces in living cells,where a higher concentration of biothiols is usually present. These results indicate that Se-PEG is an excellent stabilizing agent that may facilitate further studies on metal NPs for various complex and physiological systems.

关键词： selenium polyethylene glycol gold nanoparticles biological stability

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：