检索结果-南通市图书馆

Intracellular redox potential-driven live-cell synthesis of CdSe quantum dots in Staphylococcus aureus

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2024年第3期67卷 990-999页

作者： Cai-Qin Wu An-An Liu Xu Li Jia-Wei Tu Juan Kong Ling-Ling Yang Jian-Hong Jia Chuan Wang Bin Hu Zhi-Xiong Xie Dai-Wen Pang The Institute for Advanced Studies College of Chemistry and Molecular SciencesCollege of Life SciencesHubei Key Laboratory of Cell HomeostasisWuhan UniversityWuhan 430072China Research Center for Analytical sciences College of ChemistryState Key Laboratory of Medicinal Chemical BiologyTianjin Key Laboratory of Biosensing and Molecular RecognitionFrontiers Science Center for New Organic MatterFrontiers Science Center for Cell Responsesand Haihe Laboratory of Sustainable Chemical TransformationsNankai UniversityTianjin 300071China

Biosynthesized semiconductor quantum dots(QDs) have bright fluorescence,adjustable particle sizes,and environmental friendliness,endowing them with convenience and potential for biological *** to the unclear mechanism of the cellular environment on the live-cell synthesis of QDs,it is still difficult to regulate their optical ***,the critical role of the intracellular redox environment in regulating the fluorescence properties of biosynthesized CdSe QDs in Staphylococcus aureus(***) has been *** glutathione peroxidase(GPx) activity directly affects the intracellular H_(2)O_(2) produced in the SeO_(3)^(2-) reduction,which further manipulates the glutathione redox cycle to determine the content of low-valence *** a result,the fluorescence intensity of the synthesized CdSe QDs increases by 60% in the GPx overexpressed *** cellular redox potential that is controlled by the GSH redox cycle provides the driving force for the reduction of SeO_(3)^(2-),facilitating the synthesis of CdSe QDs in *** *** proposed mechanism of the cellular redox state provides a new perspective for regulating the synthesis of semiconductor nanomaterials in live cells.

关键词： redox H_(2)O_(2) peroxidase quantum dot Staphylococcus aureus

维普期刊数据库

Rational Design of Capping Ligands of Quantum Dots for Biosensing

在线全文

学校读者我要写书评

暂无评论

chemical Research in Chinese Universities 2024年第2期40卷 162-172页

作者： XU Xinran LIU An-an PANG Daiwen state key laboratory of medicinal chemical biology Tianjin Key Laboratory of Biosensing and Molecular RecognitionResearch Center for Analytical SciencesCollege of ChemistryNankai UniversityTianjin300071P.R.China

Quantum dots have been widely applied in biosensing due to their outstanding optical *** emissions of quantum dots are mainly determined by their composition and size,as described by the Brus’s ***,in this case,their emissions are hardly regulated reversibly and responsively,which are unsuitable for biosensing and *** the last decade,capping ligands have been used for designing biosensors because of their responsive regulation on the photoluminescence of quantum ***,we first summarize the advances in characterization and calculation specific for ligands,which have helped to provide insights into the photoluminescence process and energy band theory of quantum *** then review two ways of ligand design that influence the optical properties of quantum dots:affecting the process of photoluminescence,or the orbital/electronic *** the latter case,the atoms on both the ligand and the surface of the quantum dot interact to affect the energy band structure of the quantum dot *** are presented of how these quantum dots that possess responsive properties due to the design of the ligands have been applied to *** further exploration,we hope to see advances in the fundamental understanding of the energy band structures and practical applications of these quantum dots.

关键词： Quantum dot Ligand Photoluminescence Responsiveness Sensor

Pathologically relevant aldoses and environmental aldehydes cause cilium disassembly via formyl group-mediated mechanisms

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Molecular Cell biology 2024年第1期16卷 15-28页

作者： Te Li Min Liu Fan Yu Song Yang Weiwen Bu Kai Liu Jia Yang Hua Ni Mulin Yang Hanxiao Yin Renjie Hong Dengwen Li Huĳie Zhao Jun Zhou Haihe laboratory of Cell Ecosystem State Key Laboratory of Medicinal Chemical BiologyTianjin Key Laboratory of Protein ScienceCollege of Life SciencesNankai UniversityTianjin 300071China laboratory of Tissue Homeostasis Haihe Laboratory of Cell EcosystemTianjin 300462China Center for Cell Structure and Function Collaborative Innovation Center of Cell Biology in Universities of ShandongShandong Provincial Key Laboratory of Animal Resistance BiologyCollege of Life SciencesShandong Normal UniversityJinan 250014China

Carbohydrate metabolism disorders(CMDs),such as diabetes,galactosemia,and mannosidosis,cause ciliopathy-like multiorgan ***,the mechanistic link of cilia to CMD complications is still poorly ***,we describe significant cilium disassembly upon treatment of cells with pathologically relevant aldoses rather than the corresponding sugar ***,environmental aldehydes are able to trigger cilium disassembly by the steric hindrance effect of their formyl *** studies reveal that aldehydes stimulate extracellular calcium influx across the plasma membrane,which subsequently activates the calmodulin-Aurora A-histone deacetylase 6 pathway to deacetylate axonemal microtubules and triggers cilium *** vivo experiments further show that Hdac6 knockout mice are resistant to aldehyde-induced disassembly of tracheal cilia and sperm *** findings reveal a previously unrecognized role for formyl group-mediated cilium disassembly in the complications of CMDs.

关键词： carbohydrate metabolism disorder aldose aldehyde cilium disassembly formyl group calcium influx HDAC6

In-situ synthesis of quantum dots in the nucleus of live cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

National Science Review 2024年第3期11卷 172-182页

作者： Yusi Hu Zhi-Gang Wang Haohao Fu Chuanzheng Zhou Wensheng Cai Xueguang Shao Shu-Lin Liu Dai-Wen Pang state key laboratory of medicinal chemical biology Frontiers Science Centre for New Organic MatterTianjin Key Laboratory of Biosensing and Molecular Recognition Research Centre for Analytical Sciences College of Chemistry School of Medicine and Frontiers Science Centre for Cell Responses Nankai University Haihe laboratory of Sustainable chemical Transformations

The cell nucleus is the main site for the storage and replication of genetic material, and the synthesis of substances in the nucleus is rhythmic, regular and strictly regulated by physiological processes. However,whether exogenous substances, such as nanoparticles, can be synthesized in situ in the nucleus of live cells has not been reported. Here, we have achieved in-situ synthesis of CdSxSe1-xquantum dots（QDs） in the nucleus by regulation of the glutathione（GSH） metabolic pathway. High enrichment of GSH in the nucleus can be accomplished by the addition of GSH with the help of the Bcl-2 protein. Then, high-valence Se is reduced to low-valence Se by glutathione-reductase-catalyzed GSH, and interacts with the Cd precursor formed through Cd and thiol-rich proteins, eventually generating QDs in the nucleus. Our work contributes to a new understanding of the syntheses of substances in the cell nucleus and will pave the way for the development of advanced ‘supercells’.

关键词： glutathione live cell nucleus quantum dot intracellular synthesis

Discovery of antitumor diterpenoids from Casearia graveolens targeting VEGFR-2 to inhibit angiogenesis

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2024年第9期22卷 842-853页

作者： WANG Sibei LIU Yuhui LIANG Yue XI Yaru ZHAI Yupeng LEE Dongho XU Jing GUO Yuanqiang state key laboratory of medicinal chemical biology College of Pharmacyand Tianjin Key Laboratory of Molecular Drug ResearchNankai UniversityTianjin 300350China state key laboratory of Functions and Applications of medicinal Plants Guizhou Medical UniversityGuiyang 550014China Department of Plant Biotechnology College of Life Sciences and BiotechnologyKorea UniversitySeoul 02841South Korea

Eight novel clerodane diterpenoids(1-8)were isolated from the twigs of Casearia *** structures were elucidated through comprehensive nuclear magnetic resonance(NMR),high-resolution electrospray ionization mass spectrometry(HRESI-MS),and electronic circular dichroism(ECD)*** addition to structural determination,surface plasmon resonance(SPR)assays were conducted to investigate molecular interactions,revealing that compound 8 exhibited high affinity for vascular endothelial growth factor receptor 2(VEGFR2),a key regulator of tumor *** in vivo experiments demonstrated that compound 8 effectively inhibited angiogenesis and displayed significant antitumor activity by suppressing tumor proliferation and metastasis in zebrafish xenograft *** findings suggest that compound 8 holds promise as an anticancer lead compound targeting VEGFR-2 to obstruct tumor angiogenesis.

关键词： Clerodane diterpenoids Casearia graveolens Surface plasmon resonance Vascular endothelial growth factor receptor 2 Antitumor activity Zebrafish

Förster resonance energy transfer reveals phillygenin and swertiamarin concurrently target AKT on different binding domains to increase the anti-inflammatory effect

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese chemical Letters 2024年第5期35卷 207-212页

作者： Xiaoyao Ma Jinling Zhang Ge Fang He Gao Jie Gao Li Fu Yuanyuan Hou Gang Bai state key laboratory of medicinal chemical biology College of Pharmacy and Tianjin Key Laboratory of Molecular Drug ResearchNankai UniversityTianjin 300000China college of life Health Dalian UniversityDalian 116622China Dalian Fusheng Pharmaceutical Co. Ltd.Dalian 116000China

The clinical benefit of combination therapy is significant,but it is not easy to define the mechanism of complexity and *** studies illustrate that phillygenin(Phi)binds in the allosteric inhibit pocket of protein kinase B(AKT),and swertiamarin(Swe)acts on the pleckstrin homology(PH)domain of ***,the combined synergistic effect of relieving the inflammatory response has yet to be *** on high sensitivity,specificity and fast-responsibility fluorescent sensors,the Förster resonance energy transfer(FRET)technique offers a route to provide clear insights into physiological and pathological *** the study,molecular docking,the fluorescent probes of Phi and Swe for FRET were designed and *** analysis shown that Swe and Phi concurrently acted on the PH domain and allosterically inhibited pocket of AKT,*** combination of Swe and Phi significantly increased the heat stability of AKT and decreased protease-induced *** lipopolysaccharides(LPS)-induced mice and cells,the combination arrested AKT activation,nuclear factor kappa-B(NF-κB)phosphorylation,and the expression of tumor necrosis factor-α(TNF-α),interleukin(IL)-6 and *** conclusion,FRET revealed Phi and Swe concurrently targeted AKT on different domains and the combination of Phi and Swe enhanced the anti-inflammatory effect.

关键词： FRET Swertiamarin Phillygenin AKT Anti-inflammation Combinatorial drug

Discovery of antitumor diterpenoids from Casearia graveolens targeting VEGFR-2 to inhibit angiogenesis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2024年第09期 842-853页

作者： WANG Sibei LIU Yuhui LIANG Yue XI Yaru ZHAI Yupeng LEE Dongho XU Jing GUO Yuanqiang state key laboratory of medicinal chemical biology College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research Nankai University Department of Plant Biotechnology College of Life Sciences and Biotechnology Korea University state key laboratory of Functions and Applications of medicinal Plants Guizhou Medical University

Eight novel clerodane diterpenoids(1-8) were isolated from the twigs of Casearia graveolens. Their structures were elucidated through comprehensive nuclear magnetic resonance(NMR), high-resolution electrospray ionization mass spectrometry(HRESI-MS), and electronic circular dichroism(ECD) analyses. In addition to structural determination, surface plasmon resonance(SPR)assays were conducted to investigate molecular interactions, revealing that compound 8 exhibited high affinity for vascular endothelial growth factor receptor 2(VEGFR2), a key regulator of tumor angiogenesis. Subsequent in vivo experiments demonstrated that compound 8 effectively inhibited angiogenesis and displayed significant antitumor activity by suppressing tumor proliferation and metastasis in zebrafish xenograft models. These findings suggest that compound 8 holds promise as an anticancer lead compound targeting VEGFR-2 to obstruct tumor angiogenesis.

关键词：

Primary cilia restrict autoinflammation by mediating PD-L1 expression

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science Bulletin 2024年第16期69卷 2505-2508页

作者： Xiaoyu Tian Kaiyue Zhang Renjie Hong Hanyu Wang Xifeng Dong Jun Zhou Yunfan Yang Min Liu Center for Cell Structure and Function Shandong Provincial Key Laboratory of Animal Resistance BiologyHaihe Laboratory of Cell EcosystemCollaborative Innovation Center of Cell Biology in Universities of ShandongCollege of Life SciencesShandong Normal UniversityJinan 250014China state key laboratory of medicinal chemical biology College of Life SciencesNankai UniversityTianjin 300071China Department of Hematology Tianjin Medical University General HospitalTianjin 300052China Department of Cell biology School of Basic Medical SciencesCheeloo College of MedicineShandong UniversityJinan 250012China laboratory of Tissue Homeostasis Haihe Laboratory of Cell EcosystemTianjin 300462China

Ciliopathies are multisystem disorders characterized by the dysfunction of motile and/or non-motile cilia,which are microtubule-based structures protruding from the cell surface and function in cell motility and *** clinical manifestations of ciliopathies include retinal degeneration,mental retardation,renal abnormality,obesity,and skeletal dysplasia[1,2].Fibrosis of vital organs,characterized by the extensive deposition of extracellular matrix components,represents another complication frequently observed in patients and animal models of ciliopathies[3].

关键词： inflammation organs degeneration

维普期刊数据库

Stay in touch with the endoplasmic reticulum

在线全文

学校读者我要写书评

暂无评论

Science China(life sciences) 2024年第2期67卷 230-257页

作者： Sha Sun Gan Zhao Mingkang Jia Qing Jiang Shulin Li Haibin Wang Wenjing Li Yunyun Wang Xin Bian Yan G.Zhao Xun Huang Ge Yang Huaqing Cai Jose C.Pastor-Pareja Liang Ge Chuanmao Zhang Junjie Hu National laboratory of Biomacromolecules Institute of BiophysicsUniversity of Chinese Academy of SciencesChinese Academy of SciencesBeijing 100101China The Ministry of Education key laboratory of Cell Proliferation and Differentiation College of Life SciencesPeking UniversityBeijing 100871China state key laboratory of Membrane biology Tsinghua-Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China laboratory of Computational biology&Machine Intelligence School of Artificial IntelligenceUniversity of Chinese Academy of SciencesBeijing 100049China state key laboratory of medicinal chemical biology College of Life SciencesNankai UniversityTianjin 300071China Brain Research Center Department of BiologySchool of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China state key laboratory of Molecular Developmental biology Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China Institute of Neurosciences Consejo Superior de Investigaciones Científicas-Universidad Miguel HernándezSan Juan de Alicante 03550Spain

The endoplasmic reticulum(ER),which is composed of a continuous network of tubules and sheets,forms the most widely distributed membrane system in eukaryotic *** a result,it engages a variety of organelles by establishing membrane contact sites(MCSs).These contacts regulate organelle positioning and remodeling,including fusion and fission,facilitate precise lipid exchange,and couple vital signaling ***,we systematically review recent advances and converging themes on ER-involved organellar *** molecular basis,cellular influence,and potential physiological functions for ER/nuclear envelope contacts with mitochondria,Golgi,endosomes,lysosomes,lipid droplets,autophagosomes,and plasma membrane are summarized.

关键词： endoplasmic reticulum nuclear envelope mitochondria Golgi apparatus endosome lysosome lipid droplets autophagosome plasma membrane membrane contact site

维普期刊数据库